Short regulatory DNA sequences to target brain endothelial cells for gene therapy

Short regulatory DNA sequences to target brain endothelial cells for gene therapy

Gene vectors focusing on CNS endothelial cells enable to govern the blood-brain barrier and to right genetic defects within the CNS. As a result of vectors based mostly on the adeno-associated virus (AAV) have a restricted capability, it’s important that the DNA sequence controlling gene expression is brief. As well as, it should be particular for endothelial cells to keep away from off-target results.
To develop improved regulatory sequences with selectivity for mind endothelial cells, we examined the transcriptional exercise of truncated promoters of 11 (mind) endothelial-specific genes together with quick regulatory parts, i.e., the woodchuck post-transcriptional regulatory ingredient (W), the CMV enhancer ingredient (C), and a fraction of the primary intron of the Tie2 gene (S), by transfecting mind endothelial cells of three species.
4 mixtures of regulatory parts and quick promoters (Cdh5OclnSlc2a1, and Slco1c1) progressed by means of this in-vitro pipeline displaying appropriate exercise. When examined in mice, the regulatory sequences C-Ocln-W and C-Slc2a1-S-W enabled a stronger and extra particular gene expression in mind endothelial cells than the steadily used CAG promoter. In abstract, the brand new regulatory parts effectively management gene expression in mind endothelial cells and should assist to particularly goal the blood-brain barrier with gene remedy vectors.

From Pancreatic βCell Gene Networks to Novel Therapies for Kind 1 Diabetes

Completion of the Human Genome Mission enabled a novel systems- and network-level understanding of biology, however this stays to be utilized for understanding the pathogenesis of sort 1 diabetes (T1D). We suggest that defining the important thing gene regulatory networks that drive β-cell dysfunction and loss of life in T1D would possibly allow the design of therapies that focus on the core illness mechanism, specifically, the progressive lack of pancreatic β-cells.
Certainly, many profitable medicine don’t immediately goal particular person illness genes however, moderately, modulate the implications of faulty steps, focusing on proteins positioned one or two steps downstream. If we transpose this to the T1D scenario, it is smart to focus on the pathways that modulate the β-cell responses to the immune assault-in relation to indicators which will stimulate the immune response (e.g., HLA class I and chemokine overexpression and/or neoantigen expression) or inhibit the invading immune cells (e.g., PDL1 and HLA-E expression)-instead of focusing on solely the immune system, as it’s normally proposed.
Short regulatory DNA sequences to target brain endothelial cells for gene therapy
Right here we talk about the significance of a give attention to β-cells in T1D, classes realized from different autoimmune illnesses, the “different splicing connection,” information mining, and drug repurposing to guard β-cells in T1D after which among the preliminary candidates below testing for β-cell safety.

Danger evaluation of leachables in cell and gene remedy utilizing a CAR-T mannequin course of

With the quickly rising subject of autologous therapies, Single-Use (SU) applied sciences are more and more utilized in personalised medication resulting from their manifold benefits. Though qualification of the beginning materials of autologous therapies such because the CAR-T course of has been highlighted, little consideration has been paid to the impact of leachables on cell-based therapies, even when latest research point out interactions of leachables with cells.
To shut this hole, this examine presents a risk-analysis of SU-material on a CAR-T course of and identifies hazards imposed by tubing supplies and leachables thereof. With a purpose to symbolize a CAR-T course of in its entirety, two check programs, specifically a lentivirus manufacturing course of and first T-cells, had been used. Whereas the results on lentivirus manufacturing are similar to these reported for antibody manufacturing processes in CHO cells, we discovered that PVC materials and corresponding leachables, i.e. plasticizer, inhibit cell development of main T-cells to a fantastic extent. Moreover, our outcomes point out that important high quality attributes are affected by the PVC materials.

Engineering genetic units for in vivo management of therapeutic T cell exercise triggered by the dietary molecule resveratrol

Chimeric antigen receptor (CAR)-engineered T cell therapies have been acknowledged as highly effective methods in most cancers immunotherapy; nevertheless, the scientific software of CAR-T is at present constrained by extreme opposed results in sufferers, brought on by extreme cytotoxic exercise and poor T cell management. Herein, we harnessed a dietary molecule resveratrol (RES)-responsive transactivator and a transrepressor to develop a repressible transgene expression (RESrep) gadget and an inducible transgene expression (RESind) gadget, respectively.
After optimization, these instruments enabled the management of CAR expression and CAR-mediated antitumor operate in engineered human cells. We demonstrated {that a} resveratrol-repressible CAR expression (RESrep-CAR) gadget can successfully inhibit T cell activation upon resveratrol administration in main T cells and a xenograft tumor mouse mannequin.

