Successful delivery of large-size CRISPR/Cas9 vectors in hard-to-transfect human cells using small plasmids

Successful delivery of large-size CRISPR/Cas9 vectors in hard-to-transfect human cells using small plasmids

With the rise of recent highly effective genome engineering applied sciences, equivalent to CRISPR/Cas9, cell fashions might be engineered successfully to speed up fundamental and illness analysis. Essentially the most essential step on this process is the environment friendly supply of international nucleic acids into cells by mobile transfection. For the reason that vectors encoding the elements crucial for CRISPR/Cas genome engineering are at all times massive (9-19 kb), they end in low transfection effectivity and cell viability, and thus subsequent choice or purification of optimistic cells is required.

To beat these obstacles, we right here present a non-toxic and non-viral supply methodology that will increase transfection effectivity (as much as 40-fold) and cell viability (as much as 6-fold) in plenty of hard-to-transfect human most cancers cell strains and first blood cells. At its core, the approach is predicated on including exogenous small plasmids of an outlined measurement to the transfection combination. The “entire genome” TMV-based expression system, Geneware®, was used within the cGMP manufacturing of the plant-made pharmaceutical Q-Griffithsin and demonstrates steady expression for as much as a two-year interval.

Virion and plasmid banks which contained viral cDNA and a Q-Griffithsin sequence had been capable of produce >200 g of Q-Griffithsin. Knowledge assessing the standard and stability of the product banks had been measured by means of useful assessments of visible symptomology and product expression.

The cell-penetrating YopM protein-functionalized quantum dot-plasmid DNA conjugate as a novel gene supply vector

Non-viral gene supply techniques have nice potential for secure and environment friendly gene remedy, whereas inefficient mobile and nuclear uptake stay as the foremost hurdles. Novel approaches are wanted to reinforce the transfection effectivity of non-viral vectors. In accordance with this want, the target of this examine was to assemble a non-viral vector that might obtain gene supply with out utilizing further lipid-based transfection agent. We aimed to impart self-delivery property to a non-viral vector through the use of the cell and nucleus penetrating properties of YopM proteins from the three Yersinia spp. (Y. pestis, Y. enterocolotica and Y. pseudotuberculosis).

Plasmid DNA (pDNA) encoding inexperienced fluorescent protein (GFP) was labeled with quantum dots (QDs) through peptide-nucleic acid (PNA) recognition web site. Recombinant YopM protein was then hooked up to the conjugate through a second PNA recognition web site. The YopM ̶ QDs ̶ pDNA conjugate was transfected into HeLa cells with out utilizing further transfection reagent. All three conjugates produced GFP fluorescence, indicating that the plasmid was efficiently delivered to the nucleus.

As management, bare pDNA was transfected into the cells through the use of a industrial transfection reagent. The Y. pseudotuberculosis YopM-functionalized conjugate achieved the best GFP expression, in comparison with different two YopM proteins and the transfection reagent. To one of the best of our data, YopM protein was used for the primary time in a non-viral gene supply vector.

[Maintenance of Plasmid Expression in vivo Depends Primarily on the CpG Contents of the Vector and Transgene]

Plasmid-mediated gene remedy, being a secure and comparatively cheap therapeutic technique, is affected by a quick silencing of transgene expression. The silencing severely reduces the long-term effectivity of plasmid vectors. Now we have earlier constructed a low-CpG pMBR2 plasmid vector supporting extended expression of transgenes in mesenchymal stem cells in vitro. Lengthy-term expression from the pMBR2 vector was studied for the wild-type mouse secreted alkaline phosphatase gene (mSEAPTwt) and its model devoid of CpGs (mSEAP0) after vector electroporation into mouse hindlimb muscle mass and hydrodynamic supply to the liver.

