Exploiting Single-Cell Tools in Gene and Cell Therapy
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Exploiting Single-Cell Tools in Gene and Cell Therapy

- September 6, 2021 | October 26, 2023 - Ajan
Single-cell molecular instruments have been developed at an unimaginable tempo over the past 5 years as sequencing prices proceed to drop and quite a few molecular assays have been coupled to sequencing readouts. This fast interval of technological growth has facilitated the delineation of particular person molecular traits together with the genome, transcriptome, epigenome, and proteome of particular person cells, resulting in an unprecedented decision of the molecular networks governing complicated organic techniques.
The immense energy of single-cell molecular screens has been significantly highlighted by work in techniques the place mobile heterogeneity is a key characteristic, similar to stem cell biology, immunology, and tumor cell biology. Single-cell-omics applied sciences have already contributed to the identification of novel illness biomarkers, mobile subsets, therapeutic targets and diagnostics, a lot of which might have been undetectable by bulk sequencing approaches.
Extra not too long ago, efforts to combine single-cell multi-omics with single cell purposeful output and/or bodily location have been difficult however have led to substantial advances. Maybe most excitingly, there are rising alternatives to succeed in past the outline of static mobile states with current advances in modulation of cells by CRISPR expertise, particularly with the event of base editors which tremendously raises the prospect of cell and gene therapies.
On this overview, we offer a short overview of rising single-cell applied sciences and focus on present developments in integrating single-cell molecular screens and performing single-cell multi-omics for medical functions. We additionally focus on how single-cell molecular assays may be usefully mixed with purposeful information to unpick the mechanism of mobile decision-making. Lastly, we mirror upon the introduction of spatial transcriptomics and proteomics, its complementary function with single-cell RNA sequencing (scRNA-seq) and potential software in mobile and gene remedy.

Vector Methods to Actualize B Cell-Based mostly Gene Therapies

Latest developments in genome enhancing and supply techniques have opened new prospects for B cell gene remedy. CRISPR-Cas9 nucleases have been used to introduce transgenes into B cell genomes for subsequent secretion of exogenous therapeutic proteins from plasma cells and to program novel B cell Ag receptor specificities, permitting for the era of fascinating Ab responses that can’t usually be elicited in animal fashions.
Genome modification of B cells or their progenitor, hematopoietic stem cells, might probably substitute Ab or protein alternative therapies that require a number of injections over the long run. Thus far, B cell enhancing utilizing CRISPR-Cas9 has been solely employed in preclinical research, through which cells are edited ex vivo. On this overview, we focus on present B cell engineering efforts and techniques for the eventual protected and economical adoption of modified B cells into the clinic, together with in vivo viral supply of enhancing reagents to B cells.
Exploiting Single-Cell Tools in Gene and Cell Therapy

Analytics of host cell proteins (HCPs): classes from biopharmaceutical mAb evaluation for Gene remedy merchandise

Analytics for host cell protein (HCP) evaluation of therapeutic monoclonal antibody preparations have developed enormously. We think about how learnings from this could inform HCP evaluation of gene remedy viral vector merchandise. The appliance of mass spectrometry (MS) approaches for evaluation of HCPs in viral vector preparations is being established, though such data stays restricted and is but to be extensively utilized into course of or host cell line growth to cut back HCP quantities or danger.
As these MS approaches, and the information from them, are utilized and develop into accessible, the method understanding created will velocity course of growth exercise. We describe applied sciences which were, or may be, utilized to viral vector HCP evaluation to assist course of growth, scale back HCP quantities, determine crucial HCPs and thus inform danger evaluation and administration primarily based on a information of particular HCPs, in the end delivering protected and efficacious gene remedy merchandise to the clinic.

An optimum non-viral gene switch methodology for genetically modifying porcine bone marrow-derived endothelial progenitor cells for experimental therapeutics

