Successful delivery of large-size CRISPR/Cas9 vectors in hard-to-transfect human cells using small plasmids

Successful delivery of large-size CRISPR/Cas9 vectors in hard-to-transfect human cells using small plasmids

With the rise of recent highly effective genome engineering applied sciences, equivalent to CRISPR/Cas9, cell fashions might be engineered successfully to speed up fundamental and illness analysis. Essentially the most essential step on this process is the environment friendly supply of international nucleic acids into cells by mobile transfection. For the reason that vectors encoding the elements crucial for CRISPR/Cas genome engineering are at all times massive (9-19 kb), they end in low transfection effectivity and cell viability, and thus subsequent choice or purification of optimistic cells is required.

To beat these obstacles, we right here present a non-toxic and non-viral supply methodology that will increase transfection effectivity (as much as 40-fold) and cell viability (as much as 6-fold) in plenty of hard-to-transfect human most cancers cell strains and first blood cells. At its core, the approach is predicated on including exogenous small plasmids of an outlined measurement to the transfection combination. The “entire genome” TMV-based expression system, Geneware®, was used within the cGMP manufacturing of the plant-made pharmaceutical Q-Griffithsin and demonstrates steady expression for as much as a two-year interval.

Virion and plasmid banks which contained viral cDNA and a Q-Griffithsin sequence had been capable of produce >200 g of Q-Griffithsin. Knowledge assessing the standard and stability of the product banks had been measured by means of useful assessments of visible symptomology and product expression.

The cell-penetrating YopM protein-functionalized quantum dot-plasmid DNA conjugate as a novel gene supply vector

Non-viral gene supply techniques have nice potential for secure and environment friendly gene remedy, whereas inefficient mobile and nuclear uptake stay as the foremost hurdles. Novel approaches are wanted to reinforce the transfection effectivity of non-viral vectors. In accordance with this want, the target of this examine was to assemble a non-viral vector that might obtain gene supply with out utilizing further lipid-based transfection agent. We aimed to impart self-delivery property to a non-viral vector through the use of the cell and nucleus penetrating properties of YopM proteins from the three Yersinia spp. (Y. pestis, Y. enterocolotica and Y. pseudotuberculosis).

Plasmid DNA (pDNA) encoding inexperienced fluorescent protein (GFP) was labeled with quantum dots (QDs) through peptide-nucleic acid (PNA) recognition web site. Recombinant YopM protein was then hooked up to the conjugate through a second PNA recognition web site. The YopM ̶ QDs ̶ pDNA conjugate was transfected into HeLa cells with out utilizing further transfection reagent. All three conjugates produced GFP fluorescence, indicating that the plasmid was efficiently delivered to the nucleus.

As management, bare pDNA was transfected into the cells through the use of a industrial transfection reagent. The Y. pseudotuberculosis YopM-functionalized conjugate achieved the best GFP expression, in comparison with different two YopM proteins and the transfection reagent. To one of the best of our data, YopM protein was used for the primary time in a non-viral gene supply vector.

[Maintenance of Plasmid Expression in vivo Depends Primarily on the CpG Contents of the Vector and Transgene]

Plasmid-mediated gene remedy, being a secure and comparatively cheap therapeutic technique, is affected by a quick silencing of transgene expression. The silencing severely reduces the long-term effectivity of plasmid vectors. Now we have earlier constructed a low-CpG pMBR2 plasmid vector supporting extended expression of transgenes in mesenchymal stem cells in vitro. Lengthy-term expression from the pMBR2 vector was studied for the wild-type mouse secreted alkaline phosphatase gene (mSEAPTwt) and its model devoid of CpGs (mSEAP0) after vector electroporation into mouse hindlimb muscle mass and hydrodynamic supply to the liver.

Successful delivery of large-size CRISPR/Cas9 vectors in hard-to-transfect human cells using small plasmids

The mSEAP ranges within the blood had been measured over one yr. With the pMBR2-mSEAP0 assemble, the mSEAP ranges in leg muscle mass elevated greater than 2.5-fold within the first two months and remained increased than the preliminary stage till the top of the experiment. Far decrease expression ranges had been noticed with the management pCDNA3.1-mSEAP0 assemble. Expression from pMBR2-mSEAPwt decreased to about 40% after 6 months and remained at comparable ranges thereafter. Within the mouse liver, expression from pMBR2-mSEAP0 was roughly halved throughout the first 18 weeks after which lower slowly to the ultimate 17% stage.

