With the rise of recent highly effective genome engineering applied sciences, equivalent to CRISPR/Cas9, cell fashions might be engineered successfully to speed up fundamental and illness analysis. Essentially the most essential step on this process is the environment friendly supply of international nucleic acids into cells by mobile transfection. For the reason that vectors encoding the elements crucial for CRISPR/Cas genome engineering are at all times massive (9-19 kb), they end in low transfection effectivity and cell viability, and thus subsequent choice or purification of optimistic cells is required.
To beat these obstacles, we right here present a non-toxic and non-viral supply methodology that will increase transfection effectivity (as much as 40-fold) and cell viability (as much as 6-fold) in plenty of hard-to-transfect human most cancers cell strains and first blood cells. At its core, the approach is predicated on including exogenous small plasmids of an outlined measurement to the transfection combination. The “entire genome” TMV-based expression system, Geneware®, was used within the cGMP manufacturing of the plant-made pharmaceutical Q-Griffithsin and demonstrates steady expression for as much as a two-year interval.
Virion and plasmid banks which contained viral cDNA and a Q-Griffithsin sequence had been capable of produce >200 g of Q-Griffithsin. Knowledge assessing the standard and stability of the product banks had been measured by means of useful assessments of visible symptomology and product expression.
The cell-penetrating YopM protein-functionalized quantum dot-plasmid DNA conjugate as a novel gene supply vector
Non-viral gene supply techniques have nice potential for secure and environment friendly gene remedy, whereas inefficient mobile and nuclear uptake stay as the foremost hurdles. Novel approaches are wanted to reinforce the transfection effectivity of non-viral vectors. In accordance with this want, the target of this examine was to assemble a non-viral vector that might obtain gene supply with out utilizing further lipid-based transfection agent. We aimed to impart self-delivery property to a non-viral vector through the use of the cell and nucleus penetrating properties of YopM proteins from the three Yersinia spp. (Y. pestis, Y. enterocolotica and Y. pseudotuberculosis).
Plasmid DNA (pDNA) encoding inexperienced fluorescent protein (GFP) was labeled with quantum dots (QDs) through peptide-nucleic acid (PNA) recognition web site. Recombinant YopM protein was then hooked up to the conjugate through a second PNA recognition web site. The YopM ̶ QDs ̶ pDNA conjugate was transfected into HeLa cells with out utilizing further transfection reagent. All three conjugates produced GFP fluorescence, indicating that the plasmid was efficiently delivered to the nucleus.
As management, bare pDNA was transfected into the cells through the use of a industrial transfection reagent. The Y. pseudotuberculosis YopM-functionalized conjugate achieved the best GFP expression, in comparison with different two YopM proteins and the transfection reagent. To one of the best of our data, YopM protein was used for the primary time in a non-viral gene supply vector.
[Maintenance of Plasmid Expression in vivo Depends Primarily on the CpG Contents of the Vector and Transgene]
Plasmid-mediated gene remedy, being a secure and comparatively cheap therapeutic technique, is affected by a quick silencing of transgene expression. The silencing severely reduces the long-term effectivity of plasmid vectors. Now we have earlier constructed a low-CpG pMBR2 plasmid vector supporting extended expression of transgenes in mesenchymal stem cells in vitro. Lengthy-term expression from the pMBR2 vector was studied for the wild-type mouse secreted alkaline phosphatase gene (mSEAPTwt) and its model devoid of CpGs (mSEAP0) after vector electroporation into mouse hindlimb muscle mass and hydrodynamic supply to the liver.
The mSEAP ranges within the blood had been measured over one yr. With the pMBR2-mSEAP0 assemble, the mSEAP ranges in leg muscle mass elevated greater than 2.5-fold within the first two months and remained increased than the preliminary stage till the top of the experiment. Far decrease expression ranges had been noticed with the management pCDNA3.1-mSEAP0 assemble. Expression from pMBR2-mSEAPwt decreased to about 40% after 6 months and remained at comparable ranges thereafter. Within the mouse liver, expression from pMBR2-mSEAP0 was roughly halved throughout the first 18 weeks after which lower slowly to the ultimate 17% stage.
