Production of lentiviral vectors in suspension cells using low proportion of supercoiled circular plasmid DNA

Production of lentiviral vectors in suspension cells using low proportion of supercoiled circular plasmid DNA

The supercoiled round (SC) topology type of plasmid DNA has been regarded to be advantageous over open round or linearized analogue in transfection and expression effectivity, and due to this fact are largely demanded within the biopharmaceutical manufacturing. Nevertheless, manufacturing of high-purity SC plasmid DNA would lead to excessive manufacturing value. The impact of SC proportion in plasmid DNA on the standard of packaged lentiviral vectors has by no means been reported. On this research, we established an environment friendly system for manufacturing of high-titer lentiviral vectors utilizing suspension HEK293SF cells in serum-free media, and the lentiviral titer was not related to the proportion of SC plasmid DNA.

Plasmids DNA with totally different proportion of SC, open-circular, and linearized kinds have been ready utilizing the thermal denaturation technique, and have been transfected to adherent HEK293T or suspension HEK293SF cells for packaging of lentiviral vectors. The titer of lentiviral vectors from HEK293T cells, however not from HEK293SF cells, was considerably impaired when the proportion of SC plasmid DNA decreased from 60-80% to 30-40%. Additional lower of SC plasmid proportion to three% led to a dramatic discount of lentiviral titer irrespective of the packaging cell line was.

Nevertheless, lentiviral vectors from HEK293SF cells nonetheless confirmed a excessive titer even when the proportion of SC plasmid DNA was 3%. This research demonstrated that extraordinarily excessive proportion of SC plasmid DNA was not required for packaging of high-titer lentiviral vector in HEK293SF cells, a minimum of underneath our manufacturing course of.

Improvement of a Plasmid Shuttle Vector System for Genetic Manipulation of Chlamydia psittaci

The obligate intracellular bacterium Chlamydia psittaci is a identified avian pathogen inflicting psittacosis in birds and is able to zoonotic transmission. In human pulmonary infections, C. psittaci could cause pneumonia related to vital mortality if inadequately identified and handled. Though intracellular C. psittaci manipulates host cell organelles for its replication and survival, it has been troublesome to display host-pathogen interactions in C. psittaci an infection because of the lack of easy-to-handle genetic manipulation instruments. Right here, we present the genetic transformation of C. psittaci utilizing a plasmid shuttle vector that incorporates a controllable gene induction system.

The 7,553-bp plasmid p01DC12 was ready from the nonavian C. psittaci pressure 01DC12. We constructed the shuttle vector pCps-Tet-mCherry utilizing the complete sequence of p01DC12 and the 4,449-bp fragment of Chlamydia trachomatis shuttle vector pBOMB4-Tet-mCherry. pCps-Tet-mCherry contains genes encoding the inexperienced fluorescent protein (GFP), mCherry, and ampicillin resistance (AmpR). Goal genes will be inserted at a a number of cloning website (MCS). Importantly, these genes will be regulated by a tetracycline-inducible (tet) promoter.

Utilizing the pCps-Tet-mCherry plasmid shuttle vector, we present the expression of GFP, in addition to the induction of mCherry expression, in C. psittaci pressure 02DC15, which belongs to the avian C. psittaci 6BC clade. Moreover, we demonstrated that pCps-Tet-mCherry was stably retained in C. psittaci transformants. Thus, our C. psittaci plasmid shuttle vector system represents a novel focused method that allows the elucidation of host-pathogen interactions.

Psittacosis, brought on by avian C. psittaci, has a serious financial impression within the poultry business worldwide and represents a big danger for zoonotic transmission to people. Prior to now decade, the instruments of genetic manipulation have been improved for chlamydial molecular research. Whereas a number of genetic instruments have been primarily developed in Chlamydia trachomatis, a secure gene-inducible shuttle vector system has to not date been accessible for C. psittaci On this research, we tailored a C. trachomatis plasmid shuttle vector system to C. psittaci

Production of lentiviral vectors in suspension cells using low proportion of supercoiled circular plasmid DNA

We constructed a C. psittaci plasmid spine shuttle vector referred to as pCps-Tet-mCherry. The assemble expresses GFP in C. psittaci Importantly, exogeneous genes will be inserted at an MCS and are regulated by a tet promoter. The appliance of the pCps-Tet-mCherry shuttle vector system permits a promising new method to research unknown gene features of this pathogen.

