Right here, we explored whether or not a minicircle DNA (MC) vector containing human angiopoietin 1 (MC-ANGPT1) can present a more practical platform for gene-enhanced MSC remedy of ALI/ARDS. At 24 h after transfection, nuclear-targeted electroporation utilizing an MC-ANGPT1 vector resulted in a 3.7-fold better enhance in human ANGPT1 protein in MSC conditioned media in comparison with the usage of a plasmid ANGPT1 vector. Within the lipopolysaccharide (LPS)-induced ALI mannequin, administration of pANGPT1 transfected MSCs considerably decreased bronchoalveolar lavage (BAL) neutrophil counts by 57%, whereas MC-ANGPT1 transfected MSCs decreased it by 71% (p < 0.001) by Holm-Sidak’s a number of comparability take a look at.
Furthermore, in comparison with pANGPT1, the MC-ANGPT1 transfected MSCs considerably decreased pulmonary irritation, as noticed in decreased ranges of proinflammatory cytokines, akin to tumor necrosis factor-alpha (TNF-α), interferon-gamma (IFN-γ), interleukin-6 (IL-6), monocyte chemoattractant protein-1 (MCP-1), and macrophage inflammatory protein-2 (MIP-2). pANGPT1-transfected MSCs considerably decreased BAL albumin ranges by 71%, whereas MC-ANGPT1-transfected MSCs decreased it by 85%. Total, utilizing a minicircle vector, we demonstrated an environment friendly and sustained expression of the ANGPT1 transgene in MSCs and enhanced the therapeutic impact on the ALI mannequin in comparison with plasmid.
Plasmid Vectors for in Vivo Choice-Free Use with the Probiotic E. coli Nissle 1917
Escherichia coli Nissle 1917 (EcN) is a probiotic bacterium, generally employed to deal with sure gastrointestinal issues. It’s quick rising as an essential goal for the event of therapeutic engineered micro organism, benefiting from the wealth of information of E. coli biology and ease of manipulation. Bacterial artificial biology initiatives generally make the most of engineered plasmid vectors, that are easy to engineer and might reliably obtain excessive ranges of protein expression. Nevertheless, plasmids usually require antibiotics for upkeep, and the administration of an antibiotic is commonly incompatible with in vivo experimentation or remedy.
EcN natively accommodates plasmids pMUT1 and pMUT2, which don’t have any recognized operate however are secure inside the micro organism. Right here, we describe the event of the pMUT plasmids into a strong platform for engineering EcN for in vivo experimentation, alongside a CRISPR-Cas9 system to take away the native plasmids. We systematically engineered each pMUT plasmids to include choice markers, fluorescent markers, temperature delicate expression, and curli secretion programs to export a customizable purposeful materials into the extracellular area.
We then reveal that the engineered plasmids had been maintained in micro organism because the engineered micro organism go by the mouse GI tract with out choice, and that the secretion system stays purposeful, exporting functionalized curli proteins into the intestine. Our plasmid system presents a platform for the fast improvement of therapeutic EcN micro organism.
Cloning in Plasmid Vectors: Blunt-Finish Cloning
This protocol describes procedures for cloning blunt-ended DNA fragments into linearized plasmid vectors. To acquire the utmost variety of “appropriate” ligation merchandise when cloning blunt-ended goal fragments, the 2 elements of DNA within the ligation response have to be current at an acceptable ratio. These outcomes assist the potential advantages of MC-ANGPT1 gene enhancement of MSC remedy to deal with ARDS.
If the molar ratio of plasmid vector to focus on DNA is just too excessive, then the ligation response might generate an undesirable variety of round empty plasmids, each monomeric and polymeric; if too low, the ligation response might generate an extra of linear and round homopolymers and heteropolymers of various sizes, orientations, and compositions. Because of this, the orientation of the overseas DNA and the variety of inserts in every recombinant clone should at all times be validated by restriction endonuclease mapping or another means.
