Production of lentiviral vectors in suspension cells using low proportion of supercoiled circular plasmid DNA

Production of lentiviral vectors in suspension cells using low proportion of supercoiled circular plasmid DNA

The supercoiled round (SC) topology type of plasmid DNA has been regarded to be advantageous over open round or linearized analogue in transfection and expression effectivity, and due to this fact are largely demanded within the biopharmaceutical manufacturing. Nevertheless, manufacturing of high-purity SC plasmid DNA would lead to excessive manufacturing value. The impact of SC proportion in plasmid DNA on the standard of packaged lentiviral vectors has by no means been reported. On this research, we established an environment friendly system for manufacturing of high-titer lentiviral vectors utilizing suspension HEK293SF cells in serum-free media, and the lentiviral titer was not related to the proportion of SC plasmid DNA.

Plasmids DNA with totally different proportion of SC, open-circular, and linearized kinds have been ready utilizing the thermal denaturation technique, and have been transfected to adherent HEK293T or suspension HEK293SF cells for packaging of lentiviral vectors. The titer of lentiviral vectors from HEK293T cells, however not from HEK293SF cells, was considerably impaired when the proportion of SC plasmid DNA decreased from 60-80% to 30-40%. Additional lower of SC plasmid proportion to three% led to a dramatic discount of lentiviral titer irrespective of the packaging cell line was.

Nevertheless, lentiviral vectors from HEK293SF cells nonetheless confirmed a excessive titer even when the proportion of SC plasmid DNA was 3%. This research demonstrated that extraordinarily excessive proportion of SC plasmid DNA was not required for packaging of high-titer lentiviral vector in HEK293SF cells, a minimum of underneath our manufacturing course of.

Improvement of a Plasmid Shuttle Vector System for Genetic Manipulation of Chlamydia psittaci

The obligate intracellular bacterium Chlamydia psittaci is a identified avian pathogen inflicting psittacosis in birds and is able to zoonotic transmission. In human pulmonary infections, C. psittaci could cause pneumonia related to vital mortality if inadequately identified and handled. Though intracellular C. psittaci manipulates host cell organelles for its replication and survival, it has been troublesome to display host-pathogen interactions in C. psittaci an infection because of the lack of easy-to-handle genetic manipulation instruments. Right here, we present the genetic transformation of C. psittaci utilizing a plasmid shuttle vector that incorporates a controllable gene induction system.

The 7,553-bp plasmid p01DC12 was ready from the nonavian C. psittaci pressure 01DC12. We constructed the shuttle vector pCps-Tet-mCherry utilizing the complete sequence of p01DC12 and the 4,449-bp fragment of Chlamydia trachomatis shuttle vector pBOMB4-Tet-mCherry. pCps-Tet-mCherry contains genes encoding the inexperienced fluorescent protein (GFP), mCherry, and ampicillin resistance (AmpR). Goal genes will be inserted at a a number of cloning website (MCS). Importantly, these genes will be regulated by a tetracycline-inducible (tet) promoter.

Utilizing the pCps-Tet-mCherry plasmid shuttle vector, we present the expression of GFP, in addition to the induction of mCherry expression, in C. psittaci pressure 02DC15, which belongs to the avian C. psittaci 6BC clade. Moreover, we demonstrated that pCps-Tet-mCherry was stably retained in C. psittaci transformants. Thus, our C. psittaci plasmid shuttle vector system represents a novel focused method that allows the elucidation of host-pathogen interactions.

Psittacosis, brought on by avian C. psittaci, has a serious financial impression within the poultry business worldwide and represents a big danger for zoonotic transmission to people. Prior to now decade, the instruments of genetic manipulation have been improved for chlamydial molecular research. Whereas a number of genetic instruments have been primarily developed in Chlamydia trachomatis, a secure gene-inducible shuttle vector system has to not date been accessible for C. psittaci On this research, we tailored a C. trachomatis plasmid shuttle vector system to C. psittaci

Production of lentiviral vectors in suspension cells using low proportion of supercoiled circular plasmid DNA

We constructed a C. psittaci plasmid spine shuttle vector referred to as pCps-Tet-mCherry. The assemble expresses GFP in C. psittaci Importantly, exogeneous genes will be inserted at an MCS and are regulated by a tet promoter. The appliance of the pCps-Tet-mCherry shuttle vector system permits a promising new method to research unknown gene features of this pathogen.