Expression from pMBR2-mSEAPwt initially dropped to 18% and remained at roughly 10% thereafter. In distinction, expression from pCDNA3.1-mSEAP0 sharply dropped to five% after 2 weeks and remained at almost zero ranges all through the remainder of the experiment. Thus, each vector and transgene ought to have considerably diminished CpG contents to make sure extended plasmid-mediated expression within the liver, whereas minimizing the vector CpG content material is adequate for expression in skeletal muscle mass. The outcomes prompt moreover that the localization of S/MAR components throughout the transcription unit, in distinction to their outdoors location, ends in important discount of the extent of secreted, however not cytoplasmic, proteins.

Synthesis of aqueous AgInS/ZnS@PEI as a self-indicating nonviral vector for plasmid DNA self-tracking supply.

Varied nanoparticles have been employed for gene supply. Revealing the endocytosis or phagocytosis behaviors of the gene supply system is essential for designing refined gene therapies. Herein, a type of water-soluble PEI capped AgInS/ZnS quantum dot was synthesized as a self-indicating gene vector within the presence of branched polyethylenimine (PEI) and mercaptopropionic acid (MPA). The as-prepared ZAIS@PEI QDs might effectively condense plasmid DNA into nanocomplexes with robust fluorescence, which may reveal their positions throughout gene transfection.

Alpha Fluor™ 488 acid [equivalent to Alexa Fluor™ 488 acid]

1790 5 mg
EUR 367.2

Goat anti Mouse IgG1 (Alexa Fluor 488)

43R-1649 500 ug
EUR 684
Description: Goat anti Mouse IgG1 secondary antibody (Alexa Fluor 488)

Anti-Hu CD16 Alexa Fluor® 488

A4-646-T100 100 tests
EUR 322.8

AF488-streptavidin conjugate [Streptavidin, Alexa Fluor™ 488 Conjugate]

16891 1 mg
EUR 211.2

AF488 Phalloidin [equivalent to Alexa Fluor® 488 phalloidin]

23153 300 Tests
EUR 367.2

Anti-Hu CD72 Alexa Fluor® 488

A4-310-T100 100 tests
EUR 322.8

Anti-Bov CD9 Alexa Fluor® 488

A4-354-C100 0.1 mg
EUR 322.8

XFD488 tetrazine *Same Structure to Alexa Fluor™ 488 tetrazine*

4600 1 mg
EUR 157

XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde*

9015 5 mg
EUR 425

XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde*

9016 1 mg
EUR 308

iFluor® 488 Styramide *Superior Replacement for Alexa Fluor 488 tyramide and Opal 520*

45020 100 Slides
EUR 334

Anti-Hu CD3 zeta (pY153) Alexa Fluor® 488

A4-686-C100 0.1 mg
EUR 322.8

Anti-Hu CD3 zeta (pY72) Alexa Fluor® 488

A4-712-C100 0.1 mg
EUR 322.8

Anti-Hu CD3 zeta (pY142) Alexa Fluor® 488

A4-730-C100 0.1 mg
EUR 322.8

Anti-Hu CD3 zeta (pY111) Alexa Fluor® 488

A4-737-C100 0.1 mg
EUR 322.8

Donkey Anti-Rabbit IgG (H+L), Alexa Fluor® 488 Conjugated

Ab8032-001 0.5mg
EUR 522

XFD488 tyramide reagent *Same Structure to Alexa Fluor™ 488 tyramide*

11070 200 slides
EUR 222

Alpha Fluor™ 488 amine

1705 1 mg
EUR 367.2

Alpha Fluor™ 488 Hydroxylamine

1900 1 mg
EUR 367.2

Alpha Fluor™ 488 alkyne

1700 1 mg
EUR 367.2

Endoglin/CD105 Alexa Fluor

FC15024 100 Tests
EUR 537.6

Anti-LAMP3 (human) Monoclonal Antibody (104G4) (Alexa Fluor® 488)

M09406 100ug
EUR 678
Description: Mouse Monoclonal LAMP3 (human) Antibody (104G4) (Alexa Fluor® 488). Validated in IHC and tested in Human.

Concanavalin A, XFD488 Labeled *XFD488 Same Structure to Alexa Fluor™ 488*

25570 1 mg
EUR 102

XFD488 NHS Ester *Same Structure to Alexa Fluor™ 488 NHS Ester*

71902 5 mg
EUR 781

Mouse pre-microRNA Expression Construct mir-488

MMIR-488-PA-1 Bacterial Streak
EUR 820.8

Anti-Langerin (human) Monoclonal Antibody (DCGM4/122D5) (Alexa Fluor® 488)

M02316 100ug
EUR 696
Description: Mouse Monoclonal Langerin (human) Antibody (DCGM4/122D5) (Alexa Fluor® 488). Validated in IHC and tested in Human.