Expression from pMBR2-mSEAPwt initially dropped to 18% and remained at roughly 10% thereafter. In distinction, expression from pCDNA3.1-mSEAP0 sharply dropped to five% after 2 weeks and remained at almost zero ranges all through the remainder of the experiment. Thus, each vector and transgene ought to have considerably diminished CpG contents to make sure extended plasmid-mediated expression within the liver, whereas minimizing the vector CpG content material is adequate for expression in skeletal muscle mass. The outcomes prompt moreover that the localization of S/MAR components throughout the transcription unit, in distinction to their outdoors location, ends in important discount of the extent of secreted, however not cytoplasmic, proteins.
Synthesis of aqueous AgInS/ZnS@PEI as a self-indicating nonviral vector for plasmid DNA self-tracking supply.
Varied nanoparticles have been employed for gene supply. Revealing the endocytosis or phagocytosis behaviors of the gene supply system is essential for designing refined gene therapies. Herein, a type of water-soluble PEI capped AgInS/ZnS quantum dot was synthesized as a self-indicating gene vector within the presence of branched polyethylenimine (PEI) and mercaptopropionic acid (MPA). The as-prepared ZAIS@PEI QDs might effectively condense plasmid DNA into nanocomplexes with robust fluorescence, which may reveal their positions throughout gene transfection.
CD3 Mouse mAb (Alexa Fluor 488) |
|||
| MBS4753570-01mL | MyBiosource | 0.1mL | EUR 450 |
CD3 Mouse mAb (Alexa Fluor 488) |
|||
| MBS4753570-5x01mL | MyBiosource | 5x0.1mL | EUR 1540 |
Alexa Fluor® 488 anti-GFP |
|||
| GT11408 | Sony | 100 Tests | Ask for price |
Alexa Fluor® 488 anti-Bax |
|||
| GT18954 | Sony | 100 µg | Ask for price |
Alexa Fluor® 488 anti-GFP |
|||
| GT11407 | Sony | 25 Tests | Ask for price |
Alexa Fluor® 488 anti-Bax |
|||
| GT18953 | Sony | 25 µg | Ask for price |
Anti-Hu CD16 Alexa Fluor 488 |
|||
| MBS1801989-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Alexa Fluor® 488 anti-BrdU |
|||
| GT14586 | Sony | 100 Tests | Ask for price |
Alexa Fluor® 488 anti-IRF7 |
|||
| GT19226 | Sony | 25 Tests | Ask for price |
Alexa Fluor® 488 anti-IRF7 |
|||
| GT19227 | Sony | 100 Tests | Ask for price |
Alexa Fluor® 488 anti-IRF4 |
|||
| GT19087 | Sony | 25 µg | Ask for price |
Alexa Fluor® 488 anti-IRF4 |
|||
| GT19088 | Sony | 100 µg | Ask for price |
Alexa Fluor® 488 anti-SOX2 |
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| GT19235 | Sony | 25 µg | Ask for price |
Alexa Fluor® 488 anti-SOX2 |
|||
| GT19236 | Sony | 100 µg | Ask for price |
Alexa Fluor® 488 anti-BrdU |
|||
| GT14585 | Sony | 25 Tests | Ask for price |
Mouse Human CD3 (Alexa Fluor 488) |
|||
| MBS8559316-01mL | MyBiosource | 0.1mL | EUR 305 |
Mouse Human CD3 (Alexa Fluor 488) |
|||
| MBS8559316-01mLAF405L | MyBiosource | 0.1mL(AF405L) | EUR 565 |
Mouse Human CD3 (Alexa Fluor 488) |
|||
| MBS8559316-01mLAF405S | MyBiosource | 0.1mL(AF405S) | EUR 565 |
Mouse Human CD3 (Alexa Fluor 488) |
|||
| MBS8559316-01mLAF610 | MyBiosource | 0.1mL(AF610) | EUR 565 |
Mouse Human CD3 (Alexa Fluor 488) |
|||
| MBS8559316-01mLAF635 | MyBiosource | 0.1mL(AF635) | EUR 565 |
Alexa Fluor® 488 anti-Hsp70 |
|||
| GT19108 | Sony | 100 Tests | Ask for price |
Alexa Fluor® 488 anti-Hsp70 |
|||
| GT19107 | Sony | 25 Tests | Ask for price |
Alexa Fluor® 488 anti-GATA4 |
|||
| GT19717 | Sony | 25 Tests | Ask for price |
Alexa Fluor® 488 anti-GATA4 |
|||
| GT19718 | Sony | 100 Tests | Ask for price |
Alexa Fluor® 488 anti-GATA3 |
|||
| GT19199 | Sony | 25 Tests | Ask for price |
Alexa Fluor® 488 anti-GATA3 |
|||
| GT19200 | Sony | 100 Tests | Ask for price |
Alexa Fluor 488 anti-human CD3 |
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| MBS9459431-25Tests | MyBiosource | 25Tests | EUR 230 |
Alexa Fluor 488 anti-human CD3 |
|||
| MBS9459431-50Tests | MyBiosource | 50Tests | EUR 290 |
Alexa Fluor 488 anti-human CD3 |
|||
| MBS9459431-5x50Tests | MyBiosource | 5x50Tests | EUR 1160 |
Alexa Fluor 488 anti-human CD3 |
|||
| MBS9459432-25Tests | MyBiosource | 25Tests | EUR 230 |
Alexa Fluor 488 anti-human CD3 |
|||
| MBS9459432-50Tests | MyBiosource | 50Tests | EUR 290 |
Alexa Fluor 488 anti-human CD3 |
|||
