The US Meals and Drug Administration (FDA) regulates manufacturing and testing of superior therapeutic medicinal merchandise (ATMPs) to make sure the security of every product for human use. Gold normal sterility testing (USP<71>) and different blood tradition methods have main limitations for the detection of fungal contaminants. On this research, we evaluated the efficiency of iLYM media (designed initially for the meals and beverage trade) to evaluate its potential to be used within the biopharmaceutical subject for ATMP sterility testing.
We performed a parallel analysis of 4 totally different take a look at methods (USP<71>, BacT/ALERT, BACTEC, and Sabouraud Dextrose Agar [SDA] tradition), three totally different bottle media formulations (iLYM, iFA+, and Myco/F Lytic), and two incubation temperatures (22.5°C and 32.5-35°C) utilizing a various set of fungi (n=51) remoted from NIH cleanroom environments and former product contaminants. Moreover, we evaluated the impact of agitation versus “delayed entry” static pre-incubation on take a look at sensitivity and time to detection (TTD). General, delayed entry of bottles onto the BacT/ALERT or BACTEC devices (with agitation) didn’t enhance take a look at efficiency.
USP<71> and SDA tradition continued to considerably outperform every automated tradition situation alone. Nonetheless, we present for the primary time, {that a} closed-system, dual-bottle mixture of iLYM 22.5°C and iFA+ 32.5°C can present excessive fungal sensitivity, statistically corresponding to USP<71>, when examined in opposition to a various vary of environmental fungi. Our research fills a much-needed hole in biopharmaceutical testing and provides a good testing algorithm that maximizes bacterial and fungal take a look at sensitivity while minimizing threat of product contamination related to laboratory dealing with.
Gene-Edited Interleukin CAR-T Cells Remedy within the Remedy of Malignancies: Current and Future
Lately, chimeric antigen receptor T cells (CAR-T cells) have been confronted with the issues of weak proliferation and poor persistence within the remedy of some malignancies. Researchers have been attempting to excellent the perform of CAR-T by genetically modifying its construction. Along with the participation of T cell receptor (TCR) and costimulatory alerts, immune cytokines additionally exert a decisive function within the activation and proliferation of T cells.
Due to this fact, genetic engineering methods had been used to generate cytokines to boost tumor killing perform of CAR-T cells. When CAR-T cells are in touch with goal tumor tissue, the proliferation means and persistence of T cells might be improved by structurally or inductively releasing immunoregulatory molecules to the tumor area.
There are numerous CAR-T cells research on gene-edited cytokines, and the most typical cytokines concerned are interleukins (IL-7, IL-12, IL-15, IL-18, IL-21, IL-23). Strategies for the development of gene-edited interleukin CAR-T cells embrace co-expression of single interleukin, two interleukin, interleukin mixed with different cytokines, interleukin receptors, interleukin subunits, and fusion inverted cytokine receptors (ICR).
Preclinical and medical trials have yielded constructive outcomes, and lots of extra are underneath manner. By studying numerous literatures, we summarized the purposeful traits of some members of the interleukin household associated to tumor immunotherapy, and described the analysis standing of gene-edited interleukin CAR-T cells within the remedy of malignant tumors. The target is to discover the optimized technique of gene edited interleukin-CAR-T cell perform.
Improved Supply Strategies for Gene Remedy and Cell Transplantation in Parkinson’s Illness
Quite a lot of cell transplantation and gene remedy trials have been carried out during the last three many years in an effort to revive perform in Parkinson’s illness. A lot has been realized about optimizing supply strategies for these therapeutics. That is significantly true in gene remedy, which has predominated the medical trial panorama in recent times; nonetheless, cell transplantation for Parkinson’s illness is at present present process a renaissance.
Improvements reminiscent of cannula design, iMRI-guided surgical procedure and an evolution in supply technique has radically modified the way in which investigators method medical trial design. Future therapeutic methods could make use of newer supply strategies reminiscent of chronically implanted infusion gadgets and focal opening of the blood mind barrier with centered ultrasound.
SLC4A11 and MFSD3 Gene Expression Adjustments in Deoxynivalenol Handled IPEC-J2 Cells
Deoxynivalenol (DON) brought about critical cytotoxicity for animal cells. Nonetheless, genes concerned in regulating DON toxicity and the underlying molecular mechanisms stay largely unknown. This research explored the function of SLC4A11 and MFSD3 in assuaging DON toxicity and analyzed the DNA methylation adjustments of those two genes.
