Regardless of the unequivocal success of hematopoietic stem and progenitor cell gene remedy, limitations nonetheless exist together with genotoxicity and variegation/silencing of transgene expression. A category of DNA regulatory components often known as chromatin insulators (CI) can mitigate each, vector transcriptional silencing (barrier CIs) and vector-induced genotoxicity (enhancer-blocking CIs) and have been proposed as genetic modulators to attenuate undesirable vector-genome interactions. Just lately, plenty of human, small-sized and compact CIs bearing sturdy enhancer-blocking exercise had been recognized. To finally uncover an excellent CI with a twin, enhancer-blocking and barrier exercise, we interrogated these components in vitro and in vivo.
After preliminary screening of a collection of those enhancer-blocking insulators for potential barrier exercise, we recognized three distinct classes with no, partial or full safety towards transgene silencing. Subsequently, the 2 CIs with full barrier exercise (B4 and C1) had been examined for his or her potential to guard towards place results in main cells, after incorporation into lentiviral vectors and transduction of human CD34+ cells.
B4 and C1 didn’t adversely have an effect on vector titers resulting from their small dimension whereas they carried out as sturdy barrier insulators in CD34+cells, each in vitro and in vivo, shielding transgene’s long-term expression, extra robustly when positioned within the ahead orientation. Total, the incorporation of those dual-functioning components into therapeutic viral vectors will doubtlessly present a brand new era of safer and extra environment friendly lentiviral vectors for all hematopoietic stem cell gene remedy functions.
Single and double hit occasions in genes encoding for immune targets earlier than and after T cell participating antibody remedy in MM
T-cell participating immunotherapies exert unprecedented single-agent exercise in A number of Myeloma (MM), thereby placing a but unexplored selective stress on the clonal structure. On this examine, we report on homozygous BCMA (TNFRSF17) gene deletion after BCMA focusing on T-cell redirecting bispecific antibody remedy in a closely pretreated MM affected person. Lack of BCMA protein expression continued over subsequent relapses, with no response to anti-BCMA antibody drug conjugate (ADC) remedy.
In gentle of the a number of various targets that at the moment emerge along with BCMA, we prolonged our analyses to delineate a extra full image of genetic alterations that will influence immuno-therapy targets in MM. We carried out WGS and RNAseq in 100 MM sufferers (50 NDMM and 50 RRMM) and recognized a big proportion of sufferers with aberrations in genes encoding for immunotherapy targets, and GPRC5D ranked first with 15% heterozygous deletions, adopted by CD38 (10%), SDC1 (5%) and TNFRSF17 (4%).
Notably, these heterozygous deletions didn’t decrease the expression ranges of respective genes, however could symbolize a ‘first hit’ that drives the acquisition of homozygous deletions and, subsequent antigen-loss relapse upon focused immunotherapy. In abstract, we present pre-existing vulnerability in genes encoding for immuno-targets previous to and homozygous deletions after T-cell participating immunotherapy.
Leveraging the genetic variety of human stem cells in therapeutic approaches
Since their discovery 15 years in the past, human pluripotent stem cell (hPSC) applied sciences have begun to revolutionize science and drugs, quickly increasing past investigative analysis to drug discovery and growth. Efforts to leverage hPSCs over the past decade have centered on growing each the complexity and in vivo constancy of human mobile fashions by enhanced differentiation strategies.
Whereas these evolutions have fostered novel insights into illness mechanisms and influenced scientific drug discovery and growth, there are nonetheless a number of concerns that restrict the utility of hPSC fashions. On this overview, we spotlight essential, but underexplored avenues to broaden their attain. We concentrate on (i) the significance of diversifying current hPSC collections, and their utilization to research therapeutic methods in people from completely different genetic backgrounds, ancestry and intercourse; (ii) concerns for the choice of therapeutically related hPSC-based fashions; iii) methods to adequately improve the size of cell-based research; and iv) the advances and constraints of clinal trials in a dish.
