In our earlier research, a novel gene remedy method was developed based mostly on a plasmid vector pSecTag2B through which recombinant HNP1 gene was regulated below a cytomegalovirus promoter to encode a mature HNP1 type. We confirmed for the primary time in numerous tumor fashions together with human most cancers xenografts that overexpression of HNP1 within the tumor milieu by intratumoral pSecTag-HNP1 (pHNP1) administration effectively attenuated in vivo tumor development, mediated host immune responses to tumors, and produced a synergistic impact when mixed with chemotherapeutics.
In present research, a preclinical security investigation of HNP1 gene remedy was carried out in non-human primates. Eleven cynomolgus monkeys had been divided into three teams of three to four animals every and obtained both repeated s.c. injections of pHNP1/cationic liposome complexes at low (0.625 mg/kg) or excessive (2.5 mg/kg) dose or glucose as management. Important HNP1 in vivo accumulation was detected after consecutive administrations. All primates reached the top of the research with good physique situations. Injection web site irritation was the one apparent poisonous response throughout commentary interval.
As well as, elevation of monocyte/macrophage and neutrophil in addition to decline of lymphocyte had been detected within the peripheral blood of pHNP1-treated primates. These alterations had been partially alleviated on the finish of commentary interval. In addition to, dose-related histopathological adjustments of the immune organs had been noticed at necropsy, together with a minimal thymic lymphocyte lower and a minimal-to-mild lymph node erythrocyte enhance, however which can’t be excluded from HNP1 induced immune reactions. Collectively, these information help future medical research of pHNP1-based native gene supply in tumor sufferers.
The downregulation of endogenous dhfr improved the effectivity of EBNA-1 amplification, as evidenced by a comparability with the amplification extent in cells missing shRNA expression on the similar MTX focus. The EBNA-1 expression ranges from the EBNA-1-amplified clones chosen on this research had been larger than these obtained from EBNA-1-amplified clones that had been generated utilizing the standard amplification in our earlier research. According to earlier research, EBNA-1 amplification improved the manufacturing of the Fc-fusion protein by a selected protein productiveness (qp)-enhancing impact, slightly than by bettering cell development or transfection effectivity.
Joint common modular plasmids (JUMP): a versatile vector platform for artificial biology
Technology of latest DNA constructs is an important course of in trendy life science and biotechnology. Modular cloning techniques based mostly on Golden Gate cloning, utilizing Kind IIS restriction endonucleases, permit meeting of complicated multipart constructs from reusable fundamental DNA components in a fast, dependable and automation-friendly manner. Many such toolkits can be found, with various levels of compatibility, most of that are aimed toward particular host organisms. Right here, we current a vector design which permits easy vector modification by utilizing modular cloning to assemble and add new capabilities in secondary websites flanking the primary insertion web site (used for typical modular cloning).
Meeting in all websites is suitable with the PhytoBricks normal, and vectors are suitable with the Commonplace European Vector Structure (SEVA) in addition to BioBricks. We exhibit that this facilitates the development of vectors with tailor-made capabilities and simplifies the workflow for producing libraries of constructs with frequent components. We have now made out there a set of vectors with 10 totally different microbial replication origins, various in copy quantity and host vary, and permitting chromosomal integration, in addition to a choice of generally used fundamental components.
This design expands the vary of hosts which might be simply modified by modular cloning and acts as a toolkit which can be utilized to facilitate the technology of latest toolkits with particular capabilities required for focusing on additional hosts. The antigen produced by the described approach is appropriate for serological exams and subunit vaccine research. We developed a easy purification scheme that constantly yielded as much as 30 mg of RBD protein per liter of the easy shake flask cell tradition.
Excessive-level expression of the monomeric SARS-CoV-2 S protein RBD 320-537 in stably transfected CHO cells by the EEF1A1-based plasmid vector
The spike (S) protein is likely one of the three proteins forming the coronaviruses’ viral envelope. The S protein of the Extreme Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial construction much like the S proteins of different mammalian coronaviruses, aside from a singular receptor-binding area (RBD), which is a big inducer of host immune response. Recombinant SARS-CoV-2 RBD is broadly used as a extremely particular minimal antigen for serological exams.
