Preclinical safety evaluation of a recombinant plasmid vector encoding mature human neutrophil peptide-1 by repeated local administrations in nonhuman primates

Preclinical safety evaluation of a recombinant plasmid vector encoding mature human neutrophil peptide-1 by repeated local administrations in nonhuman primates

In our earlier research, a novel gene remedy method was developed based mostly on a plasmid vector pSecTag2B through which recombinant HNP1 gene was regulated below a cytomegalovirus promoter to encode a mature HNP1 type. We confirmed for the primary time in numerous tumor fashions together with human most cancers xenografts that overexpression of HNP1 within the tumor milieu by intratumoral pSecTag-HNP1 (pHNP1) administration effectively attenuated in vivo tumor development, mediated host immune responses to tumors, and produced a synergistic impact when mixed with chemotherapeutics.

In present research, a preclinical security investigation of HNP1 gene remedy was carried out in non-human primates. Eleven cynomolgus monkeys had been divided into three teams of three to four animals every and obtained both repeated s.c. injections of pHNP1/cationic liposome complexes at low (0.625 mg/kg) or excessive (2.5 mg/kg) dose or glucose as management. Important HNP1 in vivo accumulation was detected after consecutive administrations. All primates reached the top of the research with good physique situations. Injection web site irritation was the one apparent poisonous response throughout commentary interval.

As well as, elevation of monocyte/macrophage and neutrophil in addition to decline of lymphocyte had been detected within the peripheral blood of pHNP1-treated primates. These alterations had been partially alleviated on the finish of commentary interval. In addition to, dose-related histopathological adjustments of the immune organs had been noticed at necropsy, together with a minimal thymic lymphocyte lower and a minimal-to-mild lymph node erythrocyte enhance, however which can’t be excluded from HNP1 induced immune reactions. Collectively, these information help future medical research of pHNP1-based native gene supply in tumor sufferers.

The downregulation of endogenous dhfr improved the effectivity of EBNA-1 amplification, as evidenced by a comparability with the amplification extent in cells missing shRNA expression on the similar MTX focus. The EBNA-1 expression ranges from the EBNA-1-amplified clones chosen on this research had been larger than these obtained from EBNA-1-amplified clones that had been generated utilizing the standard amplification in our earlier research. According to earlier research, EBNA-1 amplification improved the manufacturing of the Fc-fusion protein by a selected protein productiveness (qp)-enhancing impact, slightly than by bettering cell development or transfection effectivity.

Joint common modular plasmids (JUMP): a versatile vector platform for artificial biology

Technology of latest DNA constructs is an important course of in trendy life science and biotechnology. Modular cloning techniques based mostly on Golden Gate cloning, utilizing Kind IIS restriction endonucleases, permit meeting of complicated multipart constructs from reusable fundamental DNA components in a fast, dependable and automation-friendly manner. Many such toolkits can be found, with various levels of compatibility, most of that are aimed toward particular host organisms. Right here, we current a vector design which permits easy vector modification by utilizing modular cloning to assemble and add new capabilities in secondary websites flanking the primary insertion web site (used for typical modular cloning).

Preclinical safety evaluation of a recombinant plasmid vector encoding mature human neutrophil peptide-1 by repeated local administrations in nonhuman primates

Meeting in all websites is suitable with the PhytoBricks normal, and vectors are suitable with the Commonplace European Vector Structure (SEVA) in addition to BioBricks. We exhibit that this facilitates the development of vectors with tailor-made capabilities and simplifies the workflow for producing libraries of constructs with frequent components. We have now made out there a set of vectors with 10 totally different microbial replication origins, various in copy quantity and host vary, and permitting chromosomal integration, in addition to a choice of generally used fundamental components.

This design expands the vary of hosts which might be simply modified by modular cloning and acts as a toolkit which can be utilized to facilitate the technology of latest toolkits with particular capabilities required for focusing on additional hosts. The antigen produced by the described approach is appropriate for serological exams and subunit vaccine research. We developed a easy purification scheme that constantly yielded as much as 30 mg of RBD protein per liter of the easy shake flask cell tradition.

Excessive-level expression of the monomeric SARS-CoV-2 S protein RBD 320-537 in stably transfected CHO cells by the EEF1A1-based plasmid vector

The spike (S) protein is likely one of the three proteins forming the coronaviruses’ viral envelope. The S protein of the Extreme Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial construction much like the S proteins of different mammalian coronaviruses, aside from a singular receptor-binding area (RBD), which is a big inducer of host immune response. Recombinant SARS-CoV-2 RBD is broadly used as a extremely particular minimal antigen for serological exams.