Successful delivery of large-size CRISPR/Cas9 vectors in hard-to-transfect human cells using small plasmids

The mSEAP ranges within the blood had been measured over one yr. With the pMBR2-mSEAP0 assemble, the mSEAP ranges in leg muscle mass elevated greater than 2.5-fold within the first two months and remained increased than the preliminary stage till the top of the experiment. Far decrease expression ranges had been noticed with the management pCDNA3.1-mSEAP0 assemble. Expression from pMBR2-mSEAPwt decreased to about 40% after 6 months and remained at comparable ranges thereafter. Within the mouse liver, expression from pMBR2-mSEAP0 was roughly halved throughout the first 18 weeks after which lower slowly to the ultimate 17% stage.

Expression from pMBR2-mSEAPwt initially dropped to 18% and remained at roughly 10% thereafter. In distinction, expression from pCDNA3.1-mSEAP0 sharply dropped to five% after 2 weeks and remained at almost zero ranges all through the remainder of the experiment. Thus, each vector and transgene ought to have considerably diminished CpG contents to make sure extended plasmid-mediated expression within the liver, whereas minimizing the vector CpG content material is adequate for expression in skeletal muscle mass. The outcomes prompt moreover that the localization of S/MAR components throughout the transcription unit, in distinction to their outdoors location, ends in important discount of the extent of secreted, however not cytoplasmic, proteins.

Synthesis of aqueous AgInS/ZnS@PEI as a self-indicating nonviral vector for plasmid DNA self-tracking supply.

Varied nanoparticles have been employed for gene supply. Revealing the endocytosis or phagocytosis behaviors of the gene supply system is essential for designing refined gene therapies. Herein, a type of water-soluble PEI capped AgInS/ZnS quantum dot was synthesized as a self-indicating gene vector within the presence of branched polyethylenimine (PEI) and mercaptopropionic acid (MPA). The as-prepared ZAIS@PEI QDs might effectively condense plasmid DNA into nanocomplexes with robust fluorescence, which may reveal their positions throughout gene transfection.

Anti-LAMP3 antibody (Alexa-fluor 488)

STJ170004 100 µg
EUR 393
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-IL3RA antibody (Alexa-fluor 488)

STJ170009 100 µg
EUR 393
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-CD207 antibody (Alexa-fluor 488)

STJ170014 100 µg
EUR 393
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-IL7R antibody (Alexa-fluor 488)

STJ170020 100 µg
EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Anti-Hu CD16 Alexa Fluor® 488

A4-646-T100 100 tests
EUR 269

Goat anti Mouse IgG1 (Alexa Fluor 488)

43R-1649 500 ug
EUR 570
Description: Goat anti Mouse IgG1 secondary antibody (Alexa Fluor 488)

Anti-Hu CD72 Alexa Fluor® 488

A4-310-T100 100 tests
EUR 269

Anti-Bov CD9 Alexa Fluor® 488

A4-354-C100 0.1 mg
EUR 269

AF488-streptavidin conjugate [Streptavidin, Alexa Fluor™ 488 Conjugate]

16891 1 mg
EUR 176
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

AF488 Phalloidin [equivalent to Alexa Fluor® 488 phalloidin]

23153 300 Tests
EUR 306
  • R-phrase: R23, R24, R25
  • H-Phrase: H301, H311, H331
  • Symbol for dangerous compounds: T
  • UNSPEC Code: 12352200

Endoglin/CD105 Alexa Fluor

FC15024 100 Tests
EUR 448

Goat Anti-Mouse IgG(H+L) Alexa Fluor 488–conjugated

S0017 200ul
EUR 304

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 488–conjugated

S0018 200ul
EUR 304

Anti-Hu CD3 zeta (pY153) Alexa Fluor® 488

A4-686-C100 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY72) Alexa Fluor® 488

A4-712-C100 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY142) Alexa Fluor® 488

A4-730-C100 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY111) Alexa Fluor® 488

A4-737-C100 0.1 mg
EUR 269

Donkey Anti-Rabbit IgG (H+L), Alexa Fluor® 488 Conjugated

Ab8032-001 0.5mg
EUR 435

SAM FCM (Alexa Fluor 647)

abx098902-100tests 100 tests
EUR 1233
  • Shipped within 5-10 working days.

