The US Meals and Drug Administration (FDA) regulates manufacturing and testing of superior therapeutic medicinal merchandise (ATMPs) to make sure the security of every product for human use. Gold normal sterility testing (USP<71>) and different blood tradition methods have main limitations for the detection of fungal contaminants. On this research, we evaluated the efficiency of iLYM media (designed initially for the meals and beverage trade) to evaluate its potential to be used within the biopharmaceutical subject for ATMP sterility testing.
We performed a parallel analysis of 4 totally different take a look at methods (USP<71>, BacT/ALERT, BACTEC, and Sabouraud Dextrose Agar [SDA] tradition), three totally different bottle media formulations (iLYM, iFA+, and Myco/F Lytic), and two incubation temperatures (22.5°C and 32.5-35°C) utilizing a various set of fungi (n=51) remoted from NIH cleanroom environments and former product contaminants. Moreover, we evaluated the impact of agitation versus “delayed entry” static pre-incubation on take a look at sensitivity and time to detection (TTD). General, delayed entry of bottles onto the BacT/ALERT or BACTEC devices (with agitation) didn’t enhance take a look at efficiency.
USP<71> and SDA tradition continued to considerably outperform every automated tradition situation alone. Nonetheless, we present for the primary time, {that a} closed-system, dual-bottle mixture of iLYM 22.5°C and iFA+ 32.5°C can present excessive fungal sensitivity, statistically corresponding to USP<71>, when examined in opposition to a various vary of environmental fungi. Our research fills a much-needed hole in biopharmaceutical testing and provides a good testing algorithm that maximizes bacterial and fungal take a look at sensitivity while minimizing threat of product contamination related to laboratory dealing with.
Gene-Edited Interleukin CAR-T Cells Remedy within the Remedy of Malignancies: Current and Future
Lately, chimeric antigen receptor T cells (CAR-T cells) have been confronted with the issues of weak proliferation and poor persistence within the remedy of some malignancies. Researchers have been attempting to excellent the perform of CAR-T by genetically modifying its construction. Along with the participation of T cell receptor (TCR) and costimulatory alerts, immune cytokines additionally exert a decisive function within the activation and proliferation of T cells.
Due to this fact, genetic engineering methods had been used to generate cytokines to boost tumor killing perform of CAR-T cells. When CAR-T cells are in touch with goal tumor tissue, the proliferation means and persistence of T cells might be improved by structurally or inductively releasing immunoregulatory molecules to the tumor area.
There are numerous CAR-T cells research on gene-edited cytokines, and the most typical cytokines concerned are interleukins (IL-7, IL-12, IL-15, IL-18, IL-21, IL-23). Strategies for the development of gene-edited interleukin CAR-T cells embrace co-expression of single interleukin, two interleukin, interleukin mixed with different cytokines, interleukin receptors, interleukin subunits, and fusion inverted cytokine receptors (ICR).
Preclinical and medical trials have yielded constructive outcomes, and lots of extra are underneath manner. By studying numerous literatures, we summarized the purposeful traits of some members of the interleukin household associated to tumor immunotherapy, and described the analysis standing of gene-edited interleukin CAR-T cells within the remedy of malignant tumors. The target is to discover the optimized technique of gene edited interleukin-CAR-T cell perform.
Improved Supply Strategies for Gene Remedy and Cell Transplantation in Parkinson’s Illness
Quite a lot of cell transplantation and gene remedy trials have been carried out during the last three many years in an effort to revive perform in Parkinson’s illness. A lot has been realized about optimizing supply strategies for these therapeutics. That is significantly true in gene remedy, which has predominated the medical trial panorama in recent times; nonetheless, cell transplantation for Parkinson’s illness is at present present process a renaissance.
Improvements reminiscent of cannula design, iMRI-guided surgical procedure and an evolution in supply technique has radically modified the way in which investigators method medical trial design. Future therapeutic methods could make use of newer supply strategies reminiscent of chronically implanted infusion gadgets and focal opening of the blood mind barrier with centered ultrasound.