Right publicity of antigenic determinants has a big affect on the accuracy of such tests-the antigen must be appropriately folded, comprise no probably antigenic non-vertebrate glycans, and, ideally, ought to have a glycosylation sample much like the native S protein. Based mostly on the beforehand developed p1.1 vector, containing the regulatory sequences of the Eukaryotic translation elongation issue 1 alpha gene (EEF1A1) from Chinese language hamster, we created two expression constructs encoding SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine tags.

pYLEX1 - Expression Vector

FYY203-5MG 5mg Ask for price

Blunt E1 Expression Kit

abx098098-10rxns 10 rxns
EUR 560.4

Blunt E2 Expression Kit

abx098873-10rxns 10 rxns
EUR 560.4

RFP expression Adenovirus

AVP001 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made RFP expression adenovirus, provided in DMEM medium.

CFP expression Adenovirus

AVP002 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made CFP expression adenovirus, provided in DMEM medium.

GFP expression Adenovirus

AVP011 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made GFP expression adenovirus, provided in DMEM medium.

YFP expression Adenovirus

AVP012 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made YFP expression adenovirus, provided in DMEM medium.

BFP expression Adenovirus

AVP017 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made BFP expression adenovirus, provided in DMEM medium.

h OCT4 expression Adenovirus, in vivo ready

AVP013-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made adenovirus express human stem cell factor, OCT4, provided in PBS solution for in vivo application.

h SOX2 expression Adenovirus, in vivo ready

AVP014-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made adenovirus express human stem cell factor, SOX2, provided in PBS solution for in vivo application.

h LIN28 expression Adenovirus, in vivo ready

AVP015-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made adenovirus express human stem cell factor, LIN28, provided in PBS solution for in vivo application.

Rac1 Expression Vector Set

STA-454 1 kit
EUR 1765.2
Description: Rac1 Expression Vector Set contains 3 vectors: Rac wild type, T17N dominant negative mutant, and G12V constitutively active mutant.

RhoA Expression Vector Set

STA-456 1 kit
EUR 1765.2
Description: RhoA Expression Vector Set contains 3 vectors: RhoA wild type, T19N dominant negative mutant, and G14V constitutively active mutant.

Cdc42 Expression Vector Set

STA-455 1 kit
EUR 1765.2
Description: Cdc42 Expression Vector Set contains 3 vectors: Cdc42 wild type, T17N dominant negative mutant, and Q61L constitutively active mutant.

h ADA Expression Lentivirus

LVP923 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: ADA, containing a RFP-Blasticidin dual selection marker.

h IL6 Expression Lentivirus

LVP1144 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h CD4 Expression Lentivirus

LVP1152 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: CD4, containing a RFP-Blasticidin dual selection marker.

h IL4 Expression Lentivirus

LVP1161 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: IL4, containing a RFP-Blasticidin dual selection marker.

h IL7 Expression Lentivirus

LVP1162 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: IL7, containing a RFP-Blasticidin dual selection marker.

h KDR Expression Lentivirus

LVP1176 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: KDR, containing a RFP-Blasticidin dual selection marker.

h FAS Expression Lentivirus

LVP1181 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: FAS, containing a RFP-Blasticidin dual selection marker.

pAAV-MCS Expression Vector

VPK-410 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

h OCT4 expression Adenovirus

AVP013 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made adenovirus express human stem cell factor, OCT4, provided in DMEM medium.

h SOX2 expression Adenovirus

AVP014 1x109 IFU/ml x 50ul
EUR 418.8
Description: pre-made adenovirus express human stem cell factor, SOX2, provided in DMEM medium.

h CDR1 Expression Lentivirus

LVP917 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: CDR1, containing a RFP-Blasticidin dual selection marker.

h CD47 Expression Lentivirus

LVP918 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: CD47, containing a RFP-Blasticidin dual selection marker.

h ESR1 Expression Lentivirus

LVP924 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: ESR1, containing a RFP-Blasticidin dual selection marker.

r Syn1 Expression Lentivirus

LVP925 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for rat target: Syn1, containing a RFP-Blasticidin dual selection marker.

h NME4 Expression Lentivirus

LVP932 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: NME4, containing a RFP-Blasticidin dual selection marker.

h EGFR Expression Lentivirus

LVP1142 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: EGFR, containing a RFP-Blasticidin dual selection marker.