T-Cell Immunoglobulin Mucin Receptor 1 (TIM-1) Antibody

abx415206-01mg 0.1 mg
EUR 439

T-Cell Immunoglobulin Mucin Receptor 3 (TIM-3) Antibody

abx415211-05mg 0.5 mg
EUR 829

T-Cell Immunoglobulin Mucin Receptor 3 (TIM-3) Antibody

abx415213-01mg 0.1 mg
EUR 439

T-Cell Immunoglobulin Mucin Receptor 4 (TIM-4) Antibody

abx415215-05mg 0.5 mg
EUR 829

T-Cell Immunoglobulin Mucin Receptor 4 (TIM-4) Antibody

abx415216-01mg 0.1 mg
EUR 439

T-Cell Immunoglobulin Mucin Receptor 1 (TIM-1) Antibody

abx433377-200ul 200 ul
EUR 384

T-Cell Immunoglobulin And Mucin Domain-Containing Protein 2 (TIM-2) Antibody (FITC)

abx415209-01mg 0.1 mg
EUR 509

Human T-Cell Immunoglobulin Mucin Receptor 3 (TIM-3) Antibody

32146-05111 150 ug
EUR 261

T-Cell Immunoglobulin Mucin Receptor 1 (TIM-1) Antibody (FITC)

abx415205-01mg 0.1 mg
EUR 509

T-Cell Immunoglobulin Mucin Receptor 1 (TIM-1) Antibody (RPE)

abx415207-100tests 100 tests
EUR 592

T-Cell Immunoglobulin Mucin Receptor 3 (TIM-3) Antibody (FITC)

abx415212-01mg 0.1 mg
EUR 509

T-Cell Immunoglobulin Mucin Receptor 3 (TIM-3) Antibody (RPE)

abx415214-100tests 100 tests
EUR 592

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Recombinant T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4)

4-RPH854Hu01
  • EUR 413.60
  • EUR 214.00
  • EUR 1276.00
  • EUR 492.00
  • EUR 884.00
  • EUR 340.00
  • EUR 3040.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Recombinant T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4) expressed in: E.coli

T-Cell immunoglobulin and mucin domain-Containing protein 4 (TIMD4) Antibody

20-abx318087
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

T-Cell immunoglobulin and mucin domain-Containing protein 4 (TIMD4) Antibody

abx031021-400ul 400 ul
EUR 523

T-Cell immunoglobulin and mucin domain-Containing protein 4 (TIMD4) Antibody

abx031021-80l 80 µl
EUR 286

T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4) Antibody

20-abx178542
  • EUR 1205.00
  • EUR 578.00
  • 1 mg
  • 200 ug

T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4) Antibody

20-abx174727
  • EUR 857.00
  • EUR 439.00
  • 1 mg
  • 200 ug

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric, Trial Size

CBA-135-T 24 assays
EUR 432
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

TIM3 (T-Cell Immunoglobulin)/HAVCR2

MO47039 100 ul
EUR 349

CytoSelect 96-Well Cell Transformation Assay (Cell Recovery Compatible, Fluorometric), Trial Size

CBA-140-T 24 assays
EUR 456
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

Human T-Cell Immunoglobulin Mucin Receptor 3 (TIM-3) Antibody (Biotin Conjugate)

32146-05121 150 ug
EUR 369

T-Cell immunoglobulin and mucin domain-Containing protein 4 (TIMD4) Antibody (HRP)

20-abx316455
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

T-Cell immunoglobulin and mucin domain-Containing protein 4 (TIMD4) Antibody (FITC)

20-abx316456
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

T-Cell immunoglobulin and mucin domain-Containing protein 4 (TIMD4) Antibody (Biotin)

20-abx316457
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Human T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4) Protein

20-abx655199
  • EUR 578.00
  • EUR 258.00
  • EUR 1720.00
  • EUR 690.00
  • EUR 425.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Recombinant Mouse T cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-6His)

CK57-10ug 10ug
EUR 110
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse T cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-6His)

CK57-1mg 1mg
EUR 1674
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse T cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-6His)

CK57-500ug 500ug
EUR 1186
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse T cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-6His)

CK57-50ug 50ug
EUR 232
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-Fc)

CM03-10ug 10ug
EUR 126
Description: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.