Viability and cell cycle evaluation confirmed that DON publicity decreased the IPEC-J2 viability (P < 0.01), blocked the cell cycle within the G2/M part (P < 0.01), and elevated the speed of apoptosis (P < 0.05). Expression of the SLC4A11 and MFSD3 genes was considerably downregulated upon DON publicity (P < 0.01).
Overexpression of SLC4A11 and MFSD3 can improve the cell viability (P < 0.01). DNA methylation assays indicated that promoter methylation of SLC4A11 (mC-1 and mC-23) and MFSD3 (mC-1 and mC-12) had been considerably increased in contrast with these within the controls and correlated negatively with mRNA expression (P < 0.05).
Stem Cell MicroRNA Profiling Panel |
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| RA620A-1 | SBI | 10 profiles | EUR 1406.4 |
Mouse Stem Cell Factor (SCF) AssayMax ELISA Kit |
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| EMS1001-1 | AssayPro | 96 Well Plate | EUR 475.2 |
Mouse Nanog Stem Cell Differentiation Reporter (pGreenZeo, plasmid) |
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| SR10031PA-1 | SBI | 10 ug | EUR 2098.8 |
SARS-CoV-2 Spike S1 RBD Protein, Mouse Fc-fusion |
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| E80026 | EpiGentek |
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Mouse Nanog Stem Cell Differentiation Reporter (pGreenZeo, pre-packaged) |
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| SR10031VA-1 | SBI | >2 x 10^6 IFUs | EUR 906 |
Polyclonal Goat anti-GST α-form |
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| GST-ANTI-1 | Detroit R&D | 50 uL | EUR 336 |
Histone H3 Methylation Antibody Panel Pack I – Active Genes |
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| C10000 | EpiGentek |
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Histone H3 Methylation Antibody Panel Pack I – Repression Genes |
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| C10001 | EpiGentek |
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Histone H3 Methylation Antibody Panel Pack II – Active Genes |
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| C10002 | EpiGentek |
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Histone H3 Methylation Antibody Panel Pack II – Repression Genes |
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| C10003 | EpiGentek |
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Histone H3 Methylation Antibody Panel Pack III – Active Genes |
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| C10004 | EpiGentek |
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Histone H3K4 Methylation Antibody Panel Pack |
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| C10005 | EpiGentek |
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Histone H3K9 Methylation Antibody Panel Pack |
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| C10006 | EpiGentek |
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Histone H3K27 Methylation Antibody Panel Pack |
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| C10007 | EpiGentek |
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Histone H3K36 Methylation Antibody Panel Pack |
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| C10008 | EpiGentek |
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Histone H3K79 Methylation Antibody Panel Pack |
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| C10009 | EpiGentek |
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Histone H3 Acetylation Antibody Panel Pack I |
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| C10010 | EpiGentek |
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Histone H3 Acetylation Antibody Panel Pack II |
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| C10011 | EpiGentek |
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Histone H4K20 Methylation Antibody Panel Pack |
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| C10012 | EpiGentek |
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Histone H4 Acetylation Antibody Panel Pack |
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| C10013 | EpiGentek |
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Histone H3 Phosphorylation Antibody Panel Pack |
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| C10014 | EpiGentek |
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Histone H3R2 Methylation Antibody Panel Pack |
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| C10015 | EpiGentek |
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Histone H3R8 Methylation Antibody Panel Pack |
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| C10016 | EpiGentek |
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Histone H3R17 Methylation Antibody Panel Pack |
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| C10017 | EpiGentek |
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Histone H3R26 Methylation Antibody Panel Pack |
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| C10018 | EpiGentek |
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Histone H4R3 Methylation Antibody Panel Pack |
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| C10019 | EpiGentek |
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DNA Methylation Antibody Panel Pack I |
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| C20000 | EpiGentek |
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DNMT3A Protein |
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| E11000 | EpiGentek |
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TET1 Protein (Active) |
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| E12002 | EpiGentek |
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DNMT3B Protein |
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| E14000 | EpiGentek |
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DNMT1 Protein |
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| E15000 | EpiGentek |
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HDAC1 Protein |
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| E24002 | EpiGentek |
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HDAC10 Protein |
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| E24003 | EpiGentek |
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HDAC11 Protein |
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| E24004 | EpiGentek |
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HDAC2 Protein |
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| E24005 | EpiGentek |
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HDAC3 Protein |
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| E24006 | EpiGentek |
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HDAC4 Protein |