Plasmid copy quantity variation of a modular vector set in Shewanella oneidensis MR-1
To advance artificial biology approaches that make the most of S. oneidensis as host for biotechnology functions, now we have investigated the variation in plasmid copy variety of a modular vector set ensuing from distinct origins of replication beneath totally different circumstances. The replicons yielded a ≈9X-fold vary for plasmid copy quantity variation in S. oneidensis (whereas the identical origins yielded a ≈3X-fold vary in Escherichia coli). This offers a sizeable vary to regulate gene expression ranges in S. oneidensis for artificial biology functions. As well as, plasmid harboring the pBBR1 origin resulted in secure copy numbers in S. oneidensis beneath totally different circumstances (mid-logarithmic, stationary, multi-plasmid).
anti- NFKB2 antibody |
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FNab05705 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against NFKB2 |
anti- NFKB2 antibody |
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FNab05706 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against NFKB2 |
Rat Transcription factor p65 / NFKB3 (RELA) ELISA Kit |
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abx255851-96tests | Abbexa | 96 tests | EUR 801.6 |
Pig Transcription factor p65 / NFKB3 (RELA) ELISA Kit |
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abx361108-96tests | Abbexa | 96 tests | EUR 990 |
Rat Transcription factor p65 / NFKB3 (RELA) ELISA Kit |
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20-abx156040 | Abbexa |
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Mouse Transcription factor p65 / NFKB3 (RELA) ELISA Kit |
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abx254289-96tests | Abbexa | 96 tests | EUR 801.6 |
Human Transcription factor p65 / NFKB3 (RELA) ELISA Kit |
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abx252815-96tests | Abbexa | 96 tests | EUR 801.6 |
Sheep Transcription factor p65 / NFKB3 (RELA) ELISA Kit |
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abx364265-96tests | Abbexa | 96 tests | EUR 1111.2 |
Mouse Transcription factor p65 / NFKB3 (RELA) ELISA Kit |
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abx570127-96tests | Abbexa | 96 tests | EUR 801.6 |
Human Transcription factor p65 / NFKB3 (RELA) ELISA Kit |
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abx570125-96tests | Abbexa | 96 tests | EUR 801.6 |
Human Transcription factor p65 / NFKB3 (RELA) ELISA Kit |
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20-abx152945 | Abbexa |
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Rabbit Transcription factor p65 / NFKB3 (RELA) ELISA Kit |
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abx362582-96tests | Abbexa | 96 tests | EUR 990 |
Monkey Transcription factor p65 / NFKB3 (RELA) ELISA Kit |
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abx359107-96tests | Abbexa | 96 tests | EUR 990 |
Chicken Transcription factor p65 / NFKB3 (RELA) ELISA Kit |
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abx357192-96tests | Abbexa | 96 tests | EUR 990 |
Anti-NFkB p105/p50/NFKB1 Antibody |
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PB9149 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-NFkB p100/p52/NFKB2 Antibody |
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PB9150 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-NFkB p100/p52/NFKB2 Antibody |
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PA1946 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-NFkB p100/p52/NFKB2 Antibody |
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PA1946-1 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-NFkB p105/p50/NFKB1 Antibody |
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PA2111 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-NFkB p105/p50/NFKB1 Antibody |
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PA2111-1 | BosterBio | 100ug/vial | EUR 352.8 |
Guinea pig Transcription factor p65 / NFKB3 (RELA) ELISA Kit |
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abx357262-96tests | Abbexa | 96 tests | EUR 990 |
anti- NFKBIE antibody |
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FNab05708 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against NFKBIE |
anti- NFKBIZ antibody |
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FNab05709 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against NFKBIZ |
anti- NFKB1,p105,p50-Specific antibody |
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FNab05704 | FN Test | 100µg | EUR 702 |
Description: Antibody raised against NFKB1,p105,p50-Specific |
anti- NFKB2,p52,p100-Specific antibody |
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FNab05707 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against NFKB2,p52,p100-Specific |
Rabbit Anti-Human NFKB1 (aa 930-934) Polyclonal Antibody |
