In our earlier research, a novel gene remedy method was developed based mostly on a plasmid vector pSecTag2B through which recombinant HNP1 gene was regulated below a cytomegalovirus promoter to encode a mature HNP1 type. We confirmed for the primary time in numerous tumor fashions together with human most cancers xenografts that overexpression of HNP1 within the tumor milieu by intratumoral pSecTag-HNP1 (pHNP1) administration effectively attenuated in vivo tumor development, mediated host immune responses to tumors, and produced a synergistic impact when mixed with chemotherapeutics.
In present research, a preclinical security investigation of HNP1 gene remedy was carried out in non-human primates. Eleven cynomolgus monkeys had been divided into three teams of three to four animals every and obtained both repeated s.c. injections of pHNP1/cationic liposome complexes at low (0.625 mg/kg) or excessive (2.5 mg/kg) dose or glucose as management. Important HNP1 in vivo accumulation was detected after consecutive administrations. All primates reached the top of the research with good physique situations. Injection web site irritation was the one apparent poisonous response throughout commentary interval.
As well as, elevation of monocyte/macrophage and neutrophil in addition to decline of lymphocyte had been detected within the peripheral blood of pHNP1-treated primates. These alterations had been partially alleviated on the finish of commentary interval. In addition to, dose-related histopathological adjustments of the immune organs had been noticed at necropsy, together with a minimal thymic lymphocyte lower and a minimal-to-mild lymph node erythrocyte enhance, however which can’t be excluded from HNP1 induced immune reactions. Collectively, these information help future medical research of pHNP1-based native gene supply in tumor sufferers.
The downregulation of endogenous dhfr improved the effectivity of EBNA-1 amplification, as evidenced by a comparability with the amplification extent in cells missing shRNA expression on the similar MTX focus. The EBNA-1 expression ranges from the EBNA-1-amplified clones chosen on this research had been larger than these obtained from EBNA-1-amplified clones that had been generated utilizing the standard amplification in our earlier research. According to earlier research, EBNA-1 amplification improved the manufacturing of the Fc-fusion protein by a selected protein productiveness (qp)-enhancing impact, slightly than by bettering cell development or transfection effectivity.
Joint common modular plasmids (JUMP): a versatile vector platform for artificial biology
Technology of latest DNA constructs is an important course of in trendy life science and biotechnology. Modular cloning techniques based mostly on Golden Gate cloning, utilizing Kind IIS restriction endonucleases, permit meeting of complicated multipart constructs from reusable fundamental DNA components in a fast, dependable and automation-friendly manner. Many such toolkits can be found, with various levels of compatibility, most of that are aimed toward particular host organisms. Right here, we current a vector design which permits easy vector modification by utilizing modular cloning to assemble and add new capabilities in secondary websites flanking the primary insertion web site (used for typical modular cloning).
Meeting in all websites is suitable with the PhytoBricks normal, and vectors are suitable with the Commonplace European Vector Structure (SEVA) in addition to BioBricks. We exhibit that this facilitates the development of vectors with tailor-made capabilities and simplifies the workflow for producing libraries of constructs with frequent components. We have now made out there a set of vectors with 10 totally different microbial replication origins, various in copy quantity and host vary, and permitting chromosomal integration, in addition to a choice of generally used fundamental components.
This design expands the vary of hosts which might be simply modified by modular cloning and acts as a toolkit which can be utilized to facilitate the technology of latest toolkits with particular capabilities required for focusing on additional hosts. The antigen produced by the described approach is appropriate for serological exams and subunit vaccine research. We developed a easy purification scheme that constantly yielded as much as 30 mg of RBD protein per liter of the easy shake flask cell tradition.
Excessive-level expression of the monomeric SARS-CoV-2 S protein RBD 320-537 in stably transfected CHO cells by the EEF1A1-based plasmid vector
The spike (S) protein is likely one of the three proteins forming the coronaviruses’ viral envelope. The S protein of the Extreme Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial construction much like the S proteins of different mammalian coronaviruses, aside from a singular receptor-binding area (RBD), which is a big inducer of host immune response. Recombinant SARS-CoV-2 RBD is broadly used as a extremely particular minimal antigen for serological exams.
