Successful delivery of large-size CRISPR/Cas9 vectors in hard-to-transfect human cells using small plasmids
blocking peptide / EIA / electrophoresis / extract / Percp / plex / profile / Pure / Purification / Rabbit / Rhesus

Successful delivery of large-size CRISPR/Cas9 vectors in hard-to-transfect human cells using small plasmids

- August 24, 2021 | August 26, 2021 - Ajan

With the rise of recent highly effective genome engineering applied sciences, equivalent to CRISPR/Cas9, cell fashions might be engineered successfully to speed up fundamental and illness analysis. Essentially the most essential step on this process is the environment friendly supply of international nucleic acids into cells by mobile transfection. For the reason that vectors encoding the elements crucial for CRISPR/Cas genome engineering are at all times massive (9-19 kb), they end in low transfection effectivity and cell viability, and thus subsequent choice or purification of optimistic cells is required.

To beat these obstacles, we right here present a non-toxic and non-viral supply methodology that will increase transfection effectivity (as much as 40-fold) and cell viability (as much as 6-fold) in plenty of hard-to-transfect human most cancers cell strains and first blood cells. At its core, the approach is predicated on including exogenous small plasmids of an outlined measurement to the transfection combination. The “entire genome” TMV-based expression system, Geneware®, was used within the cGMP manufacturing of the plant-made pharmaceutical Q-Griffithsin and demonstrates steady expression for as much as a two-year interval.

Virion and plasmid banks which contained viral cDNA and a Q-Griffithsin sequence had been capable of produce >200 g of Q-Griffithsin. Knowledge assessing the standard and stability of the product banks had been measured by means of useful assessments of visible symptomology and product expression.

The cell-penetrating YopM protein-functionalized quantum dot-plasmid DNA conjugate as a novel gene supply vector

Non-viral gene supply techniques have nice potential for secure and environment friendly gene remedy, whereas inefficient mobile and nuclear uptake stay as the foremost hurdles. Novel approaches are wanted to reinforce the transfection effectivity of non-viral vectors. In accordance with this want, the target of this examine was to assemble a non-viral vector that might obtain gene supply with out utilizing further lipid-based transfection agent. We aimed to impart self-delivery property to a non-viral vector through the use of the cell and nucleus penetrating properties of YopM proteins from the three Yersinia spp. (Y. pestis, Y. enterocolotica and Y. pseudotuberculosis).

Plasmid DNA (pDNA) encoding inexperienced fluorescent protein (GFP) was labeled with quantum dots (QDs) through peptide-nucleic acid (PNA) recognition web site. Recombinant YopM protein was then hooked up to the conjugate through a second PNA recognition web site. The YopM ̶ QDs ̶ pDNA conjugate was transfected into HeLa cells with out utilizing further transfection reagent. All three conjugates produced GFP fluorescence, indicating that the plasmid was efficiently delivered to the nucleus.

As management, bare pDNA was transfected into the cells through the use of a industrial transfection reagent. The Y. pseudotuberculosis YopM-functionalized conjugate achieved the best GFP expression, in comparison with different two YopM proteins and the transfection reagent. To one of the best of our data, YopM protein was used for the primary time in a non-viral gene supply vector.

[Maintenance of Plasmid Expression in vivo Depends Primarily on the CpG Contents of the Vector and Transgene]

Plasmid-mediated gene remedy, being a secure and comparatively cheap therapeutic technique, is affected by a quick silencing of transgene expression. The silencing severely reduces the long-term effectivity of plasmid vectors. Now we have earlier constructed a low-CpG pMBR2 plasmid vector supporting extended expression of transgenes in mesenchymal stem cells in vitro. Lengthy-term expression from the pMBR2 vector was studied for the wild-type mouse secreted alkaline phosphatase gene (mSEAPTwt) and its model devoid of CpGs (mSEAP0) after vector electroporation into mouse hindlimb muscle mass and hydrodynamic supply to the liver.

Successful delivery of large-size CRISPR/Cas9 vectors in hard-to-transfect human cells using small plasmids

The mSEAP ranges within the blood had been measured over one yr. With the pMBR2-mSEAP0 assemble, the mSEAP ranges in leg muscle mass elevated greater than 2.5-fold within the first two months and remained increased than the preliminary stage till the top of the experiment. Far decrease expression ranges had been noticed with the management pCDNA3.1-mSEAP0 assemble. Expression from pMBR2-mSEAPwt decreased to about 40% after 6 months and remained at comparable ranges thereafter. Within the mouse liver, expression from pMBR2-mSEAP0 was roughly halved throughout the first 18 weeks after which lower slowly to the ultimate 17% stage.

