In our earlier research, a novel gene remedy method was developed based mostly on a plasmid vector pSecTag2B through which recombinant HNP1 gene was regulated below a cytomegalovirus promoter to encode a mature HNP1 type. We confirmed for the primary time in numerous tumor fashions together with human most cancers xenografts that overexpression of HNP1 within the tumor milieu by intratumoral pSecTag-HNP1 (pHNP1) administration effectively attenuated in vivo tumor development, mediated host immune responses to tumors, and produced a synergistic impact when mixed with chemotherapeutics.
In present research, a preclinical security investigation of HNP1 gene remedy was carried out in non-human primates. Eleven cynomolgus monkeys had been divided into three teams of three to four animals every and obtained both repeated s.c. injections of pHNP1/cationic liposome complexes at low (0.625 mg/kg) or excessive (2.5 mg/kg) dose or glucose as management. Important HNP1 in vivo accumulation was detected after consecutive administrations. All primates reached the top of the research with good physique situations. Injection web site irritation was the one apparent poisonous response throughout commentary interval.
As well as, elevation of monocyte/macrophage and neutrophil in addition to decline of lymphocyte had been detected within the peripheral blood of pHNP1-treated primates. These alterations had been partially alleviated on the finish of commentary interval. In addition to, dose-related histopathological adjustments of the immune organs had been noticed at necropsy, together with a minimal thymic lymphocyte lower and a minimal-to-mild lymph node erythrocyte enhance, however which can’t be excluded from HNP1 induced immune reactions. Collectively, these information help future medical research of pHNP1-based native gene supply in tumor sufferers.
The downregulation of endogenous dhfr improved the effectivity of EBNA-1 amplification, as evidenced by a comparability with the amplification extent in cells missing shRNA expression on the similar MTX focus. The EBNA-1 expression ranges from the EBNA-1-amplified clones chosen on this research had been larger than these obtained from EBNA-1-amplified clones that had been generated utilizing the standard amplification in our earlier research. According to earlier research, EBNA-1 amplification improved the manufacturing of the Fc-fusion protein by a selected protein productiveness (qp)-enhancing impact, slightly than by bettering cell development or transfection effectivity.
Joint common modular plasmids (JUMP): a versatile vector platform for artificial biology
Technology of latest DNA constructs is an important course of in trendy life science and biotechnology. Modular cloning techniques based mostly on Golden Gate cloning, utilizing Kind IIS restriction endonucleases, permit meeting of complicated multipart constructs from reusable fundamental DNA components in a fast, dependable and automation-friendly manner. Many such toolkits can be found, with various levels of compatibility, most of that are aimed toward particular host organisms. Right here, we current a vector design which permits easy vector modification by utilizing modular cloning to assemble and add new capabilities in secondary websites flanking the primary insertion web site (used for typical modular cloning).
Meeting in all websites is suitable with the PhytoBricks normal, and vectors are suitable with the Commonplace European Vector Structure (SEVA) in addition to BioBricks. We exhibit that this facilitates the development of vectors with tailor-made capabilities and simplifies the workflow for producing libraries of constructs with frequent components. We have now made out there a set of vectors with 10 totally different microbial replication origins, various in copy quantity and host vary, and permitting chromosomal integration, in addition to a choice of generally used fundamental components.
This design expands the vary of hosts which might be simply modified by modular cloning and acts as a toolkit which can be utilized to facilitate the technology of latest toolkits with particular capabilities required for focusing on additional hosts. The antigen produced by the described approach is appropriate for serological exams and subunit vaccine research. We developed a easy purification scheme that constantly yielded as much as 30 mg of RBD protein per liter of the easy shake flask cell tradition.
Excessive-level expression of the monomeric SARS-CoV-2 S protein RBD 320-537 in stably transfected CHO cells by the EEF1A1-based plasmid vector
The spike (S) protein is likely one of the three proteins forming the coronaviruses’ viral envelope. The S protein of the Extreme Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial construction much like the S proteins of different mammalian coronaviruses, aside from a singular receptor-binding area (RBD), which is a big inducer of host immune response. Recombinant SARS-CoV-2 RBD is broadly used as a extremely particular minimal antigen for serological exams.
