Oligochitosan modified albumin as plasmid DNA delivery vector: endocytic trafficking, polyplex fate, in vivo compatibility.

Cationic macromolecules condense DNA into small nanoparticles and kind polyplex. The composition of the polyplex determines the endocytic course of, the intracellular routing and the destiny of the polyplex. Beforehand, oligochitosan-modified vectors with completely different protein moieties are used as gene supply vector and the varieties of protein moiety can affect the endosome escape capability and transfection effectivity. Among the many modified vectors, oligochitosan-modified bovine serum albumin (BSA) confirmed 90% transfection efficeincy in comparison with the modified zein and ovalbumin.

These knowledge inspired us to research the mechanism of internalization concerned within the superior transfection effectivity of modified BSA/ plasmid polyplex. The impact of particular endocytic inhibitors was studied in two adherent cell traces. The caveolae-mediated and lipid-mediated pathways play a big position within the polyplex internalization. Subsequent, a colocation of polyplex with lysosome was investigated within the presence of LysoTracker utilizing confocal microscopy.

As much as 70% of polyplex efficiently escaped the lysosome with out degradation. 4 non-adherent cell traces confirmed above than 60% transfection effectivity at an optimized vector/plasmid ratio. Furthermore, no important hemolytic impact was noticed as much as 500 μg/mL of cationic BSA, indicating no detectable cell membrane disruption. General, the hybrid biomacromolecule confirmed good intracellular supply and security in a mice mannequin.

Optimization of peptide-plasmid DNA vectors formulation for gene supply in most cancers remedy exploring design of experiments.

The sphere of gene remedy nonetheless attracts nice curiosity resulting from its potential therapeutic impact in the direction of probably the most lethal ailments, equivalent to most cancers. For most cancers gene remedy to be possible and viable in a scientific setting, the design and growth of an acceptable gene supply system is crucial. Peptide primarily based vectors, particularly, disclose to be promising for therapeutic gene launch. Following this, two completely different peptides, RALA and WRAP5, have been investigated primarily relating to their capability to kind complexes with a p53 encoding plasmid (pDNA) with appropriate properties for gene supply.

To deal with this problem, and after an preliminary screening research targeted on the dependence of pDNA complexation capability with the nitrogen to phosphate teams (N/P) ratio, a design of experiments (DoE) instrument has been employed. For every peptide/pDNA system, parameters equivalent to, the buffer pH and the N/P ratio have been thought of the DoE inputs and the vector dimension, zeta potential and pDNA complexation capability (CC) have been monitored as DoE outputs. The primary purpose was to search out the optimum experimental situations to attenuate particle sizes, in addition to, to maximise the optimistic floor fees of the formulated nanosystems and maximize the pDNA CC.

By means of the DoE methodology utilized, the optimum RALA/pDNA and WRAP5/pDNA formulations have been revealed and present attention-grabbing options associated to peptide construction and pDNA complexation capability. This work illustrates the nice utility of experimental design instruments in optimizing the formulation of peptide/pDNA vectors in a minimal variety of experiments offering related data for the event of extra appropriate and environment friendly gene supply methods. The brand new insights achieved on these carriers clearly instigate deeper analysis on gene remedy.

Novel Expression Vectors Based mostly on the pIGDM1 Plasmid.

Escherichia coli is likely one of the most generally used hosts for the manufacturing of heterologous proteins. Inside this host, the selection of cloning vector constitutes a key issue for a passable amplified expression of a goal gene. We aimed to develop novel, unpatented expression vectors that allow the steady upkeep and environment friendly overproduction of proteins in E. coli. A collection of expression vectors primarily based on the ColE1-like pIGDM1 plasmid have been constructed. The vectors named pIGDMCT7RS, pIGDM4RS and pIGDMKAN carry numerous antibiotic resistance genes: chloramphenicol, ampicillin or kanamycin, respectively. Two derivatives comprise the inducible T7 promoter whereas the third one bears the constitutive pms promoter from a scientific pressure of Klebsiella pneumoniae.

