Gene engineered mesenchymal stem cells: greater transgene expression and efficacy with minicircle vs. plasmid DNA vectors in a mouse model of acute lung injury

Gene engineered mesenchymal stem cells: greater transgene expression and efficacy with minicircle vs. plasmid DNA vectors in a mouse model of acute lung injury

 Acute lung damage (ALI) and in its extreme kind, acute respiratory misery syndrome (ARDS), ends in elevated pulmonary vascular irritation and permeability and is a serious reason for mortality in lots of critically sick sufferers. Though cell-based therapies have proven promise in experimental ALI, methods are wanted to reinforce the efficiency of mesenchymal stem cells (MSCs) to develop more practical remedies. Genetic modification of MSCs has been demonstrated to considerably enhance the therapeutic advantages of those cells; nonetheless, the optimum vector for gene switch is just not clear. Given the acute nature of ARDS, transient transfection is fascinating to keep away from off-target results of long-term transgene expression, in addition to the potential antagonistic penalties of genomic integration.

 Right here, we explored whether or not a minicircle DNA (MC) vector containing human angiopoietin 1 (MC-ANGPT1) can present a more practical platform for gene-enhanced MSC remedy of ALI/ARDS. At 24 h after transfection, nuclear-targeted electroporation utilizing an MC-ANGPT1 vector resulted in a 3.7-fold better enhance in human ANGPT1 protein in MSC conditioned media in comparison with the usage of a plasmid ANGPT1  vector. Within the lipopolysaccharide (LPS)-induced ALI mannequin, administration of pANGPT1 transfected MSCs considerably decreased bronchoalveolar lavage (BAL) neutrophil counts by 57%, whereas MC-ANGPT1 transfected MSCs decreased it by 71% (p < 0.001) by Holm-Sidak’s a number of comparability take a look at.

Furthermore, in comparison with pANGPT1, the MC-ANGPT1 transfected MSCs considerably decreased pulmonary irritation, as noticed in decreased ranges of proinflammatory cytokines, akin to tumor necrosis factor-alpha (TNF-α), interferon-gamma (IFN-γ), interleukin-6 (IL-6), monocyte chemoattractant protein-1 (MCP-1), and macrophage inflammatory protein-2 (MIP-2). pANGPT1-transfected MSCs considerably decreased BAL albumin ranges by 71%, whereas MC-ANGPT1-transfected MSCs decreased it by 85%. Total, utilizing a minicircle vector, we demonstrated an environment friendly and sustained expression of the ANGPT1 transgene in MSCs and enhanced the therapeutic impact on the ALI mannequin in comparison with plasmid.

Plasmid Vectors for in Vivo Choice-Free Use with the Probiotic E. coli Nissle 1917

Escherichia coli Nissle 1917 (EcN) is a probiotic bacterium, generally employed to deal with sure gastrointestinal issues. It’s quick rising as an essential goal for the event of therapeutic engineered micro organism, benefiting from the wealth of information of E. coli biology and ease of manipulation. Bacterial artificial biology initiatives generally make the most of engineered plasmid vectors, that are easy to engineer and might reliably obtain excessive ranges of protein expression. Nevertheless, plasmids usually require antibiotics for upkeep, and the administration of an antibiotic is commonly incompatible with in vivo experimentation or remedy.

EcN natively accommodates plasmids pMUT1 and pMUT2, which don’t have any recognized operate however are secure inside the micro organism. Right here, we describe the event of the pMUT plasmids into a strong platform for engineering EcN for in vivo experimentation, alongside a CRISPR-Cas9 system to take away the native plasmids. We systematically engineered each pMUT plasmids to include choice markers, fluorescent markers, temperature delicate expression, and curli secretion programs to export a customizable purposeful materials into the extracellular area.