No presently accessible therapy is ready to generate new contractile tissue or considerably enhance cardiac operate after myocardial infarction (MI), a number one reason behind morbidity and mortality worldwide. Though gene transfer-enhanced endothelial progenitor cells (GTE-EPCs) present effectiveness in MI therapy in small animal fashions, no medical trials utilizing GTE-EPCs have been documented. Earlier than the introduction of GTE-EPCs into human trials, gene-transfer-mediated augmentation of EPC operate in animal fashions that mirror the human MI state of affairs ought to be examined.
On this regard, a porcine mannequin is your best option since pigs have cardiac dimension, hemodynamics and coronary anatomy just like that of people. To look at GTE-EPC therapeutic efficacy in pig MI fashions, an environment friendly methodology for gene switch into pig EPCs is required, which nevertheless, has been poorly documented. Pig bone marrow mononuclear cells have been remoted and cultured in EGM-2 medium to acquire bone marrow-derived EPCs (BM-EPCs) that have been characterised by immunostaining and the tube formation assay.
Gene switch was optimized in 6-well plates utilizing a GFP and a VEGF plasmid, and scaled up in T75 flasks. Gene switch effectivity was decided by fluorescence microscopy and stream cytometry. VEGF ranges have been measured by ELISA. Cell proliferation was assayed by the CCK-Eight equipment. (1) BM-EPCs expressed VEGFR2 and eNOS however not CD45 protein, and shaped tube buildings on Matrigel; (2) a number of chemical compounds have been explored with the very best transfection effectivity of 41.4% ± 5.8% achieved utilizing Lipofectamine 3000; (3) the VEGF stage in tradition medium after VEGF transfection was 378 ± 48 ng/106 cells; and (4) BM-EPCs overexpressing VEGF had considerably enhanced proliferation than GFP-transfected EPCs.

Recombinant Mouse Betacellulin
cyt-131-5g 5µg
EUR 60

Recombinant Mouse Betacellulin
MBS144791-0005mg 0.005mg
EUR 240

Recombinant Mouse Betacellulin
MBS144791-002mg 0.02mg
EUR 310

Recombinant Mouse Betacellulin
MBS144791-1mg 1mg
EUR 2880

Recombinant Mouse Betacellulin
MBS144791-5x1mg 5x1mg
EUR 12630

Mouse Betacellulin ELISA Kit
IMSBTCKT each
EUR 702
Description: Mouse Betacellulin ELISA Kit

Mouse Betacellulin ELISA KIT
E42EM-172 96T/48T
EUR 795

Mouse Betacellulin ELISA Kit
MBS031949-10x96StripWells 10x96-Strip-Wells
EUR 6725

Mouse Betacellulin ELISA Kit
MBS031949-48StripWells 48-Strip-Wells
EUR 550

Mouse Betacellulin ELISA Kit
MBS031949-5x96StripWells 5x96-Strip-Wells
EUR 3420

Mouse Betacellulin ELISA Kit
MBS031949-96StripWells 96-Strip-Wells
EUR 765

Mouse betacellulin ELISA Kit
MBS3806344-10x96StripWells 10x96-Strip-Wells
EUR 6725

Mouse betacellulin ELISA Kit
MBS3806344-48StripWells 48-Strip-Wells
EUR 550

Mouse betacellulin ELISA Kit
MBS3806344-5x96StripWells 5x96-Strip-Wells
EUR 3420

Mouse betacellulin ELISA Kit
MBS3806344-96StripWells 96-Strip-Wells
EUR 765

Mouse Betacellulin ELISA Kit
MBS728526-10x96StripWells 10x96-Strip-Wells
EUR 5685

Mouse Betacellulin ELISA Kit
MBS728526-48StripWells 48-Strip-Wells
EUR 485

Mouse Betacellulin ELISA Kit
MBS728526-5x96StripWells 5x96-Strip-Wells
EUR 3020

Mouse Betacellulin ELISA Kit
MBS728526-96StripWells 96-Strip-Wells
EUR 690

Mouse Betacellulin ELISA Kit
MBS825066-5x96Tests 5x96Tests
EUR 2880

Mouse Betacellulin ELISA Kit
MBS825066-96Tests 96Tests
EUR 645

Mouse Betacellulin ELISA Kit
MBS8420397-1Kit 1Kit
EUR 755

Mouse Betacellulin ELISA Kit
MBS8420397-5x1Kit 5x1Kit
EUR 3455

Anti-Mouse Betacellulin Antibody
103-M308 100 µg
EUR 399
Description: Betacellulin (BTC) is a new member of the EGF family of cytokines that is comprised of at least ten proteins including EGF, TGFα, amphiregulin, HB-EGF, and the various heregulins. All of these cytokines are synthesized as transmembrane precursors and are characterized by the presence of one or more EGF structural units in their extracellular domain. The soluble forms of these cytokines are released by proteolytic cleavage. BTC, a heparin binding protein, was originally isolated from the conditioned media of mouse pancreatic beta tumor cells as a 32 kDa glycoprotein composed of 80 amino acid residues. The cDNA encoding human BTC was cloned from a human breast adenocarcinoma cell line (MCF7) cDNA library. Human and mouse cDNAs encode BTC precursor proteins of 178 and 177 amino acid residues, respectively. At the amino acid sequence level, human BTC precursor protein exhibits 79% identity with that of the mouse BTC precursor. In a mouse cell line transfected with human BTC cDNA, three forms of soluble human BTC have been detected: the glycosylated, intact BTC composed of 80 amino acid residues, a truncated molecule lacking 12 amino acid residues from the amino terminus, and a second truncated molecule lacking 30 amino acid residues from the amino-terminus. The biological activities of these BTC forms were shown to be identical. BTC can bind to the EGF receptor and is a potent mitogen for Balb/c 3T3 fibroblasts, retinal pigment epithelial cells and vascular smooth muscle cells.