Right publicity of antigenic determinants has a big affect on the accuracy of such tests-the antigen must be appropriately folded, comprise no probably antigenic non-vertebrate glycans, and, ideally, ought to have a glycosylation sample much like the native S protein. Based mostly on the beforehand developed p1.1 vector, containing the regulatory sequences of the Eukaryotic translation elongation issue 1 alpha gene (EEF1A1) from Chinese language hamster, we created two expression constructs encoding SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine tags.
Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-Hu-48T | DL Develop | 48T | EUR 510 |
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Description: A sandwich quantitative ELISA assay kit for detection of Human Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-Hu-96T | DL Develop | 96T | EUR 657.6 |
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Description: A sandwich quantitative ELISA assay kit for detection of Human Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-Mu-48T | DL Develop | 48T | EUR 522 |
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Description: A sandwich quantitative ELISA assay kit for detection of Mouse Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-Mu-96T | DL Develop | 96T | EUR 673.2 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-p-48T | DL Develop | 48T | EUR 591.6 |
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Description: A sandwich quantitative ELISA assay kit for detection of Porcine Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-p-96T | DL Develop | 96T | EUR 769.2 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-Ra-48T | DL Develop | 48T | EUR 544.8 |
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Description: A sandwich quantitative ELISA assay kit for detection of Rat Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-Ra-96T | DL Develop | 96T | EUR 704.4 |
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Description: A sandwich quantitative ELISA assay kit for detection of Rat Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-Rb-48T | DL Develop | 48T | EUR 544.8 |
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Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-Rb-96T | DL Develop | 96T | EUR 704.4 |
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Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Bovine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-b-48Tests | Reddot Biotech | 48 Tests | EUR 592.8 |
Bovine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-b-96Tests | Reddot Biotech | 96 Tests | EUR 820.8 |
Canine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-c-48Tests | Reddot Biotech | 48 Tests | EUR 566.4 |
Canine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-c-96Tests | Reddot Biotech | 96 Tests | EUR 783.6 |
Chicken Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-Ch-48Tests | Reddot Biotech | 48 Tests | EUR 540 |
Chicken Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-Ch-96Tests | Reddot Biotech | 96 Tests | EUR 746.4 |
Goat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-g-48Tests | Reddot Biotech | 48 Tests | EUR 606 |
Goat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-g-96Tests | Reddot Biotech | 96 Tests | EUR 840 |
Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 501.6 |
Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 690 |
Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 514.8 |
Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 709.2 |
Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
|||
| RD-BMP4-p-48Tests | Reddot Biotech | 48 Tests | EUR 592.8 |
Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
|||
| RD-BMP4-p-96Tests | Reddot Biotech | 96 Tests | EUR 820.8 |
Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 540 |
Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 746.4 |
Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-Rb-48Tests | Reddot Biotech | 48 Tests | EUR 540 |
Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-Rb-96Tests | Reddot Biotech | 96 Tests | EUR 746.4 |
Bovine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
|||
| RDR-BMP4-b-48Tests | Reddot Biotech | 48 Tests | EUR 619.2 |
Bovine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-b-96Tests | Reddot Biotech | 96 Tests | EUR 859.2 |
Canine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-c-48Tests | Reddot Biotech | 48 Tests | EUR 591.6 |
Canine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
|||
| RDR-BMP4-c-96Tests | Reddot Biotech | 96 Tests | EUR 819.6 |
Chicken Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-Ch-48Tests | Reddot Biotech | 48 Tests | EUR 564 |
Chicken Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-Ch-96Tests | Reddot Biotech | 96 Tests | EUR 781.2 |
Goat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-g-48Tests | Reddot Biotech | 48 Tests | EUR 633.6 |
Goat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-g-96Tests | Reddot Biotech | 96 Tests | EUR 879.6 |
Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
|||
| RDR-BMP4-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 523.2 |
Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 721.2 |
Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
|||
| RDR-BMP4-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 536.4 |
Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
|||
| RDR-BMP4-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 741.6 |
Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
|||
| RDR-BMP4-p-48Tests | Reddot Biotech | 48 Tests | EUR 619.2 |
Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
|||
| RDR-BMP4-p-96Tests | Reddot Biotech | 96 Tests | EUR 859.2 |
Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 564 |
Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
|||
| RDR-BMP4-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 781.2 |
Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-Rb-48Tests | Reddot Biotech | 48 Tests | EUR 564 |
Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-Rb-96Tests | Reddot Biotech | 96 Tests | EUR 781.2 |
Visitor Over Spectacle Clear |
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| SAF1410 | Scientific Laboratory Supplies | EACH | EUR 4.56 |
EP Reagent Biuret Reagent |
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| 1011601 | Scientific Laboratory Supplies | 1L | EUR 42.18 |