Right publicity of antigenic determinants has a big affect on the accuracy of such tests-the antigen must be appropriately folded, comprise no probably antigenic non-vertebrate glycans, and, ideally, ought to have a glycosylation sample much like the native S protein. Based mostly on the beforehand developed p1.1 vector, containing the regulatory sequences of the Eukaryotic translation elongation issue 1 alpha gene (EEF1A1) from Chinese language hamster, we created two expression constructs encoding SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine tags.
Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 418 |
Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 575 |
Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 429 |
Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 591 |
Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-p-48Tests | Reddot Biotech | 48 Tests | EUR 494 |
Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-p-96Tests | Reddot Biotech | 96 Tests | EUR 684 |
Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 450 |
Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 622 |
Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-Rb-48Tests | Reddot Biotech | 48 Tests | EUR 450 |
Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-Rb-96Tests | Reddot Biotech | 96 Tests | EUR 622 |
Bovine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-b-48T | DL Develop | 48T | EUR 493 |
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Description: A sandwich quantitative ELISA assay kit for detection of Bovine Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Bovine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-b-96T | DL Develop | 96T | EUR 641 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Canine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-c-48T | DL Develop | 48T | EUR 474 |
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Description: A sandwich quantitative ELISA assay kit for detection of Canine Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma or other biological fluids. |
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Canine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-c-96T | DL Develop | 96T | EUR 614 |
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Description: A sandwich quantitative ELISA assay kit for detection of Canine Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma or other biological fluids. |
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Chicken Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-Ch-48T | DL Develop | 48T | EUR 454 |
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Description: A sandwich quantitative ELISA assay kit for detection of Chicken Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Chicken Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-Ch-96T | DL Develop | 96T | EUR 587 |
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Description: A sandwich quantitative ELISA assay kit for detection of Chicken Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Goat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-g-48T | DL Develop | 48T | EUR 503 |
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Description: A sandwich quantitative ELISA assay kit for detection of Goat Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Goat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-g-96T | DL Develop | 96T | EUR 654 |
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Description: A sandwich quantitative ELISA assay kit for detection of Goat Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-Hu-48T | DL Develop | 48T | EUR 425 |
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Description: A sandwich quantitative ELISA assay kit for detection of Human Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-Hu-96T | DL Develop | 96T | EUR 548 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-Mu-48T | DL Develop | 48T | EUR 435 |
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Description: A sandwich quantitative ELISA assay kit for detection of Mouse Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-Mu-96T | DL Develop | 96T | EUR 561 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
|||
Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
|||
| DLR-BMP4-p-48T | DL Develop | 48T | EUR 493 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-p-96T | DL Develop | 96T | EUR 641 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-Ra-48T | DL Develop | 48T | EUR 454 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-Ra-96T | DL Develop | 96T | EUR 587 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-Rb-48T | DL Develop | 48T | EUR 454 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
|||
| DLR-BMP4-Rb-96T | DL Develop | 96T | EUR 587 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates or other biological fluids. |
|||
Bovine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
|||
| RDR-BMP4-b-48Tests | Reddot Biotech | 48 Tests | EUR 516 |
Bovine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
|||
| RDR-BMP4-b-96Tests | Reddot Biotech | 96 Tests | EUR 716 |