| MBS9459432-5x50Tests | MyBiosource | 5x50Tests | EUR 1160 |
Alexa Fluor® 488 anti-P2RY12 |
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| GT19535 | Sony | 25 µg | Ask for price |
Alexa Fluor® 488 anti-NFATc1 |
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| GT19111 | Sony | 25 µg | Ask for price |
Alexa Fluor® 488 anti-P2RY12 |
|||
| GT19536 | Sony | 100 µg | Ask for price |
Alexa Fluor® 488 anti-NFATc1 |
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| GT19112 | Sony | 100 µg | Ask for price |
Alexa Fluor® 488 anti-Vimentin |
|||
| GT19460 | Sony | 100 µg | Ask for price |
Alexa Fluor® 488 anti-Puromycin |
|||
| GT15842 | Sony | 25 Tests | Ask for price |
Alexa Fluor® 488 anti-Puromycin |
|||
| GT15843 | Sony | 100 Tests | Ask for price |
Alexa Fluor® 488 Reference Standard |
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| BLI886A-1 | Polysciences Europe GmbH | 1ml | EUR 303.48 |
Alexa Fluor® 488 Reference Standard |
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| BLI886B-5 | Polysciences Europe GmbH | 5ml | EUR 598.32 |
Alexa Fluor® 488 Reference Standard |
|||
| BLI886C-14 | Polysciences Europe GmbH | 2x7ml | EUR 1153.44 |
Alexa Fluor® 488 anti-Phosphotyrosine |
|||
| GT08826 | Sony | 100 µg | Ask for price |
Alexa Fluor® 488 anti-rat CD3 |
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| E16FRG003-100U | EnoGene | 100 μg | EUR 845 |
|
Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-rat CD4 |
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| E16FRG004-025U | EnoGene | 25 μg | EUR 368.33 |
|
Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-rat CD3 |
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| GT06363 | Sony | 100 µg | Ask for price |
Alexa Fluor® 488 anti-c-Fos |
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| GT18716 | Sony | 100 Tests | Ask for price |
Alexa Fluor® 488 anti-rat CD4 |
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| GT06379 | Sony | 25 µg | Ask for price |
Alexa Fluor® 488 anti-Bcl-2 |
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| GT18945 | Sony | 25 µg | Ask for price |
Alexa Fluor® 488 anti-T-bet |
|||
| GT19063 | Sony | 25 µg | Ask for price |
Alexa Fluor® 488 anti-T-bet |
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| GT19064 | Sony | 100 µg | Ask for price |
Alexa Fluor® 488 anti-Bcl-2 |
|||
| GT19269 | Sony | 25 Tests | Ask for price |
Alexa Fluor® 488 anti-Bcl-2 |
|||
| GT19270 | Sony | 100 Tests | Ask for price |
Alexa Fluor® 488 anti-c-Fos |
|||
| GT18715 | Sony | 25 Tests | Ask for price |
Alexa Fluor® 488 anti-Bcl-2 |
|||
| GT18946 | Sony | 100 µg | Ask for price |
Alexa Fluor® 488 anti-Pax-5 |
|||
| GT19117 | Sony | 25 µg | Ask for price |
Alexa Fluor® 488 anti-Pax-5 |
|||
| GT19118 | Sony | 100 µg | Ask for price |
Quantum™ Alexa Fluor® 488 MESF |
|||
| BLI488A-1 | Polysciences Europe GmbH | 1ml | EUR 645.84 |
Quantum™ Alexa Fluor® 488 MESF |
|||
| BLI488B-5 | Polysciences Europe GmbH | 5ml | EUR 1249.56 |
Quantum™ Alexa Fluor® 488 MESF |
|||
| BLI488C-14 | Polysciences Europe GmbH | 2x7ml | EUR 2026.08 |
Alexa Fluor® 488 anti-rat CD25 |
|||
| E16FRG025-100U | EnoGene | 100 μg | EUR 845 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-rat CD45 |
|||
| E16FRG045-100U | EnoGene | 100 μg | EUR 845 |
|
Description: Available in various conjugation types. |
|||
Mouse Human CD3 (Alexa Fluor® 488) |
|||
| E2790292 | EnoGene | 100ul | EUR 225 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-rat CD25 |
|||
| GT06410 | Sony | 100 µg | Ask for price |
Alexa Fluor® 488 anti-rat CD45 |
|||
| GT06419 | Sony | 100 µg | Ask for price |
Alexa Fluor® 488 anti-ZAP-70 |
|||
| GT19412 | Sony | 25 Tests | Ask for price |
Alexa Fluor® 488 anti-ZAP-70 |
|||
| GT19413 | Sony | 100 Tests | Ask for price |
Alexa Fluor® 488 anti-mouse CD3 |
|||
| E16FMG003-025U | EnoGene | 25 μg | EUR 368.33 |
|
Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-mouse CD3 |
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| E16FMG003-100U | EnoGene | 100 μg | EUR 845 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse CD4 |
|||
| E16FMG004-025U | EnoGene | 25 μg | EUR 368.33 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse CD5 |
|||
| E16FMG005-100U | EnoGene | 100 μg | EUR 845 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse IgD |
