Preclinical safety evaluation of a recombinant plasmid vector encoding mature human neutrophil peptide-1 by repeated local administrations in nonhuman primates
blocking peptide / EIA / electrophoresis / extract / Percp / plex / profile / Pure / Purification / Rabbit / Rhesus

Preclinical safety evaluation of a recombinant plasmid vector encoding mature human neutrophil peptide-1 by repeated local administrations in nonhuman primates

- August 24, 2021 | August 24, 2021 - Ajan

In our earlier research, a novel gene remedy method was developed based mostly on a plasmid vector pSecTag2B through which recombinant HNP1 gene was regulated below a cytomegalovirus promoter to encode a mature HNP1 type. We confirmed for the primary time in numerous tumor fashions together with human most cancers xenografts that overexpression of HNP1 within the tumor milieu by intratumoral pSecTag-HNP1 (pHNP1) administration effectively attenuated in vivo tumor development, mediated host immune responses to tumors, and produced a synergistic impact when mixed with chemotherapeutics.

In present research, a preclinical security investigation of HNP1 gene remedy was carried out in non-human primates. Eleven cynomolgus monkeys had been divided into three teams of three to four animals every and obtained both repeated s.c. injections of pHNP1/cationic liposome complexes at low (0.625 mg/kg) or excessive (2.5 mg/kg) dose or glucose as management. Important HNP1 in vivo accumulation was detected after consecutive administrations. All primates reached the top of the research with good physique situations. Injection web site irritation was the one apparent poisonous response throughout commentary interval.

As well as, elevation of monocyte/macrophage and neutrophil in addition to decline of lymphocyte had been detected within the peripheral blood of pHNP1-treated primates. These alterations had been partially alleviated on the finish of commentary interval. In addition to, dose-related histopathological adjustments of the immune organs had been noticed at necropsy, together with a minimal thymic lymphocyte lower and a minimal-to-mild lymph node erythrocyte enhance, however which can’t be excluded from HNP1 induced immune reactions. Collectively, these information help future medical research of pHNP1-based native gene supply in tumor sufferers.

The downregulation of endogenous dhfr improved the effectivity of EBNA-1 amplification, as evidenced by a comparability with the amplification extent in cells missing shRNA expression on the similar MTX focus. The EBNA-1 expression ranges from the EBNA-1-amplified clones chosen on this research had been larger than these obtained from EBNA-1-amplified clones that had been generated utilizing the standard amplification in our earlier research. According to earlier research, EBNA-1 amplification improved the manufacturing of the Fc-fusion protein by a selected protein productiveness (qp)-enhancing impact, slightly than by bettering cell development or transfection effectivity.

Joint common modular plasmids (JUMP): a versatile vector platform for artificial biology

Technology of latest DNA constructs is an important course of in trendy life science and biotechnology. Modular cloning techniques based mostly on Golden Gate cloning, utilizing Kind IIS restriction endonucleases, permit meeting of complicated multipart constructs from reusable fundamental DNA components in a fast, dependable and automation-friendly manner. Many such toolkits can be found, with various levels of compatibility, most of that are aimed toward particular host organisms. Right here, we current a vector design which permits easy vector modification by utilizing modular cloning to assemble and add new capabilities in secondary websites flanking the primary insertion web site (used for typical modular cloning).

Preclinical safety evaluation of a recombinant plasmid vector encoding mature human neutrophil peptide-1 by repeated local administrations in nonhuman primates

Meeting in all websites is suitable with the PhytoBricks normal, and vectors are suitable with the Commonplace European Vector Structure (SEVA) in addition to BioBricks. We exhibit that this facilitates the development of vectors with tailor-made capabilities and simplifies the workflow for producing libraries of constructs with frequent components. We have now made out there a set of vectors with 10 totally different microbial replication origins, various in copy quantity and host vary, and permitting chromosomal integration, in addition to a choice of generally used fundamental components.

