Short regulatory DNA sequences to target brain endothelial cells for gene therapy

Short regulatory DNA sequences to target brain endothelial cells for gene therapy

Gene vectors focusing on CNS endothelial cells enable to govern the blood-brain barrier and to right genetic defects within the CNS. As a result of vectors based mostly on the adeno-associated virus (AAV) have a restricted capability, it’s important that the DNA sequence controlling gene expression is brief. As well as, it should be particular […]

Oligochitosan modified albumin as plasmid DNA delivery vector: endocytic trafficking, polyplex fate, in vivo compatibility.

Cationic macromolecules condense DNA into small nanoparticles and kind polyplex. The composition of the polyplex determines the endocytic course of, the intracellular routing and the destiny of the polyplex. Beforehand, oligochitosan-modified vectors with completely different protein moieties are used as gene supply vector and the varieties of protein moiety can affect the endosome escape capability […]

Successful delivery of large-size CRISPR/Cas9 vectors in hard-to-transfect human cells using small plasmids

Successful delivery of large-size CRISPR/Cas9 vectors in hard-to-transfect human cells using small plasmids

With the rise of recent highly effective genome engineering applied sciences, equivalent to CRISPR/Cas9, cell fashions might be engineered successfully to speed up fundamental and illness analysis. Essentially the most essential step on this process is the environment friendly supply of international nucleic acids into cells by mobile transfection. For the reason that vectors encoding […]

Gene engineered mesenchymal stem cells: greater transgene expression and efficacy with minicircle vs. plasmid DNA vectors in a mouse model of acute lung injury

Gene engineered mesenchymal stem cells: greater transgene expression and efficacy with minicircle vs. plasmid DNA vectors in a mouse model of acute lung injury

 Acute lung damage (ALI) and in its extreme kind, acute respiratory misery syndrome (ARDS), ends in elevated pulmonary vascular irritation and permeability and is a serious reason for mortality in lots of critically sick sufferers. Though cell-based therapies have proven promise in experimental ALI, methods are wanted to reinforce the efficiency of mesenchymal stem cells […]

Preclinical safety evaluation of a recombinant plasmid vector encoding mature human neutrophil peptide-1 by repeated local administrations in nonhuman primates

Preclinical safety evaluation of a recombinant plasmid vector encoding mature human neutrophil peptide-1 by repeated local administrations in nonhuman primates

In our earlier research, a novel gene remedy method was developed based mostly on a plasmid vector pSecTag2B through which recombinant HNP1 gene was regulated below a cytomegalovirus promoter to encode a mature HNP1 type. We confirmed for the primary time in numerous tumor fashions together with human most cancers xenografts that overexpression of HNP1 […]