SLC4A11 and MFSD3 Gene Expression Adjustments in Deoxynivalenol Handled IPEC-J2 Cells
Deoxynivalenol (DON) brought about critical cytotoxicity for animal cells. Nonetheless, genes concerned in regulating DON toxicity and the underlying molecular mechanisms stay largely unknown. This research explored the function of SLC4A11 and MFSD3 in assuaging DON toxicity and analyzed the DNA methylation adjustments of those two genes.
Viability and cell cycle evaluation confirmed that DON publicity decreased the IPEC-J2 viability (P < 0.01), blocked the cell cycle within the G2/M part (P < 0.01), and elevated the speed of apoptosis (P < 0.05). Expression of the SLC4A11 and MFSD3 genes was considerably downregulated upon DON publicity (P < 0.01).
Overexpression of SLC4A11 and MFSD3 can improve the cell viability (P < 0.01). DNA methylation assays indicated that promoter methylation of SLC4A11 (mC-1 and mC-23) and MFSD3 (mC-1 and mC-12) had been considerably increased in contrast with these within the controls and correlated negatively with mRNA expression (P < 0.05).
StemTAG PCR Primer Set for Stem Cell Characterization |
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| CBA-303 | Cell Biolabs | 1 kit | EUR 470.4 |
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Description: StemTAG PCR Primer Set for Stem Cell Characterization includes 7 primer pairs: Oct-4, NANOG, AFP, Flk-1, and NCAM, plus GAPDH and beta-actin as controls. |
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Prostate stem cell antigen |
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| AP86675 | SAB | 1mg | EUR 2640 |
Prostate stem cell antigen |
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| AP87071 | SAB | 1mg | EUR 2640 |
Mouse Psca/ Prostate stem cell antigen ELISA Kit |
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| E1215Mo | Sunlong | 1 Kit | EUR 543 |
Mouse Prostate stem cell antigen, Psca ELISA KIT |
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| ELI-22375m | Lifescience Market | 96 Tests | EUR 1038 |
Mouse Prostate stem cell antigen (PSCA) ELISA Kit |
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| abx520719-96tests | Abbexa | 96 tests | EUR 886.8 |
Mouse Prostate stem cell antigen (PSCA) ELISA Kit |
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| RK13577 | Abclonal | 96T | EUR 280 |
Mouse Psca / Prostate stem cell antigen ELISA Kit |
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| E8693m | EIAab | 96T | EUR 776 |
Total Protein - Murine Embryonic Stem Cell Line D3 |
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| CBA-305 | Cell Biolabs | 500 ?g | EUR 414 |
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Description:
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CytoSelect Proliferating Cell Nuclear Antigen (PCNA) ELISA Kit |
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| CBA-254 | Cell Biolabs | 96 assays | EUR 672 |
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Description: Cell Biolabs? CytoSelect Proliferating Cell Nuclear Antigen (PCNA) ELISA Kit is an enzyme immunoassay developed for the detection and quantitation of PCNA from nuclear and whole cell extracts. The kit detects PCNA from mouse, rat and human, and has a detection sensitivity limit of 12.5 ng/mLPCNA. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples. |
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Prostate Stem Cell Antigen Antibody |
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| 33476 | SAB | 100ul | EUR 319 |
Prostate Stem Cell Antigen Antibody |
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| 33476-100ul | SAB | 100ul | EUR 302.4 |
Prostate Stem Cell Antigen Antibody |
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| 33476-50ul | SAB | 50ul | EUR 224.4 |
Prostate Stem Cell Antigen Antibody |
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| E033476 | EnoGene | 100μg/100μl | EUR 255 |
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Description: Available in various conjugation types. |
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Prostate Stem Cell Antigen Antibody |
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| E19-8709-1 | EnoGene | 50ug/50ul | EUR 145 |
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Description: Available in various conjugation types. |
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Prostate Stem Cell Antigen Antibody |
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| E19-8709-2 | EnoGene | 100ug/100ul | EUR 225 |
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Description: Available in various conjugation types. |
