In our earlier research, a novel gene remedy method was developed based mostly on a plasmid vector pSecTag2B through which recombinant HNP1 gene was regulated below a cytomegalovirus promoter to encode a mature HNP1 type. We confirmed for the primary time in numerous tumor fashions together with human most cancers xenografts that overexpression of HNP1 within the tumor milieu by intratumoral pSecTag-HNP1 (pHNP1) administration effectively attenuated in vivo tumor development, mediated host immune responses to tumors, and produced a synergistic impact when mixed with chemotherapeutics.
In present research, a preclinical security investigation of HNP1 gene remedy was carried out in non-human primates. Eleven cynomolgus monkeys had been divided into three teams of three to four animals every and obtained both repeated s.c. injections of pHNP1/cationic liposome complexes at low (0.625 mg/kg) or excessive (2.5 mg/kg) dose or glucose as management. Important HNP1 in vivo accumulation was detected after consecutive administrations. All primates reached the top of the research with good physique situations. Injection web site irritation was the one apparent poisonous response throughout commentary interval.
As well as, elevation of monocyte/macrophage and neutrophil in addition to decline of lymphocyte had been detected within the peripheral blood of pHNP1-treated primates. These alterations had been partially alleviated on the finish of commentary interval. In addition to, dose-related histopathological adjustments of the immune organs had been noticed at necropsy, together with a minimal thymic lymphocyte lower and a minimal-to-mild lymph node erythrocyte enhance, however which can’t be excluded from HNP1 induced immune reactions. Collectively, these information help future medical research of pHNP1-based native gene supply in tumor sufferers.
The downregulation of endogenous dhfr improved the effectivity of EBNA-1 amplification, as evidenced by a comparability with the amplification extent in cells missing shRNA expression on the similar MTX focus. The EBNA-1 expression ranges from the EBNA-1-amplified clones chosen on this research had been larger than these obtained from EBNA-1-amplified clones that had been generated utilizing the standard amplification in our earlier research. According to earlier research, EBNA-1 amplification improved the manufacturing of the Fc-fusion protein by a selected protein productiveness (qp)-enhancing impact, slightly than by bettering cell development or transfection effectivity.
Joint common modular plasmids (JUMP): a versatile vector platform for artificial biology
Technology of latest DNA constructs is an important course of in trendy life science and biotechnology. Modular cloning techniques based mostly on Golden Gate cloning, utilizing Kind IIS restriction endonucleases, permit meeting of complicated multipart constructs from reusable fundamental DNA components in a fast, dependable and automation-friendly manner. Many such toolkits can be found, with various levels of compatibility, most of that are aimed toward particular host organisms. Right here, we current a vector design which permits easy vector modification by utilizing modular cloning to assemble and add new capabilities in secondary websites flanking the primary insertion web site (used for typical modular cloning).
Meeting in all websites is suitable with the PhytoBricks normal, and vectors are suitable with the Commonplace European Vector Structure (SEVA) in addition to BioBricks. We exhibit that this facilitates the development of vectors with tailor-made capabilities and simplifies the workflow for producing libraries of constructs with frequent components. We have now made out there a set of vectors with 10 totally different microbial replication origins, various in copy quantity and host vary, and permitting chromosomal integration, in addition to a choice of generally used fundamental components.
This design expands the vary of hosts which might be simply modified by modular cloning and acts as a toolkit which can be utilized to facilitate the technology of latest toolkits with particular capabilities required for focusing on additional hosts. The antigen produced by the described approach is appropriate for serological exams and subunit vaccine research. We developed a easy purification scheme that constantly yielded as much as 30 mg of RBD protein per liter of the easy shake flask cell tradition.
Excessive-level expression of the monomeric SARS-CoV-2 S protein RBD 320-537 in stably transfected CHO cells by the EEF1A1-based plasmid vector
The spike (S) protein is likely one of the three proteins forming the coronaviruses’ viral envelope. The S protein of the Extreme Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial construction much like the S proteins of different mammalian coronaviruses, aside from a singular receptor-binding area (RBD), which is a big inducer of host immune response. Recombinant SARS-CoV-2 RBD is broadly used as a extremely particular minimal antigen for serological exams.