EP Reagent Iodoplatinate Reagent |
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| 1046300 | Scientific Laboratory Supplies | 200ML | EUR 360.24 |
EP Reagent Methoxyphenylacetic Reagent |
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| 1053601 | Scientific Laboratory Supplies | 100ML | EUR 354.54 |
EP Reagent Molybdovanadic Reagent |
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| 1056700 | Scientific Laboratory Supplies | 100ML | EUR 42.18 |
EP Reagent Phosphomolybdotungstic Reagent |
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| 1065000 | Scientific Laboratory Supplies | 100ML | EUR 161.88 |
Automatic CO2 Change Over Unit |
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| INC6176 | Scientific Laboratory Supplies | EACH | EUR 1288.2 |
Forceps Dissecting Turn Over End |
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| D02129 | Scientific Laboratory Supplies | EACH | EUR 4.67 |
Gas Change Over Unit 30Psi |
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| GAS1000 | Scientific Laboratory Supplies | EACH | EUR 904.02 |
Gas Change Over Unit 60Psi |
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| GAS1002 | Scientific Laboratory Supplies | EACH | EUR 905.16 |
Gas Change Over Unit 100Psi |
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| GAS1004 | Scientific Laboratory Supplies | EACH | EUR 922.26 |
Bolle TG10 Safety Over Glasses |
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| SAF1106 | Scientific Laboratory Supplies | EACH | EUR 8.39 |
EP Reagent Sulfomolybdic Reagent R3 |
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| 1086500 | Scientific Laboratory Supplies | 1L | EUR 287.28 |
DURAN Over-Cap 45mm Black Phenolic |
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| BOT2596 | Scientific Laboratory Supplies | PK10 | EUR 25.08 |
BOP reagent |
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| 5-02141 | CHI Scientific | 25g | Ask for price |
BOP reagent |
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| 5-02142 | CHI Scientific | 100g | Ask for price |
Chymase reagent |
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| 30C-CP1129 | Fitzgerald | 5 units | EUR 2622 |
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Description: Purified native Human Chymase reagent |
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Traut's Reagent |
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| 2330-1000 | Biovision | EUR 418.8 | |
Traut's Reagent |
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| 2330-500 | Biovision | EUR 248.4 | |
MTS Reagent |
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| 2808-1000 | Biovision | EUR 1188 | |
MTS Reagent |
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| 2808-250 | Biovision | EUR 438 | |
MTT Reagent |
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| 2809-1G | Biovision | EUR 216 | |
MTT Reagent |
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| 2809-5G | Biovision | EUR 652.8 | |
BOP reagent |
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| A7015-100000 | ApexBio | 100 g | EUR 240 |
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Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis. |
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BOP reagent |
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| A7015-25000 | ApexBio | 25 g | EUR 135.6 |
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Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis. |
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Bradford reagent |
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| BDE641 | Bio Basic | 100ml | EUR 73.21 |
Bluing Reagent |
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| BRT030 | ScyTek Laboratories | 30 ml | EUR 72 |
Bluing Reagent |
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| BRT125 | ScyTek Laboratories | 125 ml | EUR 75.6 |
Bluing Reagent |
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| BRT3800 | ScyTek Laboratories | 1 Gal. | EUR 220.8 |
Bluing Reagent |
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| BRT500 | ScyTek Laboratories | 500 ml | EUR 91.2 |
Bluing Reagent |
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| BRT999 | ScyTek Laboratories | 1000 ml | EUR 105.6 |
Beaucage reagent |
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| HY-100951 | MedChemExpress | 10mM/1mL | EUR 151.2 |
Phosphate Reagent |
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| 106199 | Scientific Laboratory Supplies | PK100 | EUR 61.29 |
Chromium Reagent |
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| 1206699 | Scientific Laboratory Supplies | EACH | EUR 141.72 |
Ninhydrin Reagent |
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| MIC6746 | Scientific Laboratory Supplies | EACH | EUR 31.12 |
Nessler Reagent |
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| NESSR | Scientific Laboratory Supplies | 500ML | EUR 148.2 |
Thioacetamide Reagent |
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| THIOR01 | Scientific Laboratory Supplies | 100ML | EUR 78.66 |
BMP4 antibody |
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| 70R-12408 | Fitzgerald | 100 ug | EUR 483.6 |
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Description: Rabbit polyclonal BMP4 antibody |
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BMP4 antibody |
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| 70R-12409 | Fitzgerald | 100 ug | EUR 483.6 |
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Description: Rabbit polyclonal BMP4 antibody |
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BMP4 Antibody |
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| 43852-100ul | SAB | 100ul | EUR 302.4 |
BMP4 antibody |
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| 70R-16011 | Fitzgerald | 50 ul | EUR 522 |
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Description: Rabbit polyclonal BMP4 antibody |
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BMP4 antibody |
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| 38256-100ul | SAB | 100ul | EUR 302.4 |
BMP4 protein |
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| 30R-1255 | Fitzgerald | 100 ug | EUR 268.8 |
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Description: Purified recombinant Human BMP4 protein |
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BMP4 protein |
