With the rise of recent highly effective genome engineering applied sciences, equivalent to CRISPR/Cas9, cell fashions might be engineered successfully to speed up fundamental and illness analysis. Essentially the most essential step on this process is the environment friendly supply of international nucleic acids into cells by mobile transfection. For the reason that vectors encoding the elements crucial for CRISPR/Cas genome engineering are at all times massive (9-19 kb), they end in low transfection effectivity and cell viability, and thus subsequent choice or purification of optimistic cells is required.
To beat these obstacles, we right here present a non-toxic and non-viral supply methodology that will increase transfection effectivity (as much as 40-fold) and cell viability (as much as 6-fold) in plenty of hard-to-transfect human most cancers cell strains and first blood cells. At its core, the approach is predicated on including exogenous small plasmids of an outlined measurement to the transfection combination. The “entire genome” TMV-based expression system, Geneware®, was used within the cGMP manufacturing of the plant-made pharmaceutical Q-Griffithsin and demonstrates steady expression for as much as a two-year interval.
Virion and plasmid banks which contained viral cDNA and a Q-Griffithsin sequence had been capable of produce >200 g of Q-Griffithsin. Knowledge assessing the standard and stability of the product banks had been measured by means of useful assessments of visible symptomology and product expression.
The cell-penetrating YopM protein-functionalized quantum dot-plasmid DNA conjugate as a novel gene supply vector
Non-viral gene supply techniques have nice potential for secure and environment friendly gene remedy, whereas inefficient mobile and nuclear uptake stay as the foremost hurdles. Novel approaches are wanted to reinforce the transfection effectivity of non-viral vectors. In accordance with this want, the target of this examine was to assemble a non-viral vector that might obtain gene supply with out utilizing further lipid-based transfection agent. We aimed to impart self-delivery property to a non-viral vector through the use of the cell and nucleus penetrating properties of YopM proteins from the three Yersinia spp. (Y. pestis, Y. enterocolotica and Y. pseudotuberculosis).
Plasmid DNA (pDNA) encoding inexperienced fluorescent protein (GFP) was labeled with quantum dots (QDs) through peptide-nucleic acid (PNA) recognition web site. Recombinant YopM protein was then hooked up to the conjugate through a second PNA recognition web site. The YopM ̶ QDs ̶ pDNA conjugate was transfected into HeLa cells with out utilizing further transfection reagent. All three conjugates produced GFP fluorescence, indicating that the plasmid was efficiently delivered to the nucleus.
As management, bare pDNA was transfected into the cells through the use of a industrial transfection reagent. The Y. pseudotuberculosis YopM-functionalized conjugate achieved the best GFP expression, in comparison with different two YopM proteins and the transfection reagent. To one of the best of our data, YopM protein was used for the primary time in a non-viral gene supply vector.
[Maintenance of Plasmid Expression in vivo Depends Primarily on the CpG Contents of the Vector and Transgene]
Plasmid-mediated gene remedy, being a secure and comparatively cheap therapeutic technique, is affected by a quick silencing of transgene expression. The silencing severely reduces the long-term effectivity of plasmid vectors. Now we have earlier constructed a low-CpG pMBR2 plasmid vector supporting extended expression of transgenes in mesenchymal stem cells in vitro. Lengthy-term expression from the pMBR2 vector was studied for the wild-type mouse secreted alkaline phosphatase gene (mSEAPTwt) and its model devoid of CpGs (mSEAP0) after vector electroporation into mouse hindlimb muscle mass and hydrodynamic supply to the liver.
The mSEAP ranges within the blood had been measured over one yr. With the pMBR2-mSEAP0 assemble, the mSEAP ranges in leg muscle mass elevated greater than 2.5-fold within the first two months and remained increased than the preliminary stage till the top of the experiment. Far decrease expression ranges had been noticed with the management pCDNA3.1-mSEAP0 assemble. Expression from pMBR2-mSEAPwt decreased to about 40% after 6 months and remained at comparable ranges thereafter. Within the mouse liver, expression from pMBR2-mSEAP0 was roughly halved throughout the first 18 weeks after which lower slowly to the ultimate 17% stage.