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Prostate Stem Cell Antigen Antibody |
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| E11-0310C | EnoGene | 100μg/100μl | EUR 225 |
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Description: Available in various conjugation types. |
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Prostate Stem Cell Antigen Antibody |
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| DF8709 | Affbiotech | 200ul | EUR 420 |
Prostate Stem Cell Antigen Antibody |
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| DF8709-100ul | Affinity Biosciences | 100ul | EUR 280 |
Prostate Stem Cell Antigen Antibody |
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| DF8709-200ul | Affinity Biosciences | 200ul | EUR 350 |
Prostate Stem Cell Antigen Antibody |
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| ABD8709 | Lifescience Market | 100 ug | EUR 525.6 |
Prostate Stem Cell Antigen Antibody |
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| 8C0310 | AAT Bioquest | 50ug | EUR 368 |
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Description: Prostate Stem Cell Antigen Antibody |
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OPCA01291-20UG - Recombinant Mouse Prostate stem cell antigen |
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| OPCA01291-20UG | Aviva Systems Biology | 20ug | EUR 315 |
Psca (untagged) - Mouse prostate stem cell antigen (Psca), (10ug) |
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| MC211324 | Origene Technologies GmbH | 10 µg | Ask for price |
OPCA01291-100UG - Recombinant Mouse Prostate stem cell antigen |
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| OPCA01291-100UG | Aviva Systems Biology | 100ug | EUR 462 |
OPCA01291-500UG - Recombinant Mouse Prostate stem cell antigen |
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| OPCA01291-500UG | Aviva Systems Biology | 500ug | EUR 1276 |
Psca (GFP-tagged) - Mouse prostate stem cell antigen (Psca), (10ug) |
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| MG215317 | Origene Technologies GmbH | 10 µg | Ask for price |
Psca (Myc-DDK-tagged) - Mouse prostate stem cell antigen (Psca) |
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| MR215317 | Origene Technologies GmbH | 10 µg | Ask for price |
Prostate stem cell antigen (PSCA) Antibody |
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| 20-abx325516 | Abbexa |
|
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Prostate Stem Cell Antigen (PSCA) Antibody |
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| abx433189-200ul | Abbexa | 200 ul | EUR 343.2 |
Prostate Stem Cell Antigen (PSCA) Antibody |
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| 20-abx320478 | Abbexa |
|
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Prostate stem cell antigen (PSCA) Antibody |
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| abx331721-100ul | Abbexa | 100 ul | EUR 510 |
Prostate stem cell antigen (PSCA) Antibody |
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| 20-abx211961 | Abbexa |
|
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Prostate stem cell antigen (PSCA) Antibody |
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| 20-abx212351 | Abbexa |
|
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Prostate Stem Cell Antigen (PSCA) Antibody |
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| abx236848-100ug | Abbexa | 100 ug | EUR 661.2 |
Prostate Stem Cell Antigen (PSCA) Antibody |
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| 20-abx114737 | Abbexa |
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Prostate Stem Cell Antigen (PSCA) Antibody |
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| 20-abx102687 | Abbexa |
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Prostate Stem Cell Antigen (PSCA) Antibody |
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| 20-abx102688 | Abbexa |
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Prostate Stem Cell Antigen (PSCA) Antibody |
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| 20-abx132387 | Abbexa |
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Prostate Stem Cell Antigen (PSCA) Antibody |
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| 20-abx008545 | Abbexa |
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Prostate Stem Cell Antigen (PSCA) Antibody |
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| 20-abx013183 | Abbexa |
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Prostate Stem Cell Antigen (PSCA) Antibody |
