The supercoiled round (SC) topology type of plasmid DNA has been regarded to be advantageous over open round or linearized analogue in transfection and expression effectivity, and due to this fact are largely demanded within the biopharmaceutical manufacturing. Nevertheless, manufacturing of high-purity SC plasmid DNA would lead to excessive manufacturing value. The impact of SC proportion in plasmid DNA on the standard of packaged lentiviral vectors has by no means been reported. On this research, we established an environment friendly system for manufacturing of high-titer lentiviral vectors utilizing suspension HEK293SF cells in serum-free media, and the lentiviral titer was not related to the proportion of SC plasmid DNA.
Plasmids DNA with totally different proportion of SC, open-circular, and linearized kinds have been ready utilizing the thermal denaturation technique, and have been transfected to adherent HEK293T or suspension HEK293SF cells for packaging of lentiviral vectors. The titer of lentiviral vectors from HEK293T cells, however not from HEK293SF cells, was considerably impaired when the proportion of SC plasmid DNA decreased from 60-80% to 30-40%. Additional lower of SC plasmid proportion to three% led to a dramatic discount of lentiviral titer irrespective of the packaging cell line was.
Nevertheless, lentiviral vectors from HEK293SF cells nonetheless confirmed a excessive titer even when the proportion of SC plasmid DNA was 3%. This research demonstrated that extraordinarily excessive proportion of SC plasmid DNA was not required for packaging of high-titer lentiviral vector in HEK293SF cells, a minimum of underneath our manufacturing course of.
Improvement of a Plasmid Shuttle Vector System for Genetic Manipulation of Chlamydia psittaci
The obligate intracellular bacterium Chlamydia psittaci is a identified avian pathogen inflicting psittacosis in birds and is able to zoonotic transmission. In human pulmonary infections, C. psittaci could cause pneumonia related to vital mortality if inadequately identified and handled. Though intracellular C. psittaci manipulates host cell organelles for its replication and survival, it has been troublesome to display host-pathogen interactions in C. psittaci an infection because of the lack of easy-to-handle genetic manipulation instruments. Right here, we present the genetic transformation of C. psittaci utilizing a plasmid shuttle vector that incorporates a controllable gene induction system.
The 7,553-bp plasmid p01DC12 was ready from the nonavian C. psittaci pressure 01DC12. We constructed the shuttle vector pCps-Tet-mCherry utilizing the complete sequence of p01DC12 and the 4,449-bp fragment of Chlamydia trachomatis shuttle vector pBOMB4-Tet-mCherry. pCps-Tet-mCherry contains genes encoding the inexperienced fluorescent protein (GFP), mCherry, and ampicillin resistance (AmpR). Goal genes will be inserted at a a number of cloning website (MCS). Importantly, these genes will be regulated by a tetracycline-inducible (tet) promoter.
Utilizing the pCps-Tet-mCherry plasmid shuttle vector, we present the expression of GFP, in addition to the induction of mCherry expression, in C. psittaci pressure 02DC15, which belongs to the avian C. psittaci 6BC clade. Moreover, we demonstrated that pCps-Tet-mCherry was stably retained in C. psittaci transformants. Thus, our C. psittaci plasmid shuttle vector system represents a novel focused method that allows the elucidation of host-pathogen interactions.
Psittacosis, brought on by avian C. psittaci, has a serious financial impression within the poultry business worldwide and represents a big danger for zoonotic transmission to people. Prior to now decade, the instruments of genetic manipulation have been improved for chlamydial molecular research. Whereas a number of genetic instruments have been primarily developed in Chlamydia trachomatis, a secure gene-inducible shuttle vector system has to not date been accessible for C. psittaci On this research, we tailored a C. trachomatis plasmid shuttle vector system to C. psittaci
We constructed a C. psittaci plasmid spine shuttle vector referred to as pCps-Tet-mCherry. The assemble expresses GFP in C. psittaci Importantly, exogeneous genes will be inserted at an MCS and are regulated by a tet promoter. The appliance of the pCps-Tet-mCherry shuttle vector system permits a promising new method to research unknown gene features of this pathogen.