Anti-LAMP3 (human) Monoclonal Antibody (104G4) (Alexa Fluor® 488)

M09406 100ug
EUR 565
Description: Mouse Monoclonal LAMP3 (human) Antibody (104G4) (Alexa Fluor® 488). Validated in IHC and tested in Human.

Alpha Fluor™ 488 amine

1705 1 mg
EUR 306
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Alpha Fluor™ 488 Hydroxylamine

1900 1 mg
EUR 306
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Anti-LAMP3 antibody (Alexa-fluor 546)

STJ170005 100 µg
EUR 393
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-LAMP3 antibody (Alexa-fluor 647)

STJ170006 100 µg
EUR 393
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-IL3RA antibody (Alexa-fluor 546)

STJ170010 100 µg
EUR 393
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-IL3RA antibody (Alexa-fluor 647)

STJ170011 100 µg
EUR 393
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-CD207 antibody (Alexa-fluor 546)

STJ170015 100 µg
EUR 393
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-CD207 antibody (Alexa-fluor 647)

STJ170016 100 µg
EUR 393
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-IL7R antibody (Alexa-fluor 546)

STJ170021 100 µg
EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Anti-IL7R antibody (Alexa-fluor 647)

STJ170022 100 µg
EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Anti-Langerin (human) Monoclonal Antibody (DCGM4/122D5) (Alexa Fluor® 488)

M02316 100ug
EUR 580
Description: Mouse Monoclonal Langerin (human) Antibody (DCGM4/122D5) (Alexa Fluor® 488). Validated in IHC and tested in Human.

Alpha Fluor™ 488 NHS Ester

1812 1 mg
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Streptavidin-Alexa488 (Alexas fluor 488) conjugate

SV-A488-100 100 tests
EUR 225

Rabbit Anti-Rat IgG (H+L)-Alexa 488 Fluor conjugate (adsorbed with human IgG)

50336 0.5 ml
EUR 225

Alpha Fluor™ 532 acid [equivalent to Alexa Fluor™ 532 acid]

1795 10 mg
EUR 393
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Mouse IgG1-Alexa 488 conjugate (isotype control)

20102-101-A488 50 Tests
EUR 263

Anti-Hu CD30 Alexa Fluor® 700

A7-455-T100 100 tests
EUR 269

Anti-Hu CD94 Alexa Fluor® 700

A7-727-T100 100 tests
EUR 269

Anti-Hu CD56 Alexa Fluor® 700

A7-789-T100 100 tests
EUR 269

Donkey anti Goat IgG (H + L) (Alexa Fluor 594)

43R-ID005AF 500 ug
EUR 338
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)

43R-ID022AF 500 ug
EUR 364
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Goat IgG (H + L) (Alexa Fluor 647)

43R-ID028AF 500 ug
EUR 430
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 647)

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)

43R-ID047AF 500 ug
EUR 462
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Chicken IgY (H + L) (Alexa Fluor 594)

43R-ID056AF 500 ug
EUR 343
Description: Donkey anti Chicken IgY secondary antibody (H + L) (Alexa Fluor 594)

Donkey anti Chicken IgY (H + L) (Alexa Fluor 647)

43R-ID060AF 300 ug
EUR 425
Description: Donkey anti Chicken IgY (H + L) (Fab'2) (Alexa Fluor 647)

Rabbit anti Chicken IgY (H + L) (Alexa Fluor 594)

43R-IR016AF 1 mg
EUR 281
Description: Rabbit anti Chicken IgY (H + L) secondary antibody (Alexa Fluor 594)

AF350-streptavidin conjugate [Streptavidin, Alexa Fluor™ 350 Conjugate]

16890 1 mg
EUR 176
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

AF594-streptavidin conjugate [Streptavidin, Alexa Fluor™ 594 Conjugate]

16892 1 mg
EUR 176
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

AF350 Phalloidin [equivalent to Alexa Fluor® 350 phalloidin]

23150 300 Tests
EUR 306
  • R-phrase: R23, R24, R25
  • H-Phrase: H301, H311, H331
  • Symbol for dangerous compounds: T
  • UNSPEC Code: 12352200

AF594 Phalloidin [equivalent to Alexa Fluor® 594 phalloidin]