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| 30R-2651 | Fitzgerald | 10 ug | EUR 409.2 |
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Description: Purified recombinant Human BMP4 protein |
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BMP4 Antibody |
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| 35652-100ul | SAB | 100ul | EUR 302.4 |
BMP4 antibody |
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| 20R-2936 | Fitzgerald | 100 ul | EUR 471.6 |
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Description: Rabbit polyclonal BMP4 antibody |
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BMP4 protein |
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| 30R-AB029 | Fitzgerald | 5 ug | EUR 327.6 |
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Description: Purified recombinant Human BMP4 protein |
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BMP4 antibody |
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| 10R-B119a | Fitzgerald | 100 ug | EUR 950.4 |
|
Description: Mouse monoclonal BMP4 antibody |
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BMP4 antibody |
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| 10R-B119B | Fitzgerald | 500 ug | EUR 327.6 |
|
Description: Mouse monoclonal BMP4 antibody |
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BMP4 antibody |
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| 10R-2148 | Fitzgerald | 100 ul | EUR 483.6 |
|
Description: Mouse monoclonal BMP4 antibody |
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BMP4 antibody |
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| 10R-3460 | Fitzgerald | 100 ul | EUR 829.2 |
|
Description: Mouse monoclonal BMP4 antibody |
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BMP4 Antibody |
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| ABF5175 | Lifescience Market | 100 ug | EUR 525.6 |
BMP4 Antibody |
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| ABD6461 | Lifescience Market | 100 ug | EUR 525.6 |
BMP4 siRNA |
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| 20-abx909174 | Abbexa |
|
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BMP4 siRNA |
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| 20-abx909175 | Abbexa |
|
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BMP4 Antibody |
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| BF0696 | Affbiotech | 200ul | EUR 540 |
BMP4 Antibody |
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| 1-CSB-PA763132 | Cusabio |
|
|
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Description: A polyclonal antibody against BMP4. Recognizes BMP4 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:25-1:100 |
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BMP4 Antibody |
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| 1-CSB-PA05799A0Rb | Cusabio |
|
|
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Description: A polyclonal antibody against BMP4. Recognizes BMP4 from Human, Mouse, Rat, Zebrafish. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:100-1:1000 |
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BMP4 Antibody |
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| DF6461 | Affbiotech | 200ul | EUR 420 |
BMP4 Antibody |
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| AF5175 | Affbiotech | 200ul | EUR 420 |
BMP4 Antibody |
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| 1-CSB-PA212919 | Cusabio |
|
|
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Description: A polyclonal antibody against BMP4. Recognizes BMP4 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:30-1:150 |
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BMP4 Antibody |
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| 1-CSB-PA002740GA01HU | Cusabio |
|
|
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Description: A polyclonal antibody against BMP4. Recognizes BMP4 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB |
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anti-BMP4 |
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| LF-PA0093 | Abfrontier | 100 ul | EUR 400.8 |
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Description: Rabbit polyclonal to BMP4 |
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pENTR223- BMP4 |
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| PVT10009 | Lifescience Market | 2 ug | EUR 319.2 |
pCDNA3.1- BMP4 |
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| PVT10207 | Lifescience Market | 2 ug | EUR 361.2 |
BMP4 Antibody |
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| R31950 | NSJ Bioreagents | 100 ug | EUR 419 |
BMP4 Antibody |
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| R36461-100UG | NSJ Bioreagents | 100 ug | EUR 399 |
BMP4 Antibody |
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| F45385-0.08ML | NSJ Bioreagents | 0.08 ml | EUR 165 |
BMP4 Antibody |
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| F45385-0.4ML | NSJ Bioreagents | 0.4 ml | EUR 379 |
HEK-293T Telomerase Over-Expressing Cell Pellet |
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| abx069991-1Pellet | Abbexa | 1 Pellet | EUR 477.6 |
Visitor Eye Shield Over Specs - Portwest PW30 |
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| SAF1021 | Scientific Laboratory Supplies | EACH | EUR 3.42 |
Esophagus Lysate |
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| 1365 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: Esophagus tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Ileum Lysate |
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| 1367 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: Ileum tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Rectum Lysate |
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| 1373 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: Rectum tissue lysate was prepared by homogenization in homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Skin Lysate |
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| 1376 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: Skin tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Thyroid Lysate |
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| 1380 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: Thyroid tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Spleen Lysate |
|||
| 1406 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: Spleen tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Bladder Lysate |