Expression from pMBR2-mSEAPwt initially dropped to 18% and remained at roughly 10% thereafter. In distinction, expression from pCDNA3.1-mSEAP0 sharply dropped to five% after 2 weeks and remained at almost zero ranges all through the remainder of the experiment. Thus, each vector and transgene ought to have considerably diminished CpG contents to make sure extended plasmid-mediated expression within the liver, whereas minimizing the vector CpG content material is adequate for expression in skeletal muscle mass. The outcomes prompt moreover that the localization of S/MAR components throughout the transcription unit, in distinction to their outdoors location, ends in important discount of the extent of secreted, however not cytoplasmic, proteins.

Synthesis of aqueous AgInS/ZnS@PEI as a self-indicating nonviral vector for plasmid DNA self-tracking supply.

Varied nanoparticles have been employed for gene supply. Revealing the endocytosis or phagocytosis behaviors of the gene supply system is essential for designing refined gene therapies. Herein, a type of water-soluble PEI capped AgInS/ZnS quantum dot was synthesized as a self-indicating gene vector within the presence of branched polyethylenimine (PEI) and mercaptopropionic acid (MPA). The as-prepared ZAIS@PEI QDs might effectively condense plasmid DNA into nanocomplexes with robust fluorescence, which may reveal their positions throughout gene transfection.

Alexa Fluor® 488 Reference Standard
BLI886C-14 2x7ml
EUR 1007

Alexa Fluor® 488 anti-rat CD3
E16FRG003-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-rat CD4
E16FRG004-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Quantum™ Alexa Fluor® 488 MESF
BLI488A-1 1ml
EUR 564

Quantum™ Alexa Fluor® 488 MESF
BLI488B-5 5ml
EUR 1091

Quantum™ Alexa Fluor® 488 MESF
BLI488C-14 2x7ml
EUR 1769

Mouse Human CD3 (Alexa Fluor® 488)
E2790292 100ul
EUR 225
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-rat CD25
E16FRG025-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-rat CD45
E16FRG045-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse IgD
E16SDG501-025U 25 μg
EUR 411.67
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse IgD
E16SDG501-100U 100 μg
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse IgE
E16SEG501-025U 25 μg
EUR 325
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse IgE
E16SEG501-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse IgM
E16SMG501-025U 25 μg
EUR 325
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse IgM
E16SMG501-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD3
E16FMG003-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD3
E16FMG003-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD4
E16FMG004-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD5
E16FMG005-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD3
E16FHG003-025 25 tests
EUR 288.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD3
E16FHG003-050 50 tests
EUR 411.67
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD3
E16FHG003-100 100 Tests
EUR 682.67
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD4
E16FHG004-050 25 tests
EUR 343
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD4
E16FHG004-100 100 tests
EUR 457.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD8
E16FHG008-025 25 tests
EUR 288.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD8
E16FHG008-050 50 tests
EUR 411.67
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD8
E16FHG008-100 100 tests
EUR 682.67
Description: Available in various conjugation types.

Goat anti Mouse IgG1 (Alexa Fluor 488)
43R-1649 500 ug
EUR 684
Description: Goat anti Mouse IgG1 secondary antibody (Alexa Fluor 488)