Right publicity of antigenic determinants has a big affect on the accuracy of such tests-the antigen must be appropriately folded, comprise no probably antigenic non-vertebrate glycans, and, ideally, ought to have a glycosylation sample much like the native S protein. Based mostly on the beforehand developed p1.1 vector, containing the regulatory sequences of the Eukaryotic translation elongation issue 1 alpha gene (EEF1A1) from Chinese language hamster, we created two expression constructs encoding SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine tags.
BMP4 (NM_130851) Human Over-expression Lysate |
|||
| E45H13674-2 | EnoGene | each | EUR 395 |
BMP4 (NM_001202) Human Over-expression Lysate |
|||
| LS000652 | BosterBio | 100ug | EUR 628 |
|
Description: Transient overexpression lysate of bone morphogenetic protein 4 (BMP4), transcript variant 1 |
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BMP10 Over-expression Lysate Product |
|||
| GWB-C6E935 | GenWay Biotech | 0.1 mg | Ask for price |
h BMP2 Expression, Concentrated Lentivirus |
|||
| LVP481-PBS | GenTarget | 1x10^8 IFU/ml x 200ul | EUR 455 |
|
Description: Pre-made express human target: BMP2 gene, carrying RFP-Blasticidin dual selection. |
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BMP5 Over-expression Lysate Product |
|||
| GWB-88C35F | GenWay Biotech | 0.1 mg | Ask for price |
BMP3 Over-expression Lysate Product |
|||
| GWB-8982EA | GenWay Biotech | 0.1 mg | Ask for price |
BMP1 Over-expression Lysate Product |
|||
| GWB-B9DBA3 | GenWay Biotech | 0.1 mg | Ask for price |
BMP7 Over-expression Lysate Product |
|||
| GWB-CDBF05 | GenWay Biotech | 0.1 mg | Ask for price |
BMP2 Over-expression Lysate Product |
|||
| GWB-301E6A | GenWay Biotech | 0.1 mg | Ask for price |
BMP6 Over-expression Lysate Product |
|||
| GWB-1F3035 | GenWay Biotech | 0.1 mg | Ask for price |
BMP15 (NM_005448) Human Over-expression Lysate |
|||
| E45H07713-2 | EnoGene | each | EUR 395 |
BMP10 (NM_014482) Human Over-expression Lysate |
|||
| E45H09208-2 | EnoGene | each | EUR 395 |
BMP15 (NM_005448) Human Over-expression Lysate |
|||
| LS009210 | BosterBio | 100ug | EUR 628 |
|
Description: Transient overexpression lysate of bone morphogenetic protein 15 (BMP15) |
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BMP10 (NM_014482) Human Over-expression Lysate |
|||
| LS027302 | BosterBio | 100ug | EUR 960 |
|
Description: Transient overexpression lysate of bone morphogenetic protein 10 (BMP10) |
|||
BMP8B Over-expression Lysate Product |
|||
| GWB-FCC50C | GenWay Biotech | 0.1 mg | Ask for price |
BMP2K Over-expression Lysate Product |
|||
| GWB-D01E56 | GenWay Biotech | 0.1 mg | Ask for price |
BMP7 (NM_001719) Human Over-expression Lysate |
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| E45H19693-2 | EnoGene | 20 ug | EUR 395 |
BMP6 (NM_001718) Human Over-expression Lysate |
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| E45H19694-2 | EnoGene | 20 ug | EUR 395 |
BMP3 (NM_001201) Human Over-expression Lysate |
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| E45H19843-2 | EnoGene | 20 ug | EUR 395 |
BMP2 (NM_001200) Human Over-expression Lysate |
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| E45H19844-2 | EnoGene | 20 ug | EUR 395 |
BMP1 (NM_001199) Human Over-expression Lysate |
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| E45H19845-2 | EnoGene | 20 ug | EUR 395 |
BMP5 (NM_021073) Human Over-expression Lysate |
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| E45H17709-2 | EnoGene | 20 ug | EUR 395 |
BMP1 (NM_006129) Human Over-expression Lysate |
|||
| E45H08040-2 | EnoGene | each | EUR 395 |
BMP1 (NM_001199) Human Over-expression Lysate |
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| LS000649 | BosterBio | 100ug | EUR 960 |
|
Description: Transient overexpression lysate of bone morphogenetic protein 1 (BMP1), transcript variant BMP1-1 |
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BMP2 (NM_001200) Human Over-expression Lysate |
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| LS000650 | BosterBio | 100ug | EUR 628 |
|
Description: Transient overexpression lysate of bone morphogenetic protein 2 (BMP2) |
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BMP3 (NM_001201) Human Over-expression Lysate |
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| LS000651 | BosterBio | 100ug | EUR 960 |
|
Description: Transient overexpression lysate of bone morphogenetic protein 3 (BMP3) |
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BMP5 (NM_021073) Human Over-expression Lysate |
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| LS000653 | BosterBio | 100ug | EUR 628 |
|
Description: Transient overexpression lysate of bone morphogenetic protein 5 (BMP5) |
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BMP6 (NM_001718) Human Over-expression Lysate |
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| LS000654 | BosterBio | 100ug | EUR 960 |