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| E16SDG501-025U | EnoGene | 25 μg | EUR 411.67 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse IgD |
|||
| E16SDG501-100U | EnoGene | 100 μg | EUR 975 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse IgE |
|||
| E16SEG501-025U | EnoGene | 25 μg | EUR 325 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse IgE |
|||
| E16SEG501-100U | EnoGene | 100 μg | EUR 845 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse IgM |
|||
| E16SMG501-025U | EnoGene | 25 μg | EUR 325 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse IgM |
|||
| E16SMG501-100U | EnoGene | 100 μg | EUR 845 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-human CD3 |
|||
| E16FHG003-025 | EnoGene | 25 tests | EUR 288.33 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-human CD3 |
|||
| E16FHG003-050 | EnoGene | 50 tests | EUR 411.67 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-human CD3 |
|||
| E16FHG003-100 | EnoGene | 100 Tests | EUR 682.67 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-human CD4 |
|||
| E16FHG004-050 | EnoGene | 25 tests | EUR 343 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-human CD4 |
|||
| E16FHG004-100 | EnoGene | 100 tests | EUR 457.33 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-human CD8 |
|||
| E16FHG008-025 | EnoGene | 25 tests | EUR 288.33 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-human CD8 |
|||
| E16FHG008-050 | EnoGene | 50 tests | EUR 411.67 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-human CD8 |
|||
| E16FHG008-100 | EnoGene | 100 tests | EUR 682.67 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse CD4 |
|||
| GT00195 | Sony | 100 µg | Ask for price |
Alexa Fluor® 488 anti-mouse CD4 |
|||
| GT00198 | Sony | 25 µg | Ask for price |
Alexa Fluor® 488 anti-OCT4 (OCT3) |
|||
| GT19193 | Sony | 25 Tests | Ask for price |
Alexa Fluor® 488 anti-mouse CD4 |
|||
| GT00130 | Sony | 100 µg | Ask for price |
Alexa Fluor® 488 anti-mouse CD4 |
|||
| GT00132 | Sony | 25 µg | Ask for price |
Alexa Fluor® 488 anti-human CD3 |
|||
| GT06694 | Sony | 25 Tests | Ask for price |
Alexa Fluor® 488 anti-human CD4 |
|||
| GT06793 | Sony | 100 Tests | Ask for price |
Alexa Fluor® 488 anti-human IgM |
|||
| GT09445 | Sony | 25 Tests | Ask for price |
Alexa Fluor® 488 anti-human CD4 |
|||
| GT09657 | Sony | 100 Tests | Ask for price |
Alexa Fluor® 488 anti-human CD8 |
|||
| GT12117 | Sony | 100 Tests | Ask for price |
Alexa Fluor® 488 anti-human CD3 |
|||
| GT12183 | Sony | 100 Tests | Ask for price |
Alexa Fluor® 488 anti-human IgD |
|||
| GT12456 | Sony | 100 Tests | Ask for price |
Alexa Fluor® 488 anti-human CD7 |
|||
| GT16066 | Sony | 25 Tests | Ask for price |
Alexa Fluor® 488 anti-mouse IgD |
|||
| GT17714 | Sony | 25 µg | Ask for price |
Alexa Fluor® 488 anti-mouse IgD |
|||
| GT17715 | Sony | 100 µg | Ask for price |
Alexa Fluor® 488 anti-mouse IgM |
|||
| GT17771 | Sony | 25 µg | Ask for price |
Alexa Fluor® 488 anti-mouse IgM |
|||
| GT17772 | Sony | 100 µg | Ask for price |
Alexa Fluor® 488 anti-mouse IgE |
|||
| GT17832 | Sony | 25 µg | Ask for price |
Alexa Fluor® 488 anti-mouse IgE |
|||
| GT17833 | Sony | 100 µg | Ask for price |
Alexa Fluor® 488 anti-Avi-tag |
|||
| GT18751 | Sony | 100 Tests | Ask for price |
Alexa Fluor® 488 anti-Avi-tag |
|||
| GT18752 | Sony | 100 µg | Ask for price |
Alexa Fluor® 488 anti-OCT4 (OCT3) |
|||
| GT19194 | Sony | 100 Tests | Ask for price |
Alexa Fluor® 488 anti-mouse CD3 |
|||
| GT00021 | Sony | 100 µg | Ask for price |
Alexa Fluor® 488 anti-mouse CD3 |
|||
| GT00022 | Sony | 25 µg | Ask for price |
Alexa Fluor® 488 anti-mouse CD5 |
|||
| GT00238 | Sony | 100 µg | Ask for price |
Alexa Fluor® 488 anti-human CD3 |
|||
| GT06695 | Sony | 100 Tests | Ask for price |
Alexa Fluor® 488 anti-human CD3 |
|||
| GT06727 | Sony | 100 Tests | Ask for price |
Alexa Fluor® 488 anti-human CD3 |
|||
| GT06756 | Sony | 100 µg | Ask for price |
Alexa Fluor® 488 anti-human IgM |
|||
| GT09446 | Sony | 100 Tests | Ask for price |
Alexa Fluor® 488 anti-human CD3 |
|||
| GT09600 | Sony | 100 Tests | Ask for price |
Alexa Fluor® 488 anti-human CD4 |
|||
| GT09656 | Sony | 25 Tests | Ask for price |
Alexa Fluor® 488 anti-human CD4 |
|||