XFD488 NHS Ester-UltraPure Grade *XFD488 Same Structure to Alexa Fluor™ 488*

1810 1 mg
EUR 278

Wheat Germ Agglutinin, XFD488 Labeled *XFD488 Same Structure to Alexa Fluor™ 488*

25500 1 mg
EUR 102

XFD488 NHS Ester-UltraPure Grade *XFD488 Same Structure to Alexa Fluor™ 488*

71901 5 mg
EUR 989

Bodi Fluor™ 488-Cephaeline Conjugate

32000 1 mg
EUR 784.8

Alpha Fluor™ 488 NHS Ester

1812 1 mg
EUR 262.8

Streptavidin-Alexa488 (Alexas fluor 488) conjugate

SV-A488-100 100 tests
EUR 270

SAM FCM (Alexa Fluor 647)

abx098902-100tests 100 tests
EUR 1479.6

Rabbit Anti-Rat IgG (H+L)-Alexa 488 Fluor conjugate (adsorbed with human IgG)

50336 0.5 ml
EUR 270

XFD488 Anti-human CD47 Antibody *HI172, XFD488 Same Structure to Alexa Fluor™ 488*

10471150 100 tests
EUR 245

XFD488 Anti-human CD47 Antibody *HI172, XFD488 Same Structure to Alexa Fluor™ 488*

10471151 500 tests
EUR 918

XFD488 Anti-human CD52 Antibody *HI186, XFD488 Same Structure to Alexa Fluor™ 488*

10520150 100 tests
EUR 245

XFD488 Anti-human CD52 Antibody *HI186, XFD488 Same Structure to Alexa Fluor™ 488*

10520151 500 tests
EUR 918

XFD488 Anti-human CD53 Antibody *HI29, XFD488 Same Structure to Alexa Fluor™ 488*

10530150 100 tests
EUR 245

XFD488 Anti-human CD53 Antibody *HI29, XFD488 Same Structure to Alexa Fluor™ 488*

10530151 500 tests
EUR 918

XFD488 Anti-human CD53 Antibody *HI36, XFD488 Same Structure to Alexa Fluor™ 488*

10531150 100 tests
EUR 245

XFD488 Anti-human CD53 Antibody *HI36, XFD488 Same Structure to Alexa Fluor™ 488*

10531151 500 tests
EUR 918

XFD488 Anti-human CD55 Antibody *HI55a, XFD488 Same Structure to Alexa Fluor™ 488*

10550150 100 tests
EUR 245

XFD488 Anti-human CD55 Antibody *HI55a, XFD488 Same Structure to Alexa Fluor™ 488*

10550151 500 tests
EUR 918

XFD488 Anti-human CD56 Antibody *My31, XFD488 Same Structure to Alexa Fluor™ 488*

10562140 100 tests
EUR 245

XFD488 Anti-human CD56 Antibody *My31, XFD488 Same Structure to Alexa Fluor™ 488*

10562141 500 tests
EUR 918

XFD488 Anti-human CD57 Antibody *HI57a, XFD488 Same Structure to Alexa Fluor™ 488*

10570150 100 tests
EUR 245

XFD488 Anti-human CD57 Antibody *HI57a, XFD488 Same Structure to Alexa Fluor™ 488*

10570151 500 tests
EUR 918

XFD488 Anti-human CD58 Antibody *HI58a, XFD488 Same Structure to Alexa Fluor™ 488*

10580150 100 tests
EUR 245

XFD488 Anti-human CD58 Antibody *HI58a, XFD488 Same Structure to Alexa Fluor™ 488*

10580151 500 tests
EUR 918

XFD488 Anti-human CD62 Antibody *HI62E, XFD488 Same Structure to Alexa Fluor™ 488*

10620150 100 tests
EUR 245

XFD488 Anti-human CD62 Antibody *HI62E, XFD488 Same Structure to Alexa Fluor™ 488*

10620151 500 tests
EUR 918

XFD488 Anti-human CD62l Antibody *HI62L, XFD488 Same Structure to Alexa Fluor™ 488*

10621150 100 tests
EUR 245

XFD488 Anti-human CD62l Antibody *HI62L, XFD488 Same Structure to Alexa Fluor™ 488*

10621151 500 tests
EUR 918

XFD488 Anti-human CD62p Antibody *HI62P, XFD488 Same Structure to Alexa Fluor™ 488*

10622150 100 tests
EUR 245

XFD488 Anti-human CD62p Antibody *HI62P, XFD488 Same Structure to Alexa Fluor™ 488*

10622151 500 tests
EUR 918

XFD488 Anti-human CD64 Antibody *10.1, XFD488 Same Structure to Alexa Fluor™ 488*