Recombinant Mouse T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-Fc)

CM03-1mg 1mg
EUR 1877
Description: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.

Recombinant Mouse T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-Fc)

CM03-500ug 500ug
EUR 1328
Description: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.

Recombinant Mouse T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-Fc)

CM03-50ug 50ug
EUR 232
Description: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.

Recombinant Mouse T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-6His)

CM54-10ug 10ug
EUR 126
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-6His)

CM54-1mg 1mg
EUR 1877
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-6His)

CM54-500ug 500ug
EUR 1328
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-6His)

CM54-50ug 50ug
EUR 232
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Rac2 Activation Assay Kit, Trial Size

STA-401-2-T 5 assays
EUR 403
Description: Our Rac Activation Assays use visible agarose beads to selectively precipitate the active form of Rac1 or Rac2. The precipitated small GTPase is then detected by Western blot using a Rac1- or Rac2-specific antibody included in the kit.

Rap2 Activation Assay Kit, Trial Size

STA-406-2-T 5 assays
EUR 403
Description: Our Rap Activation Assays use visible agarose beads to selectively precipitate the active form of Rap1 or Rap2. The precipitated small GTPase is then detected by Western blot using a Rap1- or Rap2-specific antibody included in the kit.

Human T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4) ELISA Kit

SEH854Hu-10x96wellstestplate 10x96-wells test plate
EUR 4731.5
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4) in tissue homogenates, cell lysates and other biological fluids.

Human T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4) ELISA Kit

SEH854Hu-1x48wellstestplate 1x48-wells test plate
EUR 477.3
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4) in tissue homogenates, cell lysates and other biological fluids.

Human T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4) ELISA Kit

SEH854Hu-1x96wellstestplate 1x96-wells test plate
EUR 639
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4) in tissue homogenates, cell lysates and other biological fluids.

Human T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4) ELISA Kit

SEH854Hu-5x96wellstestplate 5x96-wells test plate
EUR 2575.5
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4) in tissue homogenates, cell lysates and other biological fluids.

Human T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4) ELISA Kit

4-SEH854Hu
  • EUR 4782.00
  • EUR 2526.00
  • EUR 640.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Human T-cell immunoglobulin and mucin domain-containing protein 4(TIMD4) ELISA kit

CSB-EL023546HU-24T 1 plate of 24 wells
EUR 165
Description: Quantitativesandwich ELISA kit for measuring Human T-cell immunoglobulin and mucin domain-containing protein 4 (TIMD4) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human T-cell immunoglobulin and mucin domain-containing protein 4(TIMD4) ELISA kit

1-CSB-EL023546HU
  • EUR 804.00
  • EUR 5099.00
  • EUR 2704.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human T-cell immunoglobulin and mucin domain-containing protein 4(TIMD4) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 ELISA Kit (TIMD4)

RK02389 96 Tests
EUR 521

Human T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4) ELISA Kit

RD-TIMD4-Hu-48Tests 48 Tests
EUR 521

Human T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4) ELISA Kit

RD-TIMD4-Hu-96Tests 96 Tests
EUR 723

Human T-Cell Immunoglobulin Mucin Receptor 3 (TIM-3) AssayLite Antibody (FITC Conjugate)

32146-05141 150 ug
EUR 428

Human T-Cell Immunoglobulin Mucin Receptor 3 (TIM-3) AssayLite Antibody (RPE Conjugate)

32146-05151 150 ug
EUR 428

Human T-Cell Immunoglobulin Mucin Receptor 3 (TIM-3) AssayLite Antibody (APC Conjugate)

32146-05161 150 ug
EUR 428

Human T-Cell Immunoglobulin Mucin Receptor 3 (TIM-3) AssayLite Antibody (PerCP Conjugate)

32146-05171 150 ug
EUR 471

Human T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4) ELISA Kit

DLR-TIMD4-Hu-48T 48T
EUR 517
Description: A sandwich quantitative ELISA assay kit for detection of Human T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4) in samples from tissue homogenates, cell lysates or other biological fluids.

Human T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4) ELISA Kit

DLR-TIMD4-Hu-96T 96T
EUR 673
Description: A sandwich quantitative ELISA assay kit for detection of Human T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4) in samples from tissue homogenates, cell lysates or other biological fluids.