Betacellulin Mouse Recombinant
rAP-0362 Inquiry Ask for price

Mouse Betacellulin (bTC) Protein
abx065525-100g 100 µg
EUR 612.5

Mouse Betacellulin (bTC) Protein
20-abx065525
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  • 10 ug
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  • 1 mg

Mouse Betacellulin (bTC) Protein
abx065525-10g 10 µg
EUR 237.5

Mouse Betacellulin (bTC) Protein
abx065525-50g 50 µg
EUR 425

Mouse Betacellulin (BTC) CLIA Kit
abx196745-100l 100 µl Ask for price

Mouse Betacellulin (BTC) CLIA Kit
abx196745-50l 50 µl
EUR 618.75

Mouse Betacellulin (BTC) CLIA Kit
20-abx491044
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  • 96 tests
  • 5 × 96 tests
  • 10 × 96 tests

anti- Betacellulin antibody
FNab00883 100µg
EUR 606.3
Description: Antibody raised against Betacellulin

Mouse Betacellulin (BTC) ELISA Kit
20-abx153708
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  • 96 tests
  • 5 × 96 tests
  • 10 × 96 tests

Mouse Betacellulin (BTC) ELISA Kit
abx574631-96tests 96 tests
EUR 801.6

Mouse Betacellulin (bTC) ELISA Kit
DLR-bTC-Mu 96T
EUR 370
Description: serum, plasma, tissue homogenates, cell lysates or other biological fluids.

Mouse Betacellulin (bTC) ELISA Kit
DLR-bTC-Mu-48T 48T
EUR 519.6
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Betacellulin (bTC) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids.

Mouse Betacellulin (bTC) ELISA Kit
DLR-bTC-Mu-96T 96T
EUR 669.6
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Betacellulin (bTC) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids.

Mouse Betacellulin (BTC) ELISA Kit
abx253974-96tests 96 tests
EUR 525

Mouse Betacellulin (bTC) ELISA Kit
DL-bTC-Mu 96T
EUR 353
Description: serum, plasma, tissue homogenates, cell lysates or other biological fluids.

Mouse Betacellulin (bTC) ELISA Kit
EKU02698-48T 48T
EUR 431.69

Mouse Betacellulin (bTC) ELISA Kit
EKU02698-5x96T 5x96T
EUR 2929.33

Mouse Betacellulin (bTC) ELISA Kit
EKU02698-96T 96T
EUR 616.7

Mouse Betacellulin/BTC ELISA Kit
EK5523 96 tests
EUR 599

Mouse Betacellulin (bTC) ELISA Kit
EKN43809-48T 48T
EUR 294

Mouse Betacellulin (bTC) ELISA Kit
EKN43809-5x96T 5x96T
EUR 1995

Mouse Betacellulin (bTC) ELISA Kit
EKN43809-96T 96T
EUR 420

Mouse Betacellulin (bTC) ELISA Kit
EK12924 96Т
EUR 768

Mouse Betacellulin (bTC) ELISA Kit
MBS452107-10x96StripWells 10x96-Strip-Wells
EUR 4225

Mouse Betacellulin (bTC) ELISA Kit
MBS452107-48StripWells 48-Strip-Wells
EUR 375

Mouse Betacellulin (bTC) ELISA Kit
MBS452107-5x96StripWells 5x96-Strip-Wells
EUR 2175

Mouse Betacellulin (bTC) ELISA Kit
MBS452107-96StripWells 96-Strip-Wells
EUR 490

Mouse Betacellulin (bTC) ELISA Kit
MBS457992-10x96Tests 10x96Tests
EUR 4405

Mouse Betacellulin (bTC) ELISA Kit
MBS457992-48Tests 48Tests
EUR 385

Mouse Betacellulin (bTC) ELISA Kit
MBS457992-5x96Tests 5x96Tests
EUR 2260

Mouse Betacellulin (bTC) ELISA Kit
MBS457992-96Tests 96Tests
EUR 510

Mouse Betacellulin (bTC) ELISA Kit
MBS2021662-10x96StripWells 10x96-Strip-Wells
EUR 4370

Mouse Betacellulin (bTC) ELISA Kit
MBS2021662-24StripWells 24-Strip-Wells
EUR 315