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CPB-1129RH | Creative Diagnostics | 100 ul | EUR 552 |
Rabbit Anti-Human NFKB1 (aa 160-170) Polyclonal Antibody |
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CPB-1169RH | Creative Diagnostics | 100 ul | EUR 552 |
Rabbit Anti-Human NFKB2 (aa 160-171) Polyclonal Antibody |
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CPB-1176RH | Creative Diagnostics | 100 ul | EUR 552 |
Rabbit Anti-Human NFKB2 Polyclonal Antibody, aa.864-868 |
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CPB-930RH | Creative Diagnostics | 100 ul | EUR 552 |
Rabbit Anti-Human NFKB2 Polyclonal Antibody, aa.868-872 |
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CPB-931RH | Creative Diagnostics | 100 ul | EUR 552 |
Rabbit Anti-Human NFKB1 Polyclonal Antibody, aa.335-339 |
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CPB-932RH | Creative Diagnostics | 100 ul | EUR 552 |
Rabbit Anti-Human NFKB1 Polyclonal Antibody, aa.891-895 |
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CPB-933RH | Creative Diagnostics | 100 ul | EUR 552 |
Rabbit Anti-Human NFKB1 Polyclonal Antibody, aa.905-909 |
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CPB-934RH | Creative Diagnostics | 100 ul | EUR 552 |
Anti-IKB beta/NFKBIB Antibody |
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PB9292 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-IKB alpha/NFKBIA Antibody |
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PB9291 | BosterBio | 100ug/vial | EUR 400.8 |
NFKB1 antibody |
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10R-11003 | Fitzgerald | 100 ug | EUR 418.8 |
Description: Mouse monoclonal NFKB1 antibody |
NFKB1 antibody |
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10R-11155 | Fitzgerald | 100 ug | EUR 418.8 |
Description: Mouse Monoclonal NFKB1 antibody |
NFKB2 antibody |
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10R-1605 | Fitzgerald | 100 ug | EUR 614.4 |
Description: Mouse monoclonal NFKB2 antibody |
NFKB1 antibody |
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20-PR03 | Fitzgerald | 100 ul | EUR 682.8 |
Description: Rabbit polyclonal NFKB1 antibody |
NFKB2 antibody |
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38583-100ul | SAB | 100ul | EUR 302.4 |
NFKB2 Antibody |
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37182-100ul | SAB | 100ul | EUR 302.4 |
NFKB1 antibody |
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39087-100ul | SAB | 100ul | EUR 302.4 |
NFKB1 Antibody |
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BF0466 | Affbiotech | 200ul | EUR 540 |
NFKB1 antibody |
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70R-10525 | Fitzgerald | 50 ug | EUR 560.4 |
Description: Affinity purified rabbit polyclonal NFKB1 antibody |
NFKB1 antibody |
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70R-18860 | Fitzgerald | 50 ul | EUR 522 |
Description: Rabbit polyclonal NFKB1 antibody |
NFKB2 antibody |
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70R-18861 | Fitzgerald | 50 ul | EUR 522 |
Description: Rabbit polyclonal NFKB2 antibody |
NFKB1 antibody |
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70R-15433 | Fitzgerald | 100 ug | EUR 392.4 |
Description: Rabbit polyclonal NFKB1 antibody |
NFKB1 Antibody |
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35588-100ul | SAB | 100ul | EUR 302.4 |
NFKB2 Antibody |
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ABD7190 | Lifescience Market | 100 ug | EUR 525.6 |
NFKB2 Antibody |
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1-CSB-PA003428 | Cusabio |
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Description: A polyclonal antibody against NFKB2. Recognizes NFKB2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, IP, IF, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IP:2-5ug/mglysate.IF:1/200-1/1000.ELISA:1/20000 |
NFKB2 Antibody |
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1-CSB-PA003429 | Cusabio |
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Description: A polyclonal antibody against NFKB2. Recognizes NFKB2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/10000 |
NFKB1 Antibody |
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1-CSB-PA003430 | Cusabio |
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Description: A polyclonal antibody against NFKB1. Recognizes NFKB1 from Human. This antibody is Unconjugated. Tested in the following application: WB, IHC, IP, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IP:2-5ug/mglysate.ELISA:1/10000 |
NFKB1 Antibody |
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1-CSB-PA003431 | Cusabio |
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Description: A polyclonal antibody against NFKB1. Recognizes NFKB1 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: WB, IHC, IF, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/40000 |
NFKB1 Antibody |
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1-CSB-PA003434 | Cusabio |