Expression from pMBR2-mSEAPwt initially dropped to 18% and remained at roughly 10% thereafter. In distinction, expression from pCDNA3.1-mSEAP0 sharply dropped to five% after 2 weeks and remained at almost zero ranges all through the remainder of the experiment. Thus, each vector and transgene ought to have considerably diminished CpG contents to make sure extended plasmid-mediated expression within the liver, whereas minimizing the vector CpG content material is adequate for expression in skeletal muscle mass. The outcomes prompt moreover that the localization of S/MAR components throughout the transcription unit, in distinction to their outdoors location, ends in important discount of the extent of secreted, however not cytoplasmic, proteins.
Synthesis of aqueous AgInS/ZnS@PEI as a self-indicating nonviral vector for plasmid DNA self-tracking supply.
Varied nanoparticles have been employed for gene supply. Revealing the endocytosis or phagocytosis behaviors of the gene supply system is essential for designing refined gene therapies. Herein, a type of water-soluble PEI capped AgInS/ZnS quantum dot was synthesized as a self-indicating gene vector within the presence of branched polyethylenimine (PEI) and mercaptopropionic acid (MPA). The as-prepared ZAIS@PEI QDs might effectively condense plasmid DNA into nanocomplexes with robust fluorescence, which may reveal their positions throughout gene transfection.
) Goat anti Mouse IgG1 (Alexa Fluor 488) |
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| 43R-1649 | Fitzgerald | 500 ug | EUR 570 |
|
Description: Goat anti Mouse IgG1 secondary antibody (Alexa Fluor 488) |
|||
 Anti-Hu CD16 Alexa Fluor® 488 |
|||
| A4-646-T100 | ExBio | 100 tests | EUR 269 |
 AF488-streptavidin conjugate [Streptavidin, Alexa Fluor™ 488 Conjugate] |
|||
| 16891 | AAT Bioquest | 1 mg | EUR 176 |
 AF488 Phalloidin [equivalent to Alexa Fluor® 488 phalloidin] |
|||
| 23153 | AAT Bioquest | 300 Tests | EUR 306 |
 Anti-Hu CD72 Alexa Fluor® 488 |
|||
| A4-310-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Bov CD9 Alexa Fluor® 488 |
|||
| A4-354-C100 | ExBio | 0.1 mg | EUR 269 |
 Endoglin/CD105 Alexa Fluor |
|||
| FC15024 | Neuromics | 100 Tests | EUR 448 |
 Alexa Fluor 488–conjugated) Goat Anti-Mouse IgG(H+L) Alexa Fluor 488–conjugated |
|||
| S0017 | Affbiotech | 200ul | EUR 304 |
 Alexa Fluor 488–conjugated) Goat Anti-Rabbit IgG(H+L) Alexa Fluor 488–conjugated |
|||
| S0018 | Affbiotech | 200ul | EUR 304 |
) SAM FCM (Alexa Fluor 647) |
|||
| abx098902-100tests | Abbexa | 100 tests | EUR 1233 |
 Alexa Fluor<sup>®</sup> 488) Anti-Hu CD3 zeta (pY153) Alexa Fluor® 488 |
|||
| A4-686-C100 | ExBio | 0.1 mg | EUR 269 |
 Alexa Fluor<sup>®</sup> 488) Anti-Hu CD3 zeta (pY72) Alexa Fluor® 488 |
|||
| A4-712-C100 | ExBio | 0.1 mg | EUR 269 |
 Alexa Fluor<sup>®</sup> 488) Anti-Hu CD3 zeta (pY142) Alexa Fluor® 488 |
|||
| A4-730-C100 | ExBio | 0.1 mg | EUR 269 |
 Alexa Fluor<sup>®</sup> 488) Anti-Hu CD3 zeta (pY111) Alexa Fluor® 488 |
|||
| A4-737-C100 | ExBio | 0.1 mg | EUR 269 |
, Alexa Fluor® 488 Conjugated) Donkey Anti-Rabbit IgG (H+L), Alexa Fluor® 488 Conjugated |
|||
| Ab8032-001 | GenDepot | 0.5mg | EUR 435 |
 Monoclonal Antibody (104G4) (Alexa Fluor<sup>®</sup> 488)) Anti-LAMP3 (human) Monoclonal Antibody (104G4) (Alexa Fluor® 488) |
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| M09406 | BosterBio | 100ug | EUR 565 |
|
Description: Mouse Monoclonal LAMP3 (human) Antibody (104G4) (Alexa Fluor® 488). Validated in IHC and tested in Human. |