10640150 100 tests
EUR 245

XFD488 Anti-human CD64 Antibody *10.1, XFD488 Same Structure to Alexa Fluor™ 488*

10640151 500 tests
EUR 918

XFD488 Anti-human CD69 Antibody *FN50, XFD488 Same Structure to Alexa Fluor™ 488*

10690150 100 tests
EUR 547

XFD488 Anti-human CD69 Antibody *FN50, XFD488 Same Structure to Alexa Fluor™ 488*

10690151 500 tests
EUR 2051

XFD488 Anti-human CD71 Antibody *HI160, XFD488 Same Structure to Alexa Fluor™ 488*

10710150 100 tests
EUR 245

XFD488 Anti-human CD71 Antibody *HI160, XFD488 Same Structure to Alexa Fluor™ 488*

10710151 500 tests
EUR 918

XFD488 Anti-human CD71 Antibody *HI166, XFD488 Same Structure to Alexa Fluor™ 488*

10711150 100 tests
EUR 245

XFD488 Anti-human CD71 Antibody *HI166, XFD488 Same Structure to Alexa Fluor™ 488*

10711151 500 tests
EUR 918

XFD488 Anti-human CD72 Antibody *3F3, XFD488 Same Structure to Alexa Fluor™ 488*

10720150 100 tests
EUR 547

XFD488 Anti-human CD72 Antibody *3F3, XFD488 Same Structure to Alexa Fluor™ 488*

10720151 500 tests
EUR 2051

XFD488 Anti-human CD73 Antibody *AD2, XFD488 Same Structure to Alexa Fluor™ 488*

10730150 100 tests
EUR 547

XFD488 Anti-human CD73 Antibody *AD2, XFD488 Same Structure to Alexa Fluor™ 488*

10730151 500 tests
EUR 2051

XFD488 Anti-human CD5 Antibody *HISM2, XFD488 Same Structure to Alexa Fluor™ 488*

10050150 100 tests
EUR 245

XFD488 Anti-human CD5 Antibody *HISM2, XFD488 Same Structure to Alexa Fluor™ 488*

10050151 500 tests
EUR 918

XFD488 Anti-human CD5 Antibody *L17F12, XFD488 Same Structure to Alexa Fluor™ 488*

10051150 100 tests
EUR 245

XFD488 Anti-human CD5 Antibody *L17F12, XFD488 Same Structure to Alexa Fluor™ 488*

10051151 500 tests
EUR 918

XFD488 Anti-human CD5 Antibody *UCHT2, XFD488 Same Structure to Alexa Fluor™ 488*

10052150 100 tests
EUR 245

XFD488 Anti-human CD5 Antibody *UCHT2, XFD488 Same Structure to Alexa Fluor™ 488*

10052151 500 tests
EUR 918

XFD488 Anti-human CD6 Antibody *HI210, XFD488 Same Structure to Alexa Fluor™ 488*

10060150 100 tests
EUR 245

XFD488 Anti-human CD6 Antibody *HI210, XFD488 Same Structure to Alexa Fluor™ 488*

10060151 500 tests
EUR 918

XFD488 Anti-human CD7 Antibody *HIT7, XFD488 Same Structure to Alexa Fluor™ 488*

10070150 100 tests
EUR 245

XFD488 Anti-human CD7 Antibody *HIT7, XFD488 Same Structure to Alexa Fluor™ 488*

10070151 500 tests
EUR 918

XFD488 Anti-human CD8 Antibody *HIT8a, XFD488 Same Structure to Alexa Fluor™ 488*

10080150 100 tests
EUR 245

XFD488 Anti-human CD8 Antibody *HIT8a, XFD488 Same Structure to Alexa Fluor™ 488*

10080151 500 tests
EUR 918

XFD488 Anti-human CD8 Antibody *SK1, XFD488 Same Structure to Alexa Fluor™ 488*

10081150 100 tests
EUR 245

XFD488 Anti-human CD8 Antibody *SK1, XFD488 Same Structure to Alexa Fluor™ 488*

10081151 500 tests
EUR 918

XFD488 Anti-human CD9 Antibody *HI9a, XFD488 Same Structure to Alexa Fluor™ 488*

10090150 100 tests
EUR 245

XFD488 Anti-human CD9 Antibody *HI9a, XFD488 Same Structure to Alexa Fluor™ 488*

10090151 500 tests
EUR 918

XFD488 Anti-human CD10 Antibody *HI10a, XFD488 Same Structure to Alexa Fluor™ 488*

10100150 100 tests
EUR 245

XFD488 Anti-human CD10 Antibody *HI10a, XFD488 Same Structure to Alexa Fluor™ 488*