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Description: A polyclonal antibody against NFKB1. Recognizes NFKB1 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: WB, IHC, IF, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/40000 |
NFKB1 Antibody |
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1-CSB-PA004763 | Cusabio |
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Description: A polyclonal antibody against NFKB1. Recognizes NFKB1 from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000 |
NFKB1 Antibody |
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1-CSB-PA10354A0Rb | Cusabio |
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Description: A polyclonal antibody against NFKB1. Recognizes NFKB1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF, ChIP; Recommended dilution: WB:1:500-1:5000, IHC:1:100-1:500, IF:1:50-1:500 |
NFKB2 Antibody |
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1-CSB-PA10449A0Rb | Cusabio |
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Description: A polyclonal antibody against NFKB2. Recognizes NFKB2 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200 |
NFKB1 Antibody |
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1-CSB-PA259078 | Cusabio |
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Description: A polyclonal antibody against NFKB1. Recognizes NFKB1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:500-1:5000, IHC:1:5-1:20 |
NFKB2 Antibody |
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1-CSB-PA821156 | Cusabio |
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Description: A polyclonal antibody against NFKB2. Recognizes NFKB2 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:2000-1:10000, WB:1:1000-1:5000 |
NFKB2 Antibody |
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1-CSB-PA785439 | Cusabio |
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Description: A polyclonal antibody against NFKB2. Recognizes NFKB2 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:2000, IHC:1:15-1:50 |
NFKB2 Antibody |
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1-CSB-PA015760GA01HU | Cusabio |
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Description: A polyclonal antibody against NFKB2. Recognizes NFKB2 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB |
NFKB1 Antibody |
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1-CSB-PA020085 | Cusabio |
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Description: A polyclonal antibody against NFKB1. Recognizes NFKB1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/20000 |
NFKB1 Antibody |
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1-CSB-PA020086 | Cusabio |
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Description: A polyclonal antibody against NFKB1. Recognizes NFKB1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC:1/100-1/300.ELISA:1/5000 |
NFKB1 Antibody |
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1-CSB-PA020090 | Cusabio |
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Description: A polyclonal antibody against NFKB1. Recognizes NFKB1 from Human. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/20000 |
NFKB1 Antibody |
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1-CSB-PA020096 | Cusabio |
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Description: A polyclonal antibody against NFKB1. Recognizes NFKB1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/20000 |
NFKB1 Antibody |
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1-CSB-PA015759GA01HU | Cusabio |
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Description: A polyclonal antibody against NFKB1. Recognizes NFKB1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB |
NFKB1 Antibody |
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1-CSB-PA441503 | Cusabio |
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Description: A polyclonal antibody against NFKB1. Recognizes NFKB1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:1000-1:2000, WB:1:200-1:1000 |
NFKB1 Antibody |
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1-CSB-PA546304 | Cusabio |
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Description: A polyclonal antibody against NFKB1. Recognizes NFKB1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:500-1:5000, IHC:1:25-1:100 |
NFKB1 Antibody |
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F52441-0.4ML | NSJ Bioreagents | 0.4 ml | EUR 330.65 |
Description: NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NFkB is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NFkB complexes are held in the cytoplasm in an inactive state complexed with members of the NFkB inhibitor (I-kappa-B) family. In a conventional activation pathway, IkB is phosphorylated by IkB kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NFkB complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NFkB p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NFkB proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105. |