Detection of A number of Transgene Fragments in a Mouse Mannequin of Gene Doping Primarily based on Plasmid Vector Utilizing TaqMan-qPCR Assay

The World Anti-Doping Company has prohibited gene doping within the context of progress in gene remedy. There’s a danger that the augmentation of genes utilizing plasmids may very well be utilized for gene doping. Nevertheless, no gold commonplace technique to detect this has been established. Right here, we aimed to develop a way to detect a number of transgene fragments as proof of gene doping. Firstly, gene supply mannequin mice as a mimic of gene doping have been created by injecting firefly luciferase plasmid with polyethylenimine (PEI) into the belly cavity.

The outcomes confirmed profitable institution of the mannequin, with ample luminescence upon in vivo imaging. Subsequent, a number of transgene fragments within the mannequin have been detected in plasma cell-free (cf)DNA, blood-cell-fraction DNA, and stool DNA utilizing the TaqMan- quantitative real-time PCR(qPCR) assay, with the very best ranges in plasma cfDNA. Utilizing only a single drop of complete blood from the mannequin, we additionally tried long-term detection.

Human Glutamyl aminopeptidase (ENPEP)

1-CSB-RP147594h(c)
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Glutamyl aminopeptidase(ENPEP),partial expressed in E.coli

Saccharomyces cerevisiae Trehalose-phosphatase (TPS2)

1-CSB-RP165694Ye(c)
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Saccharomyces cerevisiae Trehalose-phosphatase(TPS2),partial expressed in E.coli

Mouse Protein Muc5ac (Muc5ac)

1-CSB-RP172294m(C)
  • EUR 606.00
  • EUR 318.00
  • EUR 2192.40
  • EUR 919.20
  • EUR 1461.60
  • EUR 402.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Mouse Protein Muc5ac(Muc5ac),partial expressed in E.coli

Human Histone deacetylase 7 (HDAC7)

1-CSB-RP178994h(C)
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Histone deacetylase 7(HDAC7),partial expressed in E.coli

Human Telomerase protein component 1 (TEP1)

1-CSB-RP180494h(c)
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Telomerase protein component 1(TEP1),partial expressed in E.coli

Human Collagen alpha-1 (XVII) chain (COL17A1)

1-CSB-RP180994h(c)
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Collagen alpha-1(XVII) chain(COL17A1),partial expressed in E.coli

Saccharomyces cerevisiae Neutral trehalase (NTH1)

1-CSB-RP181594Ye(c)
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Saccharomyces cerevisiae Neutral trehalase(NTH1),partial expressed in E.coli

Human V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

DLR-MYC-Hu-48T 48T
EUR 597.6
Description: A sandwich quantitative ELISA assay kit for detection of Human V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) in samples from tissue homogenates, cell lysates or other biological fluids.

Human V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

DLR-MYC-Hu-96T 96T
EUR 776.4
Description: A sandwich quantitative ELISA assay kit for detection of Human V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) in samples from tissue homogenates, cell lysates or other biological fluids.

Mouse V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

DLR-MYC-Mu-48T 48T
EUR 609.6
Description: A sandwich quantitative ELISA assay kit for detection of Mouse V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) in samples from tissue homogenates, cell lysates or other biological fluids.

Mouse V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

DLR-MYC-Mu-96T 96T
EUR 793.2
Description: A sandwich quantitative ELISA assay kit for detection of Mouse V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) in samples from tissue homogenates, cell lysates or other biological fluids.

Rat V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

DLR-MYC-Ra-48T 48T
EUR 633.6
Description: A sandwich quantitative ELISA assay kit for detection of Rat V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids.