Detection of A number of Transgene Fragments in a Mouse Mannequin of Gene Doping Primarily based on Plasmid Vector Utilizing TaqMan-qPCR Assay
The World Anti-Doping Company has prohibited gene doping within the context of progress in gene remedy. There’s a danger that the augmentation of genes utilizing plasmids may very well be utilized for gene doping. Nevertheless, no gold commonplace technique to detect this has been established. Right here, we aimed to develop a way to detect a number of transgene fragments as proof of gene doping. Firstly, gene supply mannequin mice as a mimic of gene doping have been created by injecting firefly luciferase plasmid with polyethylenimine (PEI) into the belly cavity.
The outcomes confirmed profitable institution of the mannequin, with ample luminescence upon in vivo imaging. Subsequent, a number of transgene fragments within the mannequin have been detected in plasma cell-free (cf)DNA, blood-cell-fraction DNA, and stool DNA utilizing the TaqMan- quantitative real-time PCR(qPCR) assay, with the very best ranges in plasma cfDNA. Utilizing only a single drop of complete blood from the mannequin, we additionally tried long-term detection.
 ELISA Kit) Mouse V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit |
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| RD-MYC-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 511 |
Mouse V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit |
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| RD-MYC-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 709 |
 ELISA Kit) Rat V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit |
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| RD-MYC-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 534 |
Rat V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit |
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| RD-MYC-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 742 |
 ELISA Kit) Human V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit |
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| DLR-MYC-Hu-48T | DL Develop | 48T | EUR 498 |
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Description: A sandwich quantitative ELISA assay kit for detection of Human V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) in samples from tissue homogenates, cell lysates or other biological fluids. |
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Human V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit |
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| DLR-MYC-Hu-96T | DL Develop | 96T | EUR 647 |
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Description: A sandwich quantitative ELISA assay kit for detection of Human V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) in samples from tissue homogenates, cell lysates or other biological fluids. |
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Mouse V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit |
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| DLR-MYC-Mu-48T | DL Develop | 48T | EUR 508 |
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Description: A sandwich quantitative ELISA assay kit for detection of Mouse V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) in samples from tissue homogenates, cell lysates or other biological fluids. |
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Mouse V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit |
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| DLR-MYC-Mu-96T | DL Develop | 96T | EUR 661 |
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Description: A sandwich quantitative ELISA assay kit for detection of Mouse V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) in samples from tissue homogenates, cell lysates or other biological fluids. |
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Rat V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit |
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| DLR-MYC-Ra-48T | DL Develop | 48T | EUR 528 |
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Description: A sandwich quantitative ELISA assay kit for detection of Rat V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids. |
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Rat V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit |
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| DLR-MYC-Ra-96T | DL Develop | 96T | EUR 690 |
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Description: A sandwich quantitative ELISA assay kit for detection of Rat V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids. |
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 Antibody) c-Myc Epitope (Myc) Antibody |
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| 20-abx132269 | Abbexa |
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c-Myc Epitope (Myc) Antibody |
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| 20-abx130438 | Abbexa |
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) c-Myc Oncoprotein; Clone 9E10.3 (Concentrate) |
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| RA0226-C.1 | ScyTek Laboratories | 0.1 ml | EUR 125 |
c-Myc Oncoprotein; Clone 9E10.3 (Concentrate) |
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| RA0226-C.5 | ScyTek Laboratories | 0.5 ml | EUR 300 |
) c-Myc Oncoprotein; Clone MYC909 (Concentrate) |
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| RA0227-C.1 | ScyTek Laboratories | 0.1 ml | EUR 125 |
c-Myc Oncoprotein; Clone MYC909 (Concentrate) |
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| RA0227-C.5 | ScyTek Laboratories | 0.5 ml | EUR 300 |
 C-myc antibody |
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| 70R-CG001 | Fitzgerald | 100 ul | EUR 381 |
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Description: Affinity purified Goat polyclonal C-myc antibody |
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 c-Myc antibody |