Canine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
|||
| RDR-BMP4-c-48Tests | Reddot Biotech | 48 Tests | EUR 493 |
Canine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
|||
| RDR-BMP4-c-96Tests | Reddot Biotech | 96 Tests | EUR 683 |
Chicken Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-Ch-48Tests | Reddot Biotech | 48 Tests | EUR 470 |
Chicken Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-Ch-96Tests | Reddot Biotech | 96 Tests | EUR 651 |
Goat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-g-48Tests | Reddot Biotech | 48 Tests | EUR 528 |
Goat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-g-96Tests | Reddot Biotech | 96 Tests | EUR 733 |
Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 436 |
Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
|||
| RDR-BMP4-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 601 |
Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
|||
| RDR-BMP4-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 447 |
Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
|||
| RDR-BMP4-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 618 |
Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-p-48Tests | Reddot Biotech | 48 Tests | EUR 516 |
Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
|||
| RDR-BMP4-p-96Tests | Reddot Biotech | 96 Tests | EUR 716 |
Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 470 |
Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
|||
| RDR-BMP4-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 651 |
Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
|||
| RDR-BMP4-Rb-48Tests | Reddot Biotech | 48 Tests | EUR 470 |
Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-Rb-96Tests | Reddot Biotech | 96 Tests | EUR 651 |
HEK-293T Telomerase Over-Expressing Cell Pellet |
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| abx069991-1Pellet | Abbexa | 1 Pellet | EUR 398 |
Beaucage reagent |
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| HY-100951 | MedChemExpress | 10mM/1mL | EUR 126 |
BOP reagent |
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| 5-02141 | CHI Scientific | 25g | Ask for price |
BOP reagent |
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| 5-02142 | CHI Scientific | 100g | Ask for price |
Bradford reagent |
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| BDE641 | Bio Basic | 100ml | EUR 61.01 |
BOP reagent |
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| A7015-100000 | ApexBio | 100 g | EUR 200 |
|
Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis. |
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BOP reagent |
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| A7015-25000 | ApexBio | 25 g | EUR 113 |
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Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis. |
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Bluing Reagent |
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| BRT030 | ScyTek Laboratories | 30 ml | EUR 60 |
Bluing Reagent |
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| BRT125 | ScyTek Laboratories | 125 ml | EUR 63 |
Bluing Reagent |
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| BRT3800 | ScyTek Laboratories | 1 Gal. | EUR 184 |
Bluing Reagent |
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| BRT500 | ScyTek Laboratories | 500 ml | EUR 76 |
Bluing Reagent |
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| BRT999 | ScyTek Laboratories | 1000 ml | EUR 88 |
Chymase reagent |
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| 30C-CP1129 | Fitzgerald | 5 units | EUR 2185 |
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Description: Purified native Human Chymase reagent |
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Traut's Reagent |
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| 2330-1000 | Biovision | EUR 349 | |
Traut's Reagent |
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| 2330-500 | Biovision | EUR 207 | |
MTS Reagent |
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| 2808-1000 | Biovision | EUR 990 | |
MTS Reagent |
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| 2808-250 | Biovision | EUR 365 | |
MTT Reagent |
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| 2809-1G | Biovision | EUR 180 | |
MTT Reagent |
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| 2809-5G | Biovision | EUR 544 | |
BMP4 Antibody |
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| 1-CSB-PA212919 | Cusabio |
|
|
|
Description: A polyclonal antibody against BMP4. Recognizes BMP4 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:30-1:150 |
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BMP4 Antibody |
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| 1-CSB-PA002740GA01HU | Cusabio |
|
|
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Description: A polyclonal antibody against BMP4. Recognizes BMP4 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB |
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BMP4 Antibody |
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| 1-CSB-PA05799A0Rb | Cusabio |
|
|