Furthermore, we advocate for harnessing the translational capabilities of hPSC fashions together with using progressive, scalable approaches for understanding genetic biases and the influence of intercourse and ancestry on illness mechanisms and drug efficacy and response. The subsequent decade of hPSC innovation is poised to supply huge insights into the genetic foundation of human illness and allow speedy advances to develop, repurpose, and make sure the security of the subsequent era of illness therapies throughout numerous human populations.
Improvement and Characterization of Excessive Efficacy Cell-Penetrating Peptide through Modulation of the Histidine and Arginine Ratio for Gene Remedy
Cell-penetrating peptides (CPPs), as non-viral gene supply vectors, are thought of with decrease immunogenic response, and safer and better gene capability than viral programs. In our earlier examine, a CPP peptide referred to as RALA (arginine wealthy) introduced fascinating transfection efficacy and owns a possible clinic use. It’s believed that histidine may improve the endosome escaping potential of CPPs, but RALA peptide accommodates just one histidine in every chain.
With the intention to develop novel superior CPPs, through the use of RALA as a mannequin, we designed a collection of peptides named HALA (elevated histidine ratio). Each plasmid DNA (pDNA) and siRNA transfection outcomes on three cell traces revealed that the transfection efficacy is best when histidine replacements had been on the C-terminal as an alternative of on the N-terminal, and two histidine replacements are superior to 3.
 Antibody) PTPN11 (Ab-580) Antibody |
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| CSB-PA961746- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against PTPN11 (Ab-580). Recognizes PTPN11 (Ab-580) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200 |
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PTPN11 (Ab-580) Antibody |
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| CSB-PA961746-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against PTPN11 (Ab-580). Recognizes PTPN11 (Ab-580) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200 |
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 hsa-miR-580 Primers |
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| MPH01878 | ABM | 150 ul / 10 uM | EUR 121 |
 MemBriteâ„¢ Fix 568/580 |
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| 30095 | Biotium | 1KIT | EUR 644 |
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Description: Minimum order quantity: 1 unit of 1KIT |
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 Safflower yellow |
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| HY-N0938 | MedChemExpress | 50mg | EUR 133 |
 Antibody) Phospho-PYK2 (Tyr579/580) Antibody |
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| AF7383 | Affbiotech | 200ul | EUR 376 |
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Description: Phospho-PYK2 (Tyr579/580) Antibody detects endogenous levels of PYK2 only when phosphorylated at Tyr579/580. |
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) anti-SHP-2 (Ab-580) |
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| LF-PA20454 | Abfrontier | 100 ul | EUR 334 |
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Description: Rabbit polyclonal to SHP-2 |
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 hsa-miR-580 miRNA Inhibitor |
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| MIH03164 | ABM | 2 x 5.0 nmol | EUR 176 |
 hsa-miR-580 miRNA Antagomir |
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| MNH03164 | ABM | 2 x 5.0 nmol | EUR 329 |
 hsa-miR-580-3p Primers |
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| MPH03331 | ABM | 150 ul / 10 uM | EUR 121 |
 hsa-miR-580-5p Primers |
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| MPH03332 | ABM | 150 ul / 10 uM | EUR 121 |
 Antibody) PYK2 (Phospho-Tyr579/580) Antibody |
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| 13230-100ul | SAB | 100ul | EUR 252 |
PYK2 (Phospho-Tyr579/580) Antibody |
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| 13230-50ul | SAB | 50ul | EUR 187 |
 Antibody) SHP-2 (Ab-580) Antibody |
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| 21320-100ul | SAB | 100ul | EUR 252 |
SHP-2 (Ab-580) Antibody |
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| 21320-50ul | SAB | 50ul | EUR 187 |
 Anhydrosafflor yellow B |
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| TBW01543 | ChemNorm | 5mg | Ask for price |
 Safflor Yellow A |
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| TBZ2652 | ChemNorm | unit | Ask for price |
 Yellow Fluorescent Protein |
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| 4998-100 | Biovision | EUR 392 | |
Yellow Fluorescent Protein |
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| 4998-1000 | Biovision | EUR 2513 | |
Yellow Fluorescent Protein |
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| 4998-5000 | Biovision | EUR 6846 | |
 Hydroxysafflor yellow A |
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| HY-N0567 | MedChemExpress | 10mg | EUR 160 |
 Sunset Yellow FCF |
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| HY-D0249 | MedChemExpress | 100mg | EUR 108 |
 0.6ML MICROTUBES. YELLOW |