 

The pIGDM1-derivatives are appropriate with different ColE1-like plasmids generally utilized in molecular cloning. The pIGDMCT7RS and pIGDM4RS vectors comprise genes encoding AGA and AGG tRNAs, which complement the scarcity of those tRNAs, growing the effectivity of synthesis of heterologous proteins. In conclusion, pIGDMCT7RS, pIGDM4RS and pIGDMKAN vectors, with considerably improved options, together with compatibility with overwhelming majority of different plasmids, have been designed and constructed. They allow a high-level expression of a desired recombinant gene and due to this fact represent a possible, precious instrument for pharmaceutical corporations and analysis laboratories for their very own analysis or for the manufacturing of recombinant biopharmaceuticals.

Minimal plasmids play a necessary position in lots of intermediate steps in molecular biology. For instance, they can be utilized to assemble constructing blocks in artificial biology or be used as intermediate cloning plasmids that are perfect for PCR-based mutagenesis strategies. A small spine additionally opens up for added distinctive restriction enzyme cloning websites. Right here we describe the technology of pICOz, a 1185-bp absolutely useful high-copy cloning plasmid with an prolonged a number of cloning web site. We imagine that that is the smallest high-copy cloning vector ever described.

Single Plasmid-Based mostly, Upgradable, and Backward-Suitable Adenoviral Vector Programs.

Current adenoviral vector methods have two drawbacks. It’s labor-intensive and time-consuming to load a transgene in these methods, and transgene-harboring vectors are lifeless ends: they can’t be reused to assemble a vector carrying one other transgene or attaining new traits. To beat these shortcomings, single plasmid-based adenoviral vector methods have been constructed the place a singular PmeI web site was positioned on the place for insertion of the exogenous gene.

Human Glutamyl aminopeptidase (ENPEP)

1-CSB-RP147594h(c)
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Glutamyl aminopeptidase(ENPEP),partial expressed in E.coli

Saccharomyces cerevisiae Trehalose-phosphatase (TPS2)

1-CSB-RP165694Ye(c)
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Saccharomyces cerevisiae Trehalose-phosphatase(TPS2),partial expressed in E.coli

Mouse Protein Muc5ac (Muc5ac)

1-CSB-RP172294m(C)
  • EUR 606.00
  • EUR 318.00
  • EUR 2192.40
  • EUR 919.20
  • EUR 1461.60
  • EUR 402.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Mouse Protein Muc5ac(Muc5ac),partial expressed in E.coli

Human Histone deacetylase 7 (HDAC7)

1-CSB-RP178994h(C)
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Histone deacetylase 7(HDAC7),partial expressed in E.coli

Human Telomerase protein component 1 (TEP1)

1-CSB-RP180494h(c)
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Telomerase protein component 1(TEP1),partial expressed in E.coli

Human Collagen alpha-1 (XVII) chain (COL17A1)

1-CSB-RP180994h(c)
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Collagen alpha-1(XVII) chain(COL17A1),partial expressed in E.coli

Saccharomyces cerevisiae Neutral trehalase (NTH1)

1-CSB-RP181594Ye(c)
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Saccharomyces cerevisiae Neutral trehalase(NTH1),partial expressed in E.coli

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 418.8

Anti-c-MYC antibody

PAab01791 100 ug
EUR 494.4

anti- c-MYC antibody

FNab01791 100µg
EUR 702
Description: Antibody raised against c-MYC

Anti-c-Myc Antibody

PA1555 100ug/vial
EUR 352.8

Anti-c-Myc Antibody

PB9092 100ug/vial
EUR 400.8

Anti-c-Myc antibody

STJ119460 100 µl
EUR 332.4
Description: The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.

Anti-c-Myc antibody

STJ92355 200 µl
EUR 236.4
Description: Rabbit polyclonal to c-Myc.

Anti-c-Myc antibody

STJ92356 200 µl
EUR 236.4
Description: c-Myc is a protein encoded by the MYC gene which is approximately 48,8 kDa. c-Myc is localised to the nucleus. It is involved in signalling by NOTCH1, CDK-mediated phosphorylation and removal of Cdc6 and ERK signalling. It functions as a transcription factor that regulates transcription of specific target genes. It plays a role in cell cycle progression, apoptosis and cellular transformation. c-Myc is expressed in the nervous system, intestine, blood, liver and bone marrow. Mutations in the MYC gene may result in Burkitt lymphoma. STJ92356 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of c-Myc protein.