Right publicity of antigenic determinants has a big affect on the accuracy of such tests-the antigen must be appropriately folded, comprise no probably antigenic non-vertebrate glycans, and, ideally, ought to have a glycosylation sample much like the native S protein. Based mostly on the beforehand developed p1.1 vector, containing the regulatory sequences of the Eukaryotic translation elongation issue 1 alpha gene (EEF1A1) from Chinese language hamster, we created two expression constructs encoding SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine tags.
BMP3 Over-expression Lysate Product |
|||
GWB-8982EA | GenWay Biotech | 0.1 mg | Ask for price |
BMP1 Over-expression Lysate Product |
|||
GWB-B9DBA3 | GenWay Biotech | 0.1 mg | Ask for price |
BMP7 Over-expression Lysate Product |
|||
GWB-CDBF05 | GenWay Biotech | 0.1 mg | Ask for price |
BMP8B Over-expression Lysate Product |
|||
GWB-FCC50C | GenWay Biotech | 0.1 mg | Ask for price |
BMP2K Over-expression Lysate Product |
|||
GWB-D01E56 | GenWay Biotech | 0.1 mg | Ask for price |
BMPR2 Over-expression Lysate Product |
|||
GWB-CB3579 | GenWay Biotech | 0.1 mg | Ask for price |
h BMP2 Expression, Concentrated Lentivirus |
|||
LVP481-PBS | GenTarget | 1x10^8 IFU/ml x 200ul | EUR 455 |
Description: Pre-made express human target: BMP2 gene, carrying RFP-Blasticidin dual selection. |
NEU3 Over-expression Lysate |
|||
GWB-DL2468 | GenWay Biotech | 0.1 mg | Ask for price |
Protein expression |
|||
CRO-4-Ind | Jotbody | each | Ask for price |
YLEX Expression Kit |
|||
FYY201-1KT | Yeastern Biotech | 1 kit | Ask for price |
RFP expression Adenovirus, in vivo ready |
|||
AVP001-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 852 |
Description: pre-made RFP expression adenovirus, provided in PBS solution. |
CFP expression Adenovirus, in vivo ready |
|||
AVP002-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 852 |
Description: pre-made CFP expression adenovirus, provided in PBS solution. |
GFP expression Adenovirus, in vivo ready |
|||
AVP011-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 852 |
Description: pre-made GFP expression adenovirus, provided in PBS solution. |
YFP expression Adenovirus, in vivo ready |
|||
AVP012-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 852 |
Description: pre-made YFP expression adenovirus, provided in PBS solution. |
BFP expression Adenovirus, in vivo ready |
|||
AVP017-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 852 |
Description: pre-made BFP expression adenovirus, provided in PBS solution. |
EP Reagent Biuret Reagent - 100ML |
|||
1011601 | Scientific Laboratory Supplies | 1L | EUR 59.4 |
293 Expression MAX-1 |
|||
EXP-711-11 | ACROBIOSYSTEMS | 1ml | EUR 214 |
Description: 293 Expression MAX-1 is expressed from - -. |
PEROXIDE BLOCKING REAGENT Reagent |
|||
GWB-Q00308 | GenWay Biotech | 50 ml | Ask for price |
CNGA2 Expression vector |
|||
60642 | BPS Bioscience | 20 µg | EUR 900 |
Description: The CNGA2 expression vector is designed to express human cyclic nucleotide-gated cation channel 2 (CNGA2) in mammalian cells. |
CNGA2 Expression vector |
|||
GWB-PSFA61 | GenWay Biotech | 20ug | Ask for price |
EP Reagent Iodoplatinate Reagent - 200ML |
|||