|||
| 1410 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: Bladder tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Cerebellum Lysate |
|||
| 1412 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: Cerebellum tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Cerebrum Lysate |
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| 1413 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: Cerebrum tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Pancreas Lysate |
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| 1414 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Stomach Lysate |
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| 1415 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: Stomach tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Testis Lysate |
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| 1416 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: Testis tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Adrenal Lysate |
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| 1417 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: Adrenal tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Skin Lysate |
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| 1419 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: Skin tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Eye Lysate |
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| 1420 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: Eye tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Trachea Lysate |
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| 1422 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: Trachea tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Lung Lysate |
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| 1462 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: Lung tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Liver Lysate |
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| 1464 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: Liver tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Kidney Lysate |
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| 1465 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Spleen Lysate |
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| 1466 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: Spleen tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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L1210 Lysate |
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| 1284 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: L1210 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The L1210 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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C2C12 Lysate |
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| 1285 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: C2C12 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The C2C12 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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P815 Lysate |
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| 1286 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: P815 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The P815 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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EL4 Lysate |
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| 1287 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: EL4 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The EL4 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Lung Lysate |
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| 1302 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: Lung tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Spleen Lysate |
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| 1306 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: Spleen tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Placenta Lysate |
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| 1309 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: Placenta tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Melanoma Lysate |
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| 20-101 | ProSci | 0.1 mg | EUR 632.4 |
|
Description: Melanoma lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human melanoma tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the melanoma tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The melanoma tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Trachea Lysate |
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| 20-102 | ProSci | 0.1 mg | EUR 500.1 |
|
Description: Human trachea lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human trachea tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the trachea tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The trachea tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Jurkat Lysate |
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| 1205 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: Jurkat lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The Jurkat lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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MOLT4 Lysate |
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| 1206 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: MOLT4 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The MOLT4 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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HL60 Lysate |
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| 1209 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: HL60 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The HL60 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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T24 Lysate |
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| 1213 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: T24 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The T24 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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U937 Lysate |