This design expands the vary of hosts which might be simply modified by modular cloning and acts as a toolkit which can be utilized to facilitate the technology of latest toolkits with particular capabilities required for focusing on additional hosts. The antigen produced by the described approach is appropriate for serological exams and subunit vaccine research. We developed a easy purification scheme that constantly yielded as much as 30 mg of RBD protein per liter of the easy shake flask cell tradition.

Excessive-level expression of the monomeric SARS-CoV-2 S protein RBD 320-537 in stably transfected CHO cells by the EEF1A1-based plasmid vector

The spike (S) protein is likely one of the three proteins forming the coronaviruses’ viral envelope. The S protein of the Extreme Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial construction much like the S proteins of different mammalian coronaviruses, aside from a singular receptor-binding area (RBD), which is a big inducer of host immune response. Recombinant SARS-CoV-2 RBD is broadly used as a extremely particular minimal antigen for serological exams.

Right publicity of antigenic determinants has a big affect on the accuracy of such tests-the antigen must be appropriately folded, comprise no probably antigenic non-vertebrate glycans, and, ideally, ought to have a glycosylation sample much like the native S protein. Based mostly on the beforehand developed p1.1 vector, containing the regulatory sequences of the Eukaryotic translation elongation issue 1 alpha gene (EEF1A1) from Chinese language hamster, we created two expression constructs encoding SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine tags.

BMP2 Over-expression Lysate Product
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BMP10 (NM_014482) Human Over-expression Lysate
LS027302 100ug
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Description: Transient overexpression lysate of bone morphogenetic protein 10 (BMP10)

BMP15 (NM_005448) Human Over-expression Lysate
LS009210 100ug
EUR 628
Description: Transient overexpression lysate of bone morphogenetic protein 15 (BMP15)

BMP1 (NM_001199) Human Over-expression Lysate
LS000649 100ug
EUR 960
Description: Transient overexpression lysate of bone morphogenetic protein 1 (BMP1), transcript variant BMP1-1

BMP2 (NM_001200) Human Over-expression Lysate
LS000650 100ug
EUR 628
Description: Transient overexpression lysate of bone morphogenetic protein 2 (BMP2)

BMP3 (NM_001201) Human Over-expression Lysate
LS000651 100ug
EUR 960
Description: Transient overexpression lysate of bone morphogenetic protein 3 (BMP3)

BMP5 (NM_021073) Human Over-expression Lysate
LS000653 100ug
EUR 628
Description: Transient overexpression lysate of bone morphogenetic protein 5 (BMP5)

BMP6 (NM_001718) Human Over-expression Lysate
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Description: Transient overexpression lysate of bone morphogenetic protein 6 (BMP6)

BMP7 (NM_001719) Human Over-expression Lysate
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EUR 628
Description: Transient overexpression lysate of bone morphogenetic protein 7 (BMP7)

h BMP2 Expression, Concentrated Lentivirus
LVP481-PBS 1x10^8 IFU/ml x 200ul
EUR 455
Description: Pre-made express human target: BMP2 gene, carrying RFP-Blasticidin dual selection.

BMP2K (NM_198892) Human Over-expression Lysate
LS055589 100ug
EUR 960
Description: Transient overexpression lysate of BMP2 inducible kinase (BMP2K), transcript variant 1

BMP8A (NM_181809) Human Over-expression Lysate
LS353500 100ug
EUR 628
Description: Transient overexpression lysate of bone morphogenetic protein 8a (BMP8A)

BMP8B (NM_001720) Human Over-expression Lysate
LS000656 100ug
EUR 628
Description: Transient overexpression lysate of bone morphogenetic protein 8b (BMP8B)

BMPR2 Over-expression Lysate Product
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BMP9 (GDF2) (NM_016204) Human Over-expression Lysate
LS002658 100ug
EUR 628
Description: Transient overexpression lysate of growth differentiation factor 2 (GDF2)