Human T-cell immunoglobulin and mucin domain-containing protein 4 (TIMD4) ELISA Kit

abx352339-96tests 96 tests
EUR 786

Human T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4) CLIA Kit

20-abx495562
  • EUR 7973.00
  • EUR 4246.00
  • EUR 981.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4) ELISA Kit

20-abx153299
  • EUR 7378.00
  • EUR 3933.00
  • EUR 911.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4) ELISA Kit

RDR-TIMD4-Hu-48Tests 48 Tests
EUR 544

Human T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4) ELISA Kit

RDR-TIMD4-Hu-96Tests 96 Tests
EUR 756

Recombinant Human T Cell Immunoglobulin and Mucin Domain-3/TIM3/HAVCR2 (C-6His)

C356-10ug 10ug
EUR 121
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.

Recombinant Human T Cell Immunoglobulin and Mucin Domain-3/TIM3/HAVCR2 (C-6His)

C356-1mg 1mg
EUR 2283
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.

Recombinant Human T Cell Immunoglobulin and Mucin Domain-3/TIM3/HAVCR2 (C-6His)

C356-500ug 500ug
EUR 1613
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.

Recombinant Human T Cell Immunoglobulin and Mucin Domain-3/TIM3/HAVCR2 (C-6His)

C356-50ug 50ug
EUR 263
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.

CytoSelect 24-Well Cell Migration Assay (8 µm, Colorimetric Format), Trial Size

CBA-100-T 4 assays
EUR 299
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines.

CytoSelect 24-Well Cell Migration Assay (8 µm, Fluorometric Format), Trial Size

CBA-101-T 4 assays
EUR 299
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines.

CytoSelect 24-Well Cell Migration Assay (5 µm, Fluorometric Format), Trial Size

CBA-102-T 4 assays
EUR 299
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 5 µm pore size is ideal for monocytes / macrophages.

CytoSelect 24-Well Cell Migration Assay (3 µm, Fluorometric Format), Trial Size

CBA-103-T 4 assays
EUR 299
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 3 µm pore size is best for the smallest cells including neutrophils and other leukocytes.

CytoSelect 24-Well Cell Invasion Assay (Basement Membrane, Colorimetric Format), Trial Size

CBA-110-T 4 assays
EUR 322
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with ECM matrix gel (basement membrane), a protein mix isolated from EHS tumor cells.

CytoSelect 24-Well Cell Invasion Assay (Basement Membrane, Fluorometric Format), Trial Size

CBA-111-T 4 assays
EUR 322
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with ECM matrix gel (basement membrane), a protein mix isolated from EHS tumor cells.

CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation), Trial Size

CBA-130-T 24 assays
EUR 386
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 349

Anti-Mucin 2 antibody

STJ16100978 1 mL
EUR 597

ELISA kit for Human TIMD4 (T-cell immunoglobulin and mucin domain-containing protein 4)

E-EL-H5515 1 plate of 96 wells
EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Human TIMD4 (T-cell immunoglobulin and mucin domain-containing protein 4) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human TIMD4 (T-Cell Immunoglobulin And Mucin Domain Containing Protein 4)

ELK4680 1 plate of 96 wells
EUR 432
Description: A sandwich ELISA kit for detection of T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Human T-cell immunoglobulin and mucin domain-containing protein 4 (TIMD4)

KTE62326-48T 48T
EUR 332
Description: Quantitative sandwich ELISA for measuring Human T-cell immunoglobulin and mucin domain-containing protein 4 (TIMD4) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human T-cell immunoglobulin and mucin domain-containing protein 4 (TIMD4)

KTE62326-5platesof96wells 5 plates of 96 wells
EUR 2115
Description: Quantitative sandwich ELISA for measuring Human T-cell immunoglobulin and mucin domain-containing protein 4 (TIMD4) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human T-cell immunoglobulin and mucin domain-containing protein 4 (TIMD4)

KTE62326-96T 96T
EUR 539
Description: Quantitative sandwich ELISA for measuring Human T-cell immunoglobulin and mucin domain-containing protein 4 (TIMD4) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Jurkat T / GFP Stable Cell (Flt1 Promoter)

SC049-2 2 x 106 cell/ml x 1ml
EUR 1500
Description: GFP expression stable cell line in human Jurkat T Cells with Puromycin resistance. GFP is expressed under the promoter of human Flt1 gene.