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| abx027872-400ul | Abbexa | 400 ul | EUR 627.6 |
Prostate Stem Cell Antigen (PSCA) Antibody |
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| abx027872-80l | Abbexa | 80 µl | EUR 343.2 |
Prostate Stem Cell Antigen (PSCA), Antibody |
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| GWB-B3D35D | GenWay Biotech | 7 ml | Ask for price |
Prostate Stem Cell Antigen (PSCA), Antibody |
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| GWB-983A18 | GenWay Biotech | 1 ml | Ask for price |
Prostate Stem Cell Antigen (PSCA) Antibody |
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| abx004291-100l | Abbexa | 100 µl | EUR 400 |
Prostate Stem Cell Antigen (PSCA) Antibody |
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| abx004291-20l | Abbexa | 20 µl | EUR 175 |
Prostate Stem Cell Antigen (PSCA) Antibody |
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| abx004291-50l | Abbexa | 50 µl | EUR 275 |
Prostate Stem Cell Antigen (PSCA) Antibody |
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| abx013183-100g | Abbexa | 100 µg | EUR 237.5 |
Prostate Stem Cell Antigen (PSCA) Antibody |
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| abx013183-10g | Abbexa | 10 µg | EUR 43.75 |
Prostate Stem Cell Antigen (PSCA) Antibody |
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| abx013183-200g | Abbexa | 200 µg | EUR 312.5 |
Prostate Stem Cell Antigen (PSCA) Antibody |
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| abx236848-100g | Abbexa | 100 µg | EUR 350 |
Prostate Stem Cell Antigen (PSCA) Antibody |
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| abx114737-100l | Abbexa | 100 µl | EUR 612.5 |
Prostate Stem Cell Antigen (PSCA) Antibody |
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| abx132387-01mg | Abbexa | 0.1 mg | EUR 275 |
Prostate Stem Cell Antigen (PSCA) Antibody |
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| abx102687-100l | Abbexa | 100 µl | EUR 275 |
Prostate Stem Cell Antigen (PSCA) Antibody |
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| abx102687-1ml | Abbexa | 1 ml | EUR 750 |
Prostate Stem Cell Antigen (PSCA) Antibody |
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| abx102687-200l | Abbexa | 200 µl | EUR 337.5 |
Prostate Stem Cell Antigen (PSCA) Antibody |
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| abx102688-100l | Abbexa | 100 µl | EUR 275 |
Prostate Stem Cell Antigen (PSCA) Antibody |
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| abx102688-1ml | Abbexa | 1 ml | EUR 750 |
Prostate Stem Cell Antigen (PSCA) Antibody |
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| abx102688-200l | Abbexa | 200 µl | EUR 337.5 |
Prostate Stem Cell Antigen (PSCA) Antibody |
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| abx027872-400l | Abbexa | 400 µl | EUR 518.75 |
Prostate stem cell antigen (PSCA) Antibody |
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| abx211961-100l | Abbexa | 100 µl | EUR 350 |
Prostate stem cell antigen (PSCA) Antibody |
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| abx211961-50l | Abbexa | 50 µl | EUR 250 |
Prostate stem cell antigen (PSCA) Antibody |
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| abx212351-100l | Abbexa | 100 µl | EUR 350 |
Prostate stem cell antigen (PSCA) Antibody |
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| abx212351-50l | Abbexa | 50 µl | EUR 250 |
Prostate Stem Cell Antigen (PSCA) Antibody |
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| abx320478-100l | Abbexa | 100 µl | EUR 350 |
Prostate Stem Cell Antigen (PSCA) Antibody |
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| abx320478-50l | Abbexa | 50 µl | EUR 237.5 |
Prostate stem cell antigen (PSCA) Antibody |
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| abx325516-100g | Abbexa | 100 µg | EUR 250 |
Prostate stem cell antigen (PSCA) Antibody |
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| abx325516-50g | Abbexa | 50 µg | EUR 187.5 |
Prostate Stem Cell Antigen (PSCA) Antibody |
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| abx433189-100g | Abbexa | 100 µg | EUR 387.5 |
Human Prostate stem cell antigen (PSCA) |
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| 1-CSB-YP018840HU | Cusabio |
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|
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Description: Recombinant Human Prostate stem cell antigen(PSCA) expressed in Yeast |
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Human Prostate stem cell antigen (PSCA) |