BMPR2 (NM_001204) Human Over-expression Lysate
LS000659 100ug
EUR 628
Description: Transient overexpression lysate of bone morphogenetic protein receptor, type II (serine/threonine kinase) (BMPR2)

BMPR1A (NM_004329) Human Over-expression Lysate
LS000657 100ug
EUR 628
Description: Transient overexpression lysate of bone morphogenetic protein receptor, type IA (BMPR1A)

BMPR1B (NM_001203) Human Over-expression Lysate
LS000658 100ug
EUR 628
Description: Transient overexpression lysate of bone morphogenetic protein receptor, type IB (BMPR1B)

Mouse BMP4 HEK293 Overexpression Lysate
MBS8116605-03mg 0.3mg
EUR 280

Mouse BMP4 HEK293 Overexpression Lysate
MBS8116605-5x03mg 5x0.3mg
EUR 1205

Protein expression
CRO-4-Ind each Ask for price

EP Reagent Biuret Reagent - 100ML
1011601 1L
EUR 59.4

RFP expression Adenovirus, in vivo ready
AVP001-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made RFP expression adenovirus, provided in PBS solution.

CFP expression Adenovirus, in vivo ready
AVP002-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made CFP expression adenovirus, provided in PBS solution.

GFP expression Adenovirus, in vivo ready
AVP011-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made GFP expression adenovirus, provided in PBS solution.

YFP expression Adenovirus, in vivo ready
AVP012-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made YFP expression adenovirus, provided in PBS solution.

BFP expression Adenovirus, in vivo ready
AVP017-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made BFP expression adenovirus, provided in PBS solution.

YLEX Expression Kit
FYY201-1KT 1 kit Ask for price

PEROXIDE BLOCKING REAGENT Reagent
GWB-Q00308 50 ml Ask for price

EP Reagent Iodoplatinate Reagent - 200ML
1046300 200ML
EUR 502.2

EP Reagent Molybdovanadic Reagent - 100ML
1056700 100ML
EUR 59.4

h OCT4 expression Adenovirus, in vivo ready
AVP013-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made adenovirus express human stem cell factor, OCT4, provided in PBS solution for in vivo application.

h SOX2 expression Adenovirus, in vivo ready
AVP014-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made adenovirus express human stem cell factor, SOX2, provided in PBS solution for in vivo application.

293 Expression MAX-1
EXP-711-11 1ml
EUR 214
Description: 293 Expression MAX-1 is expressed from - -.

EP Reagent Methoxyphenylacetic Reagent - 100ML
1053601 100ML
EUR 490.05

h LIN28 expression Adenovirus, in vivo ready
AVP015-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made adenovirus express human stem cell factor, LIN28, provided in PBS solution for in vivo application.

CNGA2 Expression vector
60642 20 µg
EUR 900
Description: The CNGA2 expression vector is designed to express human cyclic nucleotide-gated cation channel 2 (CNGA2) in mammalian cells.

CNGA2 Expression vector
GWB-PSFA61 20ug Ask for price

LEXSYcon2.1 Expression Kit
EGE-1310ble 1Kit
EUR 1296.1
Description: for constitutive cytosolic or secretory protein expression

LEXSYcon2.1 Expression Kit
EGE-1310bsd 1Kit
EUR 1296.1
Description: for constitutive cytosolic or secretory protein expression

LEXSYcon2.1 Expression Kit
EGE-1310hyg 1Kit
EUR 1296.1
Description: for constitutive cytosolic or secretory protein expression

LEXSYcon2.1 Expression Kit
EGE-1310neo 1Kit
EUR 1296.1
Description: for constitutive cytosolic or secretory protein expression

LEXSYcon2.1 Expression Kit
EGE-1310pac 1Kit
EUR 1296.1
Description: for constitutive cytosolic or secretory protein expression