1046300 | Scientific Laboratory Supplies | 200ML | EUR 502.2 |
LEXSYcon2.1 Expression Kit |
|||
EGE-1310ble | Jena Bioscience GmbH | 1Kit | EUR 1296.1 |
Description: for constitutive cytosolic or secretory protein expression |
LEXSYcon2.1 Expression Kit |
|||
EGE-1310bsd | Jena Bioscience GmbH | 1Kit | EUR 1296.1 |
Description: for constitutive cytosolic or secretory protein expression |
LEXSYcon2.1 Expression Kit |
|||
EGE-1310hyg | Jena Bioscience GmbH | 1Kit | EUR 1296.1 |
Description: for constitutive cytosolic or secretory protein expression |
LEXSYcon2.1 Expression Kit |
|||
EGE-1310neo | Jena Bioscience GmbH | 1Kit | EUR 1296.1 |
Description: for constitutive cytosolic or secretory protein expression |
LEXSYcon2.1 Expression Kit |
|||
EGE-1310pac | Jena Bioscience GmbH | 1Kit | EUR 1296.1 |
Description: for constitutive cytosolic or secretory protein expression |
LEXSYcon2.1 Expression Kit |
|||
EGE-1310sat | Jena Bioscience GmbH | 1Kit | EUR 1296.1 |
Description: for constitutive cytosolic or secretory protein expression |
pYLEX1 - Expression Vector |
|||
FYY203-5MG | Yeastern Biotech | 5mg | Ask for price |
h OCT4 expression Adenovirus, in vivo ready |
|||
AVP013-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 852 |
Description: pre-made adenovirus express human stem cell factor, OCT4, provided in PBS solution for in vivo application. |
h SOX2 expression Adenovirus, in vivo ready |
|||
AVP014-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 852 |
Description: pre-made adenovirus express human stem cell factor, SOX2, provided in PBS solution for in vivo application. |
EP Reagent Molybdovanadic Reagent - 100ML |
|||
1056700 | Scientific Laboratory Supplies | 100ML | EUR 59.4 |
Blunt E1 Expression Kit |
|||
abx098098-10rxns | Abbexa | 10 rxns | EUR 560.4 |
Blunt E2 Expression Kit |
|||
abx098873-10rxns | Abbexa | 10 rxns | EUR 560.4 |
Blunt E1 Expression Kit |
|||
abx098098-100l | Abbexa | 100 µl | EUR 712.5 |
Blunt E1 Expression Kit |
|||
abx098098-1ml | Abbexa | 1 ml | Ask for price |
Blunt E1 Expression Kit |
|||
abx098098-200l | Abbexa | 200 µl | Ask for price |
Blunt E2 Expression Kit |
|||
abx098873-100l | Abbexa | 100 µl | EUR 712.5 |
Blunt E2 Expression Kit |
|||
abx098873-1ml | Abbexa | 1 ml | Ask for price |
Blunt E2 Expression Kit |
|||
abx098873-200l | Abbexa | 200 µl | Ask for price |
RFP expression Adenovirus |
|||
AVP001 | GenTarget | 1x109 IFU/ml x 200ul | EUR 418.8 |
Description: pre-made RFP expression adenovirus, provided in DMEM medium. |
CFP expression Adenovirus |
|||
AVP002 | GenTarget | 1x109 IFU/ml x 200ul | EUR 418.8 |
Description: pre-made CFP expression adenovirus, provided in DMEM medium. |
GFP expression Adenovirus |
|||
AVP011 | GenTarget | 1x109 IFU/ml x 200ul | EUR 418.8 |
Description: pre-made GFP expression adenovirus, provided in DMEM medium. |
YFP expression Adenovirus |
|||
AVP012 | GenTarget | 1x109 IFU/ml x 200ul | EUR 418.8 |
Description: pre-made YFP expression adenovirus, provided in DMEM medium. |
BFP expression Adenovirus |
|||
AVP017 | GenTarget | 1x109 IFU/ml x 200ul | EUR 418.8 |
Description: pre-made BFP expression adenovirus, provided in DMEM medium. |
RPL21_17_477 Over-expression Lysate Product |
|||
GWB-8BD971 | GenWay Biotech | 0.1 mg | Ask for price |