|
Description: Transient overexpression lysate of bone morphogenetic protein 6 (BMP6) |
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BMP7 (NM_001719) Human Over-expression Lysate |
|||
| LS000655 | BosterBio | 100ug | EUR 628 |
|
Description: Transient overexpression lysate of bone morphogenetic protein 7 (BMP7) |
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BMP2K (NM_198892) Human Over-expression Lysate |
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| E45H16324-2 | EnoGene | each | EUR 395 |
BMP8B (NM_001720) Human Over-expression Lysate |
|||
| E45H05830-2 | EnoGene | each | EUR 395 |
BMP8A (NM_181809) Human Over-expression Lysate |
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| E45H15822-2 | EnoGene | each | EUR 395 |
BMP2K (NM_198892) Human Over-expression Lysate |
|||
| LS055589 | BosterBio | 100ug | EUR 960 |
|
Description: Transient overexpression lysate of BMP2 inducible kinase (BMP2K), transcript variant 1 |
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BMP8A (NM_181809) Human Over-expression Lysate |
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| LS353500 | BosterBio | 100ug | EUR 628 |
|
Description: Transient overexpression lysate of bone morphogenetic protein 8a (BMP8A) |
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BMP8B (NM_001720) Human Over-expression Lysate |
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| LS000656 | BosterBio | 100ug | EUR 628 |
|
Description: Transient overexpression lysate of bone morphogenetic protein 8b (BMP8B) |
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BMPR2 Over-expression Lysate Product |
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| GWB-CB3579 | GenWay Biotech | 0.1 mg | Ask for price |
BMP9 (GDF2) (NM_016204) Human Over-expression Lysate |
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| E45H09984-2 | EnoGene | each | EUR 395 |
BMP9 (GDF2) (NM_016204) Human Over-expression Lysate |
|||
| LS002658 | BosterBio | 100ug | EUR 628 |
|
Description: Transient overexpression lysate of growth differentiation factor 2 (GDF2) |
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BMPR2 (NM_001204) Human Over-expression Lysate |
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| E45H05610-2 | EnoGene | each | EUR 395 |
BMPR2 (NM_001204) Human Over-expression Lysate |
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| LS000659 | BosterBio | 100ug | EUR 628 |
|
Description: Transient overexpression lysate of bone morphogenetic protein receptor, type II (serine/threonine kinase) (BMPR2) |
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BMPR1A (NM_004329) Human Over-expression Lysate |
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| E45H07155-2 | EnoGene | each | EUR 395 |
BMPR1B (NM_001203) Human Over-expression Lysate |
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| E45H05611-2 | EnoGene | each | EUR 395 |
BMPR1A (NM_004329) Human Over-expression Lysate |
|||
| LS000657 | BosterBio | 100ug | EUR 628 |
|
Description: Transient overexpression lysate of bone morphogenetic protein receptor, type IA (BMPR1A) |
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BMPR1B (NM_001203) Human Over-expression Lysate |
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| LS000658 | BosterBio | 100ug | EUR 628 |
|
Description: Transient overexpression lysate of bone morphogenetic protein receptor, type IB (BMPR1B) |
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EP Reagent Biuret Reagent - 100ML |
|||
| 1011601 | Scientific Laboratory Supplies | 1L | EUR 59.4 |
PEROXIDE BLOCKING REAGENT Reagent |
|||
| GWB-Q00308 | GenWay Biotech | 50 ml | Ask for price |
Mouse BMP4 HEK293 Overexpression Lysate |
|||
| MBS8116605-03mg | MyBiosource | 0.3mg | EUR 280 |
Mouse BMP4 HEK293 Overexpression Lysate |
|||
| MBS8116605-5x03mg | MyBiosource | 5x0.3mg | EUR 1205 |
EP Reagent Iodoplatinate Reagent - 200ML |
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| 1046300 | Scientific Laboratory Supplies | 200ML | EUR 502.2 |
EP Reagent Molybdovanadic Reagent - 100ML |
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| 1056700 | Scientific Laboratory Supplies | 100ML | EUR 59.4 |
EP Reagent Methoxyphenylacetic Reagent - 100ML |
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| 1053601 | Scientific Laboratory Supplies | 100ML | EUR 490.05 |
Protein expression |
|||
| CRO-4-Ind | Jotbody | each | Ask for price |
EP Reagent Phosphomolybdotungstic Reagent - 100ML |
|||
| 1065000 | Scientific Laboratory Supplies | 100ML | EUR 226.8 |
RFP expression Adenovirus, in vivo ready |
|||
| AVP001-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 455 |
|
Description: pre-made RFP expression adenovirus, provided in PBS solution. |
|||
CFP expression Adenovirus, in vivo ready |
|||
| AVP002-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 455 |
|
Description: pre-made CFP expression adenovirus, provided in PBS solution. |
|||