10100151 500 tests
EUR 918

XFD488 Anti-human CD11a Antibody *HI111, XFD488 Same Structure to Alexa Fluor™ 488*

10110150 100 tests
EUR 245

XFD488 Anti-human CD11a Antibody *HI111, XFD488 Same Structure to Alexa Fluor™ 488*

10110151 500 tests
EUR 918

XFD488 Anti-human CD11b Antibody *HI11b, XFD488 Same Structure to Alexa Fluor™ 488*

10111150 100 tests
EUR 245

XFD488 Anti-human CD11b Antibody *HI11b, XFD488 Same Structure to Alexa Fluor™ 488*

10111151 500 tests
EUR 918

XFD488 Anti-human CD11b Antibody *ICRF44, XFD488 Same Structure to Alexa Fluor™ 488*

10112150 100 tests
EUR 245

XFD488 Anti-human CD11b Antibody *ICRF44, XFD488 Same Structure to Alexa Fluor™ 488*

10112151 500 tests
EUR 918

XFD488 Anti-human CD11c Antibody *3.9, XFD488 Same Structure to Alexa Fluor™ 488*

10113150 100 tests
EUR 245

XFD488 Anti-human CD11c Antibody *3.9, XFD488 Same Structure to Alexa Fluor™ 488*

10113151 500 tests
EUR 918

XFD488 Anti-human CD13 Antibody *WM15, XFD488 Same Structure to Alexa Fluor™ 488*

10130150 100 tests
EUR 245

XFD488 Anti-human CD13 Antibody *WM15, XFD488 Same Structure to Alexa Fluor™ 488*

10130151 500 tests
EUR 918

XFD488 Anti-human CD14 Antibody *61D3, XFD488 Same Structure to Alexa Fluor™ 488*

10141150 100 tests
EUR 245

XFD488 Anti-human CD14 Antibody *61D3, XFD488 Same Structure to Alexa Fluor™ 488*

10141151 500 tests
EUR 918

XFD488 Anti-human CD15 Antibody *HI98, XFD488 Same Structure to Alexa Fluor™ 488*

10150150 100 tests
EUR 296

XFD488 Anti-human CD15 Antibody *HI98, XFD488 Same Structure to Alexa Fluor™ 488*

10150151 500 tests
EUR 1020

XFD488 Anti-human CD16 Antibody *HI16a, XFD488 Same Structure to Alexa Fluor™ 488*

10160150 100 tests
EUR 245

XFD488 Anti-human CD16 Antibody *HI16a, XFD488 Same Structure to Alexa Fluor™ 488*

10160151 500 tests
EUR 918

XFD488 Anti-human CD16 Antibody *3G8, XFD488 Same Structure to Alexa Fluor™ 488*

10161150 100 tests
EUR 245

XFD488 Anti-human CD16 Antibody *3G8, XFD488 Same Structure to Alexa Fluor™ 488*

10161151 500 tests
EUR 918

XFD488 Anti-human CD18 Antibody *HI18a, XFD488 Same Structure to Alexa Fluor™ 488*

10180150 100 tests
EUR 245

XFD488 Anti-human CD18 Antibody *HI18a, XFD488 Same Structure to Alexa Fluor™ 488*

10180151 500 tests
EUR 918

XFD488 Anti-human CD19 Antibody *HI19a, XFD488 Same Structure to Alexa Fluor™ 488*

10190150 100 tests
EUR 245

XFD488 Anti-human CD19 Antibody *HI19a, XFD488 Same Structure to Alexa Fluor™ 488*

10190151 500 tests
EUR 918

XFD488 Anti-human CD19 Antibody *SJ25C1, XFD488 Same Structure to Alexa Fluor™ 488*

10191150 100 tests
EUR 245

XFD488 Anti-human CD19 Antibody *SJ25C1, XFD488 Same Structure to Alexa Fluor™ 488*

10191151 500 tests
EUR 918

XFD488 Anti-human CD19 Antibody *HIB19, XFD488 Same Structure to Alexa Fluor™ 488*

10192150 100 tests
EUR 245

XFD488 Anti-human CD19 Antibody *HIB19, XFD488 Same Structure to Alexa Fluor™ 488*

10192151 500 tests
EUR 918

XFD488 Anti-human CD19 Antibody *4G7, XFD488 Same Structure to Alexa Fluor™ 488*

10193150 100 tests
EUR 245

XFD488 Anti-human CD19 Antibody *4G7, XFD488 Same Structure to Alexa Fluor™ 488*

10193151 500 tests
EUR 918

XFD488 Anti-mouse CD19 Antibody *1D3, XFD488 Same Structure to Alexa Fluor™ 488*

10194140 100 tests
EUR 245

XFD488 Anti-mouse CD19 Antibody *1D3, XFD488 Same Structure to Alexa Fluor™ 488*

10194141 500 tests
EUR 918

XFD488 Anti-human CD20 Antibody *HI20a, XFD488 Same Structure to Alexa Fluor™ 488*