23158 300 Tests
EUR 306
  • R-phrase: R23, R24, R25
  • H-Phrase: H301, H311, H331
  • Symbol for dangerous compounds: T
  • UNSPEC Code: 12352200

Goat Anti-Mouse IgG(H+L) Alexa Fluor 594–conjugated

S0005 200ul
EUR 376

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 594–conjugated

S0006 200ul
EUR 376

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 647–conjugated

S0013 200ul
EUR 304

Goat Anti-Mouse IgG(H+L) Alexa Fluor 647–conjugated

S0014 200ul
EUR 304

Mouse pre-microRNA Expression Construct mir-488

MMIR-488-PA-1 Bacterial Streak
EUR 684
  • Category: MicroRNA Tools

Anti-Hu CD3 zeta (pY153) Alexa Fluor® 647

A6-686-C100 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY72) Alexa Fluor® 647

A6-712-C100 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY142) Alexa Fluor® 647

A6-730-C100 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY111) Alexa Fluor® 647

A6-737-C100 0.1 mg
EUR 269

Donkey Anti-Goat IgG (H+L), Alexa Fluor® 594 Conjugated

Ab8011-001 1mg
EUR 334

Donkey anti Goat IgG (H + L) (Fab 2) (Alexa Fluor 594)

43R-ID012AF 300 ug
EUR 410
Description: Donkey anti Goat IgG (H + L) secondary antibody (Fab'2) (Alexa Fluor 594)

Rabbit Anti-Rat IgG (H+L)-Alexa 594 Fluor conjugate (adsorbed with human IgG)

50337 0.5 ml
EUR 225

Recombinant (E.Coli) Hepatitis C Virus (HCV) NS5 Genotype-2a 

RP-488 100 ug
EUR 286

Tide Fluor 2-LL-37

H-8286.0100 0.1mg
EUR 312
Description: Sum Formula: C205H340N60O53+dye

Tide Fluor 2-LL-37

H-8286.0500 0.5mg
EUR 1017
Description: Sum Formula: C205H340N60O53+dye

Anti-Cytokeratins Alexa Fluor488

A4-108-C025 0.025 mg
EUR 175

Anti-Cytokeratins Alexa Fluor488

A4-108-C100 0.1 mg
EUR 310

Anti-PSMA Alexa Fluor488

A4-539-C025 0.025 mg
EUR 227

Anti-PSMA Alexa Fluor488

A4-539-C100 0.1 mg
EUR 414

Anti-FoxP3 Alexa Fluor488

A4-601-C025 0.025 mg
EUR 201

Anti-FoxP3 Alexa Fluor488

A4-601-C100 0.1 mg
EUR 362

Anti-Phosphotyrosine Alexa Fluor647

A6-263-C025 0.025 mg
EUR 154

Anti-Phosphotyrosine Alexa Fluor647

A6-263-C100 0.1 mg
EUR 269

Anti-LCK Alexa Fluor647

A6-269-C025 0.025 mg
EUR 206

Anti-LCK Alexa Fluor647

A6-269-C100 0.1 mg
EUR 373

Anti-FoxP3 Alexa Fluor647

A6-601-C025 0.025 mg
EUR 201

Anti-FoxP3 Alexa Fluor647

A6-601-C100 0.1 mg
EUR 362

Monoclonal Anti-Monkey IgG-Alexa 488 Conj. (specific for monkey; no reactivity with human or animals IgG)

70030-AF488 50 tests
EUR 347

CellBriteâ„¢ Fix 488

30090 1KIT
EUR 644
Description: Minimum order quantity: 1 unit of 1KIT

Tide Fluor 2-LL-37 (scrambled)

H-8288.0100 0.1mg
EUR 312
Description: Sum Formula: C205H340N60O53+dye

Tide Fluor 2-LL-37 (scrambled)

H-8288.0500 0.5mg
EUR 1017
Description: Sum Formula: C205H340N60O53+dye

Tide Fluor 5WS-o-Conotoxin GVIA

H-8356.0100 0.1mg
EUR 1146
Description: Sum Formula: C120H182N38O43S6+dye

Alpha Fluor™ 532 NHS Ester

1819 1 mg
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Alpha Fluor™ 594 C5 Maleimide