Detection of A number of Transgene Fragments in a Mouse Mannequin of Gene Doping Primarily based on Plasmid Vector Utilizing TaqMan-qPCR Assay

The World Anti-Doping Company has prohibited gene doping within the context of progress in gene remedy. There’s a danger that the augmentation of genes utilizing plasmids may very well be utilized for gene doping. Nevertheless, no gold commonplace technique to detect this has been established. Right here, we aimed to develop a way to detect a number of transgene fragments as proof of gene doping. Firstly, gene supply mannequin mice as a mimic of gene doping have been created by injecting firefly luciferase plasmid with polyethylenimine (PEI) into the belly cavity.

The outcomes confirmed profitable institution of the mannequin, with ample luminescence upon in vivo imaging. Subsequent, a number of transgene fragments within the mannequin have been detected in plasma cell-free (cf)DNA, blood-cell-fraction DNA, and stool DNA utilizing the TaqMan- quantitative real-time PCR(qPCR) assay, with the very best ranges in plasma cfDNA. Utilizing only a single drop of complete blood from the mannequin, we additionally tried long-term detection.

c-Myc(MYC275) Antibody

BNC800275-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC275), CF680 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNC800699-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC699), CF680 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNC800699-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC699), CF680 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNC810275-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC275), CF680R conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNC810275-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC275), CF680R conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNC810699-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC699), CF680R conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNC810699-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC699), CF680R conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNC810909-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC909), CF680R conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNC810909-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC909), CF680R conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNC940275-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC275), CF594 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNC940275-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC275), CF594 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNC940699-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC699), CF594 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNC940699-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC699), CF594 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNC940909-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC909), CF594 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNC940909-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC909), CF594 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNC700275-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC275), CF770 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNC700275-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC275), CF770 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNC700699-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC699), CF770 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNC700699-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC699), CF770 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNC700909-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC909), CF770 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNC700909-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC909), CF770 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNC880699-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC699), CF488A conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNC880699-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC699), CF488A conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNC880909-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC909), CF488A conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNC880909-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC909), CF488A conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNC050699-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC699), CF405M conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNC050699-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC699), CF405M conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNC050909-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC909), CF405M conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNC050909-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC909), CF405M conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNC040699-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC699), CF405S conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNC040699-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC699), CF405S conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNC040909-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC909), CF405S conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNC040909-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC909), CF405S conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNC430275-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC275), CF543 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNC430275-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC275), CF543 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNC400275-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC275), CF640R conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNC400275-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC275), CF640R conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNC400699-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC699), CF640R conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNC400699-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC699), CF640R conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNC400909-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC909), CF640R conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNC400909-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC909), CF640R conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNC050275-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC275), CF405M conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNC050275-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC275), CF405M conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNCB0275-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC275), Biotin conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNCB0275-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC275), Biotin conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNCB0699-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC699), Biotin conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNCB0699-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC699), Biotin conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNCAP0909-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC909), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNCAP0909-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC909), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNCA0909-250 250uL
EUR 459.6
Description: Primary antibody against c-Myc(MYC909), APC conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNCAP0275-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC275), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNCAP0275-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC275), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNCAP0699-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC699), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNCAP0699-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC699), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNCB0909-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC909), Biotin conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNCB0909-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC909), Biotin conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNC880275-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC275), CF488A conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNC880275-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC275), CF488A conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNCR0275-250 250uL
EUR 459.6
Description: Primary antibody against c-Myc(MYC275), RPE conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNCR0699-250 250uL
EUR 459.6
Description: Primary antibody against c-Myc(MYC699), RPE conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNCR0909-250 250uL
EUR 459.6
Description: Primary antibody against c-Myc(MYC909), RPE conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNCP0275-250 250uL
EUR 459.6
Description: Primary antibody against c-Myc(MYC275), PerCP conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNCP0699-250 250uL
EUR 459.6
Description: Primary antibody against c-Myc(MYC699), PerCP conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNCP0909-250 250uL
EUR 459.6
Description: Primary antibody against c-Myc(MYC909), PerCP conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNUM0275-50 50uL
EUR 474
Description: Primary antibody against c-Myc(MYC275), 1mg/mL