NFKB1 Antibody |
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F54028-0.1ML | NSJ Bioreagents | 0.1 ml | EUR 322.15 |
Description: NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. [UniProt] |
NFKB1 Antibody |
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F47079-0.4ML | NSJ Bioreagents | 0.4 ml | EUR 322.15 |
Description: This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene. |
NFKB2 Antibody |
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F47541-0.4ML | NSJ Bioreagents | 0.4 ml | EUR 322.15 |
Description: NF-kappa-B has been detected in numerous cell types that express cytokines, chemokines, growth factors, cell adhesion molecules, and some acute phase proteins in health and in various disease states. NF-kappa-B is activated by a wide variety of stimuli, such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. Inappropriate activation of NF-kappa-B has been linked to inflammatory events associated with autoimmune arthritis, asthma, septic shock, lung fibrosis, glomerulonephritis, atherosclerosis, and AIDS. In contrast, complete and persistent inhibition of NF-kappa-B has been linked directly to apoptosis, inappropriate immune cell development, and delayed cell growth. NFKB1 (MIM 164011) and NFKB2 encode p105 and p100 proteins that are processed to produce the active p50 and p52 NF-kappa-B subunits, respectively. However, the p100 and p105 proteins serve regulatory functions and should not be considered exclusively as precursor forms. The most abundant activated form of NF-kappa-B is a heterodimer of the p50 or p52 subunit bound to the RELA subunit (MIM 164014). Other NF-kappa-B complexes, consisting of hetero- and homodimers of p50, p52, RELA, REL (MIM 164910), and RELB (MIM 604758), have also been detected. NF-kappa-B complexes are inhibited by I-kappa-B proteins, NFKBIA (MIM 164008) or NFKBIB (MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by the kinases IKBKA (CHUK; MIM 600664) or IKBKB (MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. The activated NF-kappa-B complex translocates into the nucleus and binds DNA at kappa-B-binding motifs, such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine). For reviews, see Chen et al. (1999) [PubMed 9895331] and Baldwin (1996) [PubMed 8717528]. |
NFKB1 Antibody |
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R20321-0.1ML | NSJ Bioreagents | 100ul | EUR 347.65 |
Description: This antibody reacts to human nuclear factor-kappa-B p105 subunit and p50 subunit. |
NFKB2 Antibody |
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R31047 | NSJ Bioreagents | 100 ug | EUR 356.15 |
Description: Nuclear Factor Kappa-B, Subunit 2, also known as NFKB or p52/p100 subunit, is a protein that in humans is encoded by the NFKB2 gene. Liptay et al.(1992) mapped the gene for what they called the p49/p100 subunit of NFKB to chromosome 10 by Southern blot analysis of panels of human/Chinese hamster cell hybrids. By fluorescence in situ hybridization(FISH), they confirmed the localization and mapped the gene with greater resolution to 10q24. NFKB2 appears to be the same as LYT10. Claudio et al.(2002) showed that bone marrow(BM) cells from Nfkb2-deficient mice, but not Nfkb1-deficient mice, failed to increase relative and total IgD-positive transitional-1(T1) stage B cells in response to Baff. In vivo, however, Nfkb2-deficient mice did generate mature B cells, but at reduced numbers. Mice of the aly/aly strain, which are naturally deficient in Nik, and mice of the A/WySNJ strain, which have a mutation in Baffr, also failed to produce T1 B cells in response to Baff. Baff stimulation enhanced expression of Bcl2 in T1 B cells, thereby promoting B-cell survival, and caused the processing of the p100 form of the protein to p52, which again required Baffr and Nik, but not Nemo(IKKG). Immunoblot analysis showed that BM cells contained primarily p100. |
NFKB1 Antibody |
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R31224 | NSJ Bioreagents | 100 ug | EUR 356.15 |
Description: Nuclear factor NF-kappa-B p105/p50, also called EBP-1 is a protein that in humans is encoded by the NFKB1 gene. By fluorescence in situ hybridization, the gene was assigned to human chromosome 4q24. NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. |
NFKB2 Antibody |
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R31570 | NSJ Bioreagents | 100 ug | EUR 356.15 |
Description: Nuclear factor kappa-B subunit 2 is a protein that in humans is encoded by the NFKB2 gene. It is mapped to 10q24.32. This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFKB). NFKB is activated by a wide variety of stimuli such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. |
Rabbit Anti-Human NFKB1 Polyclonal Antibody, Phospho-Ser932 |
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CPB-782RH | Creative Diagnostics | 100 ul | EUR 670.8 |