| GT12032 | Sony | 100 Tests | Ask for price |
Alexa Fluor® 488 anti-human IgD |
|||
| GT12455 | Sony | 25 Tests | Ask for price |
Alexa Fluor® 488 anti-human CD7 |
|||
| GT16067 | Sony | 100 Tests | Ask for price |
Alexa Fluor® 488 anti-Avi-tag |
|||
| GT18750 | Sony | 25 Tests | Ask for price |
Alexa Fluor® 488 anti-Cyclin B1 |
|||
| GT19105 | Sony | 25 Tests | Ask for price |
Alexa Fluor® 488 anti-Cyclin B1 |
|||
| GT19106 | Sony | 100 Tests | Ask for price |
Alexa Fluor® 488 anti-mouse CD8a |
|||
| E16FMG008a-025U | EnoGene | 25 μg | EUR 130 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse CD8a |
|||
| E16FMG008a-100U | EnoGene | 100 μg | EUR 281.67 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse CD19 |
|||
| E16FMG019-025U | EnoGene | 25 μg | EUR 368.33 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse CD23 |
|||
| E16FMG023-100U | EnoGene | 100 μg | EUR 845 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse CD24 |
|||
| E16FMG024-025U | EnoGene | 25 μg | EUR 368.33 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse CD24 |
|||
| E16FMG024-100U | EnoGene | 100 μg | EUR 845 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse CD25 |
|||
| E16FMG025-025U | EnoGene | 25 μg | EUR 368.33 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse CD25 |
|||
| E16FMG025-100U | EnoGene | 100 μg | EUR 845 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse CD28 |
|||
| E16FMG028-025U | EnoGene | 25 μg | EUR 368.33 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse CD31 |
|||
| E16FMG031-025U | EnoGene | 25 μg | EUR 368.33 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse CD36 |
|||
| E16FMG036-025U | EnoGene | 25 μg | EUR 368.33 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse CD36 |
|||
| E16FMG036-100U | EnoGene | 100 μg | EUR 845 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse CD40 |
|||
| E16FMG040-100U | EnoGene | 100 μg | EUR 845 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse CD41 |
|||
| E16FMG041-025U | EnoGene | 25 μg | EUR 411.67 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse CD41 |
|||
| E16FMG041-100U | EnoGene | 100 μg | EUR 1061.67 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse CD45 |
|||
| E16FMG045-025U | EnoGene | 25 μg | EUR 368.33 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse CD45.1 |
|||
| E16FMG045.1-025U | EnoGene | 25 μg | EUR 368.33 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse CD45.2 |
|||
| E16FMG045.2-025U | EnoGene | 25 μg | EUR 368.33 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse CD48 |
|||
| E16FMG048-100U | EnoGene | 100 μg | EUR 845 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse CD54 |
|||
| E16FMG054-025U | EnoGene | 25 μg | EUR 368.33 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse CD68 |
|||
| E16FMG068-025U | EnoGene | 25 μg | EUR 541.67 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse CD68 |
|||
| E16FMG068-100U | EnoGene | 100 μg | EUR 1278.33 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse CD80 |
|||
| E16FMG080-025U | EnoGene | 25 μg | EUR 368.33 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse CD80 |
|||
| E16FMG080-100U | EnoGene | 100 μg | EUR 845 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse CD83 |
|||
| E16FMG083-025U | EnoGene | 25 μg | EUR 476.67 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse CD83 |
|||
| E16FMG083-100U | EnoGene | 100 μg | EUR 1191.67 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse CD86 |
|||
| E16FMG086-025U | EnoGene | 25 μg | EUR 368.33 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse CD86 |
|||
| E16FMG086-100U | EnoGene | 100 μg | EUR 845 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-mouse CD90.2 |
|||
| E16FMG090.2-025U | EnoGene | 25 μg | EUR 368.33 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-rat CD45RA |
|||
| E16FRG045RA-100U | EnoGene | 100 μg | EUR 845 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-human CD1a |
|||
| E16FHG001a-100 | EnoGene | 100 tests | EUR 975 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-human CD1c |
|||
| E16FHG001c-025 | EnoGene | 25 tests | EUR 498.33 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-human CD1c |
|||
| E16FHG001c-100 | EnoGene | 100 tests | EUR 1061.67 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-human CD8a |