Rat V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

DLR-MYC-Ra-96T 96T
EUR 828
Description: A sandwich quantitative ELISA assay kit for detection of Rat V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids.

Human V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

RD-MYC-Hu-48Tests 48 Tests
EUR 600

Human V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

RD-MYC-Hu-96Tests 96 Tests
EUR 830.4

Mouse V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

RD-MYC-Mu-48Tests 48 Tests
EUR 613.2

Mouse V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

RD-MYC-Mu-96Tests 96 Tests
EUR 850.8

Rat V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

RD-MYC-Ra-48Tests 48 Tests
EUR 640.8

Rat V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

RD-MYC-Ra-96Tests 96 Tests
EUR 890.4

Human V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

RDR-MYC-Hu-48Tests 48 Tests
EUR 626.4

Human V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

RDR-MYC-Hu-96Tests 96 Tests
EUR 868.8

Mouse V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

RDR-MYC-Mu-48Tests 48 Tests
EUR 640.8

Mouse V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

RDR-MYC-Mu-96Tests 96 Tests
EUR 890.4

Rat V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

RDR-MYC-Ra-48Tests 48 Tests
EUR 669.6

Rat V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

RDR-MYC-Ra-96Tests 96 Tests
EUR 931.2

c-Myc Epitope (Myc) Antibody

20-abx132269
  • EUR 526.80
  • EUR 159.60
  • EUR 1513.20
  • EUR 727.20
  • EUR 410.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

c-Myc Epitope (Myc) Antibody

20-abx130438
  • EUR 376.80
  • EUR 910.80
  • EUR 493.20
  • EUR 184.80
  • EUR 292.80
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

c-Myc Oncoprotein; Clone 9E10.3 (Concentrate)

RA0226-C.1 0.1 ml
EUR 150

c-Myc Oncoprotein; Clone 9E10.3 (Concentrate)

RA0226-C.5 0.5 ml
EUR 360

c-Myc Oncoprotein; Clone MYC909 (Concentrate)

RA0227-C.1 0.1 ml
EUR 150

c-Myc Oncoprotein; Clone MYC909 (Concentrate)

RA0227-C.5 0.5 ml
EUR 360

C-myc antibody

70R-10651 1 ml
EUR 516
Description: Affinity purified Chicken polyclonal C-myc antibody

C-myc antibody

70-CC18 100 ug
EUR 397.2
Description: Affinity purified Chicken polyclonal C-myc antibody

c Myc antibody

10R-8403 100 ul
EUR 471.6
Description: Mouse monoclonal c Myc antibody

c-Myc antibody

70R-32862 100 ug
EUR 548.4
Description: Rabbit polyclonal c-Myc antibody

C-myc Antibody

48044-100ul 100ul
EUR 399.6

C-myc Antibody

48044-50ul 50ul
EUR 286.8

C-myc antibody

20R-CC001 200 ug
EUR 457.2
Description: Chicken polyclonal C-myc antibody

C-myc antibody

10R-C101a 100 ug
EUR 651.6
Description: Mouse monoclonal C-myc antibody

C-myc antibody

70R-CG001 100 ul
EUR 457.2
Description: Affinity purified Goat polyclonal C-myc antibody