10201150 100 tests
EUR 245

XFD488 Anti-human CD20 Antibody *HI20a, XFD488 Same Structure to Alexa Fluor™ 488*

10201151 500 tests
EUR 918

XFD488 Anti-human CD21 Antibody *HI21a, XFD488 Same Structure to Alexa Fluor™ 488*

10210150 100 tests
EUR 245

XFD488 Anti-human CD21 Antibody *HI21a, XFD488 Same Structure to Alexa Fluor™ 488*

10210151 500 tests
EUR 918

XFD488 Anti-human CD22 Antibody *HIB22, XFD488 Same Structure to Alexa Fluor™ 488*

10220150 100 tests
EUR 245

XFD488 Anti-human CD22 Antibody *HIB22, XFD488 Same Structure to Alexa Fluor™ 488*

10220151 500 tests
EUR 918

XFD488 Anti-human CD101 Antibody *BB27, XFD488 Same Structure to Alexa Fluor™ 488*

11010140 100 tests
EUR 547

XFD488 Anti-human CD101 Antibody *BB27, XFD488 Same Structure to Alexa Fluor™ 488*

11010141 500 tests
EUR 2051

XFD488 Anti-human CD107 Antibody *H4B4, XFD488 Same Structure to Alexa Fluor™ 488*

11071140 100 tests
EUR 547

XFD488 Anti-human CD107 Antibody *H4B4, XFD488 Same Structure to Alexa Fluor™ 488*

11071141 500 tests
EUR 2051

XFD488 Anti-human CD109 Antibody *W7C5, XFD488 Same Structure to Alexa Fluor™ 488*

11090140 100 tests
EUR 547

XFD488 Anti-human CD109 Antibody *W7C5, XFD488 Same Structure to Alexa Fluor™ 488*

11090141 500 tests
EUR 2051

XFD488 Anti-human CD111 Antibody *R1.302, XFD488 Same Structure to Alexa Fluor™ 488*

11110140 100 tests
EUR 547

XFD488 Anti-human CD111 Antibody *R1.302, XFD488 Same Structure to Alexa Fluor™ 488*

11110141 500 tests
EUR 2051

XFD488 Anti-human CD112 Antibody *R2.525, XFD488 Same Structure to Alexa Fluor™ 488*

11120140 100 tests
EUR 547

XFD488 Anti-human CD112 Antibody *R2.525, XFD488 Same Structure to Alexa Fluor™ 488*

11120141 500 tests
EUR 2051

XFD488 Anti-human CD114 Antibody *LMM741, XFD488 Same Structure to Alexa Fluor™ 488*

11140140 100 tests
EUR 547

XFD488 Anti-human CD114 Antibody *LMM741, XFD488 Same Structure to Alexa Fluor™ 488*

11140141 500 tests
EUR 2051

XFD488 Anti-human CD116 Antibody *4H1, XFD488 Same Structure to Alexa Fluor™ 488*

11160140 100 tests
EUR 547

XFD488 Anti-human CD116 Antibody *4H1, XFD488 Same Structure to Alexa Fluor™ 488*

11160141 500 tests
EUR 2051

XFD488 Anti-human CD117 Antibody *104D2, XFD488 Same Structure to Alexa Fluor™ 488*

11170150 100 tests
EUR 547

XFD488 Anti-human CD117 Antibody *104D2, XFD488 Same Structure to Alexa Fluor™ 488*

11170151 500 tests
EUR 2051

XFD488 Anti-human CD120a Antibody *H398, XFD488 Same Structure to Alexa Fluor™ 488*

11200140 100 tests
EUR 547

XFD488 Anti-human CD120a Antibody *H398, XFD488 Same Structure to Alexa Fluor™ 488*

11200141 500 tests
EUR 2051

XFD488 Anti-human CD122 Antibody *TU27, XFD488 Same Structure to Alexa Fluor™ 488*

11220140 100 tests
EUR 547

XFD488 Anti-human CD122 Antibody *TU27, XFD488 Same Structure to Alexa Fluor™ 488*

11220141 500 tests
EUR 2051

XFD488 Anti-human CD123 Antibody *6H6, XFD488 Same Structure to Alexa Fluor™ 488*

11230150 100 tests
EUR 157

XFD488 Anti-human CD123 Antibody *6H6, XFD488 Same Structure to Alexa Fluor™ 488*