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) Anti-LAMP3 antibody (Alexa-fluor 546) |
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| STJ170005 | St John's Laboratory | 100 µg | EUR 393 |
|
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface. |
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) Anti-LAMP3 antibody (Alexa-fluor 647) |
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| STJ170006 | St John's Laboratory | 100 µg | EUR 393 |
|
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface. |
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) Anti-IL3RA antibody (Alexa-fluor 546) |
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| STJ170010 | St John's Laboratory | 100 µg | EUR 393 |
|
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment |
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) Anti-IL3RA antibody (Alexa-fluor 647) |
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| STJ170011 | St John's Laboratory | 100 µg | EUR 393 |
|
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment |
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) Anti-CD207 antibody (Alexa-fluor 546) |
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| STJ170015 | St John's Laboratory | 100 µg | EUR 393 |
|
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307) |
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) Anti-CD207 antibody (Alexa-fluor 647) |
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| STJ170016 | St John's Laboratory | 100 µg | EUR 393 |
|
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307) |
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) Anti-IL7R antibody (Alexa-fluor 546) |
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| STJ170021 | St John's Laboratory | 100 µg | EUR 393 |
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Description: The IL7-R consists of 2 chains, IL-7R |
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) Anti-IL7R antibody (Alexa-fluor 647) |
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| STJ170022 | St John's Laboratory | 100 µg | EUR 393 |
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Description: The IL7-R consists of 2 chains, IL-7R |
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 Alpha Fluor™ 488 amine |
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| 1705 | AAT Bioquest | 1 mg | EUR 306 |
 Alpha Fluor™ 488 Hydroxylamine |
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| 1900 | AAT Bioquest | 1 mg | EUR 306 |
 Monoclonal Antibody (DCGM4/122D5) (Alexa Fluor<sup>®</sup> 488)) Anti-Langerin (human) Monoclonal Antibody (DCGM4/122D5) (Alexa Fluor® 488) |
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| M02316 | BosterBio | 100ug | EUR 580 |
|
Description: Mouse Monoclonal Langerin (human) Antibody (DCGM4/122D5) (Alexa Fluor® 488). Validated in IHC and tested in Human. |
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 Alpha Fluor™ 488 NHS Ester |
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| 1812 | AAT Bioquest | 1 mg | EUR 219 |
 conjugate) Streptavidin-Alexa488 (Alexas fluor 488) conjugate |
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| SV-A488-100 | Alpha Diagnostics | 100 tests | EUR 225 |
-Alexa 488 Fluor conjugate (adsorbed with human IgG)) Rabbit Anti-Rat IgG (H+L)-Alexa 488 Fluor conjugate (adsorbed with human IgG) |
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| 50336 | Alpha Diagnostics | 0.5 ml | EUR 225 |
 Alpha Fluor™ 532 acid [equivalent to Alexa Fluor™ 532 acid] |