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| 1-CSB-EP018840HU | Cusabio |
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|
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Description: Recombinant Human Prostate stem cell antigen(PSCA) expressed in E.coli |
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Human Prostate stem cell antigen (PSCA) |
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| 1-CSB-EP018840HUa0 | Cusabio |
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|
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Description: Recombinant Human Prostate stem cell antigen(PSCA) expressed in E.coli |
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Recombinant Mouse Prostate stem cell antigen Protein, His, Yeast-1mg |
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| QP9353-ye-1mg | EnQuireBio | 1mg | EUR 2763.6 |
Prostate Stem Cell Antigen Conjugated Antibody |
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| C33476 | SAB | 100ul | EUR 476.4 |
Recombinant Mouse Prostate stem cell antigen Protein, His, Yeast-100ug |
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| QP9353-ye-100ug | EnQuireBio | 100ug | EUR 685.2 |
Recombinant Mouse Prostate stem cell antigen Protein, His, Yeast-10ug |
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| QP9353-ye-10ug | EnQuireBio | 10ug | EUR 326.4 |
Recombinant Mouse Prostate stem cell antigen Protein, His, Yeast-200ug |
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| QP9353-ye-200ug | EnQuireBio | 200ug | EUR 1077.6 |
Recombinant Mouse Prostate stem cell antigen Protein, His, Yeast-500ug |
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| QP9353-ye-500ug | EnQuireBio | 500ug | EUR 1806 |
Recombinant Mouse Prostate stem cell antigen Protein, His, Yeast-50ug |
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| QP9353-ye-50ug | EnQuireBio | 50ug | EUR 424.8 |
Prostate Stem Cell Antigen Blocking Peptide |
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| DF8709-BP | Affbiotech | 1mg | EUR 234 |
Recombinant Prostate Stem Cell Antigen (PSCA) |
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| 4-RPC735Hu01 | Cloud-Clone |
|
|
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Description: Recombinant Human Prostate Stem Cell Antigen expressed in: E.coli |
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Recombinant Prostate Stem Cell Antigen (PSCA) |
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| RPC735Hu02 | Cloud-Clone | 10ug | EUR 168 |
Recombinant Prostate Stem Cell Antigen (PSCA) |
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| 4-RPC735Hu02 | Cloud-Clone |
|
|
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Description: Recombinant Human Prostate Stem Cell Antigen expressed in: E.coli |
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Recombinant Prostate Stem Cell Antigen (PSCA) |
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| RPU43449-100ug | Biomatik Corporation | 100ug | EUR 517 |
Recombinant Prostate Stem Cell Antigen (PSCA) |
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| RPU43449-1mg | Biomatik Corporation | 1mg | EUR 2293.2 |
Recombinant Prostate Stem Cell Antigen (PSCA) |
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| RPU43449-50ug | Biomatik Corporation | 50ug | EUR 415.8 |
Recombinant Prostate Stem Cell Antigen (PSCA) |
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| RPU43450-100ug | Biomatik Corporation | 100ug | EUR 517 |
Recombinant Prostate Stem Cell Antigen (PSCA) |
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| RPU43450-1mg | Biomatik Corporation | 1mg | EUR 2293.2 |
Recombinant Prostate Stem Cell Antigen (PSCA) |
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| RPU43450-50ug | Biomatik Corporation | 50ug | EUR 415.8 |
Prostate Stem Cell Antigen (PSCA) Antibody (FITC) |
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| 20-abx274396 | Abbexa |
|
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Prostate Stem Cell Antigen (PSCA) Antibody (FITC) |
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| 20-abx274397 | Abbexa |
|
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Prostate Stem Cell Antigen (PSCA) Antibody (FITC) |
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| abx274397-100tests | Abbexa | 100 tests | EUR 437.5 |
Human Prostate stem cell antigen ELISA Kit |
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| EK4789 | SAB | 96 tests | EUR 739 |
Human Prostate Stem Cell Antigen ELISA Kit |
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| IHUPSCAKT | Innovative research | each | EUR 733 |