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| 70R-32862 | Fitzgerald | 100 ug | EUR 457 |
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Description: Rabbit polyclonal c-Myc antibody |
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 c-MYC Antibody |
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| AF0358 | Affbiotech | 200ul | EUR 304 |
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Description: MYC antibody detects endogenous levels of total MYC. |
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C-myc antibody |
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| 20R-CC001 | Fitzgerald | 200 ug | EUR 381 |
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Description: Chicken polyclonal C-myc antibody |
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C-myc antibody |
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| 70R-10651 | Fitzgerald | 1 ml | EUR 430 |
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Description: Affinity purified Chicken polyclonal C-myc antibody |
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C-myc antibody |
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| 70-CC18 | Fitzgerald | 100 ug | EUR 331 |
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Description: Affinity purified Chicken polyclonal C-myc antibody |
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 C-Myc Antibody |
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| 6767-100 | Biovision | EUR 316 | |
 c Myc antibody |
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| 10R-8403 | Fitzgerald | 100 ul | EUR 393 |
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Description: Mouse monoclonal c Myc antibody |
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C-myc antibody |
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| 10R-C101a | Fitzgerald | 100 ug | EUR 543 |
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Description: Mouse monoclonal C-myc antibody |
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C-Myc Antibody |
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| 3995-100 | Biovision | EUR 327 | |
C-Myc Antibody |
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| 3995-30T | Biovision | EUR 146 | |
 C-myc Antibody |
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| 48044-100ul | SAB | 100ul | EUR 333 |
C-myc Antibody |
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| 48044-50ul | SAB | 50ul | EUR 239 |
 c-Myc |
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| RA25085 | Neuromics | 100 ul | EUR 513 |
 C-Myc |
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| MYCF-100T | ImmunoStep | 100 test | EUR 505 |
 Antibody) Myc Proto-Oncogene Protein (c-Myc) Antibody |
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| 20-abx133153 | Abbexa |
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Myc Proto-Oncogene Protein (c-Myc) Antibody |
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| 20-abx133248 | Abbexa |
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Myc Proto-Oncogene Protein (c-Myc) Antibody |
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| 20-abx133250 | Abbexa |
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Myc Proto-Oncogene Protein (c-Myc) Antibody |
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| 20-abx133912 | Abbexa |
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Myc Proto-Oncogene Protein (c-Myc) Antibody |
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| 20-abx118000 | Abbexa |
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Myc Proto-Oncogene Protein (c-Myc) Antibody |
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| 20-abx137079 | Abbexa |
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Myc Proto-Oncogene Protein (c-Myc) Antibody |
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| abx037911-100ug | Abbexa | 100 ug | EUR 391 |
Myc Proto-Oncogene Protein (c-Myc) Antibody |
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| 20-abx008959 | Abbexa |
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Myc Proto-Oncogene Protein (c-Myc) Antibody |
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| 20-abx008960 | Abbexa |
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Myc Proto-Oncogene Protein (c-Myc) Antibody |
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| abx018354-100ug | Abbexa | 100 ug | EUR 495 |
Myc Proto-Oncogene Protein (c-Myc) Antibody |
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| abx022462-02mg | Abbexa | 0.2 mg | EUR 523 |
 Antibody) Myc Proto-Oncogene (c-Myc Peptide) Antibody |
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| abx022463-02mg | Abbexa | 0.2 mg | EUR 509 |
 Antibody) Myc Proto-Oncogene (C-Myc Peptide) Antibody |
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| abx023934-100ug | Abbexa | 100 ug | EUR 314 |
Myc Proto-Oncogene (C-Myc Peptide) Antibody |
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| abx023935-01ml | Abbexa | 0.1 ml | EUR 314 |
Myc Proto-Oncogene Protein (c-Myc) Antibody |
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| abx448567-100ug | Abbexa | 100 ug | EUR 523 |
Myc Proto-Oncogene Protein (c-Myc) Antibody |
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| abx448568-100ug | Abbexa | 100 ug | EUR 523 |
Myc Proto-Oncogene Protein (c-Myc) Antibody |
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| abx413504-01mg | Abbexa | 0.1 mg | EUR 634 |
Myc Proto-Oncogene Protein (c-Myc) Antibody |
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| abx413821-1mg | Abbexa | 1 mg | EUR 829 |
Myc Proto-Oncogene Protein (c-Myc) Antibody |
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| abx413825-2mg | Abbexa | 2 mg | EUR 1525 |
Myc Proto-Oncogene Protein (c-Myc) Antibody |
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| abx413826-01mg | Abbexa | 0.1 mg | EUR 411 |
Myc Proto-Oncogene Protein (c-Myc) Antibody |
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| abx413829-20ug | Abbexa | 20 ug | EUR 272 |