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| 1795 | AAT Bioquest | 10 mg | EUR 393 |
) Mouse IgG1-Alexa 488 conjugate (isotype control) |
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| 20102-101-A488 | Alpha Diagnostics | 50 Tests | EUR 263 |
 (Alexa Fluor 594)) Donkey anti Goat IgG (H + L) (Alexa Fluor 594) |
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| 43R-ID005AF | Fitzgerald | 500 ug | EUR 338 |
|
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 594) |
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 (Alexa Fluor 594)) Donkey anti Rat IgG (H + L) (Alexa Fluor 594) |
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| 43R-ID022AF | Fitzgerald | 500 ug | EUR 364 |
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Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594) |
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 (Alexa Fluor 647)) Donkey anti Goat IgG (H + L) (Alexa Fluor 647) |
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| 43R-ID028AF | Fitzgerald | 500 ug | EUR 430 |
|
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 647) |
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Donkey anti Rat IgG (H + L) (Alexa Fluor 594) |
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| 43R-ID047AF | Fitzgerald | 500 ug | EUR 462 |
|
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594) |
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 (Alexa Fluor 594)) Donkey anti Chicken IgY (H + L) (Alexa Fluor 594) |
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| 43R-ID056AF | Fitzgerald | 500 ug | EUR 343 |
|
Description: Donkey anti Chicken IgY secondary antibody (H + L) (Alexa Fluor 594) |
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 (Alexa Fluor 647)) Donkey anti Chicken IgY (H + L) (Alexa Fluor 647) |
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| 43R-ID060AF | Fitzgerald | 300 ug | EUR 425 |
|
Description: Donkey anti Chicken IgY (H + L) (Fab'2) (Alexa Fluor 647) |
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 (Alexa Fluor 594)) Rabbit anti Chicken IgY (H + L) (Alexa Fluor 594) |
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| 43R-IR016AF | Fitzgerald | 1 mg | EUR 281 |
|
Description: Rabbit anti Chicken IgY (H + L) secondary antibody (Alexa Fluor 594) |
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 AF350-streptavidin conjugate [Streptavidin, Alexa Fluor™ 350 Conjugate] |
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| 16890 | AAT Bioquest | 1 mg | EUR 176 |
 AF594-streptavidin conjugate [Streptavidin, Alexa Fluor™ 594 Conjugate] |
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| 16892 | AAT Bioquest | 1 mg | EUR 176 |
 AF350 Phalloidin [equivalent to Alexa Fluor® 350 phalloidin] |
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| 23150 | AAT Bioquest | 300 Tests | EUR 306 |
 AF594 Phalloidin [equivalent to Alexa Fluor® 594 phalloidin] |
|||
| 23158 | AAT Bioquest | 300 Tests | EUR 306 |
 Anti-Hu CD30 Alexa Fluor® 700 |
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| A7-455-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD94 Alexa Fluor® 700 |
|||
| A7-727-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD56 Alexa Fluor® 700 |
|||
| A7-789-T100 | ExBio | 100 tests | EUR 269 |
 Alexa Fluor 594–conjugated) Goat Anti-Mouse IgG(H+L) Alexa Fluor 594–conjugated |
|||
| S0005 | Affbiotech | 200ul | EUR 376 |
 Alexa Fluor 594–conjugated) Goat Anti-Rabbit IgG(H+L) Alexa Fluor 594–conjugated |
|||
| S0006 | Affbiotech | 200ul | EUR 376 |