h NRP1 Expression Lentivirus

LVP1145 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h MUC1 Expression Lentivirus

LVP1148 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h CD8a Expression Lentivirus

LVP1151 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: CD8a, containing a RFP-Blasticidin dual selection marker.

h PIGF Expression Lentivirus

LVP1154 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: PIGF, containing a RFP-Blasticidin dual selection marker.

h BRAF Expression Lentivirus

LVP1156 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: BRAF, containing a RFP-Blasticidin dual selection marker.

h ROS1 Expression Lentivirus

LVP1158 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: ROS1, containing a RFP-Blasticidin dual selection marker.

h CD3E Expression Lentivirus

LVP1163 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: IL7, containing a RFP-Blasticidin dual selection marker.

h KLK3 Expression Lentivirus

LVP1171 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: KLK3, containing a RFP-Blasticidin dual selection marker.

h NEK6 Expression Lentivirus

LVP1182 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: NEK6, containing a RFP-Blasticidin dual selection marker.

h NEK7 Expression Lentivirus

LVP1183 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: NEK7, containing a RFP-Blasticidin dual selection marker.

h LIN28 expression Adenovirus

AVP015 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made adenovirus express human stem cell factor, LIN28, provided in DMEM medium.

h GPNMB Expression Lentivirus

LVP921 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: GPNMB, containing a RFP-Blasticidin dual selection marker.

h DECR1 Expression Lentivirus

LVP927 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: DECR1, containing a RFP-Blasticidin dual selection marker.

h DECR2 Expression Lentivirus

LVP928 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: DECR2, containing a RFP-Blasticidin dual selection marker.

h NMRK1 Expression Lentivirus

LVP930 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: NMRK1, containing a RFP-Blasticidin dual selection marker.

h PITX3 Expression Lentivirus

LVP931 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: PITX3, containing a RFP-Blasticidin dual selection marker.

h HDAC1 Expression Lentivirus

LVP1143 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h VEGFB Expression Lentivirus

LVP1146 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h PARP1 Expression Lentivirus

LVP1149 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h IL3RA Expression Lentivirus

LVP1150 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: IL3RA, containing a RFP-Blasticidin dual selection marker.

h PTCH1 Expression Lentivirus

LVP1155 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: PTCH1, containing a RFP-Blasticidin dual selection marker.

h SART3 Expression Lentivirus

LVP1159 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: SART3, containing a RFP-Blasticidin dual selection marker.

h TIGIT Expression Lentivirus

LVP1160 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: TIGIT, containing a RFP-Blasticidin dual selection marker.

h EDNRA Expression Lentivirus

LVP1165 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: EDNRA containing a RFP-Blasticidin dual selection marker.

h Foxp3 Expression Lentivirus

LVP1172 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: Foxp3, containing a RFP-Blasticidin dual selection marker.

m RIKEN Expression Lentivirus

LVP1174 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for mouse target: RIKEN, containing a RFP-Blasticidin dual selection marker.

h APLP2 Expression Lentivirus

LVP1177 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: APLP2, containing a RFP-Blasticidin dual selection marker.

h PCSK9 Expression Lentivirus

LVP1178 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: PCSK9, containing a RFP-Blasticidin dual selection marker.

h NTSR1 Expression Lentivirus

LVP1179 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: NTSR1, containing a RFP-Blasticidin dual selection marker.

pscAAV-MCS Expression Vector

VPK-430 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

H-Ras Expression Vector Set

STA-457 1 kit
EUR 1765.2
Description: H-Ras Expression Vector Set contains 3 vectors: H-Ras wild type, T19N dominant negative mutant, and G12V constitutively active mutant.

m CTAGA1 Expression Lentivirus

LVP916 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for mouse target: CTAGA1, containing a RFP-Blasticidin dual selection marker.

h SKIV2L Expression Lentivirus

LVP919 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: SKIV2L, containing a RFP-Blasticidin dual selection marker.

h SKIV2L Expression Lentivirus

LVP919-GP 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: SKIV2L, containing a GFP-Puromycin dual selection marker.

h TP5313 Expression Lentivirus

LVP926 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: TP5313, containing a RFP-Blasticidin dual selection marker.

h ELOVL5 Expression Lentivirus

LVP929 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: ELOVL5, containing a RFP-Blasticidin dual selection marker.

h MAGEA3 Expression Lentivirus

LVP1147 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h NANOS3 Expression Lentivirus