c-Myc Antibody

AF7674 200ul
EUR 540

c-Myc Antibody

AF6054 200ul
EUR 420

c-Myc Antibody

AF6055 200ul
EUR 420

c-Myc Antibody

AF0358 200ul
EUR 420

c-Myc Antibody

R30632 100 ug
EUR 419

c-Myc Antibody

RQ4860 100ul
EUR 419

c-Myc Antibody

RQ4861 100ul
EUR 419

c-Myc Antibody

F52056-0.08ML 0.08 ml
EUR 165

c-Myc Antibody

F52056-0.4ML 0.4 ml
EUR 389

c-Myc Antibody

F47610-0.08ML 0.08 ml
EUR 165

c-Myc Antibody

F47610-0.4ML 0.4 ml
EUR 379

C-Myc Antibody

3995-100 each
EUR 392.4

C-Myc Antibody

3995-30T each
EUR 175.2

C-Myc Antibody

6767-100 each
EUR 379.2

c-Myc Antibody

V8412-100UG 100 ug
EUR 499
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V8412-20UG 20 ug
EUR 219
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V8412SAF-100UG 100 ug
EUR 499
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V8454-100UG 100 ug
EUR 499
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V8454-20UG 20 ug
EUR 219
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V8454SAF-100UG 100 ug
EUR 499
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V2744-100UG 100 ug
EUR 499
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This mAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V2744-20UG 20 ug
EUR 219
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This mAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V2744IHC-7ML 7 ml
EUR 499
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This mAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V2744SAF-100UG 100 ug
EUR 499
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This mAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V2745-100UG 100 ug
EUR 499
Description: Myc, or c-Myc, is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-Myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V2745-20UG 20 ug
EUR 219
Description: Myc, or c-Myc, is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-Myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V2745IHC-7ML 7 ml
EUR 499
Description: Myc, or c-Myc, is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-Myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V2745SAF-100UG 100 ug
EUR 499
Description: Myc, or c-Myc, is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-Myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V2746-100UG 100 ug
EUR 499
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. This mAb shows no cross-reaction with v-myc. c-Myc is a transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. [UniProt]

c-Myc Antibody

V2746-20UG 20 ug
EUR 219
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. This mAb shows no cross-reaction with v-myc. c-Myc is a transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. [UniProt]

c-Myc Antibody

V2746IHC-7ML 7 ml
EUR 499
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. This mAb shows no cross-reaction with v-myc. c-Myc is a transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. [UniProt]

c-Myc Antibody

V2746SAF-100UG 100 ug
EUR 499
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. This mAb shows no cross-reaction with v-myc. c-Myc is a transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. [UniProt]

c-Myc Antibody

V3124-100UG 100 ug
EUR 499
Description: The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

c-Myc Antibody

V3124-20UG 20 ug
EUR 219
Description: The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

c-Myc Antibody

V3124SAF-100UG 100 ug
EUR 499
Description: The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

c-Myc Antibody

V3125-100UG 100 ug
EUR 499
Description: The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

c-Myc Antibody

V3125-20UG 20 ug
EUR 219
Description: The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

c-Myc Antibody

V3125SAF-100UG 100 ug
EUR 499
Description: The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

c-Myc Antibody

V3158-100UG 100 ug
EUR 499
Description: The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

c-Myc Antibody

V3158-20UG 20 ug
EUR 219
Description: The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

c-Myc Antibody

V3158IHC-7ML 7 ml
EUR 499
Description: The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

c-Myc Antibody

V3158SAF-100UG 100 ug
EUR 499
Description: The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

c-Myc Antibody

V7021-100UG 100 ug
EUR 499
Description: Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. [UniProt]

c-Myc Antibody

V7021-20UG 20 ug
EUR 219
Description: Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. [UniProt]

c-Myc Antibody

V7021IHC-7ML 7 ml
EUR 499
Description: Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. [UniProt]

c-Myc Antibody

V7021SAF-100UG 100 ug
EUR 499
Description: Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. [UniProt]

c-Myc Antibody

RQ6663 100ug
EUR 419
Description: MYC proto-oncogene, bHLH transcription factor is a protein that in humans is encoded by the MYC gene which is a member of the myc family of transcription factors. The protein contains basic helix-loop-helix (bHLH) structural motif. This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.

c-Myc Antibody

V7971-100UG 100 ug
EUR 499
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V7971-20UG 20 ug
EUR 219
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

c-Myc Antibody

V7971SAF-100UG 100 ug
EUR 499
Description: It recognizes a transcription factor of 64-67kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

Myc Proto-Oncogene Protein (c-Myc) Antibody

20-abx137079
  • EUR 427.20
  • EUR 627.60
  • 0.5 mg
  • 1 mg

Myc Proto-Oncogene Protein (c-Myc) Antibody

abx139766-01mg 0.1 mg
EUR 460.8

Myc Proto-Oncogene Protein (c-Myc) Antibody

20-abx133153
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Myc Proto-Oncogene Protein (c-Myc) Antibody