We then reveal that the engineered plasmids had been maintained in micro organism because the engineered micro organism go by the mouse GI tract with out choice, and that the secretion system stays purposeful, exporting functionalized curli proteins into the intestine. Our plasmid system presents a platform for the fast improvement of therapeutic EcN micro organism.

Gene engineered mesenchymal stem cells: greater transgene expression and efficacy with minicircle vs. plasmid DNA vectors in a mouse model of acute lung injury

Cloning in Plasmid Vectors: Blunt-Finish Cloning

This protocol describes procedures for cloning blunt-ended DNA fragments into linearized plasmid vectors. To acquire the utmost variety of “appropriate” ligation merchandise when cloning blunt-ended goal fragments, the 2 elements of DNA within the ligation response have to be current at an acceptable ratio. These outcomes assist the potential advantages of MC-ANGPT1 gene enhancement of MSC remedy to deal with ARDS.

If the molar ratio of plasmid vector to focus on DNA is just too excessive, then the ligation response might generate an undesirable variety of round empty plasmids, each monomeric and polymeric; if too low, the ligation response might generate an extra of linear and round homopolymers and heteropolymers of various sizes, orientations, and compositions. Because of this, the orientation of the overseas DNA and the variety of inserts in every recombinant clone should at all times be validated by restriction endonuclease mapping or another means.

Plasmid copy quantity variation of a modular vector set in Shewanella oneidensis MR-1

To advance artificial biology approaches that make the most of S. oneidensis as host for biotechnology functions, now we have investigated the variation in plasmid copy variety of a modular vector set ensuing from distinct origins of replication beneath totally different circumstances. The replicons yielded a ≈9X-fold vary for plasmid copy quantity variation in S. oneidensis (whereas the identical origins yielded a ≈3X-fold vary in Escherichia coli). This offers a sizeable vary to regulate gene expression ranges in S. oneidensis for artificial biology functions. As well as, plasmid harboring the pBBR1 origin resulted in secure copy numbers in S. oneidensis beneath totally different circumstances (mid-logarithmic, stationary, multi-plasmid).

Transcription Factor P65 (NFKB3) Polyclonal Antibody

CAU27518-200ul 200ul
EUR 272.2

Transcription Factor P65 (NFKB3) Polyclonal Antibody

CAU27519-100ul 100ul
EUR 212.2

Transcription Factor P65 (NFKB3) Polyclonal Antibody

CAU27519-200ul 200ul
EUR 265.4

Polyclonal Antibody to Transcription Factor P65 (NFKB3)

PAA616Hu01 100ul
EUR 221

Polyclonal Antibody to Transcription Factor P65 (NFKB3)

PAA616Mu01 100ul
EUR 227

Polyclonal Antibody to Transcription Factor P65 (NFKB3)

PAA616Ra01 100ul
EUR 239

Anti-NFKB3 Antibody

39294 100ul
EUR 479

Anti-NFKB3 Antibody

Y058181 100 µl
EUR 145

anti- NFKB1 antibody

FNab10143 100µg
EUR 606.3
Description: Antibody raised against NFKB1

anti- NFKB1 antibody

FNab05702 100µg
EUR 606.3
Description: Antibody raised against NFKB1

anti- NFKB1 antibody

FNab05703 100µg
EUR 702
Description: Antibody raised against NFKB1

anti- NFKB2 antibody

FNab05705 100µg
EUR 606.3
Description: Antibody raised against NFKB2

anti- NFKB2 antibody

FNab05706 100µg
EUR 658.5
Description: Antibody raised against NFKB2

Anti- p52 (NFkB2) Antibody

GWB-AB58EB 0.1 ml Ask for price

Anti- p50 (NFKB1) Antibody

GWB-747B10 0.1 ml Ask for price

Recombinant Transcription Factor P65 (NFKB3)

RPU54244-100ug 100ug
EUR 589.6

Recombinant Transcription Factor P65 (NFKB3)

RPU54244-1mg 1mg
EUR 2610.4

Recombinant Transcription Factor P65 (NFKB3)