LVP1164 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: NANOS3, containing a RFP-Blasticidin dual selection marker.

h IL1RL1 Expression Lentivirus

LVP1168 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: IL1RL1, containing a RFP-Blasticidin dual selection marker.

h CTAG1B Expression Lentivirus

LVP1169 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: CTAG1B, containing a RFP-Blasticidin dual selection marker.

h MAGEA3 Expression Lentivirus

LVP1170 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: MAGEA3, containing a RFP-Blasticidin dual selection marker.

h COX7A1 Expression Lentivirus

LVP1173 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: COX7A1, containing a RFP-Blasticidin dual selection marker.

h BCL11B Expression Lentivirus

LVP1180 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: BCL11B, containing a RFP-Blasticidin dual selection marker.

pUC57-sgRNA expression vector

PVT18003 2 ug
EUR 309.6

BRAF, His-Tag (High Expression)

41237 10 µg
EUR 375
Description: Human BRAF, also known as B-RAF1 and RAFB1, with 18 mutations to improve expression; S446A, S447A, I543A, I544S, I551K, Q562R, L588N, K630S, F667E, Y672S, A688R, L706S, Q709R, S713E, L716E, S720E, P722S, K723G. GenBank Accession #NM_004333, a.a. 444-723 with N-terminal His-tag, expressed in E. coli cells. MW = 33 kDa.

ANPRA/Aequorin Expression vector

60641 20 µg
EUR 900
Description: The ANPRA/Aequorin expression vector is designed to co-express human atrial natriuretic peptide receptor A (ANPRA, also called natriuretic peptide receptor A/guanylate cyclase A) and jellyfish (Aequorea victoria) Aequorin in mammalian cells.

h CEACAM5 Expression Lentivirus

LVP1153 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: CEACAM5, containing a RFP-Blasticidin dual selection marker.

h TNFSF11 Expression Lentivirus

LVP1157 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: TNFSF11, containing a RFP-Blasticidin dual selection marker.

h TNFRSF1B Expression Lentivirus

LVP922 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: TNFRSF1B, containing a RFP-Blasticidin dual selection marker.

h KIAA1324 Expression Lentivirus

LVP1175 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: KIAA1324, containing a RFP-Blasticidin dual selection marker.

GFP-Rac1 Expression Vector Set

STA-450 1 kit
EUR 1939.2
Description: GFP-Rac1 Expression Vector Set contains 3 vectors: Rac wild type, T17N dominant negative mutant, and Q61L constitutively active mutant. Each vector also contains a GFP reporter sequence.

GFP-RhoA Expression Vector Set

STA-452 1 kit
EUR 1939.2
Description: GFP-RhoA Expression Vector Set contains 3 vectors: RhoA wild type, T19N dominant negative mutant, and Q63L constitutively active mutant. Each vector also contains a GFP reporter sequence.

h KIAA0319L Expression Lentivirus

LVP920 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: KIAA0319L, containing a RFP-Blasticidin dual selection marker.

GFP-Cdc42 Expression Vector Set

STA-451 1 kit
EUR 1939.2
Description: GFP-Cdc42 Expression Vector Set contains 3 vectors: Cdc42 wild type, T17N dominant negative mutant, and Q61L constitutively active mutant. Each vector also contains a GFP reporter sequence.

Active Rac1 Expression Vector Set

STA-458 1 kit
EUR 1765.2
Description: Active Rac1 Expression Vector Set contains 3 vectors expressing different constitutively active mutants of Rac1: Q61L, Q61L/F37A, and Q61L/Y40C.

PhiC31 Integrase Expression Plasmid

FC200PA-1 10ug
EUR 496

pCMV-GFP-LC3 Expression Vector

CBA-401 100 µL
EUR 1243.2
Description: Expression vector contains a fusion of GFP and LC3. A separate GFP control vector is also included.

h KRAS (G12D) Expression Lentivirus

LVP1166 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for a mutant (G12D) of human target: KRAS containing a RFP-Blasticidin dual selection marker.