c-Myc(MYC699) Antibody

BNUM0699-50 50uL
EUR 474
Description: Primary antibody against c-Myc(MYC699), 1mg/mL

c-Myc(MYC909) Antibody

BNUM0909-50 50uL
EUR 474
Description: Primary antibody against c-Myc(MYC909), 1mg/mL

c-Myc(MYC909) Antibody

BNCH0909-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC909), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNCH0909-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC909), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNUB0909-100 100uL
EUR 250.8
Description: Primary antibody against c-Myc(MYC909), Concentration: 0.2mg/mL

c-Myc(MYC909) Antibody

BNUB0909-500 500uL
EUR 549.6
Description: Primary antibody against c-Myc(MYC909), Concentration: 0.2mg/mL

c-Myc(MYC275) Antibody

BNCH0275-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC275), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNCH0275-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC275), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNCH0699-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC699), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNCH0699-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC699), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNUB0275-100 100uL
EUR 250.8
Description: Primary antibody against c-Myc(MYC275), Concentration: 0.2mg/mL

c-Myc(MYC275) Antibody

BNUB0275-500 500uL
EUR 549.6
Description: Primary antibody against c-Myc(MYC275), Concentration: 0.2mg/mL

c-Myc(MYC699) Antibody

BNUB0699-100 100uL
EUR 250.8
Description: Primary antibody against c-Myc(MYC699), Concentration: 0.2mg/mL

c-Myc(MYC699) Antibody

BNUB0699-500 500uL
EUR 549.6
Description: Primary antibody against c-Myc(MYC699), Concentration: 0.2mg/mL

c-Myc(MYC275) Antibody

BNC040275-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC275), CF405S conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNC040275-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC275), CF405S conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNC550275-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC275), CF555 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNC550275-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC275), CF555 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNC550699-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC699), CF555 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNC550699-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC699), CF555 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNC610275-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC275), CF660R conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNC610275-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC275), CF660R conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNC610699-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC699), CF660R conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNC610699-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC699), CF660R conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNC610909-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC909), CF660R conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNC610909-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC909), CF660R conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNC680275-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC275), CF568 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNC680275-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC275), CF568 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNC470275-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC275), CF647 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC275) Antibody

BNC470275-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC275), CF647 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNC470699-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC699), CF647 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNC470699-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC699), CF647 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNC550909-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC909), CF555 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNC550909-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC909), CF555 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNC430699-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC699), CF543 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNC430699-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC699), CF543 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNC430909-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC909), CF543 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNC430909-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC909), CF543 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNC680699-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC699), CF568 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC699) Antibody

BNC680699-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC699), CF568 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNC680909-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC909), CF568 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNC680909-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC909), CF568 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNC470909-100 100uL
EUR 238.8
Description: Primary antibody against c-Myc(MYC909), CF647 conjugate, Concentration: 0.1mg/mL

c-Myc(MYC909) Antibody

BNC470909-500 500uL
EUR 652.8
Description: Primary antibody against c-Myc(MYC909), CF647 conjugate, Concentration: 0.1mg/mL

Polyclonal MYC / c-Myc Antibody (aa25-74)

APR02915G 0.05ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human MYC / c-Myc (aa25-74). This antibody is tested and proven to work in the following applications:

Monoclonal MYC / c-Myc Antibody (aa408-420)

AMM02198G 0.05mg
EUR 580.8
Description: A Monoclonal antibody against Human MYC / c-Myc (aa408-420). The antibodies are raised in Mouse. This antibody is applicable in IHC-P, E

Polyclonal MYC / c-Myc Antibody (aa386-435)

APR02947G 0.05ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human MYC / c-Myc (aa386-435). This antibody is tested and proven to work in the following applications:

Myc Proto-Oncogene Protein (c-Myc) Antibody

20-abx137079
  • EUR 427.20
  • EUR 627.60
  • 0.5 mg
  • 1 mg

Myc Proto-Oncogene Protein (c-MYC) Antibody

abx231791-100ug 100 ug
EUR 661.2

Myc Proto-Oncogene Protein (c-Myc) Antibody

abx139766-01mg 0.1 mg
EUR 460.8

Myc Proto-Oncogene Protein (c-Myc) Antibody

20-abx133153
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Myc Proto-Oncogene Protein (c-Myc) Antibody