1891 1 mg
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Metal Fluor™ Zn-520, AM

21263 1 mg
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Helix Fluor™ 6-JOE Phosphoramidite

6046 100 umoles
EUR 50
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Streptavidin-Alexa594 (Alexas fluor 594) conjugate

SV-A594-100 100 tests
EUR 225

Anti-Cytokeratin 18 Alexa Fluor488

A4-106-C025 0.025 mg
EUR 186

Anti-Cytokeratin 18 Alexa Fluor488

A4-106-C100 0.1 mg
EUR 331

Anti-Cytokeratin 19 Alexa Fluor488

A4-120-C025 0.025 mg
EUR 186

Anti-Cytokeratin 19 Alexa Fluor488

A4-120-C100 0.1 mg
EUR 331

Anti-Ki-67 Alexa Fluor488

A4-155-T025 25 tests
EUR 154

Anti-Ki-67 Alexa Fluor488

A4-155-T100 100 tests
EUR 269

Anti-Hu CD45 Alexa Fluor488

A4-160-T100 100 tests
EUR 269

Anti-Hu CD193 Alexa Fluor488

A4-161-T100 100 tests
EUR 269

Anti-Hu CD279 Alexa Fluor488

A4-176-T100 100 tests
EUR 269

Anti-Hu CD43 Alexa Fluor488

A4-220-T025 25 tests
EUR 154

Anti-Hu CD43 Alexa Fluor488

A4-220-T100 100 tests
EUR 269

Anti-Hu CD44 Alexa Fluor488

A4-221-T025 25 tests
EUR 154

Anti-Hu CD44 Alexa Fluor488

A4-221-T100 100 tests
EUR 269

Anti-Hu CD45 Alexa Fluor488

A4-222-T025 25 tests
EUR 154

Anti-Hu CD45 Alexa Fluor488

A4-222-T100 100 tests
EUR 269

Anti-Hu CD55 Alexa Fluor488

A4-230-T025 25 tests
EUR 154

Anti-Hu CD55 Alexa Fluor488

A4-230-T100 100 tests
EUR 269

Anti-Hu CD50 Alexa Fluor488

A4-266-T025 25 tests
EUR 154

Anti-Hu CD50 Alexa Fluor488

A4-266-T100 100 tests
EUR 269

Anti-Hu CD31 Alexa Fluor488

A4-273-T025 25 tests
EUR 154

Anti-Hu CD31 Alexa Fluor488

A4-273-T100 100 tests
EUR 269

Anti-Hu CD147 Alexa Fluor488

A4-274-T025 25 tests
EUR 154

Anti-Hu CD147 Alexa Fluor488

A4-274-T100 100 tests
EUR 269

Anti-Hu CD34 Alexa Fluor488

A4-297-T025 25 tests
EUR 154

Anti-Hu CD34 Alexa Fluor488

A4-297-T100 100 tests
EUR 269

Anti-Hu CD105 Alexa Fluor488

A4-298-T025 25 tests
EUR 154

Anti-Hu CD105 Alexa Fluor488

A4-298-T100 100 tests
EUR 269

Anti-Hu CD41 Alexa Fluor488

A4-309-T025 25 tests
EUR 154

Anti-Hu CD41 Alexa Fluor488

A4-309-T100 100 tests
EUR 269

Anti-Hu CD72 Alexa Fluor488

A4-310-T025 25 tests
EUR 154

Anti-Hu CD63 Alexa Fluor488

A4-343-T025 25 tests
EUR 154

Anti-Hu CD63 Alexa Fluor488

A4-343-T100 100 tests
EUR 269

Anti-Hu CD13 Alexa Fluor488

A4-396-T025 25 tests
EUR 154

Anti-Hu CD13 Alexa Fluor488

A4-396-T100 100 tests
EUR 269

Anti-HLA-G Alexa Fluor488

A4-431-C025 0.025 mg
EUR 217

Anti-HLA-G Alexa Fluor488

A4-431-C100 0.1 mg
EUR 394