h LIN28 expression Adenovirus, in vivo ready |
|||
AVP015-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 852 |
Description: pre-made adenovirus express human stem cell factor, LIN28, provided in PBS solution for in vivo application. |
LEXSinduce3 Expression Kit |
|||
EGE-1410ble | Jena Bioscience GmbH | 1Kit | EUR 3037.6 |
Description: for inducible cytosolic or secretory protein expression |
LEXSinduce3 Expression Kit |
|||
EGE-1410blecherry | Jena Bioscience GmbH | 1Kit | EUR 3037.6 |
Description: for inducible cytosolic or secretory protein expression |
LEXSinduce3 Expression Kit |
|||
EGE-1410neo | Jena Bioscience GmbH | 1Kit | EUR 3037.6 |
Description: for inducible cytosolic or secretory protein expression |
LEXSinduce4 Expression Kit |
|||
EGE-1420blecherry | Jena Bioscience GmbH | 1Kit | EUR 3037.6 |
Description: for inducible cytosolic or secretory protein expression |
EP Reagent Methoxyphenylacetic Reagent - 100ML |
|||
1053601 | Scientific Laboratory Supplies | 100ML | EUR 490.05 |
F8 Over-expression Lysate Product |
|||
GWB-4223CC | GenWay Biotech | 0.1 mg | Ask for price |
AR Over-expression Lysate Product |
|||
GWB-524326 | GenWay Biotech | 0.1 mg | Ask for price |
MB Over-expression Lysate Product |
|||
GWB-543EE8 | GenWay Biotech | 0.1 mg | Ask for price |
C5 Over-expression Lysate Product |
|||
GWB-611C50 | GenWay Biotech | 0.1 mg | Ask for price |
F7 Over-expression Lysate Product |
|||
GWB-2B9655 | GenWay Biotech | 0.1 mg | Ask for price |
HP Over-expression Lysate Product |
|||
GWB-19D90B | GenWay Biotech | 0.1 mg | Ask for price |
C9 Over-expression Lysate Product |
|||
GWB-A98C50 | GenWay Biotech | 0.1 mg | Ask for price |
GK Over-expression Lysate Product |
|||
GWB-ABEC99 | GenWay Biotech | 0.1 mg | Ask for price |
C6 Over-expression Lysate Product |
|||
GWB-4CD0B5 | GenWay Biotech | 0.1 mg | Ask for price |
T Over-expression Lysate Product |
|||
GWB-4D17A7 | GenWay Biotech | 0.1 mg | Ask for price |
C2 Over-expression Lysate Product |
|||
GWB-69AF56 | GenWay Biotech | 0.1 mg | Ask for price |
C7 Over-expression Lysate Product |
|||
GWB-6F310B | GenWay Biotech | 0.1 mg | Ask for price |
GC Over-expression Lysate Product |
|||
GWB-6FD425 | GenWay Biotech | 0.1 mg | Ask for price |
F2 Over-expression Lysate Product |
|||
GWB-74B4A0 | GenWay Biotech | 0.1 mg | Ask for price |
GK Over-expression Lysate Product |
|||
GWB-799C20 | GenWay Biotech | 0.1 mg | Ask for price |
F9 Over-expression Lysate Product |
|||
GWB-7A1142 | GenWay Biotech | 0.1 mg | Ask for price |
TF Over-expression Lysate Product |
|||
GWB-FDE7E0 | GenWay Biotech | 0.1 mg | Ask for price |
FH Over-expression Lysate Product |
|||
GWB-B00EEC | GenWay Biotech | 0.1 mg | Ask for price |
KL Over-expression Lysate Product |
|||
GWB-EF6FA2 | GenWay Biotech | 0.1 mg | Ask for price |
MB Over-expression Lysate Product |
|||
GWB-DDB100 | GenWay Biotech | 0.1 mg | Ask for price |
XG Over-expression Lysate Product |
|||
GWB-C19FF5 | GenWay Biotech | 0.1 mg | Ask for price |
PC Over-expression Lysate Product |
|||
GWB-C40C56 | GenWay Biotech | 0.1 mg | Ask for price |
Rac1 Expression Vector Set |
|||
STA-454 | Cell Biolabs | 1 kit | EUR 1765.2 |