20-abx133248
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Myc Proto-Oncogene Protein (c-Myc) Antibody

20-abx133250
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Myc Proto-Oncogene Protein (c-Myc) Antibody

20-abx133912
  • EUR 427.20
  • EUR 644.40
  • EUR 260.40
  • 100 ul
  • 200 ul
  • 30 ul

Myc Proto-Oncogene Protein (c-Myc) Antibody

abx037911-100ug 100 ug
EUR 469.2

Myc Proto-Oncogene Protein (c-Myc) Antibody

20-abx008959
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Myc Proto-Oncogene Protein (c-Myc) Antibody

20-abx008960
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Myc Proto-Oncogene Protein (c-Myc) Antibody

20-abx118000
  • EUR 661.20
  • EUR 577.20
  • 100 ul
  • 50 ul

Myc Proto-Oncogene Protein (c-Myc) Antibody

abx018354-100ug 100 ug
EUR 594

Myc Proto-Oncogene Protein (c-Myc) Antibody

abx022462-02mg 0.2 mg
EUR 627.6

Myc Proto-Oncogene (c-Myc Peptide) Antibody

abx022463-02mg 0.2 mg
EUR 610.8

Myc Proto-Oncogene (C-Myc Peptide) Antibody

abx023934-100ug 100 ug
EUR 376.8

Myc Proto-Oncogene (C-Myc Peptide) Antibody

abx023935-01ml 0.1 ml
EUR 376.8

Myc Proto-Oncogene Protein (c-Myc) Antibody

abx413504-01mg 0.1 mg
EUR 760.8

Myc Proto-Oncogene Protein (c-Myc) Antibody

abx413821-1mg 1 mg
EUR 994.8

Myc Proto-Oncogene Protein (c-Myc) Antibody

abx413825-2mg 2 mg
EUR 1830

Myc Proto-Oncogene Protein (c-Myc) Antibody

abx413826-01mg 0.1 mg
EUR 493.2

Myc Proto-Oncogene Protein (c-Myc) Antibody

abx413829-20ug 20 ug
EUR 326.4

Myc Proto-Oncogene Protein (c-MYC) Antibody

abx231791-100ug 100 ug
EUR 661.2

Myc Proto-Oncogene Protein (c-Myc) Antibody

abx448567-100ug 100 ug
EUR 627.6

Myc Proto-Oncogene Protein (c-Myc) Antibody

abx448568-100ug 100 ug
EUR 627.6

Monoclonal MYC / c-Myc Antibody (aa408-420)

AMM02198G 0.05mg
EUR 580.8
Description: A Monoclonal antibody against Human MYC / c-Myc (aa408-420). The antibodies are raised in Mouse. This antibody is applicable in IHC-P, E

Polyclonal MYC / c-Myc Antibody (aa25-74)

APR02915G 0.05ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human MYC / c-Myc (aa25-74). This antibody is tested and proven to work in the following applications:

Polyclonal MYC / c-Myc Antibody (aa386-435)

APR02947G 0.05ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human MYC / c-Myc (aa386-435). This antibody is tested and proven to work in the following applications:

Human recombinant purified His-tag c-myc protein (~65 kda) control

MYC17-C 100 ul
EUR 343.2

C-Myc

MYCF-100T 100 test
EUR 606

c-Myc

RA25085 100 ul
EUR 615.6

C-myc antibody (FITC)

60R-CG001FT 100 ug
EUR 586.8
Description: Goat polyclonal C-myc antibody (FITC) conjugated

C-myc antibody (HRP)

60R-CG001hrp 100 ug
EUR 573.6
Description: Goat polyclonal C-myc antibody (HRP) conjugated

C-myc antibody (FITC)

60R-1085 1 ml
EUR 476.4
Description: Affinity purified Chicken polyclonal C-myc antibody (FITC)