LEXSYcon2.1 Expression Kit
EGE-1310sat 1Kit
EUR 1296.1
Description: for constitutive cytosolic or secretory protein expression

pYLEX1 - Expression Vector
FYY203-5MG 5mg Ask for price

EP Reagent Phosphomolybdotungstic Reagent - 100ML
1065000 100ML
EUR 226.8

Blunt E1 Expression Kit
abx098098-10rxns 10 rxns
EUR 560.4

Blunt E2 Expression Kit
abx098873-10rxns 10 rxns
EUR 560.4

Blunt E1 Expression Kit
abx098098-100l 100 µl
EUR 712.5

Blunt E1 Expression Kit
abx098098-1ml 1 ml Ask for price

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abx098098-200l 200 µl Ask for price

Blunt E2 Expression Kit
abx098873-100l 100 µl
EUR 712.5

Blunt E2 Expression Kit
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Blunt E2 Expression Kit
abx098873-200l 200 µl Ask for price

RFP expression Adenovirus
AVP001 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made RFP expression adenovirus, provided in DMEM medium.

CFP expression Adenovirus
AVP002 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made CFP expression adenovirus, provided in DMEM medium.

GFP expression Adenovirus
AVP011 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made GFP expression adenovirus, provided in DMEM medium.

YFP expression Adenovirus
AVP012 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made YFP expression adenovirus, provided in DMEM medium.

BFP expression Adenovirus
AVP017 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made BFP expression adenovirus, provided in DMEM medium.

AVIDIN/BIOTIN BLOCKING REAGENT Reagent
GWB-Q00289 30 ml Ask for price

Gordon-McLeod Reagent (Oxidase reagent)
R026-100ML 1 unit
EUR 7.76
Description: Gordon-McLeod Reagent (Oxidase reagent)

LEXSinduce3 Expression Kit
EGE-1410ble 1Kit
EUR 3037.6
Description: for inducible cytosolic or secretory protein expression

LEXSinduce3 Expression Kit
EGE-1410blecherry 1Kit
EUR 3037.6
Description: for inducible cytosolic or secretory protein expression

LEXSinduce3 Expression Kit
EGE-1410neo 1Kit
EUR 3037.6
Description: for inducible cytosolic or secretory protein expression

LEXSinduce4 Expression Kit
EGE-1420blecherry 1Kit
EUR 3037.6
Description: for inducible cytosolic or secretory protein expression

Oxidase Reagent (Gordon-Macleod Reagent)
76207 100 ml
EUR 3.38
Description: Part B

Rac1 Expression Vector Set
STA-454 1 kit
EUR 1765.2
Description: Rac1 Expression Vector Set contains 3 vectors: Rac wild type, T17N dominant negative mutant, and G12V constitutively active mutant.

RhoA Expression Vector Set
STA-456 1 kit
EUR 1765.2
Description: RhoA Expression Vector Set contains 3 vectors: RhoA wild type, T19N dominant negative mutant, and G14V constitutively active mutant.

NEU3 Over-expression Lysate
GWB-DL2468 0.1 mg Ask for price

EP Reagent Sulfomolybdic Reagent R3 - 1L
1086500 1L
EUR 398.25

TRIpure Reagent (same as Trizol Reagent)
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Cdc42 Expression Vector Set
STA-455 1 kit
EUR 1765.2
Description: Cdc42 Expression Vector Set contains 3 vectors: Cdc42 wild type, T17N dominant negative mutant, and Q61L constitutively active mutant.