Alexa Fluor® 488 anti-mouse CD8a
E16FMG008a-025U 25 μg
EUR 130
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD8a
E16FMG008a-100U 100 μg
EUR 281.67
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD19
E16FMG019-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD23
E16FMG023-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD24
E16FMG024-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD24
E16FMG024-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD25
E16FMG025-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD25
E16FMG025-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD28
E16FMG028-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD31
E16FMG031-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD36
E16FMG036-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD36
E16FMG036-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD40
E16FMG040-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD41
E16FMG041-025U 25 μg
EUR 411.67
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD41
E16FMG041-100U 100 μg
EUR 1061.67
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD45
E16FMG045-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD45.1
E16FMG045.1-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD45.2
E16FMG045.2-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD48
E16FMG048-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD54
E16FMG054-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD68
E16FMG068-025U 25 μg
EUR 541.67
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD68
E16FMG068-100U 100 μg
EUR 1278.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD80
E16FMG080-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD80
E16FMG080-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD83
E16FMG083-025U 25 μg
EUR 476.67
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD83
E16FMG083-100U 100 μg
EUR 1191.67
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD86
E16FMG086-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD86
E16FMG086-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD90.2
E16FMG090.2-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-rat CD45RA
E16FRG045RA-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD1a
E16FHG001a-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD1c
E16FHG001c-025 25 tests
EUR 498.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD1c
E16FHG001c-100 100 tests
EUR 1061.67
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD8a
E16FHG008a-025 25 tests
EUR 411.67
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD8a
E16FHG008a-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD14
E16FHG0142-025 25 tests
EUR 325
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD14
E16FHG0142-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD15
E16FHG0151-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD16
E16FHG016-025 25 tests
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD16
E16FHG016-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD19
E16FHG019-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD20
E16FHG020-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD24
E16FHG024-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD25
E16FHG025-025 25 tests
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD25
E16FHG025-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD28
E16FHG028-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD29
E16FHG029-025 25 tests
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD29
E16FHG029-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD31
E16FHG031-025 25 tests
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD31
E16FHG031-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD34
E16FHG034-025 25 tests
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD34
E16FHG034-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD38
E16FHG038-025 25 tests
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD38
E16FHG038-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD40
E16FHG040-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD45
E16FHG045-025 25 tests
EUR 411.67
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD45
E16FHG045-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD54
E16FHG054-025 25 tests
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD54
E16FHG054-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD64
E16FHG064-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD68
E16FHG068-025 25 tests
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD68
E16FHG068-100 100 tests
EUR 1061.67
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD69
E16FHG069-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD80
E16FHG080-025 25 tests
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD80
E16FHG080-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD83
E16FHG083-100 100 tests
EUR 1083.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD86
E16FHG086-025 25 tests
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD86
E16FHG086-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD11a
E16FMG011a-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD11c
E16FMG011c-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD11c
E16FMG011c-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD49b
E16FMG049b-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD49d
E16FMG049d-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD62L
E16FMG062L-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD102
E16FMG102-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD103
E16FMG103-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD105
E16FMG105-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD105
E16FMG105-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD106
E16FMG106-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD146
E16FMG146-025U 25 μg
EUR 520
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD146
E16FMG146-100U 100 μg
EUR 1083.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD11a
E16FHG011a-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD11b
E16FHG011b-025 25 tests
EUR 346.67
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD11b
E16FHG011b-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD11b
E16FHG011b-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD11b
E16FHG011b-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD11c
E16FHG011c-025 25 tests
EUR 411.67
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD11c
E16FHG011c-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD62L
E16FHG062L-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD105
E16FHG105-025 25 tests
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD105
E16FHG105-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD147
E16FHG147-025 25 tests
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD154
E16FHG154-025 25 tests
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD161
E16FHG161-025 25 tests
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD161
E16FHG161-100 100 tests
EUR 1018.33
Description: Available in various conjugation types.

Mouse Anti-Human IgA - Alexa Fluor 488
DL87215A-100ul 100 ul
EUR 190
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgA - Alexa Fluor 488
DL87215A-500ul 500 ul
EUR 280
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgM - Alexa Fluor 488
DL87218A-100ul 100 ul
EUR 155
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgM - Alexa Fluor 488
DL87218A-500ul 500 ul
EUR 350
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgA - Alexa Fluor 488
E38A3234 100μL
EUR 125

Mouse Anti-Human IgM - Alexa Fluor 488
E38A3238 100μL
EUR 125

Mouse Anti-Human IgA - Alexa Fluor 488
YLF0508-100ul 100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgA - Alexa Fluor 488
YLF0508-1ml 1 ml
EUR 300
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgA - Alexa Fluor 488
YLF0508-500ul 500 ul
EUR 210
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgM - Alexa Fluor 488
YLF0257-100ul 100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgM - Alexa Fluor 488
YLF0257-1ml 1 ml
EUR 480
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgM - Alexa Fluor 488
YLF0257-500ul 500 ul
EUR 290
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Alexa Fluor® 488 anti-human CD45RA
E16FHG0453-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD45RO
E16FHG0456-100 100 tests
EUR 975
Description: Available in various conjugation types.

Rabbit Anti-Human IgM - Alexa Fluor 488
DL87207A-100ul 100 ul
EUR 190
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgM - Alexa Fluor 488
DL87207A-500ul 500 ul
EUR 280
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgM - Alexa Fluor 488
YLF0505-100ul 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgM - Alexa Fluor 488
YLF0505-1ml 1 ml
EUR 300
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgM - Alexa Fluor 488
YLF0505-500ul 500 ul
EUR 210
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Anti-Hu CD16 Alexa Fluor® 488
A4-646-T100 100 tests
EUR 322.8

Alexa Fluor® 488 anti-rat CD11b/c
E16FRG011b/c-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Goat Anti-Human IgG Fc - Alexa Fluor 488
DL87186A-100ul 100 ul
EUR 155
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fc - Alexa Fluor 488
DL87186A-500ul 500 ul
EUR 350
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fc - Alexa Fluor 488
E38A3204 100μL
EUR 125

Goat Anti-Human IgG Fc - Alexa Fluor 488
YLF0235-100ul 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fc - Alexa Fluor 488
YLF0235-1ml 1 ml
EUR 480
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fc - Alexa Fluor 488
YLF0235-500ul 500 ul
EUR 290
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Alexa Fluor® 488 rat anti-mouse CD19
E16FMG019-100U 100 μg
EUR 823.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse/rat CD29
E16FMG029-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse/rat CD29
E16FMG029-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 488 rat anti-mouse CD38
E16FMG038-100U 100 μg
EUR 823.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse/rat CD61
E16FMG061-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse/rat CD29
E16FRG029-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse/rat CD29
E16FRG029-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse/rat CD61
E16FRG061-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD95 (Fas)
E16FHG095-025 25 tests
EUR 498.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD95 (Fas)
E16FHG095-100 100 tests
EUR 1083.33
Description: Available in various conjugation types.