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Description: Human Prostate Stem Cell Antigen ELISA Kit |
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Prostate Stem Cell Antigen (PSCA) Antibody (Biotin) |
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| 20-abx274360 | Abbexa |
|
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Prostate Stem Cell Antigen (PSCA) Antibody (Biotin) |
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| 20-abx272292 | Abbexa |
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Prostate Stem Cell Antigen (PSCA) Antibody (Biotin) |
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| abx272292-1ml | Abbexa | 1 ml | EUR 825 |
Prostate Stem Cell Antigen (PSCA) Antibody (Biotin) |
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| abx272292-200l | Abbexa | 200 µl | EUR 362.5 |
Human Prostate Stem Cell Antigen (PSCA) Protein |
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| 20-abx068705 | Abbexa |
|
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Human Prostate Stem Cell Antigen (PSCA) Protein |
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| 20-abx068706 | Abbexa |
|
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Human Prostate Stem Cell Antigen (PSCA) Protein |
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| abx068705-20g | Abbexa | 20 µg | EUR 387.5 |
Human Prostate Stem Cell Antigen (PSCA) Protein |
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| abx068705-5g | Abbexa | 5 µg | EUR 212.5 |
Human Prostate Stem Cell Antigen (PSCA) Protein |
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| abx068706-20g | Abbexa | 20 µg | EUR 387.5 |
Human Prostate Stem Cell Antigen (PSCA) Protein |
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| abx068706-5g | Abbexa | 5 µg | EUR 212.5 |
Prostate Stem Cell Antigen (PSCA) Monoclonal Antibody |
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| CAU29869-100ul | Biomatik Corporation | 100ul | EUR 248.6 |
Prostate Stem Cell Antigen (PSCA) Monoclonal Antibody |
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| CAU29869-200ul | Biomatik Corporation | 200ul | EUR 310.8 |
Prostate Stem Cell Antigen (PSCA) Polyclonal Antibody |
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| CAU23920-100ul | Biomatik Corporation | 100ul | EUR 235.2 |
Prostate Stem Cell Antigen (PSCA) Polyclonal Antibody |
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| CAU23920-200ul | Biomatik Corporation | 200ul | EUR 294 |
Prostate Stem Cell Antigen (PSCA) Polyclonal Antibody |
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| CAU23921-100ul | Biomatik Corporation | 100ul | EUR 235.2 |
Prostate Stem Cell Antigen (PSCA) Polyclonal Antibody |
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| CAU23921-200ul | Biomatik Corporation | 200ul | EUR 294 |
Recombinant Human Prostate stem cell antigen (PSCA) |
|||
| CSB-YP018840HU | Cusabio | 5938 mg | Ask for price |
Recombinant Human Prostate stem cell antigen (PSCA) |
|||
| CSB-EP018840HU | Cusabio | 1986 mg | Ask for price |
Recombinant Human Prostate stem cell antigen (PSCA) |
|||
| CSB-EP018840HUa0 | Cusabio | 9210 mg | Ask for price |
Recombinant Human Prostate stem cell antigen(PSCA) |
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| AP72177 | SAB | 1mg | EUR 1978 |
Recombinant Human Prostate stem cell antigen(PSCA) |
|||
| AP76667 | SAB | each | Ask for price |
Recombinant Human Prostate stem cell antigen(PSCA) |
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| AP70672 | SAB | 1mg | EUR 1978 |
Recombinant Human Prostate stem cell antigen (PSCA) |
|||
| RPC24902-100ug | Biomatik Corporation | 100ug | EUR 573.1 |
Recombinant Human Prostate stem cell antigen (PSCA) |
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| RPC24902-20ug | Biomatik Corporation | 20ug | EUR 288.8 |
Recombinant Human Prostate stem cell antigen (PSCA) |
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| RPC21869-100ug | Biomatik Corporation | 100ug | EUR 460.9 |
Recombinant Human Prostate stem cell antigen (PSCA) |
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| RPC21869-1mg | Biomatik Corporation | 1mg | EUR 2237.8 |
Additional evaluation confirmed that mC-1 of SLC4A11 and MFSD3 was situated in transcription issue binding websites for NF-1 and Sp1. Our findings revealed the novel organic capabilities of porcine SLC4A11 and MFSD3 genes in regulating the cytotoxic results induced by DON, and will contribute to the detection of biomarkers and drug targets for predicting and eliminating the potential toxicity of DON.