GFP expression Adenovirus, in vivo ready |
|||
| AVP011-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 455 |
|
Description: pre-made GFP expression adenovirus, provided in PBS solution. |
|||
YFP expression Adenovirus, in vivo ready |
|||
| AVP012-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 455 |
|
Description: pre-made YFP expression adenovirus, provided in PBS solution. |
|||
BFP expression Adenovirus, in vivo ready |
|||
| AVP017-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 455 |
|
Description: pre-made BFP expression adenovirus, provided in PBS solution. |
|||
AVIDIN/BIOTIN BLOCKING REAGENT Reagent |
|||
| GWB-Q00289 | GenWay Biotech | 30 ml | Ask for price |
Gordon-McLeod Reagent (Oxidase reagent) |
|||
| R026-100ML | EWC Diagnostics | 1 unit | EUR 7.76 |
|
Description: Gordon-McLeod Reagent (Oxidase reagent) |
|||
Oxidase Reagent (Gordon-Macleod Reagent) |
|||
| 76207 | Sisco Laboratories | 100 ml | EUR 3.38 |
|
Description: Part B |
|||
YLEX Expression Kit |
|||
| FYY201-1KT | Yeastern Biotech | 1 kit | Ask for price |
EP Reagent Sulfomolybdic Reagent R3 - 1L |
|||
| 1086500 | Scientific Laboratory Supplies | 1L | EUR 398.25 |
TRIpure Reagent (same as Trizol Reagent) |
|||
| MBS356001-INQUIRE | MyBiosource | INQUIRE | Ask for price |
h OCT4 expression Adenovirus, in vivo ready |
|||
| AVP013-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 455 |
|
Description: pre-made adenovirus express human stem cell factor, OCT4, provided in PBS solution for in vivo application. |
|||
h SOX2 expression Adenovirus, in vivo ready |
|||
| AVP014-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 455 |
|
Description: pre-made adenovirus express human stem cell factor, SOX2, provided in PBS solution for in vivo application. |
|||
293 Expression MAX-1 |
|||
| EXP-711-11 | ACROBIOSYSTEMS | 1ml | EUR 218 |
|
Description: 293 Expression MAX-1 is expressed from - -. |
|||
h LIN28 expression Adenovirus, in vivo ready |
|||
| AVP015-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 455 |
|
Description: pre-made adenovirus express human stem cell factor, LIN28, provided in PBS solution for in vivo application. |
|||
Total RNA Isolation Reagent (Reagent Based) |
|||
| MBS402052-100mL | MyBiosource | 100mL | EUR 235 |
Total RNA Isolation Reagent (Reagent Based) |
|||
| MBS402052-4Reactions | MyBiosource | 4Reactions | EUR 165 |
CNGA2 Expression vector |
|||
| 60642 | BPS Bioscience | 20 µg | EUR 900 |
|
Description: The CNGA2 expression vector is designed to express human cyclic nucleotide-gated cation channel 2 (CNGA2) in mammalian cells. |
|||
CNGA2 Expression vector |
|||
| GWB-PSFA61 | GenWay Biotech | 20ug | Ask for price |
Genomic DNA Isolation Reagent (Reagent Based) |
|||
| MBS402050-100mL | MyBiosource | 100mL | EUR 255 |
Genomic DNA Isolation Reagent (Reagent Based) |
|||
| MBS402050-4Reactions | MyBiosource | 4Reactions | EUR 185 |
pYLEX1 - Expression Vector |
|||
| FYY203-5MG | Yeastern Biotech | 5mg | Ask for price |
LEXSYcon2.1 Expression Kit |
|||
| EGE-1310ble | Jena Bioscience GmbH | 1Kit | EUR 979.85 |
|
Description: for constitutive cytosolic or secretory protein expression |
|||
LEXSYcon2.1 Expression Kit |
|||
| EGE-1310bsd | Jena Bioscience GmbH | 1Kit | EUR 979.85 |
|
Description: for constitutive cytosolic or secretory protein expression |
|||
LEXSYcon2.1 Expression Kit |
|||
| EGE-1310hyg | Jena Bioscience GmbH | 1Kit | EUR 979.85 |
|
Description: for constitutive cytosolic or secretory protein expression |
|||
LEXSYcon2.1 Expression Kit |
|||
| EGE-1310neo | Jena Bioscience GmbH | 1Kit | EUR 979.85 |
|
Description: for constitutive cytosolic or secretory protein expression |
|||
LEXSYcon2.1 Expression Kit |
|||
| EGE-1310pac | Jena Bioscience GmbH | 1Kit | EUR 979.85 |
|
Description: for constitutive cytosolic or secretory protein expression |
|||
LEXSYcon2.1 Expression Kit |
|||
| EGE-1310sat | Jena Bioscience GmbH | 1Kit | EUR 979.85 |
|
Description: for constitutive cytosolic or secretory protein expression |
|||
Blunt E1 Expression Kit |
|||
| abx098098-100l | Abbexa | 100 µl | EUR 712.5 |
Blunt E1 Expression Kit |
|||
| abx098098-10rxns | Abbexa | 10 rxns | EUR 560.4 |
Blunt E1 Expression Kit |
|||
| abx098098-1ml | Abbexa | 1 ml | Ask for price |
Blunt E1 Expression Kit |
|||
| abx098098-200l | Abbexa | 200 µl | Ask for price |
Blunt E2 Expression Kit |
|||
| abx098873-100l | Abbexa | 100 µl | EUR 712.5 |
Blunt E2 Expression Kit |
|||
| abx098873-10rxns | Abbexa | 10 rxns | EUR 560.4 |
Blunt E2 Expression Kit |
|||
| abx098873-1ml | Abbexa | 1 ml | Ask for price |
Blunt E2 Expression Kit |
|||
| abx098873-200l | Abbexa | 200 µl | Ask for price |
RFP expression Adenovirus |
|||
| AVP001 | GenTarget | 1x109 IFU/ml x 200ul | EUR 206.5 |
|
Description: pre-made RFP expression adenovirus, provided in DMEM medium. |
|||
CFP expression Adenovirus |
|||
| AVP002 | GenTarget | 1x109 IFU/ml x 200ul | EUR 206.5 |
|