Description: Rac1 Expression Vector Set contains 3 vectors: Rac wild type, T17N dominant negative mutant, and G12V constitutively active mutant. |
RhoA Expression Vector Set |
|||
STA-456 | Cell Biolabs | 1 kit | EUR 1765.2 |
Description: RhoA Expression Vector Set contains 3 vectors: RhoA wild type, T19N dominant negative mutant, and G14V constitutively active mutant. |
CRX Over-expression Lysate Product |
|||
GWB-372B50 | GenWay Biotech | 0.1 mg | Ask for price |
FST Over-expression Lysate Product |
|||
GWB-3758FE | GenWay Biotech | 0.1 mg | Ask for price |
PNN Over-expression Lysate Product |
|||
GWB-379ABB | GenWay Biotech | 0.1 mg | Ask for price |
CRK Over-expression Lysate Product |
|||
GWB-37B7E4 | GenWay Biotech | 0.1 mg | Ask for price |
B2M Over-expression Lysate Product |
|||
GWB-39B4E3 | GenWay Biotech | 0.1 mg | Ask for price |
NGB Over-expression Lysate Product |
|||
GWB-39F54D | GenWay Biotech | 0.1 mg | Ask for price |
KIN Over-expression Lysate Product |
|||
GWB-3ABEBE | GenWay Biotech | 0.1 mg | Ask for price |
OXT Over-expression Lysate Product |
|||
GWB-3B1FD3 | GenWay Biotech | 0.1 mg | Ask for price |
PXK Over-expression Lysate Product |
|||
GWB-3C8EA1 | GenWay Biotech | 0.1 mg | Ask for price |
ALB Over-expression Lysate Product |
|||
GWB-3E03B0 | GenWay Biotech | 0.1 mg | Ask for price |
IL9 Over-expression Lysate Product |
|||
GWB-3E3A84 | GenWay Biotech | 0.1 mg | Ask for price |
HLA Over-expression Lysate Product |
|||
GWB-3E6FB0 | GenWay Biotech | 0.1 mg | Ask for price |
SPR Over-expression Lysate Product |
|||
GWB-3FACD1 | GenWay Biotech | 0.1 mg | Ask for price |
SPN Over-expression Lysate Product |
|||
GWB-404D95 | GenWay Biotech | 0.1 mg | Ask for price |
AIP Over-expression Lysate Product |
|||
GWB-40DF2C | GenWay Biotech | 0.1 mg | Ask for price |
CSK Over-expression Lysate Product |
|||
GWB-415B1A | GenWay Biotech | 0.1 mg | Ask for price |
WT1 Over-expression Lysate Product |
|||
GWB-41C1E8 | GenWay Biotech | 0.1 mg | Ask for price |
C8B Over-expression Lysate Product |
|||
GWB-41E9AB | GenWay Biotech | 0.1 mg | Ask for price |
HDC Over-expression Lysate Product |
|||
GWB-4302B9 | GenWay Biotech | 0.1 mg | Ask for price |
TNF Over-expression Lysate Product |
|||
GWB-448BBB | GenWay Biotech | 0.1 mg | Ask for price |
PNP Over-expression Lysate Product |
|||
GWB-4538EC | GenWay Biotech | 0.1 mg | Ask for price |
MUT Over-expression Lysate Product |
|||
GWB-455C37 | GenWay Biotech | 0.1 mg | Ask for price |
CFP Over-expression Lysate Product |
|||
GWB-4575E9 | GenWay Biotech | 0.1 mg | Ask for price |
HPN Over-expression Lysate Product |
|||
GWB-4602DE | GenWay Biotech | 0.1 mg | Ask for price |
C1D Over-expression Lysate Product |
|||
GWB-461FBD | GenWay Biotech | 0.1 mg | Ask for price |
PIP Over-expression Lysate Product |
|||
GWB-4782A0 | GenWay Biotech | 0.1 mg | Ask for price |
SF1 Over-expression Lysate Product |
|||
GWB-47A9D3 | GenWay Biotech | 0.1 mg | Ask for price |
DDO Over-expression Lysate Product |
|||
GWB-481A81 | GenWay Biotech | 0.1 mg | Ask for price |
LYZ Over-expression Lysate Product |
|||
GWB-4830E7 | GenWay Biotech | 0.1 mg | Ask for price |
PAM Over-expression Lysate Product |
|||
GWB-48AFF2 | GenWay Biotech | 0.1 mg | Ask for price |
DCN Over-expression Lysate Product |