 Antibody) Myc Proto-Oncogene Protein (c-MYC) Antibody |
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| abx231791-100ug | Abbexa | 100 ug | EUR 551 |
Myc Proto-Oncogene Protein (c-Myc) Antibody |
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| abx139766-01mg | Abbexa | 0.1 mg | EUR 384 |
) Monoclonal MYC / c-Myc Antibody (aa408-420) |
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| AMM02198G | Leading Biology | 0.05mg | EUR 484 |
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Description: A Monoclonal antibody against Human MYC / c-Myc (aa408-420). The antibodies are raised in Mouse. This antibody is applicable in IHC-P, E |
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) Polyclonal MYC / c-Myc Antibody (aa25-74) |
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| APR02915G | Leading Biology | 0.05ml | EUR 484 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human MYC / c-Myc (aa25-74). This antibody is tested and proven to work in the following applications: |
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) Polyclonal MYC / c-Myc Antibody (aa386-435) |
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| APR02947G | Leading Biology | 0.05ml | EUR 484 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human MYC / c-Myc (aa386-435). This antibody is tested and proven to work in the following applications: |
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 control) Human recombinant purified His-tag c-myc protein (~65 kda) control |
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| MYC17-C | Alpha Diagnostics | 100 ul | EUR 286 |
 C-Myc-Tag Antibody |
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| T0001 | Affbiotech | 1ml | EUR 920 |
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Description: N/A. |
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 C-MYC-tag Antibody |
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| T0052 | Affbiotech | 1ml | EUR 920 |
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Description: The C-Myc tag antibody can detect C-Myc fusion proteins. |
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) C-myc antibody (FITC) |
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| 60R-1085 | Fitzgerald | 1 ml | EUR 397 |
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Description: Affinity purified Chicken polyclonal C-myc antibody (FITC) |
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C-myc antibody (FITC) |
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| 60R-CG001FT | Fitzgerald | 100 ug | EUR 489 |
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Description: Goat polyclonal C-myc antibody (FITC) conjugated |
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) C-myc antibody (HRP) |
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| 60R-CG001hrp | Fitzgerald | 100 ug | EUR 478 |
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Description: Goat polyclonal C-myc antibody (HRP) conjugated |
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 Anti-c-MYC antibody |
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| PAab01791 | Lifescience Market | 100 ug | EUR 412 |
 c-Myc Polyclonal Antibody |
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| ES6307-100ul | ELK Biotech | 100ul | EUR 279 |
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Description: A Rabbit Polyclonal antibody against c-Myc from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA |
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c-Myc Polyclonal Antibody |
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| ES6307-50ul | ELK Biotech | 50ul | EUR 207 |
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Description: A Rabbit Polyclonal antibody against c-Myc from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA |
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c-Myc Polyclonal Antibody |
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| ES6308-100ul | ELK Biotech | 100ul | EUR 279 |
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Description: A Rabbit Polyclonal antibody against c-Myc from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA |
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c-Myc Polyclonal Antibody |
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| ES6308-50ul | ELK Biotech | 50ul | EUR 207 |
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Description: A Rabbit Polyclonal antibody against c-Myc from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA |
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 Anti-c-Myc Antibody |
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| PA1555 | BosterBio | 100ug/vial | EUR 294 |
Anti-c-Myc Antibody |
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| PB9092 | BosterBio | 100ug/vial | EUR 334 |
c-Myc Polyclonal Antibody |
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| ES2009-100ul | ELK Biotech | 100ul | EUR 279 |
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Description: A Rabbit Polyclonal antibody against c-Myc from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA |
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c-Myc Polyclonal Antibody |
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| ES2009-50ul | ELK Biotech | 50ul | EUR 207 |
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Description: A Rabbit Polyclonal antibody against c-Myc from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA |
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c-Myc Polyclonal Antibody |
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| ES2010-100ul | ELK Biotech | 100ul | EUR 279 |
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Description: A Rabbit Polyclonal antibody against c-Myc from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA |
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c-Myc Polyclonal Antibody |
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| ES2010-50ul | ELK Biotech | 50ul | EUR 207 |