Description: pre-made CFP expression adenovirus, provided in DMEM medium. |
|||
GFP expression Adenovirus |
|||
| AVP011 | GenTarget | 1x109 IFU/ml x 200ul | EUR 206.5 |
|
Description: pre-made GFP expression adenovirus, provided in DMEM medium. |
|||
YFP expression Adenovirus |
|||
| AVP012 | GenTarget | 1x109 IFU/ml x 200ul | EUR 206.5 |
|
Description: pre-made YFP expression adenovirus, provided in DMEM medium. |
|||
BFP expression Adenovirus |
|||
| AVP017 | GenTarget | 1x109 IFU/ml x 200ul | EUR 206.5 |
|
Description: pre-made BFP expression adenovirus, provided in DMEM medium. |
|||
LEXSinduce3 Expression Kit |
|||
| EGE-1410ble | Jena Bioscience GmbH | 1Kit | EUR 2296.43 |
|
Description: for inducible cytosolic or secretory protein expression |
|||
LEXSinduce3 Expression Kit |
|||
| EGE-1410blecherry | Jena Bioscience GmbH | 1Kit | EUR 2296.43 |
|
Description: for inducible cytosolic or secretory protein expression |
|||
LEXSinduce3 Expression Kit |
|||
| EGE-1410neo | Jena Bioscience GmbH | 1Kit | EUR 2296.43 |
|
Description: for inducible cytosolic or secretory protein expression |
|||
LEXSinduce4 Expression Kit |
|||
| EGE-1420blecherry | Jena Bioscience GmbH | 1Kit | EUR 2296.43 |
|
Description: for inducible cytosolic or secretory protein expression |
|||
Rac1 Expression Vector Set |
|||
| STA-454 | Cell Biolabs | 1 kit | EUR 1765.2 |
|
Description: Rac1 Expression Vector Set contains 3 vectors: Rac wild type, T17N dominant negative mutant, and G12V constitutively active mutant. |
|||
RhoA Expression Vector Set |
|||
| STA-456 | Cell Biolabs | 1 kit | EUR 1765.2 |
|
Description: RhoA Expression Vector Set contains 3 vectors: RhoA wild type, T19N dominant negative mutant, and G14V constitutively active mutant. |
|||
Plant Total RNA Isolation Reagent (Reagent Based) |
|||
| MBS402053-100mL | MyBiosource | 100mL | EUR 245 |
Plant Total RNA Isolation Reagent (Reagent Based) |
|||
| MBS402053-4Reactions | MyBiosource | 4Reactions | EUR 165 |
OPN expression inhibitor 1 |
|||
| HY-146064 | MedChemExpress | 10 mg | EUR 2272.76 |
|
Description: OPN expression inhibitor 1 (Compound 11) is an osteopontin (OPN) expression inhibitor. OPN expression inhibitor 1 can be used for breast cancer research[1]. |
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OPN expression inhibitor 1 |
|||
| T62820-10mg | TargetMol Chemicals | 10mg | Ask for price |
|
Description: OPN expression inhibitor 1 |
|||
OPN expression inhibitor 1 |
|||
| T62820-1g | TargetMol Chemicals | 1g | Ask for price |
|
Description: OPN expression inhibitor 1 |
|||
OPN expression inhibitor 1 |
|||
| T62820-1mg | TargetMol Chemicals | 1mg | Ask for price |
|
Description: OPN expression inhibitor 1 |
|||
OPN expression inhibitor 1 |
|||
| T62820-50mg | TargetMol Chemicals | 50mg | Ask for price |
|
Description: OPN expression inhibitor 1 |
|||
OPN expression inhibitor 1 |
|||
| T62820-5mg | TargetMol Chemicals | 5mg | Ask for price |
|
Description: OPN expression inhibitor 1 |
|||
Cdc42 Expression Vector Set |
|||
| STA-455 | Cell Biolabs | 1 kit | EUR 1765.2 |
|
Description: Cdc42 Expression Vector Set contains 3 vectors: Cdc42 wild type, T17N dominant negative mutant, and Q61L constitutively active mutant. |
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Plant Genomic DNA Isolation Reagent (Reagent Based) |
|||
| MBS402051-100mL | MyBiosource | 100mL | EUR 245 |
Plant Genomic DNA Isolation Reagent (Reagent Based) |
|||
| MBS402051-4Reactions | MyBiosource | 4Reactions | EUR 165 |
h FAP Expression Lentivirus |
|||
| LVP1307 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: FAP, containing a RFP-Blasticidin dual selection marker. |
|||
h CD1 Expression Lentivirus |
|||
| LVP1320 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: CD1, containing a RFP-Blasticidin dual selection marker. |
|||
m FAP Expression Lentivirus |
|||
| LVP1326 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for mouse target: FAP, containing a RFP-Blasticidin dual selection marker. |
|||
h VIM Expression Lentivirus |
|||
| LVP1357 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: VIM, containing a RFP-Blasticidin dual selection marker. |
|||
h PXN Expression Lentivirus |
|||
| LVP1358 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: PXN, containing a RFP-Blasticidin dual selection marker. |
|||
m CD8 Expression Lentivirus |
|||
| LVP1363 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for mouse target: CD8, containing a RFP-Blasticidin dual selection marker. |
|||
h TPO Expression Lentivirus |
|||
| LVP1371 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: TPO, containing a RFP-Blasticidin dual selection marker. |
|||
h WAS Expression Lentivirus |
|||
| LVP1429 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: WAS, containing a RFP-Blasticidin dual selection marker. |
|||
m PGR Expression Lentivirus |
|||
| LVP1455 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 346.5 |
|