 Alexa Fluor 647–conjugated) Goat Anti-Rabbit IgG(H+L) Alexa Fluor 647–conjugated |
|||
| S0013 | Affbiotech | 200ul | EUR 304 |
 Alexa Fluor 647–conjugated) Goat Anti-Mouse IgG(H+L) Alexa Fluor 647–conjugated |
|||
| S0014 | Affbiotech | 200ul | EUR 304 |
 Mouse pre-microRNA Expression Construct mir-488 |
|||
| MMIR-488-PA-1 | SBI | Bacterial Streak | EUR 684 |
 (Fab 2) (Alexa Fluor 594)) Donkey anti Goat IgG (H + L) (Fab 2) (Alexa Fluor 594) |
|||
| 43R-ID012AF | Fitzgerald | 300 ug | EUR 410 |
|
Description: Donkey anti Goat IgG (H + L) secondary antibody (Fab'2) (Alexa Fluor 594) |
|||
 Alexa Fluor<sup>®</sup> 647) Anti-Hu CD3 zeta (pY153) Alexa Fluor® 647 |
|||
| A6-686-C100 | ExBio | 0.1 mg | EUR 269 |
 Alexa Fluor<sup>®</sup> 647) Anti-Hu CD3 zeta (pY72) Alexa Fluor® 647 |
|||
| A6-712-C100 | ExBio | 0.1 mg | EUR 269 |
 Alexa Fluor<sup>®</sup> 647) Anti-Hu CD3 zeta (pY142) Alexa Fluor® 647 |
|||
| A6-730-C100 | ExBio | 0.1 mg | EUR 269 |
 Alexa Fluor<sup>®</sup> 647) Anti-Hu CD3 zeta (pY111) Alexa Fluor® 647 |
|||
| A6-737-C100 | ExBio | 0.1 mg | EUR 269 |
, Alexa Fluor® 594 Conjugated) Donkey Anti-Goat IgG (H+L), Alexa Fluor® 594 Conjugated |
|||
| Ab8011-001 | GenDepot | 1mg | EUR 334 |
-Alexa 594 Fluor conjugate (adsorbed with human IgG)) Rabbit Anti-Rat IgG (H+L)-Alexa 594 Fluor conjugate (adsorbed with human IgG) |
|||
| 50337 | Alpha Diagnostics | 0.5 ml | EUR 225 |
 Hepatitis C Virus (HCV) NS5 Genotype-2a ) Recombinant (E.Coli) Hepatitis C Virus (HCV) NS5 Genotype-2a |
|||
| RP-488 | Alpha Diagnostics | 100 ug | EUR 286 |
 Tide Fluor 2-LL-37 |
|||
| H-8286.0100 | Bachem | 0.1mg | EUR 312 |
|
Description: Sum Formula: C205H340N60O53+dye |
|||
Tide Fluor 2-LL-37 |
|||
| H-8286.0500 | Bachem | 0.5mg | EUR 1017 |
|
Description: Sum Formula: C205H340N60O53+dye |
|||
 Anti-Cytokeratins Alexa Fluor488 |
|||
| A4-108-C025 | ExBio | 0.025 mg | EUR 175 |
Anti-Cytokeratins Alexa Fluor488 |
|||
| A4-108-C100 | ExBio | 0.1 mg | EUR 310 |
 Anti-PSMA Alexa Fluor488 |
|||
| A4-539-C025 | ExBio | 0.025 mg | EUR 227 |
Anti-PSMA Alexa Fluor488 |
|||
| A4-539-C100 | ExBio | 0.1 mg | EUR 414 |
 Anti-FoxP3 Alexa Fluor488 |
|||
| A4-601-C025 | ExBio | 0.025 mg | EUR 201 |
Anti-FoxP3 Alexa Fluor488 |
|||
| A4-601-C100 | ExBio | 0.1 mg | EUR 362 |
 Anti-Phosphotyrosine Alexa Fluor647 |
|||
| A6-263-C025 | ExBio | 0.025 mg | EUR 154 |
Anti-Phosphotyrosine Alexa Fluor647 |
|||
| A6-263-C100 | ExBio | 0.1 mg | EUR 269 |
 Anti-LCK Alexa Fluor647 |
|||
| A6-269-C025 | ExBio | 0.025 mg | EUR 206 |
Anti-LCK Alexa Fluor647 |
|||
| A6-269-C100 | ExBio | 0.1 mg | EUR 373 |
 Anti-FoxP3 Alexa Fluor647 |
|||
| A6-601-C025 | ExBio | 0.025 mg | EUR 201 |
Anti-FoxP3 Alexa Fluor647 |
|||
| A6-601-C100 | ExBio | 0.1 mg | EUR 362 |
 Alpha Fluor™ 532 NHS Ester |
|||
| 1819 | AAT Bioquest | 1 mg | EUR 219 |
 Alpha Fluor™ 594 C5 Maleimide |
|||
| 1891 | AAT Bioquest | 1 mg | EUR 219 |
 Metal Fluor™ Zn-520, AM |
|||
| 21263 | AAT Bioquest | 1 mg | EUR 219 |
 Helix Fluor™ 545, succinimidyl ester |
|||
| 250 | AAT Bioquest | 1 mg | EUR 132 |
 Helix Fluor™ 575, succinimidyl ester |
|||
| 251 | AAT Bioquest | 1 mg | EUR 132 |
 Helix Fluor™ 6-JOE Phosphoramidite |
|||
| 6046 | AAT Bioquest | 100 umoles | EUR 50 |
) Tide Fluor 2-LL-37 (scrambled) |
|||
| H-8288.0100 | Bachem | 0.1mg | EUR 312 |
|
Description: Sum Formula: C205H340N60O53+dye |
|||
Tide Fluor 2-LL-37 (scrambled) |
|||
| H-8288.0500 | Bachem | 0.5mg | EUR 1017 |
|
Description: Sum Formula: C205H340N60O53+dye |
|||
 Tide Fluor 5WS-o-Conotoxin GVIA |
|||