20-abx133248
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Myc Proto-Oncogene Protein (c-Myc) Antibody

20-abx133250
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Myc Proto-Oncogene Protein (c-Myc) Antibody

20-abx133912
  • EUR 427.20
  • EUR 644.40
  • EUR 260.40
  • 100 ul
  • 200 ul
  • 30 ul

Myc Proto-Oncogene Protein (c-Myc) Antibody

20-abx118000
  • EUR 661.20
  • EUR 577.20
  • 100 ul
  • 50 ul

Myc Proto-Oncogene Protein (c-Myc) Antibody

abx448567-100ug 100 ug
EUR 627.6

Myc Proto-Oncogene Protein (c-Myc) Antibody

abx448568-100ug 100 ug
EUR 627.6

Myc Proto-Oncogene Protein (c-Myc) Antibody

abx022462-02mg 0.2 mg
EUR 627.6

Myc Proto-Oncogene (c-Myc Peptide) Antibody

abx022463-02mg 0.2 mg
EUR 610.8

Myc Proto-Oncogene (C-Myc Peptide) Antibody

abx023934-100ug 100 ug
EUR 376.8

Myc Proto-Oncogene (C-Myc Peptide) Antibody

abx023935-01ml 0.1 ml
EUR 376.8

Myc Proto-Oncogene Protein (c-Myc) Antibody

abx018354-100ug 100 ug
EUR 594

Myc Proto-Oncogene Protein (c-Myc) Antibody

abx037911-100ug 100 ug
EUR 469.2

Myc Proto-Oncogene Protein (c-Myc) Antibody

abx413504-01mg 0.1 mg
EUR 760.8

Myc Proto-Oncogene Protein (c-Myc) Antibody

abx413821-1mg 1 mg
EUR 994.8

Myc Proto-Oncogene Protein (c-Myc) Antibody

abx413825-2mg 2 mg
EUR 1830

Myc Proto-Oncogene Protein (c-Myc) Antibody

abx413826-01mg 0.1 mg
EUR 493.2

Myc Proto-Oncogene Protein (c-Myc) Antibody

abx413829-20ug 20 ug
EUR 326.4

Myc Proto-Oncogene Protein (c-Myc) Antibody

20-abx008959
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Myc Proto-Oncogene Protein (c-Myc) Antibody

20-abx008960
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Myc Antibody

abx242911-100ul 100 ul
EUR 477.6

Myc antibody

10R-10451 100 ug
EUR 522
Description: Mouse monoclonal Myc antibody

MYC antibody

10R-10606 100 ug
EUR 418.8
Description: Mouse monoclonal MYC antibody

MYC antibody

10R-1757 100 ug
EUR 614.4
Description: Mouse monoclonal MYC antibody

Myc antibody

10R-4914 100 ul
EUR 871.2
Description: Mouse monoclonal Myc antibody

Myc antibody

10R-4915 100 ul
EUR 829.2
Description: Mouse monoclonal Myc antibody

Myc antibody

10R-4916 100 ul
EUR 829.2
Description: Mouse monoclonal Myc antibody

Myc antibody

10R-7246 50 ug
EUR 337.2
Description: Mouse monoclonal Myc antibody

Myc antibody

20R-1901 50 ug
EUR 337.2
Description: Rabbit polyclonal Myc antibody

Myc antibody

20R-1902 50 ug
EUR 337.2
Description: Rabbit polyclonal Myc antibody

Myc antibody

20R-1903 50 ug
EUR 337.2
Description: Rabbit polyclonal Myc antibody

Myc antibody

20R-2131 50 ug
EUR 337.2
Description: Rabbit polyclonal Myc antibody

Myc antibody

20R-2218 50 ug
EUR 337.2
Description: Rabbit polyclonal Myc antibody

Myc antibody

20R-2219 50 ug
EUR 337.2
Description: Rabbit polyclonal Myc antibody

The outcomes confirmed that a number of transgene fragments have been detected till 11 days. These findings point out that the mix of plasma cfDNA or only one drop of complete blood with TaqMan-qPCR assay is possible to detect plasmid-PEI-based gene doping. Our findings may speed up the event of strategies for detecting gene doping in people.

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