OPN expression inhibitor 1
T62820-10mg 10mg Ask for price
Description: OPN expression inhibitor 1

OPN expression inhibitor 1
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Description: OPN expression inhibitor 1

OPN expression inhibitor 1
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Description: OPN expression inhibitor 1

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Description: OPN expression inhibitor 1

OPN expression inhibitor 1
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Description: OPN expression inhibitor 1

OPN expression inhibitor 1
HY-146064 10 mg
EUR 2272.76
Description: OPN expression inhibitor 1 (Compound 11) is an osteopontin (OPN) expression inhibitor. OPN expression inhibitor 1 can be used for breast cancer research[1].

pAAV-MCS Expression Vector
VPK-410 10 µg
EUR 530

h IL6 Expression Lentivirus
LVP1144 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h CD4 Expression Lentivirus
LVP1152 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: CD4, containing a RFP-Blasticidin dual selection marker.

h IL4 Expression Lentivirus
LVP1161 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: IL4, containing a RFP-Blasticidin dual selection marker.

h IL7 Expression Lentivirus
LVP1162 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: IL7, containing a RFP-Blasticidin dual selection marker.

h KDR Expression Lentivirus
LVP1176 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: KDR, containing a RFP-Blasticidin dual selection marker.

h FAS Expression Lentivirus
LVP1181 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: FAS, containing a RFP-Blasticidin dual selection marker.

h ADA Expression Lentivirus
LVP923 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: ADA, containing a RFP-Blasticidin dual selection marker.

h FAP Expression Lentivirus
LVP1307 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: FAP, containing a RFP-Blasticidin dual selection marker.

h CD1 Expression Lentivirus
LVP1320 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: CD1, containing a RFP-Blasticidin dual selection marker.

m FAP Expression Lentivirus
LVP1326 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for mouse target: FAP, containing a RFP-Blasticidin dual selection marker.

h VIM Expression Lentivirus
LVP1357 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: VIM, containing a RFP-Blasticidin dual selection marker.

h PXN Expression Lentivirus
LVP1358 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: PXN, containing a RFP-Blasticidin dual selection marker.

m CD8 Expression Lentivirus
LVP1363 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for mouse target: CD8, containing a RFP-Blasticidin dual selection marker.

h TPO Expression Lentivirus
LVP1371 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: TPO, containing a RFP-Blasticidin dual selection marker.

h WAS Expression Lentivirus
LVP1429 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: WAS, containing a RFP-Blasticidin dual selection marker.

m PGR Expression Lentivirus
LVP1455 1x10^7 IFU/ml x 200ul
EUR 346.5
Description: Pre-made over-expression lentivirus for mouse target: PGR containing a RFP-Blasticidin dual selection marker.

h MAG Expression Lentivirus
LVP1485 1x10^7 IFU/ml x 200ul
EUR 346.5
Description: Pre-made over-expression lentivirus for human target: MAG, containing a RFP-Blasticidin dual selection marker.

h FST Expression Lentivirus
LVP1518 1x10^8 IFU/ml x 200ul
EUR 455
Description: Pre-made lentivirus express human FST gene, containing a RFP-Blasticidin dual selection marker.

pAAV-MCS Expression Vector
MBS169528-001mg 0.01mg
EUR 695

pAAV-MCS Expression Vector
MBS169528-5x001mg 5x0.01mg
EUR 2955

Total RNA Isolation Reagent (Reagent Based)
MBS402052-100mL 100mL
EUR 235

Total RNA Isolation Reagent (Reagent Based)
MBS402052-4Reactions 4Reactions
EUR 165

FlashBAC - Baculovirus Expression System (96 reactions)
GWB-6C34E3 Kit Ask for price

FlashBAC - Baculovirus Expression System (5 reactions)
GWB-7676BB Kit Ask for price

FlashBAC - Baculovirus Expression System(24 reactions)
GWB-EB1FF8 Kit Ask for price

h OCT4 expression Adenovirus
AVP013 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made adenovirus express human stem cell factor, OCT4, provided in DMEM medium.

h SOX2 expression Adenovirus
AVP014 1x109 IFU/ml x 50ul
EUR 418.8
Description: pre-made adenovirus express human stem cell factor, SOX2, provided in DMEM medium.

h EGFR Expression Lentivirus
LVP1142 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: EGFR, containing a RFP-Blasticidin dual selection marker.