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| E24007 | EpiGentek |
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HDAC5 Protein |
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| E24008 | EpiGentek |
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HDAC6 Protein |
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| E24009 | EpiGentek |
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HDAC7 Protein |
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| E24010 | EpiGentek |
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HDAC8 Protein |
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| E24011 | EpiGentek |
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HDAC9 Protein |
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| E24012 | EpiGentek |
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H3F3A Protein |
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| E35003 | EpiGentek |
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H4 Protein |
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| E35005 | EpiGentek |
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SOD1 Protein |
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| E70000 | EpiGentek |
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EpiMag HT (96-Well) Magnetic Separator |
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| Q10002 | EpiGentek |
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EpiMag 96-Well Microplate (5/pack) |
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| Q10003 | EpiGentek |
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Human Genomic DNA |
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| X11000 | EpiGentek |
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Human Brain Genomic DNA |
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| X11001 | EpiGentek |
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SCA-1 Antibody |
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| 47996-100ul | SAB | 100ul | EUR 399.6 |
SCA-1 Antibody |
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| 47996-50ul | SAB | 50ul | EUR 286.8 |
SCF Stem Cell Factor Human Recombinant Protein |
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| PROTP21583-1 | BosterBio | Regular: 10ug | EUR 380.4 |
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Description: Stem Cell Factor Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18409 Dalton. ;The SCF is purified by proprietary chromatographic techniques. |
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StemBoost? Neural Stem Cell Reprogramming Cocktail (100X) |
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| S238-1 | Biovision | EUR 222 | |
Human Prostate stem cell antigen (PSCA) |
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| 1-CSB-YP018840HU | Cusabio |
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Description: Recombinant Human Prostate stem cell antigen(PSCA) expressed in Yeast |
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Human Prostate stem cell antigen (PSCA) |
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| 1-CSB-EP018840HU | Cusabio |
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Description: Recombinant Human Prostate stem cell antigen(PSCA) expressed in E.coli |
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Human Prostate stem cell antigen (PSCA) |
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| 1-CSB-EP018840HUa0 | Cusabio |
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Description: Recombinant Human Prostate stem cell antigen(PSCA) expressed in E.coli |
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Human Stem Cell Factor (SCF) AssayMax ELISA Kit |
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| ES1001-1 | AssayPro | 96 Well Plate | EUR 475.2 |
ACE2, His-Tag Protein |
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| E80019 | EpiGentek |
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SARS-CoV-2 Spike S1 (13-665) Protein, Fc Fusion, Avi-tag |
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| E80020 | EpiGentek |
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SARS-CoV-2 Spike S1 (16-685) Protein, Avi-His-tag |
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| E80021 | EpiGentek |
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SARS-CoV-2 Spike S1 (16-685) Protein, Fc Fusion, Avi-tag |
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| E80022 | EpiGentek |
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SARS-CoV-2 Spike S1 RBD (V367F) Protein, Avi-His-tag |
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| E80023 | EpiGentek |
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SARS-CoV-2 Spike S1 RBD Protein, Avi-His-tag |
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| E80024 | EpiGentek |
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SARS-CoV-2 Spike S1 RBD Protein, Human Fc-Fusion, Avi-Tag |
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| E80025 | EpiGentek |
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SARS-CoV-2 Nucleocapsid Protein, Avi-His-tag |
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| E80027 | EpiGentek |
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Recombinant SARS-CoV-2 Spike Glycoprotein(S) (D614G), Partial |
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| E80028 | EpiGentek |
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SCA-1 Conjugated Antibody |
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| C47996 | SAB | 100ul | EUR 476.4 |
Anti-Periphilin 1 Antibody |
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| A06996-1 | BosterBio | 100ul | EUR 476.4 |
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Description: Rabbit Polyclonal Antibody for Periphilin 1 Antibody (PPHLN1) detection.tested for WB in Human, Mouse. |
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Anti-Lyl-1 Antibody |
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| A07491-1 | BosterBio | 100ul | EUR 476.4 |
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Description: Rabbit Polyclonal Lyl-1 Antibody. Validated in IHC and tested in Human, Mouse, Rat. |
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Anti-GLI-1 Antibody |
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| A07972-1 | BosterBio | 100ul | EUR 476.4 |
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Description: Rabbit Polyclonal Antibody for GLI-1 Antibody (ACSS1) detection. Tested with WB in Human, Mouse, Rat. |
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Anti-Cerebellin 1 Antibody |
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| A09176-1 | BosterBio | 100ul | EUR 476.4 |