Mouse Betacellulin (bTC) ELISA Kit
MBS2021662-48StripWells 48-Strip-Wells
EUR 445

Mouse Betacellulin (bTC) ELISA Kit
MBS2021662-5x96StripWells 5x96-Strip-Wells
EUR 2390

Mouse Betacellulin (bTC) ELISA Kit
MBS2021662-96StripWells 96-Strip-Wells
EUR 590

Mouse Betacellulin (BTC) ELISA Kit
MBS2610691-10x96StripWells 10x96-Strip-Wells
EUR 3290

Mouse Betacellulin (BTC) ELISA Kit
MBS2610691-48StripWells 48-Strip-Wells
EUR 295

Mouse Betacellulin (BTC) ELISA Kit
MBS2610691-5x96StripWells 5x96-Strip-Wells
EUR 1800

Mouse Betacellulin (BTC) ELISA Kit
MBS2610691-96StripWells 96-Strip-Wells
EUR 450

Mouse Betacellulin (bTC) ELISA Kit
MBS2701249-10x96StripWells 10x96-Strip-Wells
EUR 2475

Mouse Betacellulin (bTC) ELISA Kit
MBS2701249-24StripWells 24-Strip-Wells
EUR 230

Mouse Betacellulin (bTC) ELISA Kit
MBS2701249-48StripWells 48-Strip-Wells
EUR 290

Mouse Betacellulin (bTC) ELISA Kit
MBS2701249-5x96StripWells 5x96-Strip-Wells
EUR 1290

Mouse Betacellulin (bTC) ELISA Kit
MBS2701249-96StripWells 96-Strip-Wells
EUR 355

Mouse Betacellulin (bTC) ELISA Kit
SEA112Mu-10x96wellstestplate 10x96-wells test plate
EUR 4452.58
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Mouse Betacellulin (bTC) ELISA Kit
SEA112Mu-1x48wellstestplate 1x48-wells test plate
EUR 471.86
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Mouse Betacellulin (bTC) ELISA Kit
SEA112Mu-1x96wellstestplate 1x96-wells test plate
EUR 622.66
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Mouse Betacellulin (bTC) ELISA Kit
SEA112Mu-5x96wellstestplate 5x96-wells test plate
EUR 2441.95
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Mouse Betacellulin (bTC) ELISA Kit
4-SEA112Mu
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  • 1 plate of 96 wells
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Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Betacellulin (bTC) in samples from Serum, plasma, tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse Betacellulin (bTC) ELISA Kit
RD-bTC-Mu-48T 48T
EUR 379
Description: sandwich

Mouse Betacellulin (bTC) ELISA Kit
RD-bTC-Mu-48Tests 48 Tests
EUR 511.2

Mouse Betacellulin (bTC) ELISA Kit
RD-bTC-Mu-96T 96T
EUR 542
Description: sandwich

Mouse Betacellulin (bTC) ELISA Kit
RD-bTC-Mu-96Tests 96 Tests
EUR 704.4

Mouse Betacellulin (bTC) ELISA Kit
RDR-bTC-Mu-48T 48T
EUR 398
Description: sandwich

Mouse Betacellulin (bTC) ELISA Kit
RDR-bTC-Mu-48Tests 48 Tests
EUR 534

Mouse Betacellulin (bTC) ELISA Kit
RDR-bTC-Mu-96T 96T
EUR 569
Description: sandwich

Mouse Betacellulin (bTC) ELISA Kit
RDR-bTC-Mu-96Tests 96 Tests
EUR 736.8

Mouse BTC -Betacellulin- CLIA Kit
E-CL-M0133-24Tests 24 Tests
EUR 180
Description: Sandwich

Mouse BTC -Betacellulin- CLIA Kit
E-CL-M0133-48Tests 48 Tests
EUR 546
Description: Sandwich

Mouse BTC -Betacellulin- CLIA Kit
E-CL-M0133-96Tests 96 Tests
EUR 682
Description: Sandwich

Mouse BTC -Betacellulin- CLIA Kit
E-CL-M0133-96Tests10 96 Tests *10
EUR 6820
Description: Sandwich

Mouse BTC -Betacellulin- CLIA Kit
E-CL-M0133-96Tests5 96 Tests *5
EUR 3410
Description: Sandwich

Mouse BTC (Betacellulin) ELISA Kit
MBS2511826-10x96Tests 10x96Tests
EUR 4060

Mouse BTC (Betacellulin) ELISA Kit
MBS2511826-24Tests 24Tests
EUR 265

Mouse BTC (Betacellulin) ELISA Kit
MBS2511826-48Tests 48Tests
EUR 450

Mouse BTC (Betacellulin) ELISA Kit
MBS2511826-5x96Tests 5x96Tests
EUR 2265

Mouse BTC (Betacellulin) ELISA Kit
MBS2511826-96Tests 96Tests
EUR 535

Mouse bTC (Betacellulin) ELISA Kit
MBS8800238-10x96StripWells 10x96-Strip-Wells
EUR 3130