Description: Pre-made over-expression lentivirus for mouse target: PGR containing a RFP-Blasticidin dual selection marker. |
|||
h MAG Expression Lentivirus |
|||
| LVP1485 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 346.5 |
|
Description: Pre-made over-expression lentivirus for human target: MAG, containing a RFP-Blasticidin dual selection marker. |
|||
h FST Expression Lentivirus |
|||
| LVP1518 | GenTarget | 1x10^8 IFU/ml x 200ul | EUR 455 |
|
Description: Pre-made lentivirus express human FST gene, containing a RFP-Blasticidin dual selection marker. |
|||
h IL2 Expression Lentivirus |
|||
| LVP293 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
|
Description: Lentivirus express a human IL2 gene with a Blasticidin-RFP fusion marker (Dual selection). |
|||
h ADA Expression Lentivirus |
|||
| LVP923 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
|
Description: Pre-made over-expression lentivirus for human target: ADA, containing a RFP-Blasticidin dual selection marker. |
|||
h PBK Expression Lentivirus |
|||
| LVP095 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
|
Description: Pre-made Lentivirus express human PBK gene, carrying a RFP-Blasticidin fusion marker (Dual selection). |
|||
h IL6 Expression Lentivirus |
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| LVP1144 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
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Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker. |
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h CD4 Expression Lentivirus |
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| LVP1152 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
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Description: Pre-made over-expression lentivirus for human target: CD4, containing a RFP-Blasticidin dual selection marker. |
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h IL4 Expression Lentivirus |
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| LVP1161 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
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Description: Pre-made over-expression lentivirus for human target: IL4, containing a RFP-Blasticidin dual selection marker. |
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h IL7 Expression Lentivirus |
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| LVP1162 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
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Description: Pre-made over-expression lentivirus for human target: IL7, containing a RFP-Blasticidin dual selection marker. |
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pAAV-MCS Expression Vector |
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| MBS169528-001mg | MyBiosource | 0.01mg | EUR 695 |
pAAV-MCS Expression Vector |
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| MBS169528-5x001mg | MyBiosource | 5x0.01mg | EUR 2955 |
pAAV-MCS Expression Vector |
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| VPK-410 | Cell Biolabs | 10 µg | EUR 444 |
FlashBAC - Baculovirus Expression System(24 reactions) |
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| GWB-EB1FF8 | GenWay Biotech | Kit | Ask for price |
FlashBAC - Baculovirus Expression System (96 reactions) |
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| GWB-6C34E3 | GenWay Biotech | Kit | Ask for price |
FlashBAC - Baculovirus Expression System (5 reactions) |
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| GWB-7676BB | GenWay Biotech | Kit | Ask for price |
ExoPure? Reagent (Overall Exosome Isolation, urine) |
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| M1003-25 | Biovision | each | EUR 992.4 |
ExoPure? Reagent (Overall Exosome Isolation, urine) |
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| M1003-50 | Biovision | each | EUR 1083.6 |
h OCT4 expression Adenovirus |
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| AVP013 | GenTarget | 1x109 IFU/ml x 200ul | EUR 206.5 |
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Description: pre-made adenovirus express human stem cell factor, OCT4, provided in DMEM medium. |
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h SOX2 expression Adenovirus |
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| AVP014 | GenTarget | 1x109 IFU/ml x 50ul | EUR 206.5 |
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Description: pre-made adenovirus express human stem cell factor, SOX2, provided in DMEM medium. |
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h CBX7 Expression Lentivirus |
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| LVP1308 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
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Description: Over-expression lentivirus for human target: CBX7, containing a RFP-Blasticidin dual selection marker. |
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h ACE2 Expression Lentivirus |