Goat Anti-Human IgG Fab - Alexa Fluor 488
DL87185A-100ul 100 ul
EUR 190
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fab - Alexa Fluor 488
DL87185A-500ul 500 ul
EUR 280
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fc - Alexa Fluor 488
DL87217A-100ul 100 ul
EUR 190
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fc - Alexa Fluor 488
DL87217A-500ul 500 ul
EUR 280
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fab - Alexa Fluor 488
E38A3203 100μL
EUR 125

Mouse Anti-Human IgG Fc - Alexa Fluor 488
E38A3236 100μL
EUR 125

Goat Anti-Human IgG Fab - Alexa Fluor 488
YLF0502-100ul 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fab - Alexa Fluor 488
YLF0502-1ml 1 ml
EUR 300
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fab - Alexa Fluor 488
YLF0502-500ul 500 ul
EUR 210
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fc - Alexa Fluor 488
YLF0510-100ul 100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fc - Alexa Fluor 488
YLF0510-1ml 1 ml
EUR 300
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fc - Alexa Fluor 488
YLF0510-500ul 500 ul
EUR 210
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Alexa Fluor® 488 rat anti-mouse CD49e
E16FMG049e-100U 100 μg
EUR 823.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD206 (MMR)
E16FMG206-025U 25 μg
EUR 476.67
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD206 (MMR)
E16FMG206-100U 100 μg
EUR 1083.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD56 (NCAM)
E16FHG056-025 25 tests
EUR 411.67
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD56 (NCAM)
E16FHG056-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD206 (MMR)
E16FHG206-025 25 tests
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human CD206 (MMR)
E16FHG206-100 100 tests
EUR 975
Description: Available in various conjugation types.

anti-GFP VHH antibody (Alexa Fluor®488)
JOT0001-4 each Ask for price

Rabbit Anti-Human IgG Fc - Alexa Fluor 488
DL87204A-100ul 100 ul
EUR 155
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgG Fc - Alexa Fluor 488
DL87204A-500ul 500 ul
EUR 350
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fab - Alexa Fluor 488
DL87216A-100ul 100 ul
EUR 190
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fab - Alexa Fluor 488
DL87216A-500ul 500 ul
EUR 280
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

anti-GFP VHH antibody (Alexa Fluor®488)
JOT0001-4-15 15
EUR 221.92
Description: Conjugated VHH single domain antibody

anti-GFP VHH antibody (Alexa Fluor®488)
JOT0001-4-5 5
EUR 121.77
Description: Conjugated VHH single domain antibody

anti-GFP VHH antibody (Alexa Fluor®488)
JOT0001-4-50 50
EUR 340.96
Description: Conjugated VHH single domain antibody

Rabbit Anti-Human IgG Fc - Alexa Fluor 488
E38A3222 100μL
EUR 125

Mouse Anti-Human IgG Fab - Alexa Fluor 488
E38A3235 100μL
EUR 125

Mouse Anti-Human IgG Fab - Alexa Fluor 488
YLF0509-100ul 100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fab - Alexa Fluor 488
YLF0509-1ml 1 ml
EUR 300
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fab - Alexa Fluor 488
YLF0509-500ul 500 ul
EUR 210
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgG Fc - Alexa Fluor 488
YLF0250-100ul 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgG Fc - Alexa Fluor 488
YLF0250-1ml 1 ml
EUR 480
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgG Fc - Alexa Fluor 488
YLF0250-500ul 500 ul
EUR 290
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Alexa Fluor® 488 anti-human/mouse CD44
E16FMG044-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-human/mouse CD44
E16FMG044-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD150 (SLAM)
E16FMG150-025U 25 μg
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 488 anti-mouse CD150 (SLAM)
E16FMG150-100U 100 μg
EUR 975
Description: Available in various conjugation types.

Our outcomes confirmed that ZAIS@PEI might mediate plasmid DNA supply into HeLa cells with the excessive transfection effectivity of 40% and low cytotoxicity, in the meantime permitting the real-time monitoring of gene transfection. The obtained ZAIS@PEI QDs had been verified as versatile self-tracking gene vectors, which built-in gene supply with bioimaging capabilities with out exterior labeling.

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