Mouse bTC (Betacellulin) ELISA Kit
MBS8800238-48StripWells 48-Strip-Wells
EUR 315

Mouse bTC (Betacellulin) ELISA Kit
MBS8800238-5x96StripWells 5x96-Strip-Wells
EUR 1710

Mouse bTC (Betacellulin) ELISA Kit
MBS8800238-96StripWells 96-Strip-Wells
EUR 385

Mouse BTC(Betacellulin) ELISA Kit
RE2928M-48wellsplate 48-wells plate
EUR 126

Mouse BTC(Betacellulin) ELISA Kit
RE2928M-96wellsplate 96-wells plate
EUR 180

Recombinant Mouse Betacellulin protein
CD00612-5ug 5ug
EUR 176
Description: Betacellulin (BTC) encoded by the BTC gene located on the chromosome 4, is a member of the EGF family that also includes EGF, TGF-alpha, Amphiregulin, HB-EGF, Epiregulin, Tomoregulin and the Neuregulins. BTC is expressed in most tissues including kidney, uterus, liver and pancreas. It is also present in body fluids, including serum, milk, and colostrum. Mouse BTC is expressed as a 178-amino acid precursor and the amino acid sequence of the mature form is 80 % identical with human BTC. Both human and mouse BTC exhibit significant overall similarity with other members of the EGF family. Recombinant Mouse Betacellulin is a heparin binding protein containing 80 amino acids residues.

Mouse Betacellulin Recombinant Protein
M10-130 20 µg
EUR 196.35
Description: Betacellulin (BTC or beta-TC) is a member of the EGF family of cytokines which includes EGF, TGFα, amphiregulin, HB-EGF, epiregulin, tomoregulin and the neuregulins. All EGF famiy members are synthesized as type I transmembrane precursor proteins containing one or more EGF-like domains in their extracellular region. BTC, a heparin-binding protein, was originally isolated from the conditioned media of mouse pancreatic beta tumor cells as a 32 kDa glycoprotien. The mouse BTC cDNA encodes a 177 amino acid (aa) residue precursor with a 31 aa signal peptide, an 87 aa residue extracellular region containing one EGF-like domain, a 21 aa transmembrane domain and a 38 aa cytoplasmic domain. Soluble BTC is released from the transmembrane precursor by proteolytic processing. Mouse BTC shares 93% and 79% aa sequence identity with rat and human BTC, respectively. The mouse BTC gene is tightly linked to that of amphiregulin on mouse chromosome 5. BTC is expressed in most tissues including kidney, uterus, liver and pancreas. It is also present in body fluids including serum, milk and colostrum. BTC promotes pancreatic betacell growth and differentiation and is a potent mitogen for retinal pigment epithelial cells, vascular smooth muscle cells and fibroblasts. The effects of BTC is mediated by binding to ErbB1 and ErbB4 homodimers as well as ErbB heterodimers. Recombinant murine Betacellulin is a 9.0 kDa monomeric protein, containing 80 amino residues, which comprises the mature EGF homologous portion of the Betacellulin protein.

Mouse Betacellulin Recombinant Protein
M10-130S 5 µg
EUR 92.4
Description: Betacellulin (BTC or beta-TC) is a member of the EGF family of cytokines which includes EGF, TGFα, amphiregulin, HB-EGF, epiregulin, tomoregulin and the neuregulins. All EGF famiy members are synthesized as type I transmembrane precursor proteins containing one or more EGF-like domains in their extracellular region. BTC, a heparin-binding protein, was originally isolated from the conditioned media of mouse pancreatic beta tumor cells as a 32 kDa glycoprotien. The mouse BTC cDNA encodes a 177 amino acid (aa) residue precursor with a 31 aa signal peptide, an 87 aa residue extracellular region containing one EGF-like domain, a 21 aa transmembrane domain and a 38 aa cytoplasmic domain. Soluble BTC is released from the transmembrane precursor by proteolytic processing. Mouse BTC shares 93% and 79% aa sequence identity with rat and human BTC, respectively. The mouse BTC gene is tightly linked to that of amphiregulin on mouse chromosome 5. BTC is expressed in most tissues including kidney, uterus, liver and pancreas. It is also present in body fluids including serum, milk and colostrum. BTC promotes pancreatic betacell growth and differentiation and is a potent mitogen for retinal pigment epithelial cells, vascular smooth muscle cells and fibroblasts. The effects of BTC is mediated by binding to ErbB1 and ErbB4 homodimers as well as ErbB heterodimers. Recombinant murine Betacellulin is a 9.0 kDa monomeric protein, containing 80 amino residues, which comprises the mature EGF homologous portion of the Betacellulin protein.