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Description: A Rabbit Polyclonal antibody against c-Myc from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA |
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c-Myc Polyclonal Antibody |
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| ES2011-100ul | ELK Biotech | 100ul | EUR 279 |
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Description: A Rabbit Polyclonal antibody against c-Myc from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA |
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c-Myc Polyclonal Antibody |
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| ES2011-50ul | ELK Biotech | 50ul | EUR 207 |
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Description: A Rabbit Polyclonal antibody against c-Myc from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA |
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c-Myc Polyclonal Antibody |
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| ES1182-100ul | ELK Biotech | 100ul | EUR 279 |
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Description: A Rabbit Polyclonal antibody against c-Myc from Human. This antibody is tested and validated for WB, ELISA |
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c-Myc Polyclonal Antibody |
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| ES1182-50ul | ELK Biotech | 50ul | EUR 207 |
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Description: A Rabbit Polyclonal antibody against c-Myc from Human. This antibody is tested and validated for WB, ELISA |
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c-Myc Polyclonal Antibody |
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| EA053-100ul | ELK Biotech | 100ul | EUR 279 |
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Description: A Rabbit Polyclonal antibody against c-Myc from Human. This antibody is tested and validated for WB, ELISA, IHC |
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c-Myc Polyclonal Antibody |
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| EA053-50ul | ELK Biotech | 50ul | EUR 207 |
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Description: A Rabbit Polyclonal antibody against c-Myc from Human. This antibody is tested and validated for WB, ELISA, IHC |
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 Antibody) c-Myc (pT358) Antibody |
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| 20-abx133249 | Abbexa |
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c-Myc Polyclonal Antibody |
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| ABP55308-003ml | Abbkine | 0.03ml | EUR 158 |
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Description: A polyclonal antibody for detection of c-Myc from Human, Mouse, Rat. This c-Myc antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human c-Myc around the non-phosphorylation site of T58 |
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c-Myc Polyclonal Antibody |
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| ABP55308-01ml | Abbkine | 0.1ml | EUR 289 |
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Description: A polyclonal antibody for detection of c-Myc from Human, Mouse, Rat. This c-Myc antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human c-Myc around the non-phosphorylation site of T58 |
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c-Myc Polyclonal Antibody |
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| ABP55308-02ml | Abbkine | 0.2ml | EUR 414 |
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Description: A polyclonal antibody for detection of c-Myc from Human, Mouse, Rat. This c-Myc antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human c-Myc around the non-phosphorylation site of T58 |
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c-Myc Polyclonal Antibody |
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| ABP55309-003ml | Abbkine | 0.03ml | EUR 158 |
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Description: A polyclonal antibody for detection of c-Myc from Human, Mouse, Rat. This c-Myc antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human c-Myc around the non-phosphorylation site of S373 |
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c-Myc Polyclonal Antibody |
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| ABP55309-01ml | Abbkine | 0.1ml | EUR 289 |
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Description: A polyclonal antibody for detection of c-Myc from Human, Mouse, Rat. This c-Myc antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human c-Myc around the non-phosphorylation site of S373 |
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c-Myc Polyclonal Antibody |
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| ABP55309-02ml | Abbkine | 0.2ml | EUR 414 |
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Description: A polyclonal antibody for detection of c-Myc from Human, Mouse, Rat. This c-Myc antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human c-Myc around the non-phosphorylation site of S373 |
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 Antibody) c-Myc (pT58) Antibody |
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| 20-abx009775 | Abbexa |
|
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 Antibody) c-Myc (pS62) Antibody |
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| 20-abx009776 | Abbexa |
|
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c-Myc Polyclonal Antibody |
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| ABP51010-003ml | Abbkine | 0.03ml | EUR 158 |
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Description: A polyclonal antibody for detection of c-Myc from Human, Mouse, Rat. This c-Myc antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human c-Myc around the non-phosphorylation site of T358 |
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c-Myc Polyclonal Antibody |
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| ABP51010-01ml | Abbkine | 0.1ml | EUR 289 |