Tet-on circRNA Expression Vector

PVT14643 2 ug
EUR 843.6

h TP53 (R175H) Expression Lentivirus

LVP1167 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for a mutant R175H) of human target: TP53, containing a RFP-Blasticidin dual selection marker.

pAAV-IRES-Neo Expression Vector

VPK-416 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-GFP Expression Vector

VPK-418 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-Bsd Expression Vector

VPK-419 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

PinPoint Integrase Expression Plasmid

PIN200A-1 10ug
EUR 688

pAAV-IRES-Puro Expression Vector

VPK-415 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

CEACAM5 CHO Cell Line (Low Expression)

78704-L 2 vials
EUR 7150
Description: Recombinant clonal CHO cell line stably expressing full-length human CEACAM5 (NM_004363). Surface expression of CEACAM5 was confirmed by flow cytometry. The stable clonal cell line was selected for different levels of CEACAM5 expression (High, Medium, and Low) compared to the parental CHO-K1 cell line.

Active H-Ras Expression Vector Set

STA-459 1 kit
EUR 1765.2
Description: Active H-Ras Expression Vector Set contains 3 vectors expressing different constitutively active mutants of H-Ras: V12, V12S35, and V12C40.

B7-H3 CHO Cell Line (Low Expression)

78541-L 2 vials
EUR 7150
Description: B7 homolog 3 protein (B7-H3) CHO Cell line is a recombinant clonal stable CHO cell line expressing full length human B7-H3 protein, also known as cluster of differentiation 276 (CD276, B7H3; B7RP-2; 4Ig-B7-H3). Surface expression of B7-H3 was confirmed by flow cytometry. Each stable clonal cell line was selected for High, Medium, or Low levels of B7-H3 expression to mimic different B7-H3 expression levels in various cancer types.

pAAV-IRES-Hygro Expression Vector

VPK-417 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

CD36 HEK293 Cell Line (High Expression)

78526-H 2 vials
EUR 7150
Description: Recombinant clonal HEK293 cell line stably expressing full length human CD36. The stable clonal cell line was selected for medium and high levels of CD36 expression compared to the parental HEK293 cell line.

CEACAM5 CHO Cell Line (High Expression)

78704-H 2 vials
EUR 7150
Description: Recombinant clonal CHO cell line stably expressing full-length human CEACAM5 (NM_004363). Surface expression of CEACAM5 was confirmed by flow cytometry. The stable clonal cell line was selected for different levels of CEACAM5 expression (High, Medium, and Low) compared to the parental CHO-K1 cell line.

AAV-1 Helper Free Expression System

VPK-410-SER1 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-2 Helper Free Expression System

VPK-410-SER2 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-3 Helper Free Expression System

VPK-410-SER3 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-4 Helper Free Expression System

VPK-410-SER4 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-5 Helper Free Expression System

VPK-410-SER5 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-6 Helper Free Expression System

VPK-410-SER6 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

B7-H3 CHO Cell Line (High Expression)

78541-H 2 vials
EUR 7150
Description: B7 homolog 3 protein (B7-H3) CHO Cell line is a recombinant clonal stable CHO cell line expressing full length human B7-H3 protein, also known as cluster of differentiation 276 (CD276, B7H3; B7RP-2; 4Ig-B7-H3). Surface expression of B7-H3 was confirmed by flow cytometry. Each stable clonal cell line was selected for High, Medium, or Low levels of B7-H3 expression to mimic different B7-H3 expression levels in various cancer types.

AAV-DJ Helper Free Expression System

VPK-410-DJ 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2.

Gryphon Retroviral Expression System I

ABP-RVK-10001 1 set Ask for price
Description: PRODUCT LINE DISCONTINUED. Please contact us.

Gryphon Retroviral Expression System II

ABP-RVK-10002 1 set Ask for price
Description: PRODUCT LINE DISCONTINUED. Please contact us.

CD7 CHO Cell Line (Medium or High Expression)-78324-H

78324-H 2 vials
EUR 7150
Description: Recombinant CD7 CHO-K1 cell lines expressing full-length human CD7 receptor (accession number: NM_006137.6). Surface expression of human CD7 was confirmed by flow cytometry. Each stable clonal cell line was selected for high or medium levels of CD7 expression to mimic different stages of cancer target cells with various CD7 expression levels.

CD7 CHO Cell Line (Medium or High Expression)-78324-M

78324-M 2 vials
EUR 7150
Description: Recombinant CD7 CHO-K1 cell lines expressing full-length human CD7 receptor (accession number: NM_006137.6). Surface expression of human CD7 was confirmed by flow cytometry. Each stable clonal cell line was selected for high or medium levels of CD7 expression to mimic different stages of cancer target cells with various CD7 expression levels.

CD36 HEK293 Cell Line (Medium Expression)

78526-M 2 vials
EUR 7150
Description: Recombinant clonal HEK293 cell line stably expressing full length human CD36. The stable clonal cell line was selected for medium and high levels of CD36 expression compared to the parental HEK293 cell line.