Anti-c-Myc antibody

STJ92357 200 µl
EUR 236.4
Description: Rabbit polyclonal to c-Myc.

Anti-c-Myc antibody

STJ92358 200 µl
EUR 236.4
Description: Rabbit polyclonal to c-Myc.

Anti-c-Myc antibody

STJ92359 200 µl
EUR 236.4
Description: Rabbit polyclonal to c-Myc.

Anti-c-Myc antibody

STJ97127 200 µl
EUR 236.4
Description: Rabbit polyclonal to c-Myc.

Anti-c-Myc antibody

STJ97962 100 µl
EUR 280.8
Description: Mouse monoclonal to c-Myc.

Anti-C-myc antibody

STJ160092 7 mL P
EUR 834

Anti-c-myc antibody

STJ180021 0.1 ml
EUR 282

Anti-c-Myc Monoclonal Antibody

M00026-1 100ug
EUR 476.4
Description: Rabbit Monoclonal c-Myc Antibody. Validated in WB and tested in Human, Mouse, Rat.

Anti-Myc Antibody

A00112 100ul
EUR 476.4
Description: Rabbit Polyclonal Myc Antibody. Validated in IHC and tested in Human, Mouse.

Anti-MYC antibody

STJ111071 100 µl
EUR 332.4
Description: The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.

Anti-MYC antibody

STJ113115 100 µl
EUR 332.4
Description: The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.

Anti-MYC antibody

STJ113455 50 µl
EUR 344.4
Description: The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.

Anti-MYC Antibody

STJ500591 100 µg
EUR 571.2

Anti-MYC Antibody

STJ500592 100 µg
EUR 571.2

Anti-myc antibody

STJ140011 150 µg
EUR 277.2
Description: Goat polyclonal antibody to myc epitope tag. c-Myc epitope tag is useful for the labelling and detection of proteins by immunoprecipitation, immunostaining and immunoblotting techniques. Because of its small size, it is unlikely to affect the tagged protein's biochemical properties.

c-Myc Epitope (Myc) Antibody

20-abx132269
  • EUR 526.80
  • EUR 159.60
  • EUR 1513.20
  • EUR 727.20
  • EUR 410.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

c-Myc Epitope (Myc) Antibody

20-abx130438
  • EUR 376.80
  • EUR 910.80
  • EUR 493.20
  • EUR 184.80
  • EUR 292.80
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

C-myc antibody

70R-10651 1 ml
EUR 516
Description: Affinity purified Chicken polyclonal C-myc antibody

C-Myc Antibody

6767-100
EUR 379.2

C-myc antibody

70-CC18 100 ug
EUR 397.2
Description: Affinity purified Chicken polyclonal C-myc antibody

c Myc antibody

10R-8403 100 ul
EUR 471.6
Description: Mouse monoclonal c Myc antibody

c-Myc antibody

70R-32862 100 ug
EUR 548.4
Description: Rabbit polyclonal c-Myc antibody

C-Myc Antibody

3995-100
EUR 392.4

C-Myc Antibody

3995-30T
EUR 175.2

C-myc Antibody

48044-100ul 100ul
EUR 399.6

C-myc Antibody

48044-50ul 50ul
EUR 286.8

C-myc antibody

20R-CC001 200 ug
EUR 457.2
Description: Chicken polyclonal C-myc antibody

C-myc antibody

10R-C101a 100 ug
EUR 651.6
Description: Mouse monoclonal C-myc antibody

C-myc antibody

70R-CG001 100 ul
EUR 457.2
Description: Affinity purified Goat polyclonal C-myc antibody