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Description: A polyclonal antibody against BMP4. Recognizes BMP4 from Human, Mouse, Rat, Zebrafish. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:100-1:1000 |
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BMP4 Antibody |
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| 1-CSB-PA763132 | Cusabio |
|
|
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Description: A polyclonal antibody against BMP4. Recognizes BMP4 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:25-1:100 |
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BMP4 Antibody |
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| 35652-100ul | SAB | 100ul | EUR 252 |
BMP4 siRNA |
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| 20-abx909174 | Abbexa |
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BMP4 siRNA |
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| 20-abx909175 | Abbexa |
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BMP4 antibody |
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| 38256-100ul | SAB | 100ul | EUR 252 |
BMP4 Antibody |
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| 43852-100ul | SAB | 100ul | EUR 252 |
BMP4 Antibody |
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| ABD6461 | Lifescience Market | 100 ug | EUR 438 |
BMP4 Antibody |
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| ABF5175 | Lifescience Market | 100 ug | EUR 438 |
BMP4 Antibody |
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| DF6461 | Affbiotech | 200ul | EUR 304 |
|
Description: BMP4 Antibody detects endogenous levels of total BMP4. |
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BMP4 Antibody |
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| BF0696 | Affbiotech | 200ul | EUR 376 |
|
Description: BMP4 antibody detects endogenous levels of total BMP4. |
|||
anti-BMP4 |
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| LF-PA0093 | Abfrontier | 100 ul | EUR 334 |
|
Description: Rabbit polyclonal to BMP4 |
|||
BMP4 antibody |
|||
| 10R-B119a | Fitzgerald | 100 ug | EUR 792 |
|
Description: Mouse monoclonal BMP4 antibody |
|||
BMP4 antibody |
|||
| 10R-B119B | Fitzgerald | 500 ug | EUR 273 |
|
Description: Mouse monoclonal BMP4 antibody |
|||
BMP4 antibody |
|||
| 10R-2148 | Fitzgerald | 100 ul | EUR 403 |
|
Description: Mouse monoclonal BMP4 antibody |
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BMP4 antibody |
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| 10R-3460 | Fitzgerald | 100 ul | EUR 691 |
|
Description: Mouse monoclonal BMP4 antibody |
|||
BMP4 antibody |
|||
| 70R-16011 | Fitzgerald | 50 ul | EUR 435 |
|
Description: Rabbit polyclonal BMP4 antibody |
|||
BMP4 Antibody |
|||
| AF5175 | Affbiotech | 200ul | EUR 304 |
|
Description: BMP4 Antibody detects endogenous levels of total BMP4. |
|||
BMP4 antibody |
|||
| 20R-2936 | Fitzgerald | 100 ul | EUR 393 |
|
Description: Rabbit polyclonal BMP4 antibody |
|||
BMP4 antibody |
|||
| 70R-12408 | Fitzgerald | 100 ug | EUR 403 |
|
Description: Rabbit polyclonal BMP4 antibody |
|||
BMP4 antibody |
|||
| 70R-12409 | Fitzgerald | 100 ug | EUR 403 |
|
Description: Rabbit polyclonal BMP4 antibody |
|||
BMP4 protein |
|||
| 30R-1255 | Fitzgerald | 100 ug | EUR 224 |
|
Description: Purified recombinant Human BMP4 protein |
|||
BMP4 protein |
|||
| 30R-2651 | Fitzgerald | 10 ug | EUR 341 |
|
Description: Purified recombinant Human BMP4 protein |
|||
BMP4 protein |
|||
| 30R-AB029 | Fitzgerald | 5 ug | EUR 273 |
|
Description: Purified recombinant Human BMP4 protein |
|||
pENTR223- BMP4 |
|||
| PVT10009 | Lifescience Market | 2 ug | EUR 266 |
pCDNA3.1- BMP4 |
|||
| PVT10207 | Lifescience Market | 2 ug | EUR 301 |
BCA Reagent, 16ML |
|||
| C144-16ML | Arbor Assays | 16ML | EUR 163 |
Dissociation Reagent, 1ML |
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| X017-1ML | Arbor Assays | 1ML | EUR 109 |
Dissociation Reagent, 25ML |
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| X017-25ML | Arbor Assays | 25ML | EUR 258 |
Dissociation Reagent, 5ML |
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| X017-5ML | Arbor Assays | 5ML | EUR 122 |
Dissociation Reagent, 1ML |
|||
| X058-1ML | Arbor Assays | 1ML | EUR 73 |
Dissociation Reagent, 5ML |
|||
| X058-5ML | Arbor Assays | 5ML | EUR 109 |
Biolipidure-1002-Reagent |
|||
| Biolipidure-1002-10 | Cusabio | 10mL | EUR 196 |
|
Description: The Biolipidure-1002-Reagent is a synthetic amphoteric polymer that can be substituted for BSA in tubidimetric immunoassays. Biolipidure-1002 is an excellent blocker and also enhances assay sensitivity. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
|||
HighGene transfection reagent |
|||
| RM09014 | Abclonal | 1000μl | EUR 270 |
LP4K Transfection Reagent |
|||
| LP4K | GenTarget | 1.0 ml / vial | EUR 304 |
|
Description: Lipid based transfection reagent for large plasmid and multiple plasmid transfection in both adhesive and suspenstion cell types. |
|||
n-Heptane Reagent |
|||
| HC5400 | Bio Basic | 1L | EUR 79 |
Tri-RNA Reagent |
|||
| FATRR-001 | Favorgen | 100ml | EUR 236 |
Tri-RNA Reagent |