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Description: Rabbit Polyclonal Antibody for Cerebellin 1 Antibody (CBLN1) detection. Tested with WB in Human, Mouse, Rat. |
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Anti-DOC-1 Antibody |
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| A09467-1 | BosterBio | 100ul | EUR 476.4 |
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Description: Rabbit Polyclonal Antibody for DOC-1 Antibody (CDK2AP1) detection. Tested with WB in Human, Mouse. |
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Anti-NPDC-1 Antibody |
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| A12846-1 | BosterBio | 100ul | EUR 476.4 |
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Description: Rabbit Polyclonal Antibody for NPDC-1 Antibody (NPDC1) detection. Tested with WB in Human, Mouse, Rat. |
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Anti-ROBO-1 Antibody |
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| A01530-1 | BosterBio | 100ug | EUR 546 |
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Description: Rabbit Polyclonal ROBO-1 Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat. |
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Anti-Bag-1 Antibody |
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| A02423-1 | BosterBio | 50 ul | EUR 476.4 |
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Description: Rabbit Polyclonal Bag-1 Antibody. Validated in IP, IHC and tested in Bovine, Canine, Human, Mouse, Rat. |
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Anti-TUB 1 Antibody |
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| A02917-1 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-Dok-1 Antibody |
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| A03039-1 | BosterBio | 100ul | EUR 476.4 |
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Description: Rabbit Polyclonal Antibody for Dok-1 Antibody (DOK1) detection.tested for WB in Human, Mouse, Rat. |
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Anti-TFIIIB90-1 Antibody |
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| A03761-1 | BosterBio | 100ul | EUR 476.4 |
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Description: Rabbit Polyclonal Antibody for TFIIIB90-1 Antibody (BRF1) detection.tested for WB in Human, Mouse. |
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Anti-Atrophin-1 Antibody |
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| A03828-1 | BosterBio | 100ul | EUR 476.4 |
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Description: Rabbit Polyclonal Antibody for Atrophin-1 Antibody (ATN1) detection. Tested with WB in Human, Mouse, Rat. |
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Anti-CNG-1 Antibody |
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| A05494-1 | BosterBio | 100ul | EUR 476.4 |
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Description: Rabbit Polyclonal Antibody for CNG-1 Antibody (CNGA1) detection. Tested with WB in Human, Mouse, Rat. |
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Anti-PAI-1 Antibody |
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| A00637-1 | BosterBio | 100ul | EUR 476.4 |
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Description: Rabbit Polyclonal Antibody for PAI-1 Antibody (SERPINE1) detection.tested for WB in Human, Mouse, Rat. |
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Anti-Flk-1 Antibody |
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| A00901-1 | BosterBio | 100ul | EUR 476.4 |
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Description: Rabbit Polyclonal Antibody for Flk-1 Antibody (KDR) detection.tested for WB in Human, Mouse. |
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Anti-EDG-1 Antibody |
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| A01502-1 | BosterBio | 100ul | EUR 476.4 |
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Description: Rabbit Polyclonal Antibody for EDG-1 Antibody (S1PR1) detection.tested for WB in Human, Mouse, Rat. |
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Anti-PARP-1 Antibody |
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| A00122-1 | BosterBio | 100ul | EUR 476.4 |
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Description: Rabbit Polyclonal PARP-1 Antibody. Validated in ELISA, IP, IF, WB and tested in Human, Mouse. |
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Anti-Presenilin 1 Antibody |
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| A00138-1 | BosterBio | 100ul | EUR 476.4 |
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Description: Rabbit Polyclonal Antibody for Presenilin 1 Antibody (PSEN1) detection. Tested with WB, IHC in Human, Mouse, Rat. |
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Methylamp 96 DNA Modification Kit |
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| P-1008 | EpiGentek |
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Nucleic Acid Isolation Enhancer (Glycogen Solution) |
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| R-1001 | EpiGentek |
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Methylamp PCR Enhancer |
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| R-1002 | EpiGentek |
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Streptavidin: HRP Conjugate |
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| R-1098 | EpiGentek |
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Streptavidin |
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| R-1100 | EpiGentek |
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Protease Inhibitor Cocktail |
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| R-1101 | EpiGentek |
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Streptavidin-Coated Strip Microwell Plate (Flat) |
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| R-1102 | EpiGentek |
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DNA High Binding Solution |
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| R-1103 | EpiGentek |
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DTT Solution |
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| R-1104 | EpiGentek |
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EpiQuik Nuclear Extraction Kit |
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| OP-0002 | EpiGentek |
|
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Human Prostate stem cell antigen(PSCA) ELISA kit |
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| 1-CSB-EL018840HU | Cusabio |