|||
GWB-4AB343 | GenWay Biotech | 0.1 mg | Ask for price |
MVD Over-expression Lysate Product |
|||
GWB-4B1DAE | GenWay Biotech | 0.1 mg | Ask for price |
CPM Over-expression Lysate Product |
|||
GWB-4B1F3E | GenWay Biotech | 0.1 mg | Ask for price |
REN Over-expression Lysate Product |
|||
GWB-4B825B | GenWay Biotech | 0.1 mg | Ask for price |
HRK Over-expression Lysate Product |
|||
GWB-4BB955 | GenWay Biotech | 0.1 mg | Ask for price |
SHD Over-expression Lysate Product |
|||
GWB-4BFEE6 | GenWay Biotech | 0.1 mg | Ask for price |
GH1 Over-expression Lysate Product |
|||
GWB-4F3DD0 | GenWay Biotech | 0.1 mg | Ask for price |
IVD Over-expression Lysate Product |
|||
GWB-4F555F | GenWay Biotech | 0.1 mg | Ask for price |
SLA Over-expression Lysate Product |
|||
GWB-4F6FFE | GenWay Biotech | 0.1 mg | Ask for price |
AK4 Over-expression Lysate Product |
|||
GWB-4FADC0 | GenWay Biotech | 0.1 mg | Ask for price |
BRE Over-expression Lysate Product |
|||
GWB-4FC6EC | GenWay Biotech | 0.1 mg | Ask for price |
MOS Over-expression Lysate Product |
|||
GWB-503237 | GenWay Biotech | 0.1 mg | Ask for price |
ERF Over-expression Lysate Product |
|||
GWB-5242BB | GenWay Biotech | 0.1 mg | Ask for price |
HFE Over-expression Lysate Product |
|||
GWB-5243B9 | GenWay Biotech | 0.1 mg | Ask for price |
TEK Over-expression Lysate Product |
|||
GWB-533C4C | GenWay Biotech | 0.1 mg | Ask for price |
MIA Over-expression Lysate Product |
|||
GWB-534100 | GenWay Biotech | 0.1 mg | Ask for price |
IDE Over-expression Lysate Product |
|||
GWB-5367D7 | GenWay Biotech | 0.1 mg | Ask for price |
AFP Over-expression Lysate Product |
|||
GWB-536D51 | GenWay Biotech | 0.1 mg | Ask for price |
RDX Over-expression Lysate Product |
|||
GWB-53CF80 | GenWay Biotech | 0.1 mg | Ask for price |
ILK Over-expression Lysate Product |
|||
GWB-54E227 | GenWay Biotech | 0.1 mg | Ask for price |
SFN Over-expression Lysate Product |
|||
GWB-55C5F7 | GenWay Biotech | 0.1 mg | Ask for price |
BPI Over-expression Lysate Product |
|||
GWB-55FF0C | GenWay Biotech | 0.1 mg | Ask for price |
MAX Over-expression Lysate Product |
|||
GWB-56E43C | GenWay Biotech | 0.1 mg | Ask for price |
TAZ Over-expression Lysate Product |
|||
GWB-572853 | GenWay Biotech | 0.1 mg | Ask for price |
MLN Over-expression Lysate Product |
|||
GWB-576AA3 | GenWay Biotech | 0.1 mg | Ask for price |
CA2 Over-expression Lysate Product |
|||
GWB-578B82 | GenWay Biotech | 0.1 mg | Ask for price |
GIP Over-expression Lysate Product |
|||
GWB-57B640 | GenWay Biotech | 0.1 mg | Ask for price |
MKX Over-expression Lysate Product |
|||
GWB-57FD34 | GenWay Biotech | 0.1 mg | Ask for price |
FAS Over-expression Lysate Product |
|||
GWB-586DE6 | GenWay Biotech | 0.1 mg | Ask for price |
ADK Over-expression Lysate Product |
|||
GWB-590E04 | GenWay Biotech | 0.1 mg | Ask for price |
CAT Over-expression Lysate Product |
|||
GWB-59B4AF | GenWay Biotech | 0.1 mg | Ask for price |
AES Over-expression Lysate Product |
|||
GWB-5A4211 | GenWay Biotech | 0.1 mg | Ask for price |
GRP Over-expression Lysate Product |
|||
GWB-5A56CC | GenWay Biotech | 0.1 mg | Ask for price |
C8A Over-expression Lysate Product |
|||
GWB-5AD920 | GenWay Biotech | 0.1 mg | Ask for price |