|||
| FATRR-002 | Favorgen | 50ml | EUR 176 |
Tri-RNA Reagent |
|||
| FATRR-003 | Favorgen | 450ml | EUR 645 |
DTT (Cleland's reagent) |
|||
| DB0058 | Bio Basic | 5g | EUR 84.8 |
DTNB (Ellman's Reagent) |
|||
| DB0113 | Bio Basic | 5g | EUR 97.85 |
Ethyl acetate Reagent |
|||
| EC4600 | Bio Basic | 1L | EUR 79 |
TissueDigest Reagent, 20X |
|||
| T101 | Insitus Biotechnologies | 10ml | EUR 210 |
Convoy? Transfection Reagent |
|||
| 1110-1ml | ACTGene | EUR 341 | |
Griess Reagent Kit |
|||
| 30100 | Biotium | 1KIT | EUR 149 |
|
Description: Minimum order quantity: 1 unit of 1KIT |
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PhosphoBlocker Blocking Reagent |
|||
| AKR-103 | Cell Biolabs | 1L | EUR 328 |
|
Description: Most commercially available Western blot blockers, such as dry milk or serum, are sufficient to block unreactive sites on the membrane. However, they are not designed to preserve phosphoprotein antigens during blotting. Our PhosphoBLOCKER Blocking Reagent provides superior blocking by maximizing signal-to-noise ratio. The PhosphoBLOCKER reagent particluarly excels with very low levels of endogenous phopsphoproteins. |
|||
PhosphoBlocker Blocking Reagent |
|||
| AKR-104 | Cell Biolabs | 4L | EUR 711 |
|
Description: Most commercially available Western blot blockers, such as dry milk or serum, are sufficient to block unreactive sites on the membrane. However, they are not designed to preserve phosphoprotein antigens during blotting. Our PhosphoBLOCKER Blocking Reagent provides superior blocking by maximizing signal-to-noise ratio. The PhosphoBLOCKER reagent particluarly excels with very low levels of endogenous phopsphoproteins. |
|||
EL Transfection Reagent |
|||
| 20-abx098880 | Abbexa |
|
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Mycoplasma Prevention Reagent |
|||
| 20-abx098886 | Abbexa |
|
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Girard's reagent T |
|||
| 20-abx184099 | Abbexa |
|
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FcR blocking Reagent |
|||
| 20-abx290024 | Abbexa |
|
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Detection Reagent A |
|||
| abx296004-120ul | Abbexa | 120 ul | EUR 321 |
Mycoplasma Prevention Reagent |
|||
| 20-abx298005 | Abbexa |
|
|
Biolipidure-1002-Reagent |
|||
| Biolipidure-1002-100 | Biolipidure | 100mL | EUR 1223 |
|
Description: The Biolipidure-1002-Reagent is a synthetic amphoteric polymer that can be substituted for BSA in tubidimetric immunoassays. Biolipidure-1002 is an excellent blocker and also enhances assay sensitivity. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
|||
Biolipidure-103-Reagent |
|||
| Biolipidure-103-10 | Biolipidure | 10mL | EUR 196 |
|
Description: The Biolipidure-103-Reagent is a synthetic amphoteric polymer that can be substituted for BSA. It has been shown to enhance signals in rapid tests, western blots, and other similar immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
|||
Biolipidure-103-Reagent |
|||
| Biolipidure-103-100 | Biolipidure | 100mL | EUR 1223 |
|
Description: The Biolipidure-103-Reagent is a synthetic amphoteric polymer that can be substituted for BSA. It has been shown to enhance signals in rapid tests, western blots, and other similar immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
|||
Biolipidure-1201-Reagent |
|||
| Biolipidure-1201-10 | Biolipidure | 10mL | EUR 196 |
|
Description: The Biolipidure-1201 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-1201-Reagent |
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| Biolipidure-1201-100 | Biolipidure | 100mL | EUR 1223 |
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Description: The Biolipidure-1201 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-1301-Reagent |
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| Biolipidure-1301-10 | Biolipidure | 10mL | EUR 196 |
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Description: The Biolipidure-1301 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-1301-Reagent |
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| Biolipidure-1301-100 | Biolipidure | 100mL | EUR 1223 |
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Description: The Biolipidure-1301 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-203-Reagent |
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| Biolipidure-203-10 | Biolipidure | 10mL | EUR 196 |
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Description: The Biolipidure-203 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-203 has been shown to enhance signal strength by improving signal-to-noise in ELISAs, EIAs, and related immunoassays. It also functions as an effective blocker and stabilizer in these assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-203-Reagent |
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| Biolipidure-203-100 | Biolipidure | 100mL | EUR 1223 |
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Description: The Biolipidure-203 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-203 has been shown to enhance signal strength by improving signal-to-noise in ELISAs, EIAs, and related immunoassays. It also functions as an effective blocker and stabilizer in these assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-206-Reagent |