Rat Monoclonal anti-mouse Betacellulin
mAP-0154 100ug
EUR 250

Betacellulin (HEK293-expressed), Mouse
HY-P7142 50ug
EUR 639.6

Betacellulin (bTC) Polyclonal Antibody (Mouse)
4-PAA112Mu01
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Description: A Rabbit polyclonal antibody against Mouse Betacellulin (bTC)

CLIA kit for Mouse BTC (Betacellulin)
E-CL-M0133 1 plate of 96 wells
EUR 700.8
Description: A sandwich CLIA kit for quantitative measurement of Mouse BTC (Betacellulin) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Mouse BTC (Betacellulin)
E-EL-M0181 1 plate of 96 wells
EUR 640.8
Description: A sandwich ELISA kit for quantitative measurement of Mouse BTC (Betacellulin) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Mouse bTC (Betacellulin)
ELK1236 1 plate of 96 wells
EUR 446.4
Description: A sandwich ELISA kit for detection of Betacellulin from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Anti-BTC/Betacellulin Antibody
MBS2400715-005mg 0.05mg
EUR 595

Anti-BTC/Betacellulin Antibody
MBS2400715-5x005mg 5x0.05mg
EUR 2500

EasyPro Mouse Betacellulin(BTC)ELISA Kit
FY-EM2928S each
EUR 520
Description: 4.69 pg/mL

Betacellulin (bTC) Polyclonal Antibody (Mouse), PE
4-PAA112Mu01-PE
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Description: A Rabbit polyclonal antibody against Mouse Betacellulin (bTC). This antibody is labeled with PE.

Mouse Betacellulin/BTC PicoKine ELISA Kit
MBS177297-5x96StripWells 5x96-Strip-Wells
EUR 2755

Mouse Betacellulin/BTC PicoKine ELISA Kit
MBS177297-96StripWells 96-Strip-Wells
EUR 600

Anti-Human Betacellulin Antibody
102-P222 100 µg
EUR 245.7
Description: Betacellulin is an EGF-related polypeptide growth factor that signals through the EGF receptor. It is produced in several tissues, including the pancreas, small intestine, and in certain tumor cells. Betacellulin is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. Human Betacellulin is initially synthesized as a glycosylated 32.0 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce the mature sequence. Recombinant human Betacellulin is a 9.0 kDa monomeric protein, containing 80 amino residues, which comprises the mature EGF homologous portion of the Betacellulin protein.

Anti-Human Betacellulin Antibody
101-M143 100 µg
EUR 399
Description: Betacellulin (BTC) is a new member of the EGF family of cytokines that is comprised of at least ten proteins including EGF, TGF αlpha, amphiregulin, HBEGF, and the various heregulins. All of these cytokines are synthesized as transmembrane precursors and are characterized by the presence of one or more EGF structural units in their extracellular domain. The soluble forms of these cytokines are released by proteolytic cleavage. BTC, a heparin binding protein, was originally isolated from the conditioned media of mouse pancreatic beta tumor cells as a 32 kDa glycoprotein composed of 80 amino acid residues. The cDNA encoding human BTC was cloned from a human breast adenocarcinoma cell line (MCF7) cDNA library. Human and mouse cDNAs encode BTC precursor proteins of 178 and 177 amino acid residues, respectively. At the amino acid sequence level, human BTC precursor protein exhibits 79% identity with that of the mouse BTC precursor. In a mouse cell line transfected with human BTC cDNA, three forms of soluble human BTC have been detected: the glycosylated, intact BTC composed of 80 amino acid residues, a truncated molecule lacking 12 amino acid residues from the amino terminus, and a second truncated molecule lacking 30 amino acid residues from the amino terminus. The biological activities of these BTC forms were shown to be identical. BTC can bind to the EGF receptor and is a potent mitogen for Balb/c 3T3 fibroblasts, retinal pigment epithelial cells and vascular smooth muscle cells.