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| MCT-060-Y | CORNING | 1000/pk | EUR 183 |
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Description: Micro Tubes; Micro Tubes Push Cap- Axygen |
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 1.7ML MICROTUBES. YELLOW |
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| MCT-175-Y | CORNING | 500/pk | EUR 116 |
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Description: Micro Tubes; Micro Tubes Push Cap- Axygen |
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 Alizarin yellow GG |
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| AB0011 | Bio Basic | 25g | EUR 58.7 |
 Alizarin yellow R |
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| AB0012 | Bio Basic | 25g | EUR 58.7 |
 C.I.Vat Yellow 2 |
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| 20-abx181248 | Abbexa |
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 Pigment Yellow 42 |
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| abx185544-100g | Abbexa | 100 g | EUR 356 |
) Sunset Yellow (BSA) |
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| 20-abx165822 | Abbexa |
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) Sunset Yellow (OVA) |
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| 20-abx165823 | Abbexa |
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 200UL YELLOW TIPS |
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| T-200-Y | CORNING | 1000/pk | EUR 220 |
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Description: Non Filter Tips; Pipette Tips - Axygen |
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 Labeling Tape, Yellow |
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| TP004Y | Bio Basic | 1ROLLS, 1 UNIT | EUR 53.68 |
 Yellow Calibration Particles |
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| YCP-70-5 | Spherotech | 5 mL | EUR 324 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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 lucifer yellow cadaverine |
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| 80018 | Biotium | 25MG | EUR 149 |
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Description: Minimum order quantity: 1 unit of 25MG |
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 Naphthol Yellow S |
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| GT6579-100G | Glentham Life Sciences | 100 g | EUR 86 |
Naphthol Yellow S |
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| GT6579-250G | Glentham Life Sciences | 250 g | EUR 150 |
Naphthol Yellow S |
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| GT6579-25G | Glentham Life Sciences | 25 g | EUR 46 |
Sunset Yellow FCF |
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| GT7438-100G | Glentham Life Sciences | 100 g | EUR 110 |
Sunset Yellow FCF |
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| GT7438-25G | Glentham Life Sciences | 25 g | EUR 58 |
 Basic Yellow 40 |
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| GT8545-25G | Glentham Life Sciences | 25 g | EUR 86 |
 Alcian Yellow Solution |
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| ANY125 | ScyTek Laboratories | 125 ml | EUR 83 |
Alcian Yellow Solution |
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| ANY3800 | ScyTek Laboratories | 1 Gal. | EUR 454 |
Alcian Yellow Solution |
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| ANY500 | ScyTek Laboratories | 500 ml | EUR 123 |
Alcian Yellow Solution |
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| ANY999 | ScyTek Laboratories | 1000 ml | EUR 178 |
Hydroxysafflor yellow A |
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| N1526-20 | ApexBio | 20 mg | EUR 282 |
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Description: Extracted from Carthamus tinctorius L;Store the product in sealed,cool and dry condition |
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 Blocking Peptide) Phospho-PYK2 (Tyr579/580) Blocking Peptide |
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| AF7383-BP | Affbiotech | 1mg | EUR 195 |
 DAX-J2™ Ratio 580/460 |
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| 16310 | AAT Bioquest | 1 mg | EUR 393 |
 hsa-miR-580-3p miRNA Inhibitor |
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| MIH03165 | ABM | 2 x 5.0 nmol | EUR 176 |
 hsa-miR-580-5p miRNA Inhibitor |
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| MIH03166 | ABM | 2 x 5.0 nmol | EUR 176 |
 hsa-miR-580-3p miRNA Antagomir |
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| MNH03165 | ABM | 2 x 5.0 nmol | EUR 329 |
 hsa-miR-580-5p miRNA Antagomir |
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| MNH03166 | ABM | 2 x 5.0 nmol | EUR 329 |
 Antibody) Zinc Finger Protein 580 (ZNF580) Antibody |
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| abx030431-400ul | Abbexa | 400 ul | EUR 523 |
Zinc Finger Protein 580 (ZNF580) Antibody |
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| abx030431-80l | Abbexa | 80 µl | EUR 286 |
 MemBriteâ„¢ Fix 568/580, Trial Size |
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| 30095-T | Biotium | 1KIT | EUR 222 |
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Description: Minimum order quantity: 1 unit of 1KIT |
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 Conjugated Antibody) PYK2 (Phospho-Tyr579/580) Conjugated Antibody |