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| Biolipidure-206-10 | Biolipidure | 10mL | EUR 196 |
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Description: The Biolipidure-206 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-206 enhances signal strength, functions as an effective blocker, and stabilizes proteins and antibodies in ELISAs, EIAs, and related immunoassays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-206-Reagent |
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| Biolipidure-206-100 | Biolipidure | 100mL | EUR 1223 |
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Description: The Biolipidure-206 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-206 enhances signal strength, functions as an effective blocker, and stabilizes proteins and antibodies in ELISAs, EIAs, and related immunoassays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-405-Reagent |
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| Biolipidure-405-10 | Biolipidure | 10mL | EUR 196 |
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Description: The Biolipidure-405 Reagent is a synthetic anionic polymer that can be used to enhance immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-405-Reagent |
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| Biolipidure-405-100 | Biolipidure | 100mL | EUR 1223 |
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Description: The Biolipidure-405 Reagent is a synthetic anionic polymer that can be used to enhance immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-502-Reagent |
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| Biolipidure-502-10 | Biolipidure | 10mL | EUR 196 |
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Description: The Biolipidure-502 Reagent is a synthetic cationic polymer. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-502-Reagent |
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| Biolipidure-502-100 | Biolipidure | 100mL | EUR 1223 |
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Description: The Biolipidure-502 Reagent is a synthetic cationic polymer. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-702-Reagent |
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| Biolipidure-702-10 | Biolipidure | 10mL | EUR 196 |
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Description: The Biolipidure-702 Reagent is a synthetic amphoteric polymer. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-702-Reagent |
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| Biolipidure-702-100 | Biolipidure | 100mL | EUR 1223 |
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Description: The Biolipidure-702 Reagent is a synthetic amphoteric polymer. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-802-Reagent |
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| Biolipidure-802-10 | Biolipidure | 10mL | EUR 196 |
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Description: The Biolipidure-802 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-802 generally enhances signal strength, functions as an effective blocker, and stabilizes proteins and antibodies in ELISAs, EIAs, Rapid-test, and related immunoassays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-802-Reagent |
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| Biolipidure-802-100 | Biolipidure | 100mL | EUR 1223 |
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Description: The Biolipidure-802 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-802 generally enhances signal strength, functions as an effective blocker, and stabilizes proteins and antibodies in ELISAs, EIAs, Rapid-test, and related immunoassays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Alcohol, Reagent (70%) |
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| EAS500 | ScyTek Laboratories | 500 ml | EUR 79 |
Alcohol, Reagent (70%) |
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| EAS999 | ScyTek Laboratories | 1000 ml | EUR 101 |
PureFection Transfection Reagent |
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| LV750A-1 | SBI | 1 ml | EUR 359 |
Biotin reagent (HRP) |
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| 65C-CE0202 | Fitzgerald | 5 mg | EUR 244 |
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Description: HRP conjugated biotin labelling reagent |
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BSA (Reagent Grade) |
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| 30-AB79 | Fitzgerald | 1 kg | EUR 1552 |
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Description: Reagent Grade Bovine Serum Albumin (99% pure) |
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BSA (Reagent Grade) |
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| 30-AB81 | Fitzgerald | 200 grams | EUR 476 |
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Description: Reagent Grade Sulphydryl Blocked BSA (99% pure) |
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HAMA blocking reagent |