C-myc HRP Antibody

48046-100ul 100ul
EUR 399.6

C-myc HRP Antibody

48046-50ul 50ul
EUR 286.8

c-Myc Polyclonal Antibody

40764-100ul 100ul
EUR 302.4

c-Myc Polyclonal Antibody

40764-50ul 50ul
EUR 224.4

c-Myc Polyclonal Antibody

40765-100ul 100ul
EUR 302.4

c-Myc Polyclonal Antibody

40765-50ul 50ul
EUR 224.4

c-Myc Polyclonal Antibody

40766-100ul 100ul
EUR 302.4

c-Myc Polyclonal Antibody

40766-50ul 50ul
EUR 224.4

c-Myc Tag Antibody

35536-100ul 100ul
EUR 302.4

c-Myc Polyclonal Antibody

ABP55308-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of c-Myc from Human, Mouse, Rat. This c-Myc antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human c-Myc around the non-phosphorylation site of T58

c-Myc Polyclonal Antibody

ABP55308-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of c-Myc from Human, Mouse, Rat. This c-Myc antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human c-Myc around the non-phosphorylation site of T58

c-Myc Polyclonal Antibody

ABP55308-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of c-Myc from Human, Mouse, Rat. This c-Myc antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human c-Myc around the non-phosphorylation site of T58

c-Myc Polyclonal Antibody

ABP55309-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of c-Myc from Human, Mouse, Rat. This c-Myc antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human c-Myc around the non-phosphorylation site of S373

c-Myc Polyclonal Antibody

ABP55309-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of c-Myc from Human, Mouse, Rat. This c-Myc antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human c-Myc around the non-phosphorylation site of S373

c-Myc Polyclonal Antibody

ABP55309-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of c-Myc from Human, Mouse, Rat. This c-Myc antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human c-Myc around the non-phosphorylation site of S373

c-Myc Antibody (HRP)

abx139767-01mg 0.1 mg
EUR 693.6

c-Myc Antibody (Biotin)

abx139768-01mg 0.1 mg
EUR 577.2

c-Myc Antibody (FITC)

abx139769-01mg 0.1 mg
EUR 577.2

c-Myc (pT358) Antibody

20-abx133249
  • EUR 376.80
  • EUR 560.40
  • EUR 243.60
  • 100 ul
  • 200 ul
  • 30 ul

c-Myc Polyclonal Antibody

ABP50183-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of c-Myc from Human. This c-Myc antibody is for WB. It is affinity-purified from rabbit antiserum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein

c-Myc Polyclonal Antibody

ABP50183-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of c-Myc from Human. This c-Myc antibody is for WB. It is affinity-purified from rabbit antiserum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein

c-Myc Polyclonal Antibody

ABP50183-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of c-Myc from Human. This c-Myc antibody is for WB. It is affinity-purified from rabbit antiserum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein

c-Myc (pT58) Antibody

20-abx009775
  • EUR 376.80
  • EUR 560.40
  • EUR 243.60
  • 100 ul
  • 200 ul
  • 30 ul

c-Myc (pS62) Antibody

20-abx009776
  • EUR 376.80
  • EUR 560.40
  • EUR 243.60
  • 100 ul
  • 200 ul
  • 30 ul

c-Myc Polyclonal Antibody

ABP51010-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of c-Myc from Human, Mouse, Rat. This c-Myc antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human c-Myc around the non-phosphorylation site of T358

c-Myc Polyclonal Antibody

ABP51010-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of c-Myc from Human, Mouse, Rat. This c-Myc antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human c-Myc around the non-phosphorylation site of T358

c-Myc Polyclonal Antibody

ABP51010-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of c-Myc from Human, Mouse, Rat. This c-Myc antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human c-Myc around the non-phosphorylation site of T358

The outcomes confirmed that a number of transgene fragments have been detected till 11 days. These findings point out that the mix of plasma cfDNA or only one drop of complete blood with TaqMan-qPCR assay is possible to detect plasmid-PEI-based gene doping. Our findings may speed up the event of strategies for detecting gene doping in people.

Leave a Reply

Your email address will not be published. Required fields are marked *