| H-8356.0100 | Bachem | 0.1mg | EUR 1146 |
|
Description: Sum Formula: C120H182N38O43S6+dye |
|||
 conjugate) Streptavidin-Alexa594 (Alexas fluor 594) conjugate |
|||
| SV-A594-100 | Alpha Diagnostics | 100 tests | EUR 225 |
) Monoclonal Anti-Monkey IgG-Alexa 488 Conj. (specific for monkey; no reactivity with human or animals IgG) |
|||
| 70030-AF488 | Alpha Diagnostics | 50 tests | EUR 347 |
 Anti-Cytokeratin 18 Alexa Fluor488 |
|||
| A4-106-C025 | ExBio | 0.025 mg | EUR 186 |
Anti-Cytokeratin 18 Alexa Fluor488 |
|||
| A4-106-C100 | ExBio | 0.1 mg | EUR 331 |
 Anti-Cytokeratin 19 Alexa Fluor488 |
|||
| A4-120-C025 | ExBio | 0.025 mg | EUR 186 |
Anti-Cytokeratin 19 Alexa Fluor488 |
|||
| A4-120-C100 | ExBio | 0.1 mg | EUR 331 |
 Anti-Ki-67 Alexa Fluor488 |
|||
| A4-155-T025 | ExBio | 25 tests | EUR 154 |
Anti-Ki-67 Alexa Fluor488 |
|||
| A4-155-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD45 Alexa Fluor488 |
|||
| A4-160-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD193 Alexa Fluor488 |
|||
| A4-161-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD279 Alexa Fluor488 |
|||
| A4-176-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD43 Alexa Fluor488 |
|||
| A4-220-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD43 Alexa Fluor488 |
|||
| A4-220-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD44 Alexa Fluor488 |
|||
| A4-221-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD44 Alexa Fluor488 |
|||
| A4-221-T100 | ExBio | 100 tests | EUR 269 |
Anti-Hu CD45 Alexa Fluor488 |
|||
| A4-222-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD45 Alexa Fluor488 |
|||
| A4-222-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD55 Alexa Fluor488 |
|||
| A4-230-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD55 Alexa Fluor488 |
|||
| A4-230-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD50 Alexa Fluor488 |
|||
| A4-266-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD50 Alexa Fluor488 |
|||
| A4-266-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD31 Alexa Fluor488 |
|||
| A4-273-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD31 Alexa Fluor488 |
|||
| A4-273-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD147 Alexa Fluor488 |
|||
| A4-274-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD147 Alexa Fluor488 |
|||
| A4-274-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD34 Alexa Fluor488 |
|||
| A4-297-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD34 Alexa Fluor488 |
|||
| A4-297-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD105 Alexa Fluor488 |
|||
| A4-298-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD105 Alexa Fluor488 |
|||
| A4-298-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD41 Alexa Fluor488 |
|||
| A4-309-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD41 Alexa Fluor488 |
|||
| A4-309-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD72 Alexa Fluor488 |
|||
| A4-310-T025 | ExBio | 25 tests | EUR 154 |
 Anti-Hu CD63 Alexa Fluor488 |
|||
| A4-343-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD63 Alexa Fluor488 |
|||
| A4-343-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD13 Alexa Fluor488 |
|||
| A4-396-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD13 Alexa Fluor488 |
|||
| A4-396-T100 | ExBio | 100 tests | EUR 269 |
 Anti-HLA-G Alexa Fluor488 |
|||
| A4-431-C025 | ExBio | 0.025 mg | EUR 217 |
Anti-HLA-G Alexa Fluor488 |
|||
| A4-431-C100 | ExBio | 0.1 mg | EUR 394 |
Anti-HLA-G Alexa Fluor488 |
|||
| A4-437-C025 | ExBio | 0.025 mg | EUR 217 |