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Description: Quantitativesandwich ELISA kit for measuring Human Prostate stem cell antigen(PSCA) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
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Hemokinin 1 (mouse) |
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| B5127-1 | ApexBio | 1 mg | EUR 385.2 |
Anti-HO-1 Monoclonal Antibody (HO-1-2) |
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| M00253-1 | BosterBio | 1mg | EUR 476.4 |
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Description: Mouse Monoclonal HO-1 Antibody (HO-1-2). Validated in Flow Cytometry, IHC, WB and tested in Human, Mouse, Rat. |
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CF543 Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
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| 20325-1 | Biotium | 50uL | EUR 178.8 |
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Description: Minimum order quantity: 1 unit of 50uL |
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CF350 Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
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| 20245-1 | Biotium | 50uL | EUR 178.8 |
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Description: Minimum order quantity: 1 unit of 50uL |
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CF488A Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
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| 20246-1 | Biotium | 50uL | EUR 178.8 |
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Description: Minimum order quantity: 1 unit of 50uL |
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CF555 Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
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| 20247-1 | Biotium | 50uL | EUR 178.8 |
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Description: Minimum order quantity: 1 unit of 50uL |
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CF568 Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
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| 20248-1 | Biotium | 50uL | EUR 178.8 |
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Description: Minimum order quantity: 1 unit of 50uL |
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CF594 Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
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| 20249-1 | Biotium | 50uL | EUR 178.8 |
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Description: Minimum order quantity: 1 unit of 50uL |
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CF633 Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
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| 20250-1 | Biotium | 50uL | EUR 178.8 |
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Description: Minimum order quantity: 1 unit of 50uL |
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CF640R Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
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| 20251-1 | Biotium | 50uL | EUR 178.8 |
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Description: Minimum order quantity: 1 unit of 50uL |
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CF647 Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
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| 20252-1 | Biotium | 50uL | EUR 178.8 |
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Description: Minimum order quantity: 1 unit of 50uL |
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CF680 Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
|||
| 20253-1 | Biotium | 50uL | EUR 193.2 |
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Description: Minimum order quantity: 1 unit of 50uL |
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CF770 Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
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| 20254-1 | Biotium | 50uL | EUR 193.2 |
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Description: Minimum order quantity: 1 unit of 50uL |
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Anti-Pyrophosphatase 1/PPA1 Antibody |
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| A07485-1 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-TCP-1 eta Antibody |
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| A08169-1 | BosterBio | 100ul | EUR 476.4 |
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Description: Rabbit Polyclonal Antibody for TCP-1 eta Antibody (CCT7) detection. Tested with WB in Human, Mouse, Rat. |
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Anti-Relaxin 1/RLN1 Antibody |
|||
| A08367-1 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-TCP-1 zeta Antibody |
|||
| A09373-1 | BosterBio | 100ul | EUR 476.4 |
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Description: Rabbit Polyclonal Antibody for TCP-1 zeta Antibody (CCT6A) detection.tested for WB in Human, Mouse, Rat. |
|||
Anti-CHST8/Galnac4St 1 Antibody |
|||
| A10989-1 | BosterBio | 100ul | EUR 476.4 |
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Description: Rabbit Polyclonal CHST8/Galnac4St 1 Antibody. Validated in WB and tested in Human, Mouse, Rat. |
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Anti-Synaptotagmin 1/SYT1 Antibody |
|||
| A02314-1 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-Syntenin-1 Monoclonal Antibody |
|||
| A02475-1 | BosterBio | 100ul | EUR 476.4 |
|
Description: Mouse Monoclonal Antibody for Syntenin-1 Antibody (SDCBP) detection. Tested with WB in Human, Rat, Dog, Pig. |
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Anti-Dsg1/Desmoglein 1 Antibody |
|||
| A02655-1 | BosterBio | 100ul | EUR 476.4 |
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Description: Rabbit Polyclonal Antibody for Dsg1 Antibody (DSG1) detection.tested for IHC, WB in Human, Mouse, Rat. |
|||
Anti-CAF-1 p150 Antibody |
|||
| A02732-1 | BosterBio | 100ul | EUR 476.4 |
|
Description: Rabbit Polyclonal Antibody for CAF-1 p150 Antibody (CHAF1A) detection. Tested with WB in Human, Mouse. |
|||
Anti-Talin 1/TLN1 Antibody |
|||
| A02859-1 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-Islet 1/ISL1 Antibody |
|||
| A02969-1 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-Musashi 1/Msi1 Antibody |
|||
| A05052-1 | BosterBio | 100ug/vial | EUR 400.8 |
Additional evaluation confirmed that mC-1 of SLC4A11 and MFSD3 was situated in transcription issue binding websites for NF-1 and Sp1. Our findings revealed the novel organic capabilities of porcine SLC4A11 and MFSD3 genes in regulating the cytotoxic results induced by DON, and will contribute to the detection of biomarkers and drug targets for predicting and eliminating the potential toxicity of DON.