Anti-HLA-G Alexa Fluor488

A4-437-C025 0.025 mg
EUR 217

Anti-HLA-G Alexa Fluor488

A4-437-C100 0.1 mg
EUR 394

Anti-Hu CD300a Alexa Fluor488

A4-501-T100 100 tests
EUR 269

Anti-HLA-A2 Alexa Fluor488

A4-556-T025 25 tests
EUR 154

Anti-HLA-A2 Alexa Fluor488

A4-556-T100 100 tests
EUR 269

Anti-CD3 zeta Alexa Fluor488

A4-568-C100 0.1 mg
EUR 269

Anti-Ms CD8a Alexa Fluor488

A4-579-C025 0.025 mg
EUR 139

Anti-Ms CD8a Alexa Fluor488

A4-579-C100 0.1 mg
EUR 238

Anti-Hu CD326 Alexa Fluor488

A4-582-T100 100 tests
EUR 269

Anti-Hu CD3 Alexa Fluor488

A4-631-T100 100 tests
EUR 269

Anti-Hu CD16 Alexa Fluor488

A4-646-T025 25 tests
EUR 154

Anti-Hu CD150 Alexa Fluor488

A4-660-T100 100 tests
EUR 269

Anti-Hu CD107a Alexa Fluor488

A4-671-T025 25 tests
EUR 154

Anti-Hu CD107a Alexa Fluor488

A4-671-T100 100 tests
EUR 269

Anti-Hu CD107b Alexa Fluor488

A4-672-T025 25 tests
EUR 154

Anti-Hu CD107b Alexa Fluor488

A4-672-T100 100 tests
EUR 269

Anti-Hu CD73 Alexa Fluor488

A4-675-T100 100 tests
EUR 269

Anti-Hu CD11b Alexa Fluor488

A4-681-T025 25 tests
EUR 154

Anti-Hu CD11b Alexa Fluor488

A4-681-T100 100 tests
EUR 269

Anti-Hu CD35 Alexa Fluor488

A4-703-T100 100 tests
EUR 269

Anti-Hu CD305 Alexa Fluor488

A4-713-T100 100 tests
EUR 269

Anti-Hu CD161 Alexa Fluor488

A4-729-T100 100 tests
EUR 269

Anti-Hu CD144 Alexa Fluor488

A4-770-T100 100 tests
EUR 269

Anti-HLA-ABCE Alexa Fluor488

A4-813-C100 0.1 mg
EUR 373

Anti-Ki-67 Alexa Fluor647

A6-155-T025 25 tests
EUR 154

Anti-Ki-67 Alexa Fluor647

A6-155-T100 100 tests
EUR 269

Anti-Hu CD45 Alexa Fluor647

A6-160-T100 100 tests
EUR 269

Anti-Hu CD193 Alexa Fluor647

A6-161-T100 100 tests
EUR 269

Anti-Hu CD279 Alexa Fluor647

A6-176-T100 100 tests
EUR 269

Anti-Hu CD273 Alexa Fluor647

A6-178-T100 100 tests
EUR 269

Anti-Hu CD231 Alexa Fluor647

A6-200-T100 100 tests
EUR 269

Anti-Hu CD9 Alexa Fluor647

A6-208-T025 25 tests
EUR 154

Anti-Hu CD9 Alexa Fluor647

A6-208-T100 100 tests
EUR 269

Anti-Hu CD45 Alexa Fluor647

A6-222-T025 25 tests
EUR 154

Anti-Hu CD45 Alexa Fluor647

A6-222-T100 100 tests
EUR 269

Anti-Hu CD45RB Alexa Fluor647

A6-224-T025 25 tests
EUR 154

Anti-Hu CD45RB Alexa Fluor647

A6-224-T100 100 tests
EUR 269

Anti-Hu CD55 Alexa Fluor647

A6-230-T025 25 tests
EUR 154

Anti-Hu CD55 Alexa Fluor647

A6-230-T100 100 tests
EUR 269

Anti-Hu CD59 Alexa Fluor647

A6-233-T025 25 tests
EUR 154

Anti-Hu CD59 Alexa Fluor647

A6-233-T100 100 tests
EUR 269

Anti-Hu CD71 Alexa Fluor647

A6-235-T025 25 tests
EUR 154