CEACAM5 CHO Cell Line (Medium Expression)

78704-M 2 vials
EUR 7150
Description: Recombinant clonal CHO cell line stably expressing full-length human CEACAM5 (NM_004363). Surface expression of CEACAM5 was confirmed by flow cytometry. The stable clonal cell line was selected for different levels of CEACAM5 expression (High, Medium, and Low) compared to the parental CHO-K1 cell line.

Human Myelin expression factor 2 (MYEF2)

1-CSB-EP015283HU
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Myelin expression factor 2(MYEF2),partial expressed in E.coli

scAAV-1 Helper Free Expression System

VPK-430-SER1 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

scAAV-2 Helper Free Expression System

VPK-430-SER2 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

scAAV-3 Helper Free Expression System

VPK-430-SER3 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

scAAV-4 Helper Free Expression System

VPK-430-SER4 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

scAAV-5 Helper Free Expression System

VPK-430-SER5 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

scAAV-6 Helper Free Expression System

VPK-430-SER6 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

B7-H3 CHO Cell Line (Medium Expression)

78541-M 2 vials
EUR 7150
Description: B7 homolog 3 protein (B7-H3) CHO Cell line is a recombinant clonal stable CHO cell line expressing full length human B7-H3 protein, also known as cluster of differentiation 276 (CD276, B7H3; B7RP-2; 4Ig-B7-H3). Surface expression of B7-H3 was confirmed by flow cytometry. Each stable clonal cell line was selected for High, Medium, or Low levels of B7-H3 expression to mimic different B7-H3 expression levels in various cancer types.

CD95 CHO Cell Line (Medium or High Expression)-78499-H

78499-H 2 vials
EUR 7150
Description: The CD95 CHO cell line is a recombinant clonal stable CHO cell line constitutively expressing full-length human CD95 receptor (accession number: NM_000043.3). Surface expression of human CD95 was confirmed by flow cytometry. Each clonal cell line was selected for different levels of CD95 expression (High or Medium) to mimic varying CD95 expression levels in cancer cells.

CD95 CHO Cell Line (Medium or High Expression)-78499-M

78499-M 2 vials
EUR 7150
Description: The CD95 CHO cell line is a recombinant clonal stable CHO cell line constitutively expressing full-length human CD95 receptor (accession number: NM_000043.3). Surface expression of human CD95 was confirmed by flow cytometry. Each clonal cell line was selected for different levels of CD95 expression (High or Medium) to mimic varying CD95 expression levels in cancer cells.

Protein Limb Expression 1 (LIX1) Antibody

20-abx318393
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Protein Limb Expression 1 (LIX1) Antibody

abx146234-100ug 100 ug
EUR 469.2

Protein Limb Expression 1 (LIX1) Antibody

abx025913-400ul 400 ul
EUR 627.6

Protein Limb Expression 1 (LIX1) Antibody

abx025913-80l 80 µl
EUR 343.2

scAAV-DJ Helper Free Expression System

VPK-430-DJ 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2.

pRedPuro shRNA expression lentivector (EF1)

SI516A-1 10 ug
EUR 594

pAAV-MCS Promoterless Expression Vector

VPK-411 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pSMPUW-Neo Lentiviral Expression Vector

VPK-213 10 µg
EUR 748.8
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.

Claudin-6 CHO Cell Line (Low Expression)

78527-L 2 vials
EUR 7150
Description: Recombinant clonal CHO cell line stably expressing full-length human claudin-6 (CLDN6 gene accession number NM_021195.5).  Surface expression of claudin 6 was confirmed by flow cytometry. The stable clonal cell line was selected for high, medium, or low levels of claudin-6 expression compared to the parental CHO-K1 cell line.

RAPAd® RSV Adenoviral Expression System

VPK-251 1 kit
EUR 1243.2
Description: Making an adenovirus with traditional recombinant methods takes 2-3 months and requires tedious plaque recombination. More recent technologies have shortened this time somewhat, but still produce relatively high amounts of wild type (replication-competent) plaques, levels of which increase with serial amplification. The RAPAd® Adenoviral Expression Systems produce recombinant adenovirus with substantially reduced wild-type virus, while considerably shortening the production time to 2-3 weeks. Serial amplification of adenovirus produced using the RAPAd® system does not significantly increase replication-competent adenovirus levels. The system uses a backbone vector from which the 5' ITR, packaging signal and E1 sequences have been removed.