Anti-Human Betacellulin Antibody
101-M230 100 µg
EUR 399
Description: Betacellulin (BTC) is a new member of the EGF family of cytokines that is comprised of at least ten proteins including EGF, TGF αlpha, amphiregulin, HBEGF, and the various heregulins. All of these cytokines are synthesized as transmembrane precursors and are characterized by the presence of one or more EGF structural units in their extracellular domain. The soluble forms of these cytokines are released by proteolytic cleavage. BTC, a heparin binding protein, was originally isolated from the conditioned media of mouse pancreatic beta tumor cells as a 32 kDa glycoprotein composed of 80 amino acid residues. The cDNA encoding human BTC was cloned from a human breast adenocarcinoma cell line (MCF7) cDNA library. Human and mouse cDNAs encode BTC precursor proteins of 178 and 177 amino acid residues, respectively. At the amino acid sequence level, human BTC precursor protein exhibits 79% identity with that of the mouse BTC precursor. In a mouse cell line transfected with human BTC cDNA, three forms of soluble human BTC have been detected: the glycosylated, intact BTC composed of 80 amino acid residues, a truncated molecule lacking 12 amino acid residues from the amino terminus, and a second truncated molecule lacking 30 amino acid residues from the amino terminus. The biological activities of these BTC forms were shown to be identical. BTC can bind to the EGF receptor and is a potent mitogen for Balb/c 3T3 fibroblasts, retinal pigment epithelial cells and vascular smooth muscle cells.

Betacellulin (bTC) Polyclonal Antibody (Mouse), APC
4-PAA112Mu01-APC
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Description: A Rabbit polyclonal antibody against Mouse Betacellulin (bTC). This antibody is labeled with APC.

Betacellulin (bTC) Polyclonal Antibody (Mouse), Cy3
4-PAA112Mu01-Cy3
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  • 200ul
  • 1ml
  • 10ml
Description: A Rabbit polyclonal antibody against Mouse Betacellulin (bTC). This antibody is labeled with Cy3.

Betacellulin (bTC) Polyclonal Antibody (Mouse), HRP
4-PAA112Mu01-HRP
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  • 20ul
  • 100ul
  • 200ul
  • 1ml
  • 10ml
Description: A Rabbit polyclonal antibody against Mouse Betacellulin (bTC). This antibody is labeled with HRP.

Betacellulin (bTC) Polyclonal Antibody (Mouse), FITC
4-PAA112Mu01-FITC
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  • 20ul
  • 100ul
  • 200ul
  • 1ml
  • 10ml
Description: A Rabbit polyclonal antibody against Mouse Betacellulin (bTC). This antibody is labeled with FITC.

MousebTC(Betacellulin)ELISAKit
ELK1236-48T 48T
EUR 160
Description: Sandwich

MousebTC(Betacellulin)ELISAKit
ELK1236-96T 96T
EUR 229
Description: Sandwich

BTC Betacellulin Mouse Recombinant Protein
PROTQ05928 Regular: 20ug
EUR 250
Description: BTC Mouse Recombinant produced in E. coli is a single, non-glycosylated polypeptide chain containing 80 amino acids and having a molecular mass of 9.0kDa. The BTC is purified by proprietary chromatographic techniques.

Recombinant Mouse Betacellulin/BTC (N-6His)
C747-10ug 10ug
EUR 187.2
Description: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.

Recombinant Mouse Betacellulin/BTC (N-6His)
C747-1mg 1mg
EUR 2739.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.

Recombinant Mouse Betacellulin/BTC (N-6His)
C747-500ug 500ug
EUR 1935.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.

Recombinant Mouse Betacellulin/BTC (N-6His)
C747-50ug 50ug
EUR 442.8
Description: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.

Recombinant Mouse Betacellulin/BTC (N-6His)
AP75333 1mg
EUR 3209

Mouse Monoclonal ant-Human Betacellulin (BTC)
hAP-0110 100ug
EUR 250

Mouse Monoclonal ant-Human Betacellulin (BTC)
hAP-0110A 100ug
EUR 250

Mouse Betacellulin/BTC(Beta Cellulin) ELISA Kit
EM0021 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 4.688pg/ml

Betacellulin/BTC ELISA Kit (Mouse) (OKBB00530)
OKBB00530 96 Wells
EUR 606
Description: Description of target: Betacellulin, also known as BTC is a protein that in humans is encoded by the BTC gene. This gene is mapped to 4q13.3. The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. It is the growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. This protein is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.;Species reactivity: Mouse;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: <= 10 pg/mL

Betacellulin antibody
70R-BR013 50 ug
EUR 312
Description: Affinity purified Rabbit polyclonal Betacellulin antibody

Betacellulin antibody
MBS535912-005mg 0.05mg
EUR 465

Betacellulin antibody
MBS535912-5x005mg 5x0.05mg
EUR 1945

Mouse Betacellulin/BTC ELISA Kit PicoKine®
EK1193 96 wells
EUR 499
Description: Mouse Betacellulin/BTC ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Btc in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Mouse Betacellulin HEK293 Overexpression Lysate
MBS8116554-03mg 0.3mg
EUR 280

