Single-cell molecular instruments have been developed at an unimaginable tempo over the past 5 years as sequencing prices proceed to drop and quite a few molecular assays have been coupled to sequencing readouts. This fast interval of technological growth has facilitated the delineation of particular person molecular traits together with the genome, transcriptome, epigenome, and proteome of particular person cells, resulting in an unprecedented decision of the molecular networks governing complicated organic techniques.
The immense energy of single-cell molecular screens has been significantly highlighted by work in techniques the place mobile heterogeneity is a key characteristic, similar to stem cell biology, immunology, and tumor cell biology. Single-cell-omics applied sciences have already contributed to the identification of novel illness biomarkers, mobile subsets, therapeutic targets and diagnostics, a lot of which might have been undetectable by bulk sequencing approaches.
Extra not too long ago, efforts to combine single-cell multi-omics with single cell purposeful output and/or bodily location have been difficult however have led to substantial advances. Maybe most excitingly, there are rising alternatives to succeed in past the outline of static mobile states with current advances in modulation of cells by CRISPR expertise, particularly with the event of base editors which tremendously raises the prospect of cell and gene therapies.
On this overview, we offer a short overview of rising single-cell applied sciences and focus on present developments in integrating single-cell molecular screens and performing single-cell multi-omics for medical functions. We additionally focus on how single-cell molecular assays may be usefully mixed with purposeful information to unpick the mechanism of mobile decision-making. Lastly, we mirror upon the introduction of spatial transcriptomics and proteomics, its complementary function with single-cell RNA sequencing (scRNA-seq) and potential software in mobile and gene remedy.
Vector Methods to Actualize B Cell-Based mostly Gene Therapies
Latest developments in genome enhancing and supply techniques have opened new prospects for B cell gene remedy. CRISPR-Cas9 nucleases have been used to introduce transgenes into B cell genomes for subsequent secretion of exogenous therapeutic proteins from plasma cells and to program novel B cell Ag receptor specificities, permitting for the era of fascinating Ab responses that can’t usually be elicited in animal fashions.
Genome modification of B cells or their progenitor, hematopoietic stem cells, might probably substitute Ab or protein alternative therapies that require a number of injections over the long run. Thus far, B cell enhancing utilizing CRISPR-Cas9 has been solely employed in preclinical research, through which cells are edited ex vivo. On this overview, we focus on present B cell engineering efforts and techniques for the eventual protected and economical adoption of modified B cells into the clinic, together with in vivo viral supply of enhancing reagents to B cells.
Analytics of host cell proteins (HCPs): classes from biopharmaceutical mAb evaluation for Gene remedy merchandise
Analytics for host cell protein (HCP) evaluation of therapeutic monoclonal antibody preparations have developed enormously. We think about how learnings from this could inform HCP evaluation of gene remedy viral vector merchandise. The appliance of mass spectrometry (MS) approaches for evaluation of HCPs in viral vector preparations is being established, though such data stays restricted and is but to be extensively utilized into course of or host cell line growth to cut back HCP quantities or danger.
As these MS approaches, and the information from them, are utilized and develop into accessible, the method understanding created will velocity course of growth exercise. We describe applied sciences which were, or may be, utilized to viral vector HCP evaluation to assist course of growth, scale back HCP quantities, determine crucial HCPs and thus inform danger evaluation and administration primarily based on a information of particular HCPs, in the end delivering protected and efficacious gene remedy merchandise to the clinic.
An optimum non-viral gene switch methodology for genetically modifying porcine bone marrow-derived endothelial progenitor cells for experimental therapeutics
No presently accessible therapy is ready to generate new contractile tissue or considerably enhance cardiac operate after myocardial infarction (MI), a number one reason behind morbidity and mortality worldwide. Though gene transfer-enhanced endothelial progenitor cells (GTE-EPCs) present effectiveness in MI therapy in small animal fashions, no medical trials utilizing GTE-EPCs have been documented. Earlier than the introduction of GTE-EPCs into human trials, gene-transfer-mediated augmentation of EPC operate in animal fashions that mirror the human MI state of affairs ought to be examined.