h NRP1 Expression Lentivirus
LVP1145 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h MUC1 Expression Lentivirus
LVP1148 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h CD8a Expression Lentivirus
LVP1151 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: CD8a, containing a RFP-Blasticidin dual selection marker.

h PIGF Expression Lentivirus
LVP1154 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: PIGF, containing a RFP-Blasticidin dual selection marker.

h BRAF Expression Lentivirus
LVP1156 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: BRAF, containing a RFP-Blasticidin dual selection marker.

h ROS1 Expression Lentivirus
LVP1158 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: ROS1, containing a RFP-Blasticidin dual selection marker.

h CD3E Expression Lentivirus
LVP1163 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: IL7, containing a RFP-Blasticidin dual selection marker.

h KLK3 Expression Lentivirus
LVP1171 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: KLK3, containing a RFP-Blasticidin dual selection marker.

h NEK6 Expression Lentivirus
LVP1182 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: NEK6, containing a RFP-Blasticidin dual selection marker.

h NEK7 Expression Lentivirus
LVP1183 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: NEK7, containing a RFP-Blasticidin dual selection marker.

h CDR1 Expression Lentivirus
LVP917 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: CDR1, containing a RFP-Blasticidin dual selection marker.

h CD47 Expression Lentivirus
LVP918 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: CD47, containing a RFP-Blasticidin dual selection marker.

h ESR1 Expression Lentivirus
LVP924 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: ESR1, containing a RFP-Blasticidin dual selection marker.

r Syn1 Expression Lentivirus
LVP925 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for rat target: Syn1, containing a RFP-Blasticidin dual selection marker.

h NME4 Expression Lentivirus
LVP932 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: NME4, containing a RFP-Blasticidin dual selection marker.

h STM2 Expression Lentivirus
LVP1210 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Pre-made over-expression lentivirus for human target: STM2, containing a RFP-Blasticidin dual selection marker.

h CBX7 Expression Lentivirus
LVP1308 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: CBX7, containing a RFP-Blasticidin dual selection marker.

h ACE2 Expression Lentivirus
LVP1310 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: ACE2, containing a RFP-Blasticidin dual selection marker.

h Rab7 Expression Lentivirus
LVP1315 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: Rab7, containing a RFP-Blasticidin dual selection marker.

h Rab5 Expression Lentivirus
LVP1316 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: Rab5, containing a RFP-Blasticidin dual selection marker.

m ASPA Expression Lentivirus
LVP1317 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for mouse target: ASPA, containing a RFP-Blasticidin dual selection marker.

h CD44 Expression Lentivirus
LVP1318 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: CD44, containing a RFP-Blasticidin dual selection marker.

h MFN2 Expression Lentivirus
LVP1327 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: MFN2, containing a RFP-Blasticidin dual selection marker.

h ANO1 Expression Lentivirus
LVP1344 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: ANO1 containing a RFP-Blasticidin dual selection marker.

h FGL2 Expression Lentivirus
LVP1346 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: FGL2, containing a RFP-Blasticidin dual selection marker.

h CCR2 Expression Lentivirus
LVP1354 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: CCR2, containing a RFP-Blasticidin dual selection marker.

h NEFL Expression Lentivirus
LVP1360 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: NEFL, containing a RFP-Blasticidin dual selection marker.

h NOS2 Expression Lentivirus
LVP1367 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: NOS2, containing a RFP-Blasticidin dual selection marker.

h BMI1 Expression Lentivirus
LVP1372 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: BMI1, containing a RFP-Blasticidin dual selection marker.

h PSCA Expression Lentivirus
LVP1374 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: PSCA, containing a RFP-Blasticidin dual selection marker.

h POLQ Expression Lentivirus
LVP1421 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: POLQ containing a RFP-Blasticidin dual selection marker.

h ARSA Expression Lentivirus
LVP1425 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: ARSA, containing a RFP-Blasticidin dual selection marker.