Rabbit Anti-Human NFKB1 Polyclonal Antibody, Phospho-Ser927 |
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CPB-827RH | Creative Diagnostics | 100 ul | EUR 670.8 |
NFKB1 antibody (HRP) |
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60R-2109 | Fitzgerald | 100 ug | EUR 392.4 |
Description: Rabbit polyclonal NFKB1 antibody (HRP) |
NFKB1 antibody (FITC) |
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60R-2110 | Fitzgerald | 100 ug | EUR 392.4 |
Description: Rabbit polyclonal NFKB1 antibody (FITC) |
NFKB1 Antibody (p105) |
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F52441-0.08ML | NSJ Bioreagents | 0.08 ml | EUR 140.25 |
Description: NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NFkB is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NFkB complexes are held in the cytoplasm in an inactive state complexed with members of the NFkB inhibitor (I-kappa-B) family. In a conventional activation pathway, IkB is phosphorylated by IkB kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NFkB complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NFkB p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NFkB proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105. |
NFKB1 Antibody (p105) |
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F47079-0.08ML | NSJ Bioreagents | 0.08 ml | EUR 140.25 |
Description: This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene. |
NFKB1 antibody (biotin) |
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60R-2111 | Fitzgerald | 100 ug | EUR 392.4 |
Description: Rabbit polyclonal NFKB1 antibody (biotin) |
NFKB1 Antibody NF-kB p105/p50 |
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R31223 | NSJ Bioreagents | 100 ug | EUR 356.15 |
Description: NF-kappa-B p105/p50, also called EBP-1 is a protein that in humans is encoded by the NFKB1 gene. By fluorescence in situ hybridization, the gene was assigned to human chromosome 4q24. NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. |
NFKB1p105 Antibody |
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35587-100ul | SAB | 100ul | EUR 302.4 |
NFKB2 (p100) Antibody |
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F47541-0.08ML | NSJ Bioreagents | 0.08 ml | EUR 140.25 |
Description: NF-kappa-B has been detected in numerous cell types that express cytokines, chemokines, growth factors, cell adhesion molecules, and some acute phase proteins in health and in various disease states. NF-kappa-B is activated by a wide variety of stimuli, such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. Inappropriate activation of NF-kappa-B has been linked to inflammatory events associated with autoimmune arthritis, asthma, septic shock, lung fibrosis, glomerulonephritis, atherosclerosis, and AIDS. In contrast, complete and persistent inhibition of NF-kappa-B has been linked directly to apoptosis, inappropriate immune cell development, and delayed cell growth. NFKB1 (MIM 164011) and NFKB2 encode p105 and p100 proteins that are processed to produce the active p50 and p52 NF-kappa-B subunits, respectively. However, the p100 and p105 proteins serve regulatory functions and should not be considered exclusively as precursor forms. The most abundant activated form of NF-kappa-B is a heterodimer of the p50 or p52 subunit bound to the RELA subunit (MIM 164014). Other NF-kappa-B complexes, consisting of hetero- and homodimers of p50, p52, RELA, REL (MIM 164910), and RELB (MIM 604758), have also been detected. NF-kappa-B complexes are inhibited by I-kappa-B proteins, NFKBIA (MIM 164008) or NFKBIB (MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by the kinases IKBKA (CHUK; MIM 600664) or IKBKB (MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. The activated NF-kappa-B complex translocates into the nucleus and binds DNA at kappa-B-binding motifs, such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine). For reviews, see Chen et al. (1999) [PubMed 9895331] and Baldwin (1996) [PubMed 8717528]. |
Rabbit Anti-Human NFKBIB polyclonal antibody |
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CABT-BL036 | Creative Diagnostics | 100 ul | EUR 702 |
NFKB2 (pS872) Antibody |
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abx333034-100ul | Abbexa | 100 ul | EUR 560.4 |
NFKB1 (pS337) Antibody |
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20-abx000177 | Abbexa |
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NFKB1 (pS927) Antibody |
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20-abx000315 | Abbexa |
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NFKB1 (pS893) Antibody |
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20-abx000462 | Abbexa |
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NFKB1 (pS907) Antibody |
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20-abx000463 | Abbexa |
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NFKB1 (pS932) Antibody |