RAPAd® CMV Adenoviral Expression System

VPK-252 1 kit
EUR 1243.2
Description: Making an adenovirus with traditional recombinant methods takes 2-3 months and requires tedious plaque recombination. More recent technologies have shortened this time somewhat, but still produce relatively high amounts of wild type (replication-competent) plaques, levels of which increase with serial amplification. The RAPAd® Adenoviral Expression Systems produce recombinant adenovirus with substantially reduced wild-type virus, while considerably shortening the production time to 2-3 weeks. Serial amplification of adenovirus produced using the RAPAd® system does not significantly increase replication-competent adenovirus levels. The system uses a backbone vector from which the 5' ITR, packaging signal and E1 sequences have been removed.

pSMPUW-Puro Lentiviral Expression Vector

VPK-212 10 µg
EUR 748.8
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.

Claudin-6 CHO Cell Line (High Expression)

78527-H 2 vials
EUR 7150
Description: Recombinant clonal CHO cell line stably expressing full-length human claudin-6 (CLDN6 gene accession number NM_021195.5).  Surface expression of claudin 6 was confirmed by flow cytometry. The stable clonal cell line was selected for high, medium, or low levels of claudin-6 expression compared to the parental CHO-K1 cell line.

AAV-DJ/8 Helper Free Expression System

VPK-410-DJ-8 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2. AAV-DJ/8 was created by making a point mutation in the heparin binding domain of AAV-DJ. Recombinant AAV produced from the AAV-DJ/8 system closely resembles AAV-8 and AAV-9 in its ability to infect heart and brain tissues.

Myelin Expression Factor 2 (MYEF2) Antibody

abx235464-100ug 100 ug
EUR 577.2

Myelin Expression Factor 2 (MYEF2) Antibody

20-abx109333
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Myelin Expression Factor 2 (MYEF2) Antibody

20-abx113906
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Expression Negative Control Lentivirus (G418)

79902-G 500 µl x 2
EUR 795
Description: The Expression Negative Control Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The controls package the same virus particles as the target expression virus, but they do not express a specific protein under the CMV promoter. _x000D_The Expression Negative Control Lentivirus (G418) expresses the gene for aminoglycoside 3' phosphotransferase, which confers resistance to kanamycin, neomycin, and geneticin (G418)._x000D_The Expression Negative Control Lentivirus (Hygromycin) expresses the gene for hygromycin B phosphotransferase, which confers resistance to Hygromycin._x000D_The Expression Negative Control Lentivirus (Puromycin) expresses the gene for puromycin N-acetyl-transferase, which confers resistance to puromycin._x000D_ _x000D_

pGreenPuro shRNA expression lentivector (CMV)

SI505A-1 10 ug
EUR 594

pGreenPuro shRNA expression lentivector (EF1)

SI506A-1 10 ug
EUR 594

pSMPUW-Hygro Lentiviral Expression Vector

VPK-214 10 µg
EUR 748.8
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.

Baculovirus expression vector pAc-g-CH3

30R-AB001 5 ug
EUR 739.2
Description: Baculovirus cassette vector pAc-l-CH3 for the expression of human, humanized or chimeric IgG(lambda) in insect cells and secretion of assembled antibodies into the supernatant.

Genorise® Chicken CCL7 Expression Plasmid

GR148007 10 µg
EUR 219

RAPAd® miRNA Adenoviral Expression System

VPK-253 1 kit
EUR 1243.2
Description: Making an adenovirus with traditional recombinant methods takes 2-3 months and requires tedious plaque recombination. More recent technologies have shortened this time somewhat, but still produce relatively high amounts of wild type (replication-competent) plaques, levels of which increase with serial amplification. The RAPAd® Adenoviral Expression Systems produce recombinant adenovirus with substantially reduced wild-type virus, while considerably shortening the production time to 2-3 weeks. Serial amplification of adenovirus produced using the RAPAd® system does not significantly increase replication-competent adenovirus levels. The system uses a backbone vector from which the 5' ITR, packaging signal and E1 sequences have been removed.