|
Description: A polyclonal antibody for detection of c-Myc from Human, Mouse, Rat. This c-Myc antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human c-Myc around the non-phosphorylation site of T358 |
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c-Myc Polyclonal Antibody |
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| ABP51010-02ml | Abbkine | 0.2ml | EUR 414 |
|
Description: A polyclonal antibody for detection of c-Myc from Human, Mouse, Rat. This c-Myc antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human c-Myc around the non-phosphorylation site of T358 |
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c-Myc Polyclonal Antibody |
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| ABP51011-003ml | Abbkine | 0.03ml | EUR 158 |
|
Description: A polyclonal antibody for detection of c-Myc from Human, Mouse, Rat. This c-Myc antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human c-Myc at AA range: 360-440 |
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c-Myc Polyclonal Antibody |
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| ABP51011-01ml | Abbkine | 0.1ml | EUR 289 |
|
Description: A polyclonal antibody for detection of c-Myc from Human, Mouse, Rat. This c-Myc antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human c-Myc at AA range: 360-440 |
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c-Myc Polyclonal Antibody |
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| ABP51011-02ml | Abbkine | 0.2ml | EUR 414 |
|
Description: A polyclonal antibody for detection of c-Myc from Human, Mouse, Rat. This c-Myc antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human c-Myc at AA range: 360-440 |
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c-Myc Polyclonal Antibody |
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| ABP51012-003ml | Abbkine | 0.03ml | EUR 158 |
|
Description: A polyclonal antibody for detection of c-Myc from Human, Mouse, Rat. This c-Myc antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human c-Myc around the non-phosphorylation site of S62 |
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c-Myc Polyclonal Antibody |
|||
| ABP51012-01ml | Abbkine | 0.1ml | EUR 289 |
|
Description: A polyclonal antibody for detection of c-Myc from Human, Mouse, Rat. This c-Myc antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human c-Myc around the non-phosphorylation site of S62 |
|||
c-Myc Polyclonal Antibody |
|||
| ABP51012-02ml | Abbkine | 0.2ml | EUR 414 |
|
Description: A polyclonal antibody for detection of c-Myc from Human, Mouse, Rat. This c-Myc antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human c-Myc around the non-phosphorylation site of S62 |
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 Antibody) c-Myc(MYC275) Antibody |
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| BNCA0275-250 | Biotium | 250uL | EUR 383 |
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Description: Primary antibody against c-Myc(MYC275), APC conjugate, Concentration: 0.1mg/mL |
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 Antibody) c-Myc(9E10.) Antibody |
|||
| BNCA0596-250 | Biotium | 250uL | EUR 383 |
|
Description: Primary antibody against c-Myc(9E10.), APC conjugate, Concentration: 0.1mg/mL |
|||
 Antibody) c-Myc(MYC699) Antibody |
|||
| BNCA0699-250 | Biotium | 250uL | EUR 383 |
|
Description: Primary antibody against c-Myc(MYC699), APC conjugate, Concentration: 0.1mg/mL |
|||
 Antibody) c-Myc(MYC909) Antibody |
|||
| BNCA0909-250 | Biotium | 250uL | EUR 383 |
|
Description: Primary antibody against c-Myc(MYC909), APC conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC275) Antibody |
|||
| BNCAP0275-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against c-Myc(MYC275), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC275) Antibody |
|||
| BNCAP0275-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against c-Myc(MYC275), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(9E10.) Antibody |
|||
| BNCAP0596-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against c-Myc(9E10.), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(9E10.) Antibody |
|||
| BNCAP0596-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against c-Myc(9E10.), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC699) Antibody |
|||
| BNCAP0699-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against c-Myc(MYC699), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC699) Antibody |
|||
| BNCAP0699-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against c-Myc(MYC699), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC909) Antibody |
|||
| BNCAP0909-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against c-Myc(MYC909), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC909) Antibody |
|||
| BNCAP0909-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against c-Myc(MYC909), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC275) Antibody |
|||
| BNC810275-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against c-Myc(MYC275), CF680R conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC275) Antibody |
|||
| BNC810275-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against c-Myc(MYC275), CF680R conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(9E10.) Antibody |
|||
| BNC810596-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against c-Myc(9E10.), CF680R conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(9E10.) Antibody |
|||
| BNC810596-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against c-Myc(9E10.), CF680R conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC699) Antibody |
|||
| BNC810699-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against c-Myc(MYC699), CF680R conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC699) Antibody |
|||
| BNC810699-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against c-Myc(MYC699), CF680R conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC909) Antibody |