Recombinant Human T-cell Immunoglobulin and Mucin Domain-containing Protein 4/Tim-4 (C-6His)

CU18-10ug 10ug
EUR 141
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human T-cell Immunoglobulin and Mucin Domain-containing Protein 4/Tim-4 (C-6His)

CU18-1mg 1mg
EUR 1674
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human T-cell Immunoglobulin and Mucin Domain-containing Protein 4/Tim-4 (C-6His)

CU18-500ug 500ug
EUR 1186
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human T-cell Immunoglobulin and Mucin Domain-containing Protein 4/Tim-4 (C-6His)

CU18-50ug 50ug
EUR 303
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-Fc-6His)

CX71-10ug 10ug
EUR 121
Description: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.

Recombinant Human T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-Fc-6His)

CX71-1mg 1mg
EUR 2283
Description: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.

Recombinant Human T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-Fc-6His)

CX71-500ug 500ug
EUR 1613
Description: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.

Recombinant Human T Cell Immunoglobulin and Mucin Domain-3/TIM-3/HAVCR2 (C-Fc-6His)

CX71-50ug 50ug
EUR 263
Description: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.

anti-CD3 T-cell

501-A-01mg 0,1 mg
EUR 267.5
Description: anti-CD3 T-cell

anti-CD3 T-cell

501-A-1000ug 1000 ug
EUR 1282.5
Description: anti-CD3 T-cell

Anti-Adiponectin Receptor 2 (mouse) Antibody

A02218-2 200ug
EUR 498
Description: Goat Polyclonal Adiponectin Receptor 2 (mouse) Antibody. Validated in IF, IHC and tested in Mouse.

T-Pro Stacking Buffer

JB03-C002 500ml/BT
EUR 152

T-Pro MycoClean spray

JT90-R002 500ml/BT
EUR 144

Anti-T-Cell receptor antibody

STJ16100480 100 µg
EUR 406

OxiSelect Nitrotyrosine ELISA Kit, Trial Size

STA-305-T 32 assays
EUR 421
Description: Nitric oxide influences a variety of biological processes including cell proliferation, apoptosis, neurotoxicity and extracellular matrix remodeling. Nitric oxide reacts with superoxide to form peroxynitrite, which in turn nitrates tyrosine residues in proteins. Nitrotyrosine therefore serves as a marker for peroxynitrite action in a variety of disease states and in conditions of cellular damage and oxidative stress. Our OxiSelect Nitrotyrosine ELISA Kit provides a sensitive method to measure the formation of 3-nitrotyrosine in proteins.

OxiSelect Protein Carbonyl Fluorometric Assay, Trial Size

STA-307-T 20 assays
EUR 351
Description: The most common products of protein oxidation in biological samples are the carbonyl derivatives of proline, lysine, arginine and threonine residues. Such derivatives are chemically stable and serve as markers for oxidative stress in most types of reactive oxygen species. Our OxiSelect Protein Carbonyl Fluorometric Assay Kit provides a rapid, efficient method for the detection of protein carbonyl residues. The fluorescence plate-based format provides a convenient system for direct measurement of protein carbonyl content.

OxiSelect Protein Carbonyl Immunoblot Kit, Trial Size

STA-308-T 4 blots
EUR 264
Description: The most common products of protein oxidation in biological samples are the carbonyl derivatives of proline, lysine, arginine and threonine residues. Such derivatives are chemically stable and serve as markers for oxidative stress in most types of reactive oxygen species. Our OxiSelect Protein Carbonyl Immunoblot Kit provides a rapid, efficient method for the detection of protein carbonyl residues. The immunoblot format provides a convenient way to compare oxidized and non-oxidized protein fingerprints.

OxiSelect Protein Carbonyl ELISA Kit, Trial Size

STA-310-T 32 assays
EUR 421
Description: The most common products of protein oxidation in biological samples are the carbonyl derivatives of proline, lysine, arginine and threonine residues. Such derivatives are chemically stable and serve as markers for oxidative stress in most types of reactive oxygen species. Our OxiSelect Protein Carbonyl ELISA Kit provides a rapid, efficient method for the detection of protein carbonyl residues. The ELISA format is perfect for higher throughput and high sensitivity; we have eliminated concentration and precipitation steps allowing greater sample retention.