RPU54244-50ug 50ug
EUR 473

Recombinant Transcription Factor P65 (NFKB3)

RPU44376-100ug 100ug
EUR 393.8

Recombinant Transcription Factor P65 (NFKB3)

RPU44376-1mg 1mg
EUR 1747.2

Recombinant Transcription Factor P65 (NFKB3)

RPU44376-50ug 50ug
EUR 316.8

Recombinant Transcription Factor P65 (NFKB3)

RPU55569-100ug 100ug
EUR 918.5

Recombinant Transcription Factor P65 (NFKB3)

RPU55569-1mg 1mg
EUR 3606.2

Recombinant Transcription Factor P65 (NFKB3)

RPU55569-50ug 50ug
EUR 554.9

Recombinant Transcription Factor P65 (NFKB3)

RPA616Fi01 10ug
EUR 231

Recombinant Transcription Factor P65 (NFKB3)

RPA616Hu01 10ug
EUR 191

Recombinant Transcription Factor P65 (NFKB3)

RPA616Mu01 10ug
EUR 128

Anti-NFKB p50 (NFKB1) Antibody

GWB-CC6745 100 ul Ask for price

Human Transcription factor p65 protein (NFKB3)

1-CSB-RP039844h
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Description: Recombinant Human Transcription factor p65 protein(NFKB3),partial expressed in E.coli

Human Transcription factor p65 protein (NFKB3)

1-CSB-RP039874h
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Description: Recombinant Human Transcription factor p65 protein(NFKB3),partial expressed in E.coli

Anti-NFkB p105/p50/NFKB1 Antibody

PB9149 100ug/vial
EUR 400.8

Anti-NFkB p100/p52/NFKB2 Antibody

PB9150 100ug/vial
EUR 400.8

Anti-NFkB p100/p52/NFKB2 Antibody

PA1946 100ug/vial
EUR 400.8

Anti-NFkB p100/p52/NFKB2 Antibody

PA1946-1 100ug/vial
EUR 352.8

Anti-NFkB p105/p50/NFKB1 Antibody

PA2111 100ug/vial
EUR 352.8

Anti-NFkB p105/p50/NFKB1 Antibody

PA2111-1 100ug/vial
EUR 352.8

Rat Transcription Factor P65 (NFKB3) ELISA Kit

EKU08193-48T 48T
EUR 542.78

Rat Transcription Factor P65 (NFKB3) ELISA Kit

EKU08193-5x96T 5x96T
EUR 3683.15

Rat Transcription Factor P65 (NFKB3) ELISA Kit

EKU08193-96T 96T
EUR 775.4

Rat Transcription Factor P65 (NFKB3) ELISA Kit

EKN49304-48T 48T
EUR 337.82

Rat Transcription Factor P65 (NFKB3) ELISA Kit

EKN49304-5x96T 5x96T
EUR 2292.35

Rat Transcription Factor P65 (NFKB3) ELISA Kit

EKN49304-96T 96T
EUR 482.6

ELISA Kit for Transcription Factor P65 (NFKB3)

SEA616Hu 96Т
EUR 630

ELISA Kit for Transcription Factor P65 (NFKB3)