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| C13230 | SAB | 100ul | EUR 397 |
 Recombinant human Zinc finger protein 580 |
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| P2142 | FN Test | 100ug | Ask for price |
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Description: Recombinant protein for human Zinc finger protein 580 |
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 Hydroxy safflor Yellow A |
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| TB0782-1000 | ChemNorm | 20mg | EUR 207 |
 Antibody) Sunset Yellow (SY) Antibody |
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| 20-abx100868 | Abbexa |
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 Yellow Fever NS1 Antibody |
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| abx024098-1mg | Abbexa | 1 mg | EUR 773 |
Yellow Fever NS1 Antibody |
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| abx024122-1mg | Abbexa | 1 mg | EUR 773 |
Yellow Fever NS1 Antibody |
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| abx024123-1mg | Abbexa | 1 mg | EUR 773 |
Yellow Fever NS1 Antibody |
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| abx024124-1mg | Abbexa | 1 mg | EUR 773 |
Yellow Fever NS1 Antibody |
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| abx024125-1mg | Abbexa | 1 mg | EUR 773 |
Yellow Fever NS1 Antibody |
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| abx024126-1mg | Abbexa | 1 mg | EUR 773 |
Yellow Fever NS1 Antibody |
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| abx024127-1mg | Abbexa | 1 mg | EUR 773 |
 80-Well Rack, Yellow |
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| R567-Y | Bio Basic | 1 UNIT | EUR 52.51 |
 200UL YELLOW TIPS, RACKED |
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| T-200-Y-R | CORNING | 960/pk | EUR 125 |
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Description: Non Filter Tips; Pipette Tips - Axygen |
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 200UL YELLOW REFERENCE TIPS |
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| TR-222-Y | CORNING | 1000/pk | EUR 213 |
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Description: Non Filter Tips; Pipette Tips - Axygen |
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) Martius Yellow (C.I. 10315) |
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| GL3107-100G | Glentham Life Sciences | 100 g | EUR 198 |
Martius Yellow (C.I. 10315) |
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| GL3107-25G | Glentham Life Sciences | 25 g | EUR 90 |
 Fluorescent Light Yellow Particles |
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| FP-15045-2 | Spherotech | 2 mL | EUR 283 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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 YELLOW Lysis Kit 50 |
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| YELLOWE1 | Next Advance | 50pack | EUR 175 |
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Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. |
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 YELLOW Lysis Kit 250 |
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| YELLOWE5 | Next Advance | 250pack | EUR 548 |
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Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. |
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) Metanil Yellow (C.I. 13065) |
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| GT1665-100G | Glentham Life Sciences | 100 g | EUR 70 |
Metanil Yellow (C.I. 13065) |
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| GT1665-250G | Glentham Life Sciences | 250 g | EUR 110 |
Metanil Yellow (C.I. 13065) |
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| GT1665-50G | Glentham Life Sciences | 50 g | EUR 54 |
) Alcian Yellow (C.I. 12840) |
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| GT4246-1G | Glentham Life Sciences | 1 g | EUR 134 |
) Dimethyl Yellow (C.I. 11020) |
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| GT5992-100G | Glentham Life Sciences | 100 g | EUR 90 |
Dimethyl Yellow (C.I. 11020) |
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| GT5992-25G | Glentham Life Sciences | 25 g | EUR 54 |
Dimethyl Yellow (C.I. 11020) |
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| GT5992-500G | Glentham Life Sciences | 500 g | EUR 190 |
) Quinoline Yellow (C.I. 47000) |
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| GT7040-100G | Glentham Life Sciences | 100 g | EUR 118 |
Quinoline Yellow (C.I. 47000) |
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| GT7040-250G | Glentham Life Sciences | 250 g | EUR 198 |
Quinoline Yellow (C.I. 47000) |
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| GT7040-25G | Glentham Life Sciences | 25 g | EUR 62 |
) Nitrazine Yellow (C.I. 14890) |
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| GT9637-25G | Glentham Life Sciences | 25 g | EUR 213 |
Nitrazine Yellow (C.I. 14890) |
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| GT9637-5G | Glentham Life Sciences | 5 g | EUR 78 |
) Metanil Yellow (Aqueous Solution) |
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| MYQ500 | ScyTek Laboratories | 500 ml | EUR 98 |
Metanil Yellow (Aqueous Solution) |
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| MYQ999 | ScyTek Laboratories | 1000 ml | EUR 127 |