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| 85R-1001 | Fitzgerald | 1 gram | EUR 1974 |
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Description: HAMA Blocking Reagent for use in immunoassays such as ELISA |
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HAMA blocking reagent |
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| 85R-1001P | Fitzgerald | 1 gram | EUR 2190 |
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Description: HAMA Blocking Reagent for use in immunoassays such as ELISA |
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HAMA blocking reagent |
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| 85R-1003 | Fitzgerald | 1 gram | EUR 1974 |
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Description: HAMA Blocking Reagent for use in immunoassays such as Rapid Tests |
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HAMA blocking reagent |
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| 85R-1014 | Fitzgerald | 50 mg | EUR 192 |
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Description: HAMA blocking reagent for use in assays specific for clinical false positive samples |
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HAMA blocking reagent |
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| 85R-1025 | Fitzgerald | 50 mg | EUR 192 |
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Description: HAMA blocking reagent for use in immunoassays |
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HAMA blocking reagent |
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| 85R-1026 | Fitzgerald | 50 mg | EUR 192 |
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Description: HAMA blocking reagent for use in immunoassays |
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Specimen Preservation Reagent |
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| DA0970 | Daan Gene | 100 test/kit | Ask for price |
Bradford Dye Reagent |
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| 0209R | AthenaES | 100 ml | EUR 131 |
BODIPY-Acetylene Reagent |
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| 2594-1 | Biovision | EUR 207 | |
BODIPY-Acetylene Reagent |
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| 2594-5 | Biovision | EUR 675 | |
ExFect2000 Transfection Reagent |
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| T202-01 | Vazyme | 0.5 ml | EUR 227 |
ExFect2000 Transfection Reagent |
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| T202-02 | Vazyme | 1 ml | EUR 316 |
ExFect2000 Transfection Reagent |
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| T202-03 | Vazyme | 5 ml | EUR 1052 |
Lung Lysate |
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| 1402 | ProSci | 0.1 mg | EUR 191 |
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Description: Lung tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Brain Lysate |
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| 1403 | ProSci | 0.1 mg | EUR 191 |
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Description: Brain tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Liver Lysate |
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| 1404 | ProSci | 0.1 mg | EUR 191 |
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Description: Liver tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Kidney Lysate |
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| 1405 | ProSci | 0.1 mg | EUR 191 |
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Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Spleen Lysate |
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| 1406 | ProSci | 0.1 mg | EUR 191 |
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Description: Spleen tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Thymus Lysate |
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| 1409 | ProSci | 0.1 mg | EUR 191 |
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Description: Thymus tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Bladder Lysate |
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| 1410 | ProSci | 0.1 mg | EUR 191 |
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Description: Bladder tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Colon Lysate |
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| 1411 | ProSci | 0.1 mg | EUR 191 |
|
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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RBDv1 contained a local viral sign peptide, RBDv2 -human tPA sign peptide. We transfected a CHO DG44 cell line, chosen stably transfected cells, and carried out just a few rounds of methotrexate-driven amplification of the genetic cassette within the genome. For the RBDv2 variant, a high-yield clonal producer cell line was obtained. Purified proteins had been analyzed by polyacrylamide gel electrophoresis in lowering and non-reducing situations and gel filtration; for RBDv2 protein, the monomeric type content material exceeded 90% for a number of sequence. Deglycosylation with PNGase F and mass spectrometry confirmed the presence of N-glycosylation.