SEA616Ra 96Т
EUR 684

Human Transcription Factor P65 (NFKB3) ELISA Kit

EKU11278-48T 48T
EUR 600.25

Human Transcription Factor P65 (NFKB3) ELISA Kit

EKU11278-5x96T 5x96T
EUR 4073.13

Human Transcription Factor P65 (NFKB3) ELISA Kit

EKU11278-96T 96T
EUR 857.5

Human Transcription Factor P65 (NFKB3) ELISA Kit

EKU08192-48T 48T
EUR 500.5

Human Transcription Factor P65 (NFKB3) ELISA Kit

EKU08192-5x96T 5x96T
EUR 3396.25

Human Transcription Factor P65 (NFKB3) ELISA Kit

EKU08192-96T 96T
EUR 715

Human Transcription Factor P65 (NFKB3) ELISA Kit

EKN49303-48T 48T
EUR 311.15

Human Transcription Factor P65 (NFKB3) ELISA Kit

EKN49303-5x96T 5x96T
EUR 2111.38

Human Transcription Factor P65 (NFKB3) ELISA Kit

EKN49303-96T 96T
EUR 444.5

Human Transcription Factor P65 (NFKB3) ELISA Kit

EKN52389-48T 48T
EUR 373.38

Human Transcription Factor P65 (NFKB3) ELISA Kit

EKN52389-5x96T 5x96T
EUR 2533.65

Human Transcription Factor P65 (NFKB3) ELISA Kit

EKN52389-96T 96T
EUR 533.4

Anti- cRel (NFkB) Antibody

GWB-393707 0.1 ml Ask for price

anti- NFKB1,p105,p50-Specific antibody

FNab05704 100µg
EUR 702
Description: Antibody raised against NFKB1,p105,p50-Specific

anti- NFKB2,p52,p100-Specific antibody

FNab05707 100µg
EUR 658.5
Description: Antibody raised against NFKB2,p52,p100-Specific

anti- NFKBIE antibody

FNab05708 100µg
EUR 658.5
Description: Antibody raised against NFKBIE

anti- NFKBIZ antibody

FNab05709 100µg
EUR 606.3
Description: Antibody raised against NFKBIZ

Pig Transcription factor p65 / NFKB3 (RELA) ELISA Kit

abx361108-96tests 96 tests
EUR 990

Rat Transcription factor p65 / NFKB3 (RELA) ELISA Kit

abx255851-96tests 96 tests
EUR 801.6

Rat Transcription factor p65 / NFKB3 (RELA) ELISA Kit

20-abx156040
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Rat Transcription factor p65 / NFKB3 (RELA) ELISA Kit

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Rat Transcription factor p65 / NFKB3 (RELA) ELISA Kit

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Rat Transcription factor p65 / NFKB3 (RELA) ELISA Kit

abx156040-100g 100 µg
EUR 6075

Rat Transcription factor p65 / NFKB3 (RELA) ELISA Kit

abx156040-10g 10 µg
EUR 675

Rat Transcription factor p65 / NFKB3 (RELA) ELISA Kit

abx156040-50g 50 µg
EUR 3212.5

Rabbit Anti-Human NFKB1 (aa 930-934) Polyclonal Antibody

CPB-1129RH 100 ul
EUR 552

Rabbit Anti-Human NFKB1 (aa 160-170) Polyclonal Antibody

CPB-1169RH 100 ul
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Rabbit Anti-Human NFKB2 (aa 160-171) Polyclonal Antibody

CPB-1176RH 100 ul
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Human Transcription factor p65 / NFKB3 (RELA) ELISA Kit

abx570125-96tests 96 tests
EUR 801.6

Mouse Transcription factor p65 / NFKB3 (RELA) ELISA Kit

abx570127-96tests 96 tests
EUR 801.6

Sheep Transcription factor p65 / NFKB3 (RELA) ELISA Kit

abx364265-96tests 96 tests
EUR 1111.2

Human Transcription factor p65 / NFKB3 (RELA) ELISA Kit

abx252815-96tests 96 tests
EUR 801.6

Mouse Transcription factor p65 / NFKB3 (RELA) ELISA Kit

abx254289-96tests 96 tests
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Human Transcription factor p65 / NFKB3 (RELA) ELISA Kit

20-abx152945
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  • 10 × 96 tests
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Anti-Phospho-N kappa-p105 (S893) NFKB1 Antibody

A00283S893 100ul
EUR 380
Description: Boster Bio Anti-Phospho-N kappa-p105 (S893) NFKB1 Antibody catalog # A00283S893. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Anti-Phospho-N kappa-p105 (S907) NFKB1 Antibody

A00283S907 100ul
EUR 380
Description: Boster Bio Anti-Phospho-N kappa-p105 (S907) NFKB1 Antibody catalog # A00283S907. Tested in ELISA, IP, IHC, WB applications. This antibody reacts with Human.