 Naphthol Yellow S Solution |
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| NYS125 | ScyTek Laboratories | 125 ml | EUR 101 |
Naphthol Yellow S Solution |
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| NYS500 | ScyTek Laboratories | 500 ml | EUR 170 |
Naphthol Yellow S Solution |
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| NYS999 | ScyTek Laboratories | 1000 ml | EUR 242 |
 Long trebler phosphoramidite |
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| A8151-250 | ApexBio | 250 mg | EUR 340 |
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Description: Long trebler phosphoramidite is a branching reagent for oligonucleotide synthesis allowing to synthesize branched DNA structures using standard DNA synthesizer. |
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, Colorimetric) CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric |
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| CBA-135 | Cell Biolabs | 96 assays | EUR 821 |
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Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis. |
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CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric |
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| CBA-135-5 | Cell Biolabs | 5 x 96 assays | EUR 3356 |
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Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis. |
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, Fluorometric) CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric |
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| CBA-140 | Cell Biolabs | 96 assays | EUR 856 |
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Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis. |
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CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric |
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| CBA-140-5 | Cell Biolabs | 5 x 96 assays | EUR 3483 |
|
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis. |
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 293LTV Cell Line |
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| LTV-100 | Cell Biolabs | 1 vial | EUR 508 |
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Description: The 293LTV Cell Line is derived from the parental 293 cells but selected for attributes that increase lentiviral production, including fimrer attachment and larger surface area. |
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 293AD Cell Line |
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| AD-100 | Cell Biolabs | 1 vial | EUR 461 |
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Description: The 293AD Cell Line is derived from the parental 293 cells but selected for attributes that increase adenovirus production, including firmer attachment and larger surface area. |
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 293AAV Cell Line |
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| AAV-100 | Cell Biolabs | 1 vial | EUR 508 |
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Description: The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production, including firmer attachment and larger surface area. |
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 293RTV Cell Line |
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| RV-100 | Cell Biolabs | 1 vial | EUR 508 |
|
Description: The 293RTV Cell Line is derived from the parental 293 cells but selected for attributes that increase retroviral production, including fimrer attachment and larger surface area. |
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) StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible) |
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| CBA-325 | Cell Biolabs | 96 assays | EUR 856 |
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Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis. |
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StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible) |
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| CBA-325-5 | Cell Biolabs | 5 x 96 assays | EUR 3361 |
|
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis. |
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 CORNING® CELL STRAINER, 100UM, YELLOW, STERILE, INDIVIDUALLY PACKAGED |
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| 431752 | CORNING | 1/pk | EUR 111 |
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Description: Lab Filtration; Corning Cell Strainers |
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 Human B Cell Leukemia X long ELISA kit |
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| E01B0575-192T | BlueGene | 192 tests | EUR 1270 |
|
Description: A competitive ELISA for quantitative measurement of Human B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human B Cell Leukemia X long ELISA kit |
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| E01B0575-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
|
Description: A competitive ELISA for quantitative measurement of Human B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human B Cell Leukemia X long ELISA kit |
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| E01B0575-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