On this regard, a porcine mannequin is your best option since pigs have cardiac dimension, hemodynamics and coronary anatomy just like that of people. To look at GTE-EPC therapeutic efficacy in pig MI fashions, an environment friendly methodology for gene switch into pig EPCs is required, which nevertheless, has been poorly documented. Pig bone marrow mononuclear cells have been remoted and cultured in EGM-2 medium to acquire bone marrow-derived EPCs (BM-EPCs) that have been characterised by immunostaining and the tube formation assay.
Gene switch was optimized in 6-well plates utilizing a GFP and a VEGF plasmid, and scaled up in T75 flasks. Gene switch effectivity was decided by fluorescence microscopy and stream cytometry. VEGF ranges have been measured by ELISA. Cell proliferation was assayed by the CCK-Eight equipment. (1) BM-EPCs expressed VEGFR2 and eNOS however not CD45 protein, and shaped tube buildings on Matrigel; (2) a number of chemical compounds have been explored with the very best transfection effectivity of 41.4% ± 5.8% achieved utilizing Lipofectamine 3000; (3) the VEGF stage in tradition medium after VEGF transfection was 378 ± 48 ng/106 cells; and (4) BM-EPCs overexpressing VEGF had considerably enhanced proliferation than GFP-transfected EPCs.
Recombinant Mouse Betacellulin |
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| cyt-131-5g | ProSpec Tany | 5µg | EUR 60 |
Recombinant Mouse Betacellulin |
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| MBS144791-0005mg | MyBiosource | 0.005mg | EUR 240 |
Recombinant Mouse Betacellulin |
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| MBS144791-002mg | MyBiosource | 0.02mg | EUR 310 |
Recombinant Mouse Betacellulin |
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| MBS144791-1mg | MyBiosource | 1mg | EUR 2880 |
Recombinant Mouse Betacellulin |
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| MBS144791-5x1mg | MyBiosource | 5x1mg | EUR 12630 |
Mouse Betacellulin ELISA KIT |
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| E42EM-172 | EnoGene | 96T/48T | Ask for price |
Mouse Betacellulin ELISA Kit |
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| MBS031949-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 6725 |
Mouse Betacellulin ELISA Kit |
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| MBS031949-48StripWells | MyBiosource | 48-Strip-Wells | EUR 550 |
Mouse Betacellulin ELISA Kit |
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| MBS031949-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3420 |
Mouse Betacellulin ELISA Kit |
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| MBS031949-96StripWells | MyBiosource | 96-Strip-Wells | EUR 765 |
Mouse Betacellulin ELISA Kit |
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| IMSBTCKT | Innovative research | each | EUR 702 |
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Description: Mouse Betacellulin ELISA Kit |
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Mouse Betacellulin ELISA Kit |
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| MBS728526-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 5685 |
Mouse Betacellulin ELISA Kit |
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| MBS728526-48StripWells | MyBiosource | 48-Strip-Wells | EUR 485 |
Mouse Betacellulin ELISA Kit |
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| MBS728526-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3020 |
Mouse Betacellulin ELISA Kit |
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| MBS728526-96StripWells | MyBiosource | 96-Strip-Wells | EUR 690 |
Mouse Betacellulin ELISA Kit |
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| MBS8420397-1Kit | MyBiosource | 1Kit | EUR 755 |
Mouse Betacellulin ELISA Kit |
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| MBS8420397-5x1Kit | MyBiosource | 5x1Kit | EUR 3455 |
Mouse Betacellulin ELISA Kit |
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| MBS825066-5x96Tests | MyBiosource | 5x96Tests | EUR 2880 |
Mouse Betacellulin ELISA Kit |
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| MBS825066-96Tests | MyBiosource | 96Tests | EUR 645 |
Mouse betacellulin ELISA Kit |
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| MBS3806344-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 6725 |
Mouse betacellulin ELISA Kit |
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| MBS3806344-48StripWells | MyBiosource | 48-Strip-Wells | EUR 550 |
Mouse betacellulin ELISA Kit |
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| MBS3806344-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3420 |
Mouse betacellulin ELISA Kit |
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| MBS3806344-96StripWells | MyBiosource | 96-Strip-Wells | EUR 765 |
Anti-Mouse Betacellulin Antibody |
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| 103-M308 | ReliaTech | 100 µg | EUR 399 |