c-Myc Antibody

AF7674 200ul
EUR 540

c-Myc Antibody

AF6054 200ul
EUR 420

c-Myc Antibody

AF6055 200ul
EUR 420

c-Myc Antibody

AF0358 200ul
EUR 420

c-Myc Antibody

R30632 100 ug
EUR 419

c-Myc Antibody

RQ4860 100ul
EUR 419

c-Myc Antibody

RQ4861 100ul
EUR 419

c-Myc Antibody

F52056-0.08ML 0.08 ml
EUR 165

c-Myc Antibody

F52056-0.4ML 0.4 ml
EUR 389

c-Myc Antibody

F47610-0.08ML 0.08 ml
EUR 165

c-Myc Antibody

F47610-0.4ML 0.4 ml
EUR 379

c-Myc Antibody

RQ6663 100ug
EUR 419
Description: MYC proto-oncogene, bHLH transcription factor is a protein that in humans is encoded by the MYC gene which is a member of the myc family of transcription factors. The protein contains basic helix-loop-helix (bHLH) structural motif. This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.

c-Myc Antibody

V2744-100UG 100 ug
EUR 499
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This mAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V2744-20UG 20 ug
EUR 219
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This mAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V2744IHC-7ML 7 ml
EUR 499
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This mAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V2744SAF-100UG 100 ug
EUR 499
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This mAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V2745-100UG 100 ug
EUR 499
Description: Myc, or c-Myc, is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-Myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V2745-20UG 20 ug
EUR 219
Description: Myc, or c-Myc, is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-Myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V2745IHC-7ML 7 ml
EUR 499
Description: Myc, or c-Myc, is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-Myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V2745SAF-100UG 100 ug
EUR 499
Description: Myc, or c-Myc, is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-Myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V2746-100UG 100 ug
EUR 499
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. This mAb shows no cross-reaction with v-myc. c-Myc is a transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. [UniProt]

c-Myc Antibody

V2746-20UG 20 ug
EUR 219
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. This mAb shows no cross-reaction with v-myc. c-Myc is a transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. [UniProt]

c-Myc Antibody

V2746IHC-7ML 7 ml
EUR 499
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. This mAb shows no cross-reaction with v-myc. c-Myc is a transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. [UniProt]

c-Myc Antibody

V2746SAF-100UG 100 ug
EUR 499
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. This mAb shows no cross-reaction with v-myc. c-Myc is a transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. [UniProt]

c-Myc Antibody

V3124-100UG 100 ug
EUR 499
Description: The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

c-Myc Antibody

V3124-20UG 20 ug
EUR 219
Description: The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

c-Myc Antibody

V3124SAF-100UG 100 ug
EUR 499
Description: The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

c-Myc Antibody

V3125-100UG 100 ug
EUR 499
Description: The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

c-Myc Antibody

V3125-20UG 20 ug
EUR 219
Description: The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

c-Myc Antibody

V3125SAF-100UG 100 ug
EUR 499
Description: The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

c-Myc Antibody

V3158-100UG 100 ug
EUR 499
Description: The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

c-Myc Antibody

V3158-20UG 20 ug
EUR 219
Description: The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

c-Myc Antibody

V3158IHC-7ML 7 ml
EUR 499
Description: The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

c-Myc Antibody

V3158SAF-100UG 100 ug
EUR 499
Description: The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

c-Myc Antibody

V7021-100UG 100 ug
EUR 499
Description: Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. [UniProt]

c-Myc Antibody

V7021-20UG 20 ug
EUR 219
Description: Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. [UniProt]

c-Myc Antibody

V7021IHC-7ML 7 ml
EUR 499
Description: Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. [UniProt]

c-Myc Antibody

V7021SAF-100UG 100 ug
EUR 499
Description: Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. [UniProt]

c-Myc Antibody

V7971-100UG 100 ug
EUR 499
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V7971-20UG 20 ug
EUR 219
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V7971SAF-100UG 100 ug
EUR 499
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V8412-100UG 100 ug
EUR 499
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V8412-20UG 20 ug
EUR 219
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V8412SAF-100UG 100 ug
EUR 499
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V8454-100UG 100 ug
EUR 499
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V8454-20UG 20 ug
EUR 219
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V8454SAF-100UG 100 ug
EUR 499
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

anti-c-Myc

YF-PA13293 100 ul
EUR 483.6
Description: Rabbit polyclonal to c-Myc

pcDNA3.0-TO-Myc-RAR

PVTB00439-2a 2 ug
EUR 427.2

pCDNA4- To- Myc- HisB

PVT10694 2 ug
EUR 361.2

Anti-c-Myc Antibody (monoclonal, 9E10)

MA1028 100ug/vial
EUR 400.8

Anti-c-Myc Oncoprotein Monoclonal Antibody

M00026 100ug/vial
EUR 476.4
Description: Mouse Monoclonal c-Myc Oncoprotein Antibody. Validated in IHC and tested in Human.