OxiSelect Total Glutathione (GSSG/GSH) Assay Kit, Trial Size

STA-312-T 20 assays
EUR 282
Description: The OxiSelect Total Glutathione Assay Kit is a quantitative assay for measuring the total glutathione content within a sample (GSH/GSSG).  Glutathione Reductase reduces oxidized glutathione (GSSG) to reduced glutathione (GSH) in the presence of NADPH.  Subsequently, the  chromogen reacts with the thiol group of GSH to produce a colored compound that absorbs at 405 nm.  The total glutathione content in unknown samples is determined by comparison with the predetermined glutathione standard curve.  The rate of chromophore production is proportional to the concentration of glutathione within the sample.  The rate can be determined from the absorbance change over time.  Metaphosphoric acid is provided to remove interfering proteins or enzymes from samples.

OxiSelect Oxidative DNA Damage ELISA Kit (8-OHdG Quantitation), Trial Size

STA-320-T 32 assays
EUR 508
Description: Among numerous types of oxidative DNA damage, 8-hydroxydeoxyguanosine (8-OHdG) is a ubiquitous marker of oxidative stress. 8-OHdG, one of the byproducts of oxidative DNA damage, is physiologically formed and enhanced by chemical carcinogens. Our OxiSelect Oxidative DNA Damage ELISA Kit (8-hydroxydeoxyguanosine assay) provides a powerful method for rapid, sensitive quantitation of 8-OHdG in DNA samples.

OxiSelect DNA Double Strand Break (DSB) Staining Kit, Trial Size

STA-321-T 20 assays
EUR 351
Description: Double-strand breaks (DSB) in DNA are among the most dangerous types of DNA damage occuring within cells. One of the earliest cellular responses to double-strand breaks is the phosphorylation of a histone variant, H2AX, at the sites of DNA damage. Within seconds Ser139 is phosphorylated when DSBs are induced in mammalian cells. Phosphorylation of this serine residue causes chromatin condensation and appears to play a critical role in the recruitment of repair or damage-signaling factors to the DNA damage sites. The OxiSelect DNA Double-Strand Break Staining Kit provides an easy-to-use method for detecting the presence of DSBs in cells cultured in microtiter plates. Double strand breaks can be detected in just a few hours by immunofluorescence staining of the phosphorylated histone H2AX.

OxiSelect UV-Induced DNA Damage ELISA Kit (CPD Quantitation), Trial Size

STA-322-T 32 assays
EUR 351
Description: Our OxiSelect UV-Induced DNA Damage ELISA Kit measures the formation of cyclobutane pyrimidine dimers (CPD) in DNA isolated from cells or tissues. Standards or unknown DNA samples are first heat denatured before adsorbed onto a 96-well DNA binding plate.  The sample or standard are then probed with an anti-CPD antibody, followed by an HRP conjugated secondary antibody. 

OxiSelect UV-Induced DNA Damage ELISA Kit (6-4PP Quantitation), Trial Size

STA-323-T 32 assays
EUR 351
Description: Our OxiSelect UV-Induced DNA Damage ELISA Kit measures 6-4PP formation in DNA isolated from cells or tissue. Standards or unknown DNA samples are first heat denatured before adsorbed onto a 96-well DNA binding plate.  The sample or standard is then probed with an anti-6-4PP antibody, followed by an HRP conjugated secondary antibody. 

OxiSelect Oxidative DNA Damage Quantitation Kit (AP Sites), Trial Size

STA-324-T 10 assays
EUR 415
Description: DNA damage can manifest in the formation of apurinic or apyrimidinic (AP or abasic) sites. Such spontaneous base loss in mammalian cells has been estimated at between 50,000 and 200,000 sites per day. Unrepaired abasic sites can inhibit transcription and may be mutagenic. Our OxiSelect Oxidative DNA Damage Quantitation Kit provides a simple, user-friendly method for the quantitaiton DNA damage in the form of abasic sites. The assay uses an Aldehyde Reactive Probe (ARP) to specifically react with an aldehyde group on the open ring of the AP site. This allows the AP site to be labeled with Biotin, followed by detection with Streptavidin-Enzyme conjugate.

OxiSelect Oxidative RNA Damage ELISA Kit (8-OHG Quantitation), Trial Size

STA-325-T 32 assays
EUR 508
Description: Recently oxidative RNA damage has been described in conjunction with a wide variety of neurological diseases including Alzheimer?s disease, Parkinson?s disease, Dementia with Lewy Bodies, prion disease, and subacute sclerosing panencephalitis. We have developed an easy-to-use ELISA for the detection of the most common marker of RNA damage: 8-hydroxyguanosine (8-OHG).