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| 1215 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: U937 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The U937 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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MCF7 Lysate |
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| 1219 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: MCF7 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The MCF7 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Ramos Lysate |
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| 1225 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: Ramos lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The Ramos lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Kidney Lysate |
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| 21-104 | ProSci | 0.1 mg | EUR 342.6 |
|
Description: Bovine kidney tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The bovine kidney tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the kidney tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The kidney tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Liver Lysate |
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| 21-105 | ProSci | 0.1 mg | EUR 342.6 |
|
Description: Bovine liver tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The bovine liver tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the liver tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The liver tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Heart Lysate |
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| 21-115 | ProSci | 0.1 mg | EUR 342.6 |
|
Description: Guinea Pig heart tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The guinea pig heart tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the heart tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The heart tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Kidney Lysate |
|||
| 21-116 | ProSci | 0.1 mg | EUR 342.6 |
|
Description: Guinea Pig kidney tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The guinea pig kidney tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the kidney tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The kidney tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Adrenal Lysate |
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| 21-160 | ProSci | 0.1 mg | EUR 468.6 |
|
Description: Monkey (Cynomolgus) adrenal tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) adrenal tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the adrenal tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The adrenal tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Colon Lysate |
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| 21-179 | ProSci | 0.1 mg | EUR 342.6 |
|
Description: Monkey (Cynomolgus) colon tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) colon tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the colon tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The colon tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Gallbladder Lysate |
|||
| 21-188 | ProSci | 0.1 mg | EUR 468.6 |
|
Description: Monkey (Cynomolgus) gallbladder tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) gallbladder tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the gallbladder tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The gallbladder tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Kidney Lysate |
|||
| 21-190 | ProSci | 0.1 mg | EUR 342.6 |
|
Description: Monkey (Cynomolgus) kidney tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) kidney tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the kidney tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The kidney tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Lung Lysate |
|||
| 21-194 | ProSci | 0.1 mg | EUR 342.6 |
|
Description: Monkey (Cynomolgus) lung tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) lung tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the lung tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The lung tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Skin Lysate |
|||
| 21-204 | ProSci | 0.1 mg | EUR 468.6 |
|
Description: Monkey (Cynomolgus) skin tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) skin tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the skin tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The skin tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Spleen Lysate |
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| 21-209 | ProSci | 0.1 mg | EUR 342.6 |
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Description: Monkey (Cynomolgus) spleen tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) spleen tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the spleen tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The spleen tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Brain Lysate |
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| 21-272 | ProSci | 0.1 mg | EUR 342.6 |
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Description: Monkey (Rhesus) brain tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Rhesus) brain tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the brain tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The brain tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Colon Lysate |
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| 21-288 | ProSci | 0.1 mg | EUR 342.6 |
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Description: Monkey (Rhesus) colon tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Rhesus) colon tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the colon tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The colon tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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RBDv1 contained a local viral sign peptide, RBDv2 -human tPA sign peptide. We transfected a CHO DG44 cell line, chosen stably transfected cells, and carried out just a few rounds of methotrexate-driven amplification of the genetic cassette within the genome. For the RBDv2 variant, a high-yield clonal producer cell line was obtained. Purified proteins had been analyzed by polyacrylamide gel electrophoresis in lowering and non-reducing situations and gel filtration; for RBDv2 protein, the monomeric type content material exceeded 90% for a number of sequence. Deglycosylation with PNGase F and mass spectrometry confirmed the presence of N-glycosylation.