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Description: A competitive ELISA for quantitative measurement of Human B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 Rat B Cell Leukemia X long ELISA kit |
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| E02B0575-192T | BlueGene | 192 tests | EUR 1270 |
|
Description: A competitive ELISA for quantitative measurement of Rat B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat B Cell Leukemia X long ELISA kit |
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| E02B0575-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
|
Description: A competitive ELISA for quantitative measurement of Rat B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat B Cell Leukemia X long ELISA kit |
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| E02B0575-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
|
Description: A competitive ELISA for quantitative measurement of Rat B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
 Mouse B Cell Leukemia X long ELISA kit |
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| E03B0575-192T | BlueGene | 192 tests | EUR 1270 |
|
Description: A competitive ELISA for quantitative measurement of Mouse B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse B Cell Leukemia X long ELISA kit |
|||
| E03B0575-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
|
Description: A competitive ELISA for quantitative measurement of Mouse B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Mouse B Cell Leukemia X long ELISA kit |
|||
| E03B0575-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
|
Description: A competitive ELISA for quantitative measurement of Mouse B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
 Rabbit B Cell Leukemia X long ELISA kit |
|||
| E04B0575-192T | BlueGene | 192 tests | EUR 1270 |
|
Description: A competitive ELISA for quantitative measurement of Rabbit B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Rabbit B Cell Leukemia X long ELISA kit |
|||
| E04B0575-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
|
Description: A competitive ELISA for quantitative measurement of Rabbit B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Rabbit B Cell Leukemia X long ELISA kit |
|||
| E04B0575-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
|
Description: A competitive ELISA for quantitative measurement of Rabbit B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
 Monkey B Cell Leukemia X long ELISA kit |
|||
| E09B0575-192T | BlueGene | 192 tests | EUR 1270 |
|
Description: A competitive ELISA for quantitative measurement of Monkey B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Monkey B Cell Leukemia X long ELISA kit |
|||
| E09B0575-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
|
Description: A competitive ELISA for quantitative measurement of Monkey B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Monkey B Cell Leukemia X long ELISA kit |
|||
| E09B0575-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
|
Description: A competitive ELISA for quantitative measurement of Monkey B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
 Pig B Cell Leukemia X long ELISA kit |
|||
| E07B0575-192T | BlueGene | 192 tests | EUR 1270 |
|
Description: A competitive ELISA for quantitative measurement of Porcine B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Pig B Cell Leukemia X long ELISA kit |
|||
| E07B0575-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
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Description: A competitive ELISA for quantitative measurement of Porcine B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig B Cell Leukemia X long ELISA kit |
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| E07B0575-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
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Description: A competitive ELISA for quantitative measurement of Porcine B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 Dog B Cell Leukemia X long ELISA kit |
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| E08B0575-192T | BlueGene | 192 tests | EUR 1270 |
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Description: A competitive ELISA for quantitative measurement of Canine B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog B Cell Leukemia X long ELISA kit |
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| E08B0575-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
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Description: A competitive ELISA for quantitative measurement of Canine B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog B Cell Leukemia X long ELISA kit |
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| E08B0575-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
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Description: A competitive ELISA for quantitative measurement of Canine B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 Goat B Cell Leukemia X long ELISA kit |
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| E06B0575-192T | BlueGene | 192 tests | EUR 1270 |
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Description: A competitive ELISA for quantitative measurement of Goat B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat B Cell Leukemia X long ELISA kit |