Anti-HLA-G Alexa Fluor488 |
|||
| A4-437-C100 | ExBio | 0.1 mg | EUR 394 |
 Anti-Hu CD300a Alexa Fluor488 |
|||
| A4-501-T100 | ExBio | 100 tests | EUR 269 |
 Anti-HLA-A2 Alexa Fluor488 |
|||
| A4-556-T025 | ExBio | 25 tests | EUR 154 |
Anti-HLA-A2 Alexa Fluor488 |
|||
| A4-556-T100 | ExBio | 100 tests | EUR 269 |
 Anti-CD3 zeta Alexa Fluor488 |
|||
| A4-568-C100 | ExBio | 0.1 mg | EUR 269 |
 Anti-Ms CD8a Alexa Fluor488 |
|||
| A4-579-C025 | ExBio | 0.025 mg | EUR 139 |
Anti-Ms CD8a Alexa Fluor488 |
|||
| A4-579-C100 | ExBio | 0.1 mg | EUR 238 |
 Anti-Hu CD326 Alexa Fluor488 |
|||
| A4-582-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD3 Alexa Fluor488 |
|||
| A4-631-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD16 Alexa Fluor488 |
|||
| A4-646-T025 | ExBio | 25 tests | EUR 154 |
 Anti-Hu CD150 Alexa Fluor488 |
|||
| A4-660-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD107a Alexa Fluor488 |
|||
| A4-671-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD107a Alexa Fluor488 |
|||
| A4-671-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD107b Alexa Fluor488 |
|||
| A4-672-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD107b Alexa Fluor488 |
|||
| A4-672-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD73 Alexa Fluor488 |
|||
| A4-675-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD11b Alexa Fluor488 |
|||
| A4-681-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD11b Alexa Fluor488 |
|||
| A4-681-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD35 Alexa Fluor488 |
|||
| A4-703-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD305 Alexa Fluor488 |
|||
| A4-713-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD161 Alexa Fluor488 |
|||
| A4-729-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD144 Alexa Fluor488 |
|||
| A4-770-T100 | ExBio | 100 tests | EUR 269 |
 Anti-HLA-ABCE Alexa Fluor488 |
|||
| A4-813-C100 | ExBio | 0.1 mg | EUR 373 |
 Anti-Ki-67 Alexa Fluor647 |
|||
| A6-155-T025 | ExBio | 25 tests | EUR 154 |
Anti-Ki-67 Alexa Fluor647 |
|||
| A6-155-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD45 Alexa Fluor647 |
|||
| A6-160-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD193 Alexa Fluor647 |
|||
| A6-161-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD279 Alexa Fluor647 |
|||
| A6-176-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD273 Alexa Fluor647 |
|||
| A6-178-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD231 Alexa Fluor647 |
|||
| A6-200-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD9 Alexa Fluor647 |
|||
| A6-208-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD9 Alexa Fluor647 |
|||
| A6-208-T100 | ExBio | 100 tests | EUR 269 |
Anti-Hu CD45 Alexa Fluor647 |
|||
| A6-222-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD45 Alexa Fluor647 |
|||
| A6-222-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD45RB Alexa Fluor647 |
|||
| A6-224-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD45RB Alexa Fluor647 |
|||
| A6-224-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD55 Alexa Fluor647 |
|||
| A6-230-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD55 Alexa Fluor647 |
|||
| A6-230-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD59 Alexa Fluor647 |
|||
| A6-233-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD59 Alexa Fluor647 |
|||
| A6-233-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD71 Alexa Fluor647 |
|||
| A6-235-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD71 Alexa Fluor647 |
|||