|||
| E16FHG008a-025 | EnoGene | 25 tests | EUR 411.67 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-human CD8a |
|||
| E16FHG008a-100 | EnoGene | 100 tests | EUR 975 |
|
Description: Available in various conjugation types. |
|||
Alexa Fluor® 488 anti-human CD14 |
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| E16FHG0142-025 | EnoGene | 25 tests | EUR 325 |
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Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD14 |
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| E16FHG0142-100 | EnoGene | 100 tests | EUR 975 |
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Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD15 |
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| E16FHG0151-100 | EnoGene | 100 tests | EUR 975 |
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Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD16 |
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| E16FHG016-025 | EnoGene | 25 tests | EUR 455 |
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Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD16 |
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| E16FHG016-100 | EnoGene | 100 tests | EUR 975 |
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Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD19 |
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| E16FHG019-100 | EnoGene | 100 tests | EUR 975 |
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Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD20 |
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| E16FHG020-100 | EnoGene | 100 tests | EUR 975 |
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Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD24 |
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| E16FHG024-100 | EnoGene | 100 tests | EUR 975 |
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Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD25 |
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| E16FHG025-025 | EnoGene | 25 tests | EUR 455 |
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Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD25 |
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| E16FHG025-100 | EnoGene | 100 tests | EUR 975 |
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Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD28 |
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| E16FHG028-100 | EnoGene | 100 tests | EUR 975 |
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Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD29 |
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| E16FHG029-025 | EnoGene | 25 tests | EUR 455 |
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Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD29 |
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| E16FHG029-100 | EnoGene | 100 tests | EUR 975 |
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Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD31 |
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| E16FHG031-025 | EnoGene | 25 tests | EUR 455 |
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Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD31 |
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| E16FHG031-100 | EnoGene | 100 tests | EUR 975 |
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Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD34 |
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| E16FHG034-025 | EnoGene | 25 tests | EUR 455 |
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Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD34 |
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| E16FHG034-100 | EnoGene | 100 tests | EUR 975 |
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Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD38 |
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| E16FHG038-025 | EnoGene | 25 tests | EUR 455 |
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Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD38 |
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| E16FHG038-100 | EnoGene | 100 tests | EUR 975 |