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| E06B0575-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
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Description: A competitive ELISA for quantitative measurement of Goat B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat B Cell Leukemia X long ELISA kit |
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| E06B0575-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
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Description: A competitive ELISA for quantitative measurement of Goat B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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, Colorimetric, Trial Size) CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric, Trial Size |
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| CBA-135-T | Cell Biolabs | 24 assays | EUR 432 |
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Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis. |
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 293/GFP Cell Line |
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| AKR-200 | Cell Biolabs | 1 vial | EUR 572 |
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Description: 293/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line. |
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 T47D/GFP Cell Line |
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| AKR-208 | Cell Biolabs | 1 vial | EUR 572 |
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Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line. |
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 A549/GFP Cell Line |
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| AKR-209 | Cell Biolabs | 1 vial | EUR 572 |
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Description: A549/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line. |
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 HeLa/GFP Cell Line |
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| AKR-213 | Cell Biolabs | 1 vial | EUR 572 |
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Description: HeLa/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line. |
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 NIH3T3/GFP Cell Line |
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| AKR-214 | Cell Biolabs | 1 vial | EUR 572 |
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Description: NIH3T3/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line. |
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 NIH3T3/Cas9 Cell Line |
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| AKR-5104 | Cell Biolabs | 1 vial | EUR 572 |
 293/Cas9 Cell Line |
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| AKR-5110 | Cell Biolabs | 1 vial | EUR 572 |
 HeLa/Cas9 Cell Line |
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| AKR-5111 | Cell Biolabs | 1 vial | EUR 572 |
, Trial Size) CytoSelect 96-Well Cell Transformation Assay (Cell Recovery Compatible, Fluorometric), Trial Size |
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| CBA-140-T | Cell Biolabs | 24 assays | EUR 456 |
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Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis. |
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 Collagen-based Cell Contraction Assay |
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| CBA-201 | Cell Biolabs | 24 assays | EUR 485 |
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Description: Cell Biolabs? Collagen-based Contraction Assay Kit provides a simple system to assess cell contractivity in vitro and screen cell contraction mediators. Each kit provides sufficient quantities to perform up to 24 assays in a 24-well plate. The kit can be also used in culturing cells in 3D collagen matrix. |
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 CytoSelect MTT Cell Proliferation Assay |
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| CBA-252 | Cell Biolabs | 960 assays | EUR 409 |
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Description: Cell Biolabs? CytoSelect MTT Cell Proliferation Assay provides a colorimetric format for measuring and monitoring cell proliferation. The kit contains sufficient reagents for the evaluation of 960 assays in 96-well plates or 192 assays in 24-well plates. Cells can be plated and then treated with compounds or agents that affect proliferation. Cells are then detected with the proliferation reagent, which is converted in live cells from the yellow tetrazole MTT to the purple formazan form by a cellular reductase (Figure 1). An increase in cell proliferation is accompanied by an increased signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions. The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues. This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells. |
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 SKOV-3/Luc Cell Line |
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| AKR-232 | Cell Biolabs | 1 vial | EUR 572 |
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Description: SKOV-3/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line. |
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 MCF-7/Luc Cell Line |
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| AKR-234 | Cell Biolabs | 1 vial | EUR 572 |