OxiSelect Cellular UV-Induced DNA Damage ELISA Kit (CPD), Trial Size

STA-326-T 32 assays
EUR 351
Description: Our OxiSelect Cellular UV-Induced DNA Damage ELISA Kit mesaures the formation of cyclobutane pyrimidine dimers (CPD) in intact cells. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-CPD antibody, followed by an HRP conjugated secondary antibody.

OxiSelect Cellular UV-Induced DNA Damage Staining Kit (CPD), Trial Size

STA-327-T 32 assays
EUR 351
Description: Our OxiSelect Cellular UV-Induced DNA Damage Staining Kit measures the formation of cyclobutane pyrimidine dimers (CPD) by immunofluorescence. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-CPD antibody, followed by a FITC conjugated secondary antibody. The unbound secondary antibody is removed during a wash step, and stained cells can then be visualized with a fluorescence microscope.

OxiSelect Cellular UV-Induced DNA Damage ELISA Kit (6-4PP), Trial Size

STA-328-T 32 assays
EUR 351
Description: Our OxiSelect Cellular UV-Induced DNA Damage ELISA Kit measures the formation of 6-4PP in intact cells in a 96-well ELISA plate-based format. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-6-4PP antibody, followed by an HRP conjugated secondary antibody. 

OxiSelect Cellular UV-Induced DNA Damage Staining Kit (6-4PP), Trial Size

STA-329-T 32 assays
EUR 351
Description: Our OxiSelect Cellular UV-Induced DNA Damage Staining Kit measures the formation of 6-4PP structures in DNA by immunofluorescence. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-6-4PP antibody, followed by a FITC conjugated secondary antibody. The unbound secondary antibody is removed during a wash step, and stained cells can then be visualized with a fluorescence microscope.

OxiSelect TBARS Assay Kit (MDA Quantitation), Trial Size

STA-330-T 20 assays
EUR 340
Description: The TBARS (Thiobarbituric Acid Reactive Substances) assay is well-established for screening and monitoring lipid peroxidation. MDA forms a 1:2 adduct with thiobarbituric acid; the MDA-TBA adduct can then be measured. Our OxiSelect TBARS Assay Kit provides a much more user-friendly protocol to measure the MDA-TBA adduct. Reaction volumes are much smaller than the traditional assay, so much less sample is required. Also, reactions can be performed in standard polypropylene tubes - no glass tubes or glass marbles are required. Important Note: MDA adducts are not stable long term. For best results test all samples immediately upon collection, or freeze them at -80ºC for up to one month. MDA may be degraded in samples that have been frozen for longer periods; in such cases more reliable results may be obtained from more stable markers of oxidative stress such as protein carbonyl, 8-OHdG or 4-HNE.

OxiSelect 8-iso-Prostaglandin F2a ELISA Kit, Trial Size

STA-337-T 32 assays
EUR 328
Description: 8-iso-Prostaglandin F2alpha (8-isoprostane) is a stable by-product of lipid peroxides generated during oxidative stress. Our OxiSelect 8-Isoprostane Assay Kit provides a convenient method for absolute quantitation in just a few hours. The 8-Isoprostane ELISA is suitable for a variety of sample types including urine, plasma, and cell lysates.

OxiSelect Catalase Activity Assay Kit, Fluorometric, Trial Size

STA-339-T 50 assays
EUR 398
Description: Catalase is a ubiquitous enzyme that destroys hydrogen peroxides formed during oxidative stress. Our OxiSelect Catalase Activity Assay Kit measures catalase activity in less than one hour from a variety of samples including blood, cells and tissues. Each kit provides sufficient reagents to perform up to 500 assays in a 96-well microtiter plate. This includes blanks, catalase standards and unknown samples. The OxiSelect Catalase Activity Assay Kit (Fluorometric) provides a 40-fold increase in sensitivity compared to our colorimetric assay.

OxiSelect Superoxide Dismutase Activity Assay, Trial Size

STA-340-T 20 assays
EUR 345
Description: Superoxide dismutase (SOD), which catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen, is one of the most important antioxidant enzymes. SOD enzymes are classified into three groups: cytosolic CuZn-SOD, mitochondrial Mn-SOD, and extracellular Ec-SOD. Our OxiSelect Superoxide Dismutase Activity Assay uses a xanthine/xanthine oxidase (XOD) system to generate superoxide anions and a chromagen to produce a water-soluble formazan dye upon reduction by superoxide anions. The superoxide dismutase activity is determined as the inhibition or reduction of chromagen.