Anti-Phospho-c-Myc (S373) antibody

STJ90227 200 µl
EUR 236.4
Description: Rabbit polyclonal to Phospho-c-Myc (S373).

Anti-Phospho-c-Myc (T358) antibody

STJ90228 200 µl
EUR 236.4
Description: Rabbit polyclonal to Phospho-c-Myc (T358).

Anti-Phospho-c-Myc (T58) antibody

STJ90229 200 µl
EUR 236.4
Description: Rabbit polyclonal to Phospho-c-Myc (T58).

Anti-Phospho-c-Myc (S62) antibody

STJ91021 200 µl
EUR 236.4
Description: Rabbit polyclonal to Phospho-c-Myc (S62).

Anti-C-Myc Antibody | CMYC-45A

CMYC-45A 1.0 mg
EUR 1142
Description:

Anti-C-Myc Antibody | CMYC-45A | Immunology Consultants Laboratory

Host: Chicken

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Polyclonal

Anti-C-Myc Antibody | CMYC-45B

CMYC-45B 1.0 mg
EUR 1157
Description:

Anti-C-Myc Antibody | CMYC-45B | Immunology Consultants Laboratory

Host: Chicken

Format: Biotin Conjugated

Product Type: Primary Antibody

Antibody Clonality: Polyclonal

Anti-C-Myc Antibody | CMYC-45F

CMYC-45F 1.0 mg
EUR 1157
Description:

Anti-C-Myc Antibody | CMYC-45F | Immunology Consultants Laboratory

Host: Chicken

Format: FITC Conjugated

Product Type: Primary Antibody

Antibody Clonality: Polyclonal

Anti-C-Myc Antibody | CMYC-45P

CMYC-45P 1.0 mg
EUR 1173
Description:

Anti-C-Myc Antibody | CMYC-45P | Immunology Consultants Laboratory

Host: Chicken

Format: HRP Conjugated

Product Type: Primary Antibody

Antibody Clonality: Polyclonal

Anti-C-Myc Antibody | GMYC-45A

GMYC-45A 0.1 mg
EUR 1142
Description:

Anti-C-Myc Antibody | GMYC-45A | Immunology Consultants Laboratory

Host: Goat

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Polyclonal

Anti-C-Myc Antibody | RMYC-145D

RMYC-145D 2.0 mL
EUR 1157
Description:

Anti-C-Myc Antibody | RMYC-145D | Immunology Consultants Laboratory

Host: Rabbit

Format: Agarose Immobilized

Product Type: Primary Antibody

Antibody Clonality: Polyclonal

Anti-C-Myc Antibody | RMYC-45A

RMYC-45A 1.0 mg
EUR 1142
Description:

Anti-C-Myc Antibody | RMYC-45A | Immunology Consultants Laboratory

Host: Rabbit

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Polyclonal

Anti-C-Myc Antibody | RMYC-45F

RMYC-45F 1.0 mg
EUR 1157
Description:

Anti-C-Myc Antibody | RMYC-45F | Immunology Consultants Laboratory

Host: Rabbit

Format: FITC Conjugated

Product Type: Primary Antibody

Antibody Clonality: Polyclonal

Anti-C-Myc Antibody | RMYC-45P

RMYC-45P 1.0 mg
EUR 1173
Description:

Anti-C-Myc Antibody | RMYC-45P | Immunology Consultants Laboratory

Host: Rabbit

Format: HRP Conjugated

Product Type: Primary Antibody

Antibody Clonality: Polyclonal

Human V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

DLR-MYC-Hu-48T 48T
EUR 597.6
Description: A sandwich quantitative ELISA assay kit for detection of Human V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) in samples from tissue homogenates, cell lysates or other biological fluids.