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Description: MCF-7/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line. |
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 OVCAR-5/RFP Cell Line |
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| AKR-254 | Cell Biolabs | 1 vial | EUR 572 |
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Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line. |
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 Embedding Cassettes |
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| M480-5-YELLOW | ScyTek Laboratories | 1 ea. | EUR 194 |
 Cassettes in QuickLoad Sleeves |
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| M480-5SL-YELLOW | ScyTek Laboratories | 1 ea. | EUR 284 |
 Yellow Fever Virus NS1 protein |
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| 80-1547 | Fitzgerald | 500 ug | EUR 1757 |
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Description: Purified Recombinant Yellow Fever Virus NS1 protein (His tag) |
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 mFluor™ Yellow 630 acid |
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| 1145 | AAT Bioquest | 5 mg | EUR 50 |
 mFluor™ Yellow 630 SE |
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| 1170 | AAT Bioquest | 1 mg | EUR 50 |
 Yellow fever virus Genome polyprotein |
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| 1-CSB-EP365905YAC1 | Cusabio |
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Description: Recombinant Yellow fever virus Genome polyprotein,partial expressed in E.coli |
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) BSA Conjugated Sunset Yellow (SY) |
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| 4-CPK523Ge11 | Cloud-Clone |
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Description: Recombinant Pan-species Sunset Yellow expressed in: chemical synthesis |
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) OVA Conjugated Sunset Yellow (SY) |
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| 4-CPK523Ge21 | Cloud-Clone |
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Description: Recombinant Pan-species Sunset Yellow expressed in: chemical synthesis |
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) UltraBrite? Yellow IHC chromogen (HRP) |
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| M1310-30 | Biovision | EUR 457 | |
 Labels On Roll, Yellow, 30X15mm |
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| LB004Y | Bio Basic | 1ROLLS, 1 UNIT | EUR 54.35 |
 Labels On Roll, Yellow, 40X20mm |
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| LB104Y | Bio Basic | 1ROLLS, 1 UNIT | EUR 54.42 |
 0.65ml Tube, Yellow, 1000/Bag |
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| BT613-Y | Bio Basic | 1PK, 1000UNIT | EUR 68.4 |
 Yellow fever virus PCR kit |
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| PCR-H627-48D | Bioingentech | 50T | EUR 453 |
Yellow fever virus PCR kit |
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| PCR-H627-96D | Bioingentech | 100T | EUR 572 |
 Yellow head virus PCR kit |
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| PCR-V294-48R | Bioingentech | 50T | EUR 823 |
Yellow head virus PCR kit |
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| PCR-V294-96R | Bioingentech | 100T | EUR 1113 |
 1.5ML BOIL-PROOF MICROTUBES. YELLOW |
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| MCT-150-Y | CORNING | 500/pk | EUR 110 |
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Description: Micro Tubes; Micro Tubes Push Cap- Axygen |
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 2.0ML BOIL-PROOF MICROTUBES. YELLOW |
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| MCT-200-Y | CORNING | 500/pk | EUR 116 |
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Description: Micro Tubes; Micro Tubes Push Cap- Axygen |
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 Antibody) Yellow Fever Virus (YFV) Antibody |
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| abx411683-01ml | Abbexa | 0.1 ml | EUR 509 |
Yellow Fever Virus (YFV) Antibody |
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| abx415761-01mg | Abbexa | 0.1 mg | EUR 592 |
 Yellow Fever Envelope Protein Protein |
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| abx160018-100ug | Abbexa | 100 ug | EUR 551 |
 Floating Microtube Racks Octagon, Yellow |
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| R021-Y | Bio Basic | 1 UNIT | EUR 51.7 |
 50-Well Microtube Racks, Yellow |
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| R022-Y | Bio Basic | 1 UNIT | EUR 53.05 |
 40-Well Microtube Racks, Yellow |
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| R027-Y | Bio Basic | 1 UNIT | EUR 53.05 |
 96-Well Rack, Reversible, Yellow |
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| R577-Y | Bio Basic | 1 UNIT | EUR 54.05 |
By investigating the mechanism of endocytosis of the pDNA nanocomplexes, we found that there have been a number of pathways that led to the method and caveolae performed the principle position. Through the screening, we found a novel peptide-HALA2 of excessive mobile transfection efficacy, which can act as an thrilling gene supply vector for gene remedy. Our findings additionally carry new insights on the event of novel strong CPPs.