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20-abx000464 | Abbexa |
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NFKB2 (pS866) Antibody |
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20-abx000465 | Abbexa |
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NFKB2 (pS870) Antibody |
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20-abx000466 | Abbexa |
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NFKB1 Antibody, HRP conjugated |
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1-CSB-PA10354B0Rb | Cusabio |
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Description: A polyclonal antibody against NFKB1. Recognizes NFKB1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
NFKB2 Antibody, HRP conjugated |
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1-CSB-PA10449B0Rb | Cusabio |
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Description: A polyclonal antibody against NFKB2. Recognizes NFKB2 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
Polyclonal NFKB1 Antibody (S932) |
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APR04474G | Leading Biology | 0.1ml | EUR 580.8 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human NFKB1 (S932). This antibody is tested and proven to work in the following applications: |
NFKB1 Antibody, FITC conjugated |
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1-CSB-PA10354C0Rb | Cusabio |
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Description: A polyclonal antibody against NFKB1. Recognizes NFKB1 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
NFKB2 Antibody, FITC conjugated |
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1-CSB-PA10449C0Rb | Cusabio |
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Description: A polyclonal antibody against NFKB2. Recognizes NFKB2 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
NFKB2 (Ab-870) Antibody |
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CSB-PA178291- | Cusabio | each | EUR 402 |
Description: A polyclonal antibody against NFKB2 (Ab-870). Recognizes NFKB2 (Ab-870) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:200 |
NFKB2 (Ab-870) Antibody |
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CSB-PA178291-100ul | Cusabio | 100ul | EUR 379.2 |
Description: A polyclonal antibody against NFKB2 (Ab-870). Recognizes NFKB2 (Ab-870) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:200 |
NFKB1 (Ab-337) Antibody |
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CSB-PA186775- | Cusabio | each | EUR 402 |
Description: A polyclonal antibody against NFKB1 (Ab-337). Recognizes NFKB1 (Ab-337) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:200 |
NFKB1 (Ab-337) Antibody |
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CSB-PA186775-100ul | Cusabio | 100ul | EUR 379.2 |
Description: A polyclonal antibody against NFKB1 (Ab-337). Recognizes NFKB1 (Ab-337) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:200 |
NFKB1 (Ab-932) Antibody |
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CSB-PA190132- | Cusabio | each | EUR 402 |
Description: A polyclonal antibody against NFKB1 (Ab-932). Recognizes NFKB1 (Ab-932) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:200 |
NFKB1 (Ab-932) Antibody |
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CSB-PA190132-100ul | Cusabio | 100ul | EUR 379.2 |
Description: A polyclonal antibody against NFKB1 (Ab-932). Recognizes NFKB1 (Ab-932) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:200 |
NFKB1 (Ab-893) Antibody |
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CSB-PA108426- | Cusabio | each | EUR 402 |
Description: A polyclonal antibody against NFKB1 (Ab-893). Recognizes NFKB1 (Ab-893) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:200 |
NFKB1 (Ab-893) Antibody |
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CSB-PA108426-100ul | Cusabio | 100ul | EUR 379.2 |
Description: A polyclonal antibody against NFKB1 (Ab-893). Recognizes NFKB1 (Ab-893) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:200 |
NFKB2 (Ab-872) Antibody |
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CSB-PA274947- | Cusabio | each | EUR 402 |
Description: A polyclonal antibody against NFKB2 (Ab-872). Recognizes NFKB2 (Ab-872) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200 |
NFKB2 (Ab-872) Antibody |
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CSB-PA274947-100ul | Cusabio | 100ul | EUR 379.2 |
Description: A polyclonal antibody against NFKB2 (Ab-872). Recognizes NFKB2 (Ab-872) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200 |
NFKB1 (Ab-907) Antibody |
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CSB-PA696291- | Cusabio | each | EUR 402 |
Description: A polyclonal antibody against NFKB1 (Ab-907). Recognizes NFKB1 (Ab-907) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:200 |
NFKB1 (Ab-907) Antibody |
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CSB-PA696291-100ul | Cusabio | 100ul | EUR 379.2 |