|
Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD40 |
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| E16FHG040-100 | EnoGene | 100 tests | EUR 975 |
|
Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD45 |
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| E16FHG045-025 | EnoGene | 25 tests | EUR 411.67 |
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Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD45 |
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| E16FHG045-100 | EnoGene | 100 tests | EUR 975 |
|
Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD54 |
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| E16FHG054-025 | EnoGene | 25 tests | EUR 455 |
|
Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD54 |
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| E16FHG054-100 | EnoGene | 100 tests | EUR 975 |
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Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD64 |
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| E16FHG064-100 | EnoGene | 100 tests | EUR 975 |
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Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD68 |
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| E16FHG068-025 | EnoGene | 25 tests | EUR 455 |
|
Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD68 |
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| E16FHG068-100 | EnoGene | 100 tests | EUR 1061.67 |
|
Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD69 |
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| E16FHG069-100 | EnoGene | 100 tests | EUR 975 |
|
Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD80 |
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| E16FHG080-025 | EnoGene | 25 tests | EUR 455 |
|
Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD80 |
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| E16FHG080-100 | EnoGene | 100 tests | EUR 975 |
|
Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD83 |
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| E16FHG083-100 | EnoGene | 100 tests | EUR 1083.33 |
|
Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD86 |
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| E16FHG086-025 | EnoGene | 25 tests | EUR 455 |
|
Description: Available in various conjugation types. |
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Alexa Fluor® 488 anti-human CD86 |
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| E16FHG086-100 | EnoGene | 100 tests | EUR 975 |
|
Description: Available in various conjugation types. |
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Goat anti Mouse IgG1 (Alexa Fluor 488) |
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| 43R-1649 | Fitzgerald Industries International | 500 ug | Ask for price |
Alexa Fluor® 488 anti-mouse CD19 |
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| GT02200 | Sony | 100 µg | Ask for price |
Alexa Fluor® 488 anti-mouse CD19 |
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| GT02203 | Sony | 25 µg | Ask for price |
Alexa Fluor® 488 anti-mouse CD83 |
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| GT02859 | Sony | 25 µg | Ask for price |
Alexa Fluor® 488 anti-mouse CD28 |
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| GT02929 | Sony | 25 µg | Ask for price |
Alexa Fluor® 488 anti-mouse CD25 |
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| GT00543 | Sony | 100 µg | Ask for price |
Alexa Fluor® 488 anti-mouse CD31 |
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| GT00646 | Sony | 100 µg | Ask for price |
Alexa Fluor® 488 anti-mouse CD36 |
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| GT00695 | Sony | 100 µg | Ask for price |
Alexa Fluor® 488 anti-mouse CD38 |
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| GT00711 | Sony | 100 µg | Ask for price |
Our outcomes confirmed that ZAIS@PEI might mediate plasmid DNA supply into HeLa cells with the excessive transfection effectivity of 40% and low cytotoxicity, in the meantime permitting the real-time monitoring of gene transfection. The obtained ZAIS@PEI QDs had been verified as versatile self-tracking gene vectors, which built-in gene supply with bioimaging capabilities with out exterior labeling.