ME2 Over-expression Lysate Product |
|||
GWB-5B40C3 | GenWay Biotech | 0.1 mg | Ask for price |
TTK Over-expression Lysate Product |
|||
GWB-5B4DBF | GenWay Biotech | 0.1 mg | Ask for price |
HGF Over-expression Lysate Product |
|||
GWB-5CC3A0 | GenWay Biotech | 0.1 mg | Ask for price |
XDH Over-expression Lysate Product |
|||
GWB-5CD953 | GenWay Biotech | 0.1 mg | Ask for price |
DES Over-expression Lysate Product |
|||
GWB-5E571D | GenWay Biotech | 0.1 mg | Ask for price |
LOX Over-expression Lysate Product |
|||
GWB-5FE87A | GenWay Biotech | 0.1 mg | Ask for price |
BMX Over-expression Lysate Product |
|||
GWB-603D44 | GenWay Biotech | 0.1 mg | Ask for price |
GAA Over-expression Lysate Product |
|||
GWB-611DD0 | GenWay Biotech | 0.1 mg | Ask for price |
DR1 Over-expression Lysate Product |
|||
GWB-62642D | GenWay Biotech | 0.1 mg | Ask for price |
ABR Over-expression Lysate Product |
|||
GWB-62B984 | GenWay Biotech | 0.1 mg | Ask for price |
CA3 Over-expression Lysate Product |
|||
GWB-62F5B6 | GenWay Biotech | 0.1 mg | Ask for price |
FUZ Over-expression Lysate Product |
|||
GWB-635D50 | GenWay Biotech | 0.1 mg | Ask for price |
HGD Over-expression Lysate Product |
|||
GWB-64C9ED | GenWay Biotech | 0.1 mg | Ask for price |
JTB Over-expression Lysate Product |
|||
GWB-655583 | GenWay Biotech | 0.1 mg | Ask for price |
FAH Over-expression Lysate Product |
|||
GWB-65575F | GenWay Biotech | 0.1 mg | Ask for price |
LPP Over-expression Lysate Product |
|||
GWB-1EE6BD | GenWay Biotech | 0.1 mg | Ask for price |
QKI Over-expression Lysate Product |
|||
GWB-2019C5 | GenWay Biotech | 0.1 mg | Ask for price |
HBB Over-expression Lysate Product |
|||
GWB-2046D5 | GenWay Biotech | 0.1 mg | Ask for price |
ZAK Over-expression Lysate Product |
|||
GWB-20AE0D | GenWay Biotech | 0.1 mg | Ask for price |
PF4 Over-expression Lysate Product |
|||
GWB-212C3B | GenWay Biotech | 0.1 mg | Ask for price |
TFG Over-expression Lysate Product |
|||
GWB-215F68 | GenWay Biotech | 0.1 mg | Ask for price |
ACE Over-expression Lysate Product |
|||
GWB-21AE7F | GenWay Biotech | 0.1 mg | Ask for price |
ERH Over-expression Lysate Product |
|||
GWB-220D0B | GenWay Biotech | 0.1 mg | Ask for price |
GIF Over-expression Lysate Product |
|||
GWB-23C77E | GenWay Biotech | 0.1 mg | Ask for price |
PGD Over-expression Lysate Product |
|||
GWB-242ED0 | GenWay Biotech | 0.1 mg | Ask for price |
GSN Over-expression Lysate Product |
|||
GWB-2435DF | GenWay Biotech | 0.1 mg | Ask for price |
HLA Over-expression Lysate Product |
|||
GWB-2494DC | GenWay Biotech | 0.1 mg | Ask for price |
RBDv1 contained a local viral sign peptide, RBDv2 -human tPA sign peptide. We transfected a CHO DG44 cell line, chosen stably transfected cells, and carried out just a few rounds of methotrexate-driven amplification of the genetic cassette within the genome. For the RBDv2 variant, a high-yield clonal producer cell line was obtained. Purified proteins had been analyzed by polyacrylamide gel electrophoresis in lowering and non-reducing situations and gel filtration; for RBDv2 protein, the monomeric type content material exceeded 90% for a number of sequence. Deglycosylation with PNGase F and mass spectrometry confirmed the presence of N-glycosylation.