| A6-235-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD21 Alexa Fluor647 |
|||
| A6-306-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD21 Alexa Fluor647 |
|||
| A6-306-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD177 Alexa Fluor647 |
|||
| A6-314-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD177 Alexa Fluor647 |
|||
| A6-314-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD222 Alexa Fluor647 |
|||
| A6-315-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD222 Alexa Fluor647 |
|||
| A6-315-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD30 Alexa Fluor647 |
|||
| A6-455-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD30 Alexa Fluor647 |
|||
| A6-455-T100 | ExBio | 100 tests | EUR 269 |
 Anti-gamma-Tubulin Alexa Fluor647 |
|||
| A6-465-C025 | ExBio | 0.025 mg | EUR 217 |
Anti-gamma-Tubulin Alexa Fluor647 |
|||
| A6-465-C100 | ExBio | 0.1 mg | EUR 394 |
 Anti-Hu CD264 Alexa Fluor647 |
|||
| A6-519-C100 | ExBio | 0.1 mg | EUR 269 |
 Anti-Hu CD69 Alexa Fluor647 |
|||
| A6-552-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD69 Alexa Fluor647 |
|||
| A6-552-T100 | ExBio | 100 tests | EUR 269 |
 Anti-HLA-A2 Alexa Fluor647 |
|||
| A6-556-T025 | ExBio | 25 tests | EUR 154 |
Anti-HLA-A2 Alexa Fluor647 |
|||
| A6-556-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD326 Alexa Fluor647 |
|||
| A6-581-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD326 Alexa Fluor647 |
|||
| A6-581-T100 | ExBio | 100 tests | EUR 269 |
Anti-Hu CD326 Alexa Fluor647 |
|||
| A6-582-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD158d Alexa Fluor647 |
|||
| A6-609-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD3 Alexa Fluor647 |
|||
| A6-631-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD209 Alexa Fluor647 |
|||
| A6-640-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD64 Alexa Fluor647 |
|||
| A6-644-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD64 Alexa Fluor647 |
|||
| A6-644-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD16 Alexa Fluor647 |
|||
| A6-646-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD16 Alexa Fluor647 |
|||
| A6-646-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD150 Alexa Fluor647 |
|||
| A6-660-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD73 Alexa Fluor647 |
|||
| A6-675-T100 | ExBio | 100 tests | EUR 269 |
 Anti-HLA-DR Alexa Fluor647 |
|||
| A6-690-T025 | ExBio | 25 tests | EUR 154 |
Anti-HLA-DR Alexa Fluor647 |
|||
| A6-690-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD161 Alexa Fluor647 |
|||
| A6-729-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD200 Alexa Fluor647 |
|||
| A6-746-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD160 Alexa Fluor647 |
|||
| A6-750-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD243 Alexa Fluor647 |
|||
| A6-764-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD144 Alexa Fluor647 |
|||
| A6-770-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD206 Alexa Fluor647 |
|||
| A6-782-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD152 Alexa Fluor647 |
|||
| A6-785-T100 | ExBio | 100 tests | EUR 269 |
Our outcomes confirmed that ZAIS@PEI might mediate plasmid DNA supply into HeLa cells with the excessive transfection effectivity of 40% and low cytotoxicity, in the meantime permitting the real-time monitoring of gene transfection. The obtained ZAIS@PEI QDs had been verified as versatile self-tracking gene vectors, which built-in gene supply with bioimaging capabilities with out exterior labeling.