Mouse Betacellulin HEK293 Overexpression Lysate
MBS8116554-5x03mg 5x0.3mg
EUR 1205

Betacellulin (BTC)
THP-0274 1 vial
EUR 3198.4
Description: Protein

Mouse Betacellulin Recombinant Protein Lyophilized
IMSBTCRLY5UG each
EUR 341
Description: Mouse Betacellulin Recombinant Protein Lyophilized

Mouse Betacellulin Recombinant Protein Lyophilized
MBS8433954-INQUIRE INQUIRE Ask for price

Betacellulin (bTC) Polyclonal Antibody (Mouse), APC-Cy7
4-PAA112Mu01-APC-Cy7
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  • 20ul
  • 100ul
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  • 1ml
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Description: A Rabbit polyclonal antibody against Mouse Betacellulin (bTC). This antibody is labeled with APC-Cy7.

Mouse Betacellulin Chemiluminescent Immunoassay Kit
IMSBTCKTC each
EUR 545
Description: Mouse Betacellulin Chemiluminescent Immunoassay Kit

Mouse Betacellulin Flow Cytometry Assay - Group 3
MX78121 96 Tests
EUR 270

Betacellulin, Human
HY-P7005 10ug
EUR 253.2

Human Betacellulin
MBS691789-0005mg 0.005mg
EUR 300

Human Betacellulin
MBS691789-002mg 0.02mg
EUR 450

Human Betacellulin
MBS691789-5x002mg 5x0.02mg
EUR 1725

Betacellulin, human
RC218-23 5ug
EUR 125.26

Betacellulin (bTC) Polyclonal Antibody (Mouse), Biotinylated
4-PAA112Mu01-Biotin
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  • 20ul
  • 100ul
  • 200ul
  • 1ml
  • 10ml
Description: A Rabbit polyclonal antibody against Mouse Betacellulin (bTC). This antibody is labeled with Biotin.

Bovine Betacellulin
ARU020 20 µg
EUR 303.6

Bovine Betacellulin
ARU100 100 µg
EUR 80

Recombinant Mouse Betacellulin/BTC Protein (His Tag)
PKSM040967-10ug 10ug
EUR 129
Description: Mouse

Recombinant Mouse Betacellulin/BTC Protein (His Tag)
PKSM040967-50ug 50ug
EUR 375
Description: Mouse

Betacellulin (BTC) Antibody
abx111218-100l 100 µl
EUR 612.5

Betacellulin (BTC) Antibody
20-abx111218
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  • 50 ul
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Betacellulin (bTC) Antibody
abx100739-100l 100 µl
EUR 237.5

Betacellulin (bTC) Antibody
20-abx100739
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  • 10 ug
  • 50 ug
  • 100 ug
  • 200 ug
  • 1 mg

Betacellulin (bTC) Antibody
abx100739-1ml 1 ml
EUR 575

Betacellulin (bTC) Antibody
abx100739-200l 200 µl
EUR 287.5

Betacellulin (bTC) Antibody
abx100740-100l 100 µl
EUR 250

Betacellulin (bTC) Antibody
20-abx100740
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  • 10 ug
  • 50 ug
  • 100 ug
  • 200 ug
  • 1 mg

Betacellulin (bTC) Antibody
abx100740-1ml 1 ml
EUR 675

Betacellulin (bTC) Antibody
abx100740-200l 200 µl
EUR 312.5

Betacellulin (bTC) Antibody
20-abx175568
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  • 200 ug
  • 1 mg

Betacellulin (bTC) Antibody
20-abx175569
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  • 200 ug
  • 1 mg

Betacellulin (bTC) Antibody
abx175570-1096tests 10 × 96 tests Ask for price

Betacellulin (bTC) Antibody
20-abx175570
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  • 200 ug
  • 1 mg

Betacellulin (bTC) Antibody
abx175570-596tests 5 × 96 tests Ask for price

Betacellulin (bTC) Antibody
abx175570-96tests 96 tests
EUR 725

Betacellulin (bTC) Antibody
20-abx175571
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  • 200 ug
  • 1 mg

Betacellulin (bTC) Antibody
abx171411-100l 100 µl
EUR 712.5

Betacellulin (bTC) Antibody
20-abx171411
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  • 200 ug
  • 1 mg

Betacellulin (bTC) Antibody
abx171411-1ml 1 ml Ask for price
A easy, straightforward and low cost methodology that may be utilized to supply a lot of genetically-modified BM-EPCs was established, which can facilitate the research of GTE-EPC therapeutic efficacy in pig MI mannequin.

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