11230151 500 tests
EUR 484

XFD488 Anti-human CD123 Antibody *12H7, XFD488 Same Structure to Alexa Fluor™ 488*

11231140 100 tests
EUR 245

XFD488 Anti-human CD123 Antibody *12H7, XFD488 Same Structure to Alexa Fluor™ 488*

11231141 500 tests
EUR 918

XFD488 Anti-human CD133 Antibody *293C3, XFD488 Same Structure to Alexa Fluor™ 488*

11330140 100 tests
EUR 607

XFD488 Anti-human CD133 Antibody *293C3, XFD488 Same Structure to Alexa Fluor™ 488*

11330141 500 tests
EUR 2280

XFD488 Anti-human CD135 Antibody *BV10A4, XFD488 Same Structure to Alexa Fluor™ 488*

11350140 100 tests
EUR 547

XFD488 Anti-human CD135 Antibody *BV10A4, XFD488 Same Structure to Alexa Fluor™ 488*

11350141 500 tests
EUR 2051

XFD488 Anti-human CD138 Antibody *MI15, XFD488 Same Structure to Alexa Fluor™ 488*

11380150 100 tests
EUR 245

XFD488 Anti-human CD138 Antibody *MI15, XFD488 Same Structure to Alexa Fluor™ 488*

11380151 500 tests
EUR 918

XFD488 Anti-human CD140a Antibody *16A1, XFD488 Same Structure to Alexa Fluor™ 488*

11400140 100 tests
EUR 547

XFD488 Anti-human CD140a Antibody *16A1, XFD488 Same Structure to Alexa Fluor™ 488*

11400141 500 tests
EUR 2051

XFD488 Anti-human CD140b Antibody *18A2, XFD488 Same Structure to Alexa Fluor™ 488*

11401140 100 tests
EUR 547

XFD488 Anti-human CD140b Antibody *18A2, XFD488 Same Structure to Alexa Fluor™ 488*

11401141 500 tests
EUR 2051

XFD488 Anti-human CD24 Antibody *HI45, XFD488 Same Structure to Alexa Fluor™ 488*

10240150 100 tests
EUR 245

XFD488 Anti-human CD24 Antibody *HI45, XFD488 Same Structure to Alexa Fluor™ 488*

10240151 500 tests
EUR 918

XFD488 Anti-human CD25 Antibody *HI25a, XFD488 Same Structure to Alexa Fluor™ 488*

10250150 100 tests
EUR 245

XFD488 Anti-human CD25 Antibody *HI25a, XFD488 Same Structure to Alexa Fluor™ 488*

10250151 500 tests
EUR 918

XFD488 Anti-human CD25 Antibody *7G7B6, XFD488 Same Structure to Alexa Fluor™ 488*

10251150 100 tests
EUR 245

XFD488 Anti-human CD25 Antibody *7G7B6, XFD488 Same Structure to Alexa Fluor™ 488*

10251151 500 tests
EUR 918

XFD488 Anti-human CD27 Antibody *LT27, XFD488 Same Structure to Alexa Fluor™ 488*

10270150 100 tests
EUR 547

XFD488 Anti-human CD27 Antibody *LT27, XFD488 Same Structure to Alexa Fluor™ 488*

10270151 500 tests
EUR 2051

XFD488 Anti-human CD27 Antibody *O323, XFD488 Same Structure to Alexa Fluor™ 488*

10271140 100 tests
EUR 245

XFD488 Anti-human CD27 Antibody *O323, XFD488 Same Structure to Alexa Fluor™ 488*

10271141 500 tests
EUR 918

XFD488 Anti-human CD28 Antibody *CD28.2, XFD488 Same Structure to Alexa Fluor™ 488*

10280150 100 tests
EUR 245

XFD488 Anti-human CD28 Antibody *CD28.2, XFD488 Same Structure to Alexa Fluor™ 488*

10280151 500 tests
EUR 918

XFD488 Anti-human CD28 Antibody *9.3, XFD488 Same Structure to Alexa Fluor™ 488*

10281150 100 tests
EUR 245

XFD488 Anti-human CD28 Antibody *9.3, XFD488 Same Structure to Alexa Fluor™ 488*

10281151 500 tests
EUR 918

XFD488 Anti-human CD29 Antibody *HI29a, XFD488 Same Structure to Alexa Fluor™ 488*

10290150 100 tests
EUR 245

XFD488 Anti-human CD29 Antibody *HI29a, XFD488 Same Structure to Alexa Fluor™ 488*