Anti-Phospho-N kappa-p105 (S932) NFKB1 Antibody

A00283S932 100ul
EUR 380
Description: Boster Bio Anti-Phospho-N kappa-p105 (S932) NFKB1 Antibody catalog # A00283S932. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Rabbit Transcription factor p65 / NFKB3 (RELA) ELISA Kit

abx362582-96tests 96 tests
EUR 990

Monkey Transcription factor p65 / NFKB3 (RELA) ELISA Kit

abx359107-96tests 96 tests
EUR 990

Chicken Transcription factor p65 / NFKB3 (RELA) ELISA Kit

abx357192-96tests 96 tests
EUR 990

Chicken Transcription factor p65 / NFKB3 (RELA) ELISA Kit

abx357192-1096tests 10 × 96 tests Ask for price

Chicken Transcription factor p65 / NFKB3 (RELA) ELISA Kit

abx357192-596tests 5 × 96 tests
EUR 662.5

Rabbit anti IkBa (NFKB1A) Polyclonal Antibody

TA354676 100 µg Ask for price

Transcription Factor P65 (NFKB3) Magnetic Luminex Assay Kit

LKU602130-96T 96T
EUR 869.4

Transcription Factor P65 (NFKB3) Magnetic Luminex Assay Kit

LKU602133-96T 96T
EUR 944.2

Guinea pig Transcription factor p65 / NFKB3 (RELA) ELISA Kit

abx357262-96tests 96 tests
EUR 990

Rabbit Anti-Human NFKB2 Polyclonal Antibody, aa.864-868

CPB-930RH 100 ul
EUR 552

Rabbit Anti-Human NFKB2 Polyclonal Antibody, aa.868-872

CPB-931RH 100 ul
EUR 552

Rabbit Anti-Human NFKB1 Polyclonal Antibody, aa.335-339

CPB-932RH 100 ul
EUR 552

Rabbit Anti-Human NFKB1 Polyclonal Antibody, aa.891-895

CPB-933RH 100 ul
EUR 552

Rabbit Anti-Human NFKB1 Polyclonal Antibody, aa.905-909

CPB-934RH 100 ul
EUR 552

Mini Samples ELISA Kit for Transcription Factor P65 (NFKB3)

MEA616Hu 96Т
EUR 756

Rabbit anti IkBa (pS32/36) (NFKB1A) Polyclonal Antibody

TA354674 100 µg Ask for price

Anti- NFkB p65 (Rel A) Antibody

GWB-34C0DE 0.1 ml Ask for price

NFKB1 antibody

10R-11003 100 ug
EUR 344
Description: Mouse monoclonal NFKB1 antibody

NFKB1 antibody

10R-11155 100 ug
EUR 344
Description: Mouse Monoclonal NFKB1 antibody

NFKB2 antibody

10R-1605 100 ug
EUR 614.4
Description: Mouse monoclonal NFKB2 antibody

NFKB1 antibody

20-PR03 100 ul Ask for price
Description: Rabbit polyclonal NFKB1 antibody

NFKB1 Antibody

35588 100ul
EUR 319

NFKB1 Antibody

35588-100ul 100ul
EUR 302.4

NFKB2 antibody

38583 100ul
EUR 439

NFKB2 antibody

38583-100ul 100ul
EUR 302.4

NFKB1 antibody

39087 100ul
EUR 439

NFKB1 antibody

39087-100ul 100ul
EUR 302.4

NFKB1 Antibody

1-CSB-PA015759GA01HU
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Description: A polyclonal antibody against NFKB1. Recognizes NFKB1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB

NFKB2 Antibody

1-CSB-PA015760GA01HU
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  • 150ul
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Description: A polyclonal antibody against NFKB2. Recognizes NFKB2 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB

NFKB1 Antibody

E035588 100μg/100μl
EUR 255
Description: Available in various conjugation types.