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Description: Betacellulin (BTC) is a new member of the EGF family of cytokines that is comprised of at least ten proteins including EGF, TGFα, amphiregulin, HB-EGF, and the various heregulins. All of these cytokines are synthesized as transmembrane precursors and are characterized by the presence of one or more EGF structural units in their extracellular domain. The soluble forms of these cytokines are released by proteolytic cleavage. BTC, a heparin binding protein, was originally isolated from the conditioned media of mouse pancreatic beta tumor cells as a 32 kDa glycoprotein composed of 80 amino acid residues. The cDNA encoding human BTC was cloned from a human breast adenocarcinoma cell line (MCF7) cDNA library. Human and mouse cDNAs encode BTC precursor proteins of 178 and 177 amino acid residues, respectively. At the amino acid sequence level, human BTC precursor protein exhibits 79% identity with that of the mouse BTC precursor. In a mouse cell line transfected with human BTC cDNA, three forms of soluble human BTC have been detected: the glycosylated, intact BTC composed of 80 amino acid residues, a truncated molecule lacking 12 amino acid residues from the amino terminus, and a second truncated molecule lacking 30 amino acid residues from the amino-terminus. The biological activities of these BTC forms were shown to be identical. BTC can bind to the EGF receptor and is a potent mitogen for Balb/c 3T3 fibroblasts, retinal pigment epithelial cells and vascular smooth muscle cells. |
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Betacellulin Mouse Recombinant |
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| rAP-0362 | Angio Proteomie | Inquiry | Ask for price |
Mouse Betacellulin (bTC) Protein |
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| 20-abx065525 | Abbexa |
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Mouse Betacellulin (bTC) Protein |
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| abx065525-100g | Abbexa | 100 µg | EUR 612.5 |
Mouse Betacellulin (bTC) Protein |
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| abx065525-10g | Abbexa | 10 µg | EUR 237.5 |
Mouse Betacellulin (bTC) Protein |
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| abx065525-50g | Abbexa | 50 µg | EUR 425 |
Mouse Betacellulin (BTC) CLIA Kit |
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| 20-abx491044 | Abbexa |
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Mouse Betacellulin (BTC) CLIA Kit |
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| abx196745-100l | Abbexa | 100 µl | Ask for price |
Mouse Betacellulin (BTC) CLIA Kit |
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| abx196745-50l | Abbexa | 50 µl | EUR 618.75 |
anti- Betacellulin antibody |
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| FNab00883 | FN Test | 100µg | EUR 606.3 |
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Description: Antibody raised against Betacellulin |
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Mouse Betacellulin (BTC) ELISA Kit |
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| abx253974-96tests | Abbexa | 96 tests | EUR 801.6 |
Mouse Betacellulin (BTC) ELISA Kit |
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| abx574631-96tests | Abbexa | 96 tests | EUR 801.6 |
Mouse Betacellulin (BTC) ELISA Kit |
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| 20-abx153708 | Abbexa |
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Mouse Betacellulin (bTC) ELISA Kit |
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| DLR-bTC-Mu | DL Develop | 96T | EUR 370 |
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Description: serum, plasma, tissue homogenates, cell lysates or other biological fluids. |
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Mouse Betacellulin (bTC) ELISA Kit |
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| DLR-bTC-Mu-48T | DL Develop | 48T | EUR 519.6 |
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Description: A sandwich quantitative ELISA assay kit for detection of Mouse Betacellulin (bTC) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids. |
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Mouse Betacellulin (bTC) ELISA Kit |
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| DLR-bTC-Mu-96T | DL Develop | 96T | EUR 669.6 |
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Description: A sandwich quantitative ELISA assay kit for detection of Mouse Betacellulin (bTC) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids. |
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Mouse Betacellulin (bTC) ELISA Kit |
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| EKN43809-48T | Biomatik Corporation | 48T | EUR 294 |
Mouse Betacellulin (bTC) ELISA Kit |
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| EKN43809-5x96T | Biomatik Corporation | 5x96T | EUR 1995 |
Mouse Betacellulin (bTC) ELISA Kit |
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| EKN43809-96T | Biomatik Corporation | 96T | EUR 420 |
Mouse Betacellulin (bTC) ELISA Kit |
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| EKU02698-48T | Biomatik Corporation | 48T | EUR 431.69 |
Mouse Betacellulin (bTC