|||
| BNC810909-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against c-Myc(MYC909), CF680R conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC909) Antibody |
|||
| BNC810909-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against c-Myc(MYC909), CF680R conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC275) Antibody |
|||
| BNC880275-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against c-Myc(MYC275), CF488A conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC275) Antibody |
|||
| BNC880275-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against c-Myc(MYC275), CF488A conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(9E10.) Antibody |
|||
| BNC880596-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against c-Myc(9E10.), CF488A conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(9E10.) Antibody |
|||
| BNC880596-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against c-Myc(9E10.), CF488A conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC699) Antibody |
|||
| BNC880699-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against c-Myc(MYC699), CF488A conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC699) Antibody |
|||
| BNC880699-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against c-Myc(MYC699), CF488A conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC275) Antibody |
|||
| BNCB0275-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against c-Myc(MYC275), Biotin conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC275) Antibody |
|||
| BNCB0275-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against c-Myc(MYC275), Biotin conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(9E10.) Antibody |
|||
| BNCB0596-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against c-Myc(9E10.), Biotin conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(9E10.) Antibody |
|||
| BNCB0596-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against c-Myc(9E10.), Biotin conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC699) Antibody |
|||
| BNCB0699-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against c-Myc(MYC699), Biotin conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC699) Antibody |
|||
| BNCB0699-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against c-Myc(MYC699), Biotin conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC909) Antibody |
|||
| BNCB0909-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against c-Myc(MYC909), Biotin conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC909) Antibody |
|||
| BNCB0909-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against c-Myc(MYC909), Biotin conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC275) Antibody |
|||
| BNC550275-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against c-Myc(MYC275), CF555 conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC275) Antibody |
|||
| BNC550275-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against c-Myc(MYC275), CF555 conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(9E10.) Antibody |
|||
| BNC550596-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against c-Myc(9E10.), CF555 conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(9E10.) Antibody |
|||
| BNC550596-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against c-Myc(9E10.), CF555 conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC699) Antibody |
|||
| BNC550699-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against c-Myc(MYC699), CF555 conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC699) Antibody |
|||
| BNC550699-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against c-Myc(MYC699), CF555 conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC909) Antibody |
|||
| BNC550909-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against c-Myc(MYC909), CF555 conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC909) Antibody |
|||
| BNC550909-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against c-Myc(MYC909), CF555 conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC275) Antibody |
|||
| BNC430275-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against c-Myc(MYC275), CF543 conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC275) Antibody |
|||
| BNC430275-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against c-Myc(MYC275), CF543 conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(9E10.) Antibody |
|||
| BNC430596-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against c-Myc(9E10.), CF543 conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(9E10.) Antibody |
|||
| BNC430596-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against c-Myc(9E10.), CF543 conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC699) Antibody |
|||
| BNC430699-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against c-Myc(MYC699), CF543 conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC699) Antibody |
|||
| BNC430699-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against c-Myc(MYC699), CF543 conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC909) Antibody |
|||
| BNC430909-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against c-Myc(MYC909), CF543 conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC909) Antibody |
|||
| BNC430909-500 | Biotium | 500uL | EUR 544 |
|
Description: Primary antibody against c-Myc(MYC909), CF543 conjugate, Concentration: 0.1mg/mL |
|||
c-Myc(MYC275) Antibody |
|||
| BNC470275-100 | Biotium | 100uL | EUR 199 |
|
Description: Primary antibody against c-Myc(MYC275), CF647 conjugate, Concentration: 0.1mg/mL |
|||
The outcomes confirmed that a number of transgene fragments have been detected till 11 days. These findings point out that the mix of plasma cfDNA or only one drop of complete blood with TaqMan-qPCR assay is possible to detect plasmid-PEI-based gene doping. Our findings may speed up the event of strategies for detecting gene doping in people.