NFKB2 Antibody

E037182 100μg/100μl
EUR 255
Description: Available in various conjugation types.

NFKB2 Antibody

E19-7190 100μg/100μl
EUR 225
Description: Available in various conjugation types.

NFKB1 Antibody

E10-30224 100μg/100μl
EUR 225
Description: Available in various conjugation types.

NFKB1 Antibody

E10-30234 100μg/100μl
EUR 225
Description: Available in various conjugation types.

NFKB1 Antibody

F47079-0.4ML 0.4 ml
EUR 322.15
Description: This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene.

NFKB2 Antibody

F47541-0.4ML 0.4 ml
EUR 322.15
Description: NF-kappa-B has been detected in numerous cell types that express cytokines, chemokines, growth factors, cell adhesion molecules, and some acute phase proteins in health and in various disease states. NF-kappa-B is activated by a wide variety of stimuli, such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. Inappropriate activation of NF-kappa-B has been linked to inflammatory events associated with autoimmune arthritis, asthma, septic shock, lung fibrosis, glomerulonephritis, atherosclerosis, and AIDS. In contrast, complete and persistent inhibition of NF-kappa-B has been linked directly to apoptosis, inappropriate immune cell development, and delayed cell growth. NFKB1 (MIM 164011) and NFKB2 encode p105 and p100 proteins that are processed to produce the active p50 and p52 NF-kappa-B subunits, respectively. However, the p100 and p105 proteins serve regulatory functions and should not be considered exclusively as precursor forms. The most abundant activated form of NF-kappa-B is a heterodimer of the p50 or p52 subunit bound to the RELA subunit (MIM 164014). Other NF-kappa-B complexes, consisting of hetero- and homodimers of p50, p52, RELA, REL (MIM 164910), and RELB (MIM 604758), have also been detected. NF-kappa-B complexes are inhibited by I-kappa-B proteins, NFKBIA (MIM 164008) or NFKBIB (MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by the kinases IKBKA (CHUK; MIM 600664) or IKBKB (MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. The activated NF-kappa-B complex translocates into the nucleus and binds DNA at kappa-B-binding motifs, such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine). For reviews, see Chen et al. (1999) [PubMed 9895331] and Baldwin (1996) [PubMed 8717528].

NFKB1 Antibody

F52441-0.4ML 0.4 ml
EUR 330.65
Description: NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NFkB is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NFkB complexes are held in the cytoplasm in an inactive state complexed with members of the NFkB inhibitor (I-kappa-B) family. In a conventional activation pathway, IkB is phosphorylated by IkB kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NFkB complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NFkB p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NFkB proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.

NFKB1 Antibody

E96667 100ul
EUR 255
Description: Available in various conjugation types.

NFKB1 Antibody

E911160 100ul
EUR 255
Description: Available in various conjugation types.

NFKB2 Antibody

E93108 100ul
EUR 255
Description: Available in various conjugation types.

NFKB1 Antibody

F54028-0.1ML 0.1 ml
EUR 322.15
Description: NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. [UniProt]

This will allow the belief of artificial circuits in S. oneidensis the place predictable, quantitative conduct is desired (in both single- or double-plasmid contexts). Klebsiella pneumoniae is a bacterial pathogen with rising charges of resistance to carbapenem antibiotics, however the inhabitants construction and genetic drivers of carbapenem-resistant Okay pneumoniae (CRKP) stay underexplored in creating nations. Carbapenem-resistant Okay pneumoniae had been not too long ago launched into Peru however have grown quickly in prevalence, enabling research of this pathogen because it expands into an unaffected setting.

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