h IDUA Expression Lentivirus
LVP1426 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: IDUA, containing a RFP-Blasticidin dual selection marker.

h SGSH Expression Lentivirus
LVP1427 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: SGSH, containing a RFP-Blasticidin dual selection marker.

h CYBB Expression Lentivirus
LVP1430 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: CYBB, containing a RFP-Blasticidin dual selection marker.

h CAV1 Expression Lentivirus
LVP1431 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: CAV1, containing a RFP-Blasticidin dual selection marker.

m INS2 Expression Lentivirus
LVP1456 1x10^7 IFU/ml x 200ul
EUR 346.5
Description: Pre-made over-expression lentivirus for mouse target: INS2 containing a RFP-Blasticidin dual selection marker.

h ROR1 Expression Lentivirus
LVP1458 1x10^7 IFU/ml x 200ul
EUR 346.5
Description: Pre-made over-expression lentivirus for human target: ROR1, containing a RFP-Blasticidin dual selection marker.

h DGKA Expression Lentivirus
LVP1468 1x10^7 IFU/ml x 200ul
EUR 346.5
Description: Pre-made over-expression lentivirus for human target: DGKA, containing a RFP-Blasticidin dual selection marker.

h REST_HA Expression Lentivirus
LVP1475 1x10^7 IFU/ml x 200ul
EUR 346.5
Description: Pre-made over-expression lentivirus for human target: REST, containing a RFP-Blasticidin dual selection marker.

h UBR7 Expression Lentivirus
LVP1483 1x10^7 IFU/ml x 200ul
EUR 346.5
Description: Pre-made over-expression lentivirus for human target: UBR7, containing a RFP-Blasticidin dual selection marker.

h MMP1 Expression Lentivirus
LVP1516 1x10^8 IFU/ml x 200ul
EUR 455
Description: Pre-made lentivirus express human MMP1 gene, containing a RFP-Blasticidin dual selection marker.

h BDNF Expression Lentivirus
LVP1520 1x10^8 IFU/ml x 200ul
EUR 455
Description: Pre-made lentivirus express human BDNF gene, containing a RFP-Blasticidin dual selection marker.

m CCL5 Expression Lentivirus
LVP1619 1x10^8 IFU/ml x 200ul
EUR 455
Description: Pre-made lentivirus express mouse CCL5 gene, containing a RFP-Blasticidin dual selection marker.

Genomic DNA Isolation Reagent (Reagent Based)
MBS402050-100mL 100mL
EUR 255

Genomic DNA Isolation Reagent (Reagent Based)
MBS402050-4Reactions 4Reactions
EUR 185

h LIN28 expression Adenovirus
AVP015 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made adenovirus express human stem cell factor, LIN28, provided in DMEM medium.

pscAAV-MCS Expression Vector
VPK-430 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

h HDAC1 Expression Lentivirus
LVP1143 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h VEGFB Expression Lentivirus
LVP1146 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h PARP1 Expression Lentivirus
LVP1149 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h IL3RA Expression Lentivirus
LVP1150 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: IL3RA, containing a RFP-Blasticidin dual selection marker.

h PTCH1 Expression Lentivirus
LVP1155 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: PTCH1, containing a RFP-Blasticidin dual selection marker.

RBDv1 contained a local viral sign peptide, RBDv2 -human tPA sign peptide. We transfected a CHO DG44 cell line, chosen stably transfected cells, and carried out just a few rounds of methotrexate-driven amplification of the genetic cassette within the genome. For the RBDv2 variant, a high-yield clonal producer cell line was obtained. Purified proteins had been analyzed by polyacrylamide gel electrophoresis in lowering and non-reducing situations and gel filtration; for RBDv2 protein, the monomeric type content material exceeded 90% for a number of sequence. Deglycosylation with PNGase F and mass spectrometry confirmed the presence of N-glycosylation.

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