10290151 500 tests
EUR 918

XFD488 Anti-human CD32 Antibody *3D3, XFD488 Same Structure to Alexa Fluor™ 488*

10321150 100 tests
EUR 547

XFD488 Anti-human CD32 Antibody *3D3, XFD488 Same Structure to Alexa Fluor™ 488*

10321151 500 tests
EUR 2051

XFD488 Anti-human CD34 Antibody *4H11, XFD488 Same Structure to Alexa Fluor™ 488*

10340150 100 tests
EUR 245

XFD488 Anti-human CD34 Antibody *4H11, XFD488 Same Structure to Alexa Fluor™ 488*

10340151 500 tests
EUR 918

XFD488 Anti-human CD36 Antibody *CB38, XFD488 Same Structure to Alexa Fluor™ 488*

10360150 100 tests
EUR 547

XFD488 Anti-human CD36 Antibody *CB38, XFD488 Same Structure to Alexa Fluor™ 488*

10360151 500 tests
EUR 2051

XFD488 Anti-human CD38 Antibody *HIT2, XFD488 Same Structure to Alexa Fluor™ 488*

10380150 100 tests
EUR 245

XFD488 Anti-human CD38 Antibody *HIT2, XFD488 Same Structure to Alexa Fluor™ 488*

10380151 500 tests
EUR 918

XFD488 Anti-human CD38 Antibody *HI157, XFD488 Same Structure to Alexa Fluor™ 488*

10381150 100 tests
EUR 245

XFD488 Anti-human CD38 Antibody *HI157, XFD488 Same Structure to Alexa Fluor™ 488*

10381151 500 tests
EUR 918

XFD488 Anti-human CD38 Antibody *HB7, XFD488 Same Structure to Alexa Fluor™ 488*

10382140 100 tests
EUR 245

XFD488 Anti-human CD38 Antibody *HB7, XFD488 Same Structure to Alexa Fluor™ 488*

10382141 500 tests
EUR 918

XFD488 Anti-human CD39 Antibody *TU66, XFD488 Same Structure to Alexa Fluor™ 488*

10390150 100 tests
EUR 547

XFD488 Anti-human CD39 Antibody *TU66, XFD488 Same Structure to Alexa Fluor™ 488*

10390151 500 tests
EUR 2051

XFD488 Anti-human CD40 Antibody *HI40a, XFD488 Same Structure to Alexa Fluor™ 488*

10400150 100 tests
EUR 245

XFD488 Anti-human CD40 Antibody *HI40a, XFD488 Same Structure to Alexa Fluor™ 488*

10400151 500 tests
EUR 918

XFD488 Anti-human CD40 Antibody *G28.5, XFD488 Same Structure to Alexa Fluor™ 488*

10401150 100 tests
EUR 245

XFD488 Anti-human CD40 Antibody *G28.5, XFD488 Same Structure to Alexa Fluor™ 488*

10401151 500 tests
EUR 918

XFD488 Anti-human CD41 Antibody *HIP8, XFD488 Same Structure to Alexa Fluor™ 488*

10410150 100 tests
EUR 245

XFD488 Anti-human CD41 Antibody *HIP8, XFD488 Same Structure to Alexa Fluor™ 488*

10410151 500 tests
EUR 918

XFD488 Anti-human CD41 Antibody *HIP2, XFD488 Same Structure to Alexa Fluor™ 488*

10411150 100 tests
EUR 245

XFD488 Anti-human CD41 Antibody *HIP2, XFD488 Same Structure to Alexa Fluor™ 488*

10411151 500 tests
EUR 918

XFD488 Anti-human CD42b Antibody *HIP1, XFD488 Same Structure to Alexa Fluor™ 488*

10420150 100 tests
EUR 245

XFD488 Anti-human CD42b Antibody *HIP1, XFD488 Same Structure to Alexa Fluor™ 488*

10420151 500 tests
EUR 918

XFD488 Anti-human CD43 Antibody *HI165, XFD488 Same Structure to Alexa Fluor™ 488*

10430150 100 tests
EUR 245

XFD488 Anti-human CD43 Antibody *HI165, XFD488 Same Structure to Alexa Fluor™ 488*

10430151 500 tests
EUR 918

XFD488 Anti-human CD43 Antibody *HI161, XFD488 Same Structure to Alexa Fluor™ 488*

10431150 100 tests
EUR 245

Our outcomes confirmed that ZAIS@PEI might mediate plasmid DNA supply into HeLa cells with the excessive transfection effectivity of 40% and low cytotoxicity, in the meantime permitting the real-time monitoring of gene transfection. The obtained ZAIS@PEI QDs had been verified as versatile self-tracking gene vectors, which built-in gene supply with bioimaging capabilities with out exterior labeling.

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