Anti-Hu CD71 Alexa Fluor647

A6-235-T100 100 tests
EUR 269

Anti-Hu CD21 Alexa Fluor647

A6-306-T025 25 tests
EUR 154

Anti-Hu CD21 Alexa Fluor647

A6-306-T100 100 tests
EUR 269

Anti-Hu CD177 Alexa Fluor647

A6-314-T025 25 tests
EUR 154

Anti-Hu CD177 Alexa Fluor647

A6-314-T100 100 tests
EUR 269

Anti-Hu CD222 Alexa Fluor647

A6-315-T025 25 tests
EUR 154

Anti-Hu CD222 Alexa Fluor647

A6-315-T100 100 tests
EUR 269

Anti-Hu CD30 Alexa Fluor647

A6-455-T025 25 tests
EUR 154

Anti-Hu CD30 Alexa Fluor647

A6-455-T100 100 tests
EUR 269

Anti-gamma-Tubulin Alexa Fluor647

A6-465-C025 0.025 mg
EUR 217

Anti-gamma-Tubulin Alexa Fluor647

A6-465-C100 0.1 mg
EUR 394

Anti-Hu CD264 Alexa Fluor647

A6-519-C100 0.1 mg
EUR 269

Anti-Hu CD69 Alexa Fluor647

A6-552-T025 25 tests
EUR 154

Anti-Hu CD69 Alexa Fluor647

A6-552-T100 100 tests
EUR 269

Anti-HLA-A2 Alexa Fluor647

A6-556-T025 25 tests
EUR 154

Anti-HLA-A2 Alexa Fluor647

A6-556-T100 100 tests
EUR 269

Anti-Hu CD326 Alexa Fluor647

A6-581-T025 25 tests
EUR 154

Anti-Hu CD326 Alexa Fluor647

A6-581-T100 100 tests
EUR 269

Anti-Hu CD326 Alexa Fluor647

A6-582-T100 100 tests
EUR 269

Anti-Hu CD158d Alexa Fluor647

A6-609-T100 100 tests
EUR 269

Anti-Hu CD3 Alexa Fluor647

A6-631-T100 100 tests
EUR 269

Anti-Hu CD209 Alexa Fluor647

A6-640-T100 100 tests
EUR 269

Anti-Hu CD64 Alexa Fluor647

A6-644-T025 25 tests
EUR 154

Anti-Hu CD64 Alexa Fluor647

A6-644-T100 100 tests
EUR 269

Anti-Hu CD16 Alexa Fluor647

A6-646-T025 25 tests
EUR 154

Anti-Hu CD16 Alexa Fluor647

A6-646-T100 100 tests
EUR 269

Anti-Hu CD150 Alexa Fluor647

A6-660-T100 100 tests
EUR 269

Anti-Hu CD73 Alexa Fluor647

A6-675-T100 100 tests
EUR 269

Anti-HLA-DR Alexa Fluor647

A6-690-T025 25 tests
EUR 154

Anti-HLA-DR Alexa Fluor647

A6-690-T100 100 tests
EUR 269

Anti-Hu CD161 Alexa Fluor647

A6-729-T100 100 tests
EUR 269

Anti-Hu CD200 Alexa Fluor647

A6-746-T100 100 tests
EUR 269

Anti-Hu CD160 Alexa Fluor647

A6-750-T100 100 tests
EUR 269

Anti-Hu CD243 Alexa Fluor647

A6-764-T100 100 tests
EUR 269

Our outcomes confirmed that ZAIS@PEI might mediate plasmid DNA supply into HeLa cells with the excessive transfection effectivity of 40% and low cytotoxicity, in the meantime permitting the real-time monitoring of gene transfection. The obtained ZAIS@PEI QDs had been verified as versatile self-tracking gene vectors, which built-in gene supply with bioimaging capabilities with out exterior labeling.

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