Claudin-6 CHO Cell Line (Medium Expression)

78527-M 2 vials
EUR 7150
Description: Recombinant clonal CHO cell line stably expressing full-length human claudin-6 (CLDN6 gene accession number NM_021195.5).  Surface expression of claudin 6 was confirmed by flow cytometry. The stable clonal cell line was selected for high, medium, or low levels of claudin-6 expression compared to the parental CHO-K1 cell line.

scAAV-DJ/8 Helper Free Expression System

VPK-430-DJ-8 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2. AAV-DJ/8 was created by making a point mutation in the heparin binding domain of AAV-DJ. Recombinant AAV produced from the AAV-DJ/8 system closely resembles AAV-8 and AAV-9 in its ability to infect heart and brain tissues.

pC001 - huLshC2C2- MBP for bacterial expression

PVT10645 2 ug
EUR 361.2

Exoenzyme C3 (Rho Inhibitor) Expression Vector

STA-460 1 kit
EUR 860.4
Description: This mammalian expression vector codes for Exoenzyme C3, a Rho inhibitor.

Protein Limb Expression 1 (LIX1) Antibody (HRP)

20-abx308975
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

LEXSYcon2.1 Expression Kit M-EGE-1310ble ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight

M-EGE-1310ble 1 Kit
EUR 1682
Description: LEXSYcon2.1 Expression Kit M-EGE-1310ble ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight

LEXSYcon2.1 Expression Kit M-EGE-1310bsd ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight

M-EGE-1310bsd 1 Kit
EUR 1682
Description: LEXSYcon2.1 Expression Kit M-EGE-1310bsd ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight

LEXSYcon2.1 Expression Kit M-EGE-1310hyg ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight

M-EGE-1310hyg 1 Kit
EUR 1682
Description: LEXSYcon2.1 Expression Kit M-EGE-1310hyg ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight

LEXSYcon2.1 Expression Kit M-EGE-1310neo ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight

M-EGE-1310neo 1 Kit
EUR 1682
Description: LEXSYcon2.1 Expression Kit M-EGE-1310neo ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight

LEXSYcon2.1 Expression Kit M-EGE-1310pac ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight

M-EGE-1310pac 1 Kit
EUR 1682
Description: LEXSYcon2.1 Expression Kit M-EGE-1310pac ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight

LEXSYcon2.1 Expression Kit M-EGE-1310sat ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight

M-EGE-1310sat 1 Kit
EUR 1682
Description: LEXSYcon2.1 Expression Kit M-EGE-1310sat ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight

Cre Recombinase Expression Plasmid (pCMV-CRE)

CRE100A-1 10 µg
EUR 412

Flp recombinase expression HEK293 stable cell

SC006 2 x 106 cell/ml x 1ml
EUR 2670
Description: Flp recombinase stable cell line with Blasticidin resistance

Recombinant DNA expression in Pichia pastoris

HESAP-1002-10G 10G
EUR 2250

BCMA CHO Recombinant Cell Line (Low Expression)

79500-L 2 vials
EUR 6500
Description: Recombinant clonal stable CHO cell line constitutively expressing full length human BCMA protein, also known as TNFRSF17, (Genbank #NM_001192). Surface expression of BCMA was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of BCMA expression, (H. M, L), to mimic different stages of cancer target cells with various BCMA expression levels.

CD19 CHO Recombinant Cell Line (Low Expression)

79561-L 2 vials
EUR 7150
Description: Recombinant clonal stable CHO cell line constitutively expressing full length human CD19 protein, also known as B4 or CVID3, (Genbank #NM_001770). Surface expression of CD19 was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of CD19 expression (High, Medium, Low) to mimic different stages of cancer target cells with various CD19 expression levels.

CD37/CHO Recombinant Cell Line (Low Expression)

79607-L 2 vials
EUR 6500
Description: Recombinant clonal stable CHO cell line constitutively expressing full length human CD37 protein, also known as GP52-40; TSPAN26; leukocyte antigen CD37 (Genbank #NM_001774). Surface expression of CD37 was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of CD37 expression, (High, Medium, Low), to mimic different stages of cancer target cells with various CD37 expression levels.

RBDv1 contained a local viral sign peptide, RBDv2 -human tPA sign peptide. We transfected a CHO DG44 cell line, chosen stably transfected cells, and carried out just a few rounds of methotrexate-driven amplification of the genetic cassette within the genome. For the RBDv2 variant, a high-yield clonal producer cell line was obtained. Purified proteins had been analyzed by polyacrylamide gel electrophoresis in lowering and non-reducing situations and gel filtration; for RBDv2 protein, the monomeric type content material exceeded 90% for a number of sequence. Deglycosylation with PNGase F and mass spectrometry confirmed the presence of N-glycosylation.

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