Description: A polyclonal antibody against NFKB1 (Ab-907). Recognizes NFKB1 (Ab-907) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:200 |
NFKB2 (Ab-866) Antibody |
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CSB-PA000249- | Cusabio | each | EUR 402 |
Description: A polyclonal antibody against NFKB2 (Ab-866). Recognizes NFKB2 (Ab-866) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200 |
NFKB2 (Ab-866) Antibody |
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CSB-PA000249-100ul | Cusabio | 100ul | EUR 379.2 |
Description: A polyclonal antibody against NFKB2 (Ab-866). Recognizes NFKB2 (Ab-866) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200 |
Antibody for Human NFKB1 |
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SPC-1303D | Stressmarq | 0.1ml | EUR 376.8 |
Description: A polyclonal antibody for NFKB1 from Human | Mouse. The antibody is produced in rabbit after immunization with Human A synthesized peptide derived from human NF-kappaB p105/p50. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:2000), IHC (1:200), ICC/IF (1:500). This NFKB1 antibody is unconjugated. |
Antibody for Human NFKB1 |
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SPC-1303D-A390 | Stressmarq | 0.1ml | EUR 433.2 |
Description: A polyclonal antibody for NFKB1 from Human | Mouse. The antibody is produced in rabbit after immunization with Human A synthesized peptide derived from human NF-kappaB p105/p50. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:2000), IHC (1:200), ICC/IF (1:500). This NFKB1 antibody is conjugated to ATTO 390. |
Antibody for Human NFKB1 |
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SPC-1303D-A488 | Stressmarq | 0.1ml | EUR 432 |
Description: A polyclonal antibody for NFKB1 from Human | Mouse. The antibody is produced in rabbit after immunization with Human A synthesized peptide derived from human NF-kappaB p105/p50. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:2000), IHC (1:200), ICC/IF (1:500). This NFKB1 antibody is conjugated to ATTO 488. |
Antibody for Human NFKB1 |
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SPC-1303D-A565 | Stressmarq | 0.1ml | EUR 432 |
Description: A polyclonal antibody for NFKB1 from Human | Mouse. The antibody is produced in rabbit after immunization with Human A synthesized peptide derived from human NF-kappaB p105/p50. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:2000), IHC (1:200), ICC/IF (1:500). This NFKB1 antibody is conjugated to ATTO 565. |
Antibody for Human NFKB1 |
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SPC-1303D-A594 | Stressmarq | 0.1ml | EUR 432 |
Description: A polyclonal antibody for NFKB1 from Human | Mouse. The antibody is produced in rabbit after immunization with Human A synthesized peptide derived from human NF-kappaB p105/p50. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:2000), IHC (1:200), ICC/IF (1:500). This NFKB1 antibody is conjugated to ATTO 594. |
Antibody for Human NFKB1 |
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SPC-1303D-A633 | Stressmarq | 0.1ml | EUR 432 |
Description: A polyclonal antibody for NFKB1 from Human | Mouse. The antibody is produced in rabbit after immunization with Human A synthesized peptide derived from human NF-kappaB p105/p50. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:2000), IHC (1:200), ICC/IF (1:500). This NFKB1 antibody is conjugated to ATTO 633. |
Antibody for Human NFKB1 |
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SPC-1303D-A655 | Stressmarq | 0.1ml | EUR 432 |
Description: A polyclonal antibody for NFKB1 from Human | Mouse. The antibody is produced in rabbit after immunization with Human A synthesized peptide derived from human NF-kappaB p105/p50. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:2000), IHC (1:200), ICC/IF (1:500). This NFKB1 antibody is conjugated to ATTO 655. |
Antibody for Human NFKB1 |
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SPC-1303D-A680 | Stressmarq | 0.1ml | EUR 432 |
Description: A polyclonal antibody for NFKB1 from Human | Mouse. The antibody is produced in rabbit after immunization with Human A synthesized peptide derived from human NF-kappaB p105/p50. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:2000), IHC (1:200), ICC/IF (1:500). This NFKB1 antibody is conjugated to ATTO 680. |
This will allow the belief of artificial circuits in S. oneidensis the place predictable, quantitative conduct is desired (in both single- or double-plasmid contexts). Klebsiella pneumoniae is a bacterial pathogen with rising charges of resistance to carbapenem antibiotics, however the inhabitants construction and genetic drivers of carbapenem-resistant Okay pneumoniae (CRKP) stay underexplored in creating nations. Carbapenem-resistant Okay pneumoniae had been not too long ago launched into Peru however have grown quickly in prevalence, enabling research of this pathogen because it expands into an unaffected setting.