OxiSelect Catalase Activity Assay Kit (Colorimetric), Trial Size

STA-341-T 20 assays
EUR 363
Description: Catalase is a ubiquitous enzyme that destroys hydrogen peroxides formed during oxidative stress. Our OxiSelect Catalase Activity Assay Kits measure catalase activity in less than one hour from a variety of samples including blood, cells and tissues. Each kit provides sufficient reagents to perform up to 100 assays in a 96-well microtiter plate, or 50 assays in microcentrifuge tubes. This includes blanks, catalase standards and unknown samples. Direct spectrophotometric detection of catalase activity with ultraviolet light can cause interference from proteins and other biological components. The OxiSelect Catalase Activity Assay Kit (Colorimetric) utilizes visible light (520 nm), which reduces sample interference. The OxiSelect Catalase Activity Assay Kit (Fluorometric) provides a 40-fold increase in sensitivity compared to our colorimetric assay.

OxiSelect Intracellular ROS Assay Kit (Green Fluorescence), Trial Size

STA-342-T 20 assays
EUR 345
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.

OxiSelect Hydrogen Peroxide/Peroxidase Assay Kit (Fluorometric), Trial Size

STA-344-T 50 assays
EUR 287
Description: The OxiSelect Hydrogen Peroxide/Peroxidase Assay Kit is a sensitive quantitative fluorometric assay for hydrogen peroxide or peroxidase.   In the presence of HRP, ADHP reacts with H2O2 in a 1:1 stoichiometry to produce highly fluorescent Resorufin.  The Resorufin product can be easily read by a fluorescence microplate reader with an excitation of 530-560 nm and an emission of 590 nm, or for absorbance at 560 nm.  Fluorescence values are proportional to the H2O2 or peroxidase levels within the samples.  The H2O2 or peroxidase content in unknown samples is determined by comparison with its respective standard curve.

OxiSelect Oxygen Radical Antioxidant Capacity (ORAC) Activity Assay, Trial Size

STA-345-T 48 assays
EUR 316
Description: The ORAC assay is a powerful tool to measure the antioxidant capacity of biomolecules. Our OxiSelect ORAC Activity Assay measures this capacity in less than 2 hours from a wide variety of sample types.

OxiSelect Hydroxyl Radical Antioxidant Capacity (HORAC) Activity Assay, Trial Size

STA-346-T 48 assays
EUR 316
Description: The HORAC assay is a powerful tool to measure the antioxidant capacity of biomolecules. Our OxiSelect HORAC Activity Assay measures the degradation of free hydroxyl radicals in less than 2 hours from a wide variety of sample types.

OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence), Trial Size

STA-347-T 20 assays
EUR 334
Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.

OxiSelect Total Antioxidant Capacity (TAC) Assay Kit, Trial Size

STA-360-T 20 assays
EUR 282
Description: The OxiSelect Total Antioxidant Capacity (TAC) Assay measures the total antioxidant capacity of biomolecules from a variety of samples via a SET mechanism. In the presence of antioxidants, copper(II) is reduced to copper(I). In turn, the copper(I) ions react with a chromogen to produce a color with maximum absorbance at 490nm.

Pan-Ras Activation Assay Kit, Trial Size

STA-400-T 5 assays
EUR 403
Description: Our Ras Activation Assays use visible agarose beads to selectively precipitate the active form of specific Ras protein of interest. The precipitated small GTPase is then detected by Western blot using a target-specific antibody included in the kit. Assays are available to detect specific isoforms H-Ras, K-Ras, and N-Ras, as well as a Pan-Ras assay that detects all three isoforms.
Moreover, we exhibit how a resveratrol-inducible CAR expression (RESind-CAR) gadget can obtain fine-tuned and reversible management over T cell activation through a resveratrol-titratable mechanism. Moreover, our outcomes revealed that the presence of RES can activate RESind-CAR T cells with robust anticancer cytotoxicity towards cells in vitro and in vivo. Our examine demonstrates the utility of RESrep and RESind units as efficient instruments for transgene expression and illustrates the potential of RESrep-CAR and RESind-CAR units to boost affected person security in precision most cancers immunotherapies.

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