Human V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

DLR-MYC-Hu-96T 96T
EUR 776.4
Description: A sandwich quantitative ELISA assay kit for detection of Human V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) in samples from tissue homogenates, cell lysates or other biological fluids.

Mouse V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

DLR-MYC-Mu-48T 48T
EUR 609.6
Description: A sandwich quantitative ELISA assay kit for detection of Mouse V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) in samples from tissue homogenates, cell lysates or other biological fluids.

Mouse V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

DLR-MYC-Mu-96T 96T
EUR 793.2
Description: A sandwich quantitative ELISA assay kit for detection of Mouse V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) in samples from tissue homogenates, cell lysates or other biological fluids.

Rat V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

DLR-MYC-Ra-48T 48T
EUR 633.6
Description: A sandwich quantitative ELISA assay kit for detection of Rat V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids.

Rat V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

DLR-MYC-Ra-96T 96T
EUR 828
Description: A sandwich quantitative ELISA assay kit for detection of Rat V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids.

Human V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

RD-MYC-Hu-48Tests 48 Tests
EUR 600

Human V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

RD-MYC-Hu-96Tests 96 Tests
EUR 830.4

Mouse V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

RD-MYC-Mu-48Tests 48 Tests
EUR 613.2

Mouse V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

RD-MYC-Mu-96Tests 96 Tests
EUR 850.8

Rat V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

RD-MYC-Ra-48Tests 48 Tests
EUR 640.8

Rat V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

RD-MYC-Ra-96Tests 96 Tests
EUR 890.4

Human V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

RDR-MYC-Hu-48Tests 48 Tests
EUR 626.4

Human V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

RDR-MYC-Hu-96Tests 96 Tests
EUR 868.8

Mouse V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

RDR-MYC-Mu-48Tests 48 Tests
EUR 640.8

Mouse V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

RDR-MYC-Mu-96Tests 96 Tests
EUR 890.4

Rat V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

RDR-MYC-Ra-48Tests 48 Tests
EUR 669.6

Rat V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

RDR-MYC-Ra-96Tests 96 Tests
EUR 931.2

OR Antibody

44609-100ul 100ul
EUR 302.4

OR Antibody

44609-50ul 50ul
EUR 224.4

OR Antibody

ABD10250 100 ug
EUR 525.6

OR Antibody

20-abx217389
  • EUR 510.00
  • EUR 410.40
  • 100 ug
  • 50 ug

OR Antibody

DF10250 200ul
EUR 420

Anti-MYC-tag antibody

PAab05462 100 ug
EUR 426

anti- MYC tag antibody

FNab05456 100µg
EUR 658.5
Description: Antibody raised against MYC tag

anti- MYC-tag antibody

FNab05462 100µg
EUR 606.3
Description: Antibody raised against MYC-tag

Anti-Myc-Tag antibody

STJ96904 200 µl
EUR 236.4
Description: Mouse monoclonal to Myc-Tag.

Anti-Myc-Tag antibody

STJ96926 200 µl
EUR 236.4
Description: Mouse monoclonal to Myc-Tag.

Anti-Myc-Tag antibody

STJ96929 200 µl
EUR 236.4
Description: Mouse monoclonal to Myc-Tag.

Anti-Myc-Tag antibody

STJ97044 200 µl
EUR 236.4
Description: Mouse monoclonal to Myc-Tag.

Anti-Myc-Tag antibody

STJ97078 200 µl
EUR 236.4
Description: Rabbit polyclonal to Myc-Tag.

Anti-MYC Antibody (Biotin)

STJ500593 100 µg
EUR 703.2

Anti-MYC Antibody (FITC)

STJ500594 100 µg
EUR 703.2

Anti-MYC Antibody (Biotin)

STJ500595 100 µg
EUR 703.2

Anti-MYC Antibody (FITC)

STJ500596 100 µg
EUR 703.2

The polymerase chain response (PCR) amplified transgene could possibly be cloned into PmeI-linearized beginning plasmids utilizing one step of Gibson meeting to generate goal adenoviral plasmids, which have been then prepared for virus rescue. This process was termed restriction meeting.

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