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| LVP1310 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
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Description: Over-expression lentivirus for human target: ACE2, containing a RFP-Blasticidin dual selection marker. |
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h Rab7 Expression Lentivirus |
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| LVP1315 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
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Description: Over-expression lentivirus for human target: Rab7, containing a RFP-Blasticidin dual selection marker. |
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h Rab5 Expression Lentivirus |
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| LVP1316 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
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Description: Over-expression lentivirus for human target: Rab5, containing a RFP-Blasticidin dual selection marker. |
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m ASPA Expression Lentivirus |
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| LVP1317 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
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Description: Over-expression lentivirus for mouse target: ASPA, containing a RFP-Blasticidin dual selection marker. |
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h CD44 Expression Lentivirus |
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| LVP1318 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
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Description: Over-expression lentivirus for human target: CD44, containing a RFP-Blasticidin dual selection marker. |
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h MFN2 Expression Lentivirus |
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| LVP1327 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
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Description: Over-expression lentivirus for human target: MFN2, containing a RFP-Blasticidin dual selection marker. |
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h ANO1 Expression Lentivirus |
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| LVP1344 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
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Description: Over-expression lentivirus for human target: ANO1 containing a RFP-Blasticidin dual selection marker. |
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h FGL2 Expression Lentivirus |
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| LVP1346 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
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Description: Over-expression lentivirus for human target: FGL2, containing a RFP-Blasticidin dual selection marker. |
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h CCR2 Expression Lentivirus |
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| LVP1354 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
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Description: Over-expression lentivirus for human target: CCR2, containing a RFP-Blasticidin dual selection marker. |
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h NEFL Expression Lentivirus |
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| LVP1360 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
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Description: Over-expression lentivirus for human target: NEFL, containing a RFP-Blasticidin dual selection marker. |
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h NOS2 Expression Lentivirus |
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| LVP1367 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
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Description: Over-expression lentivirus for human target: NOS2, containing a RFP-Blasticidin dual selection marker. |
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h BMI1 Expression Lentivirus |
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| LVP1372 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
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Description: Over-expression lentivirus for human target: BMI1, containing a RFP-Blasticidin dual selection marker. |
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h PSCA Expression Lentivirus |
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| LVP1374 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
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Description: Over-expression lentivirus for human target: PSCA, containing a RFP-Blasticidin dual selection marker. |
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h POLQ Expression Lentivirus |
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| LVP1421 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
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Description: Over-expression lentivirus for human target: POLQ containing a RFP-Blasticidin dual selection marker. |
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h ARSA Expression Lentivirus |
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| LVP1425 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
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Description: Over-expression lentivirus for human target: ARSA, containing a RFP-Blasticidin dual selection marker. |
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h IDUA Expression Lentivirus |
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| LVP1426 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
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Description: Over-expression lentivirus for human target: IDUA, containing a RFP-Blasticidin dual selection marker. |
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h SGSH Expression Lentivirus |
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| LVP1427 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
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Description: Over-expression lentivirus for human target: SGSH, containing a RFP-Blasticidin dual selection marker. |
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h CYBB Expression Lentivirus |
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| LVP1430 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
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Description: Over-expression lentivirus for human target: CYBB, containing a RFP-Blasticidin dual selection marker. |
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h CAV1 Expression Lentivirus |
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| LVP1431 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
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Description: Over-expression lentivirus for human target: CAV1, containing a RFP-Blasticidin dual selection marker. |
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m INS2 Expression Lentivirus |
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| LVP1456 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made over-expression lentivirus for mouse target: INS2 containing a RFP-Blasticidin dual selection marker. |
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h ROR1 Expression Lentivirus |
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| LVP1458 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made over-expression lentivirus for human target: ROR1, containing a RFP-Blasticidin dual selection marker. |
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h DGKA Expression Lentivirus |
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| LVP1468 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made over-expression lentivirus for human target: DGKA, containing a RFP-Blasticidin dual selection marker. |
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h REST_HA Expression Lentivirus |
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| LVP1475 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made over-expression lentivirus for human target: REST, containing a RFP-Blasticidin dual selection marker. |
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h UBR7 Expression Lentivirus |
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| LVP1483 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made over-expression lentivirus for human target: UBR7, containing a RFP-Blasticidin dual selection marker. |
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h MMP1 Expression Lentivirus |
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| LVP1516 | GenTarget | 1x10^8 IFU/ml x 200ul | EUR 455 |
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Description: Pre-made lentivirus express human MMP1 gene, containing a RFP-Blasticidin dual selection marker. |
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h BDNF Expression Lentivirus |
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| LVP1520 | GenTarget | 1x10^8 IFU/ml x 200ul | EUR 455 |
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Description: Pre-made lentivirus express human BDNF gene, containing a RFP-Blasticidin dual selection marker. |
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RBDv1 contained a local viral sign peptide, RBDv2 -human tPA sign peptide. We transfected a CHO DG44 cell line, chosen stably transfected cells, and carried out just a few rounds of methotrexate-driven amplification of the genetic cassette within the genome. For the RBDv2 variant, a high-yield clonal producer cell line was obtained. Purified proteins had been analyzed by polyacrylamide gel electrophoresis in lowering and non-reducing situations and gel filtration; for RBDv2 protein, the monomeric type content material exceeded 90% for a number of sequence. Deglycosylation with PNGase F and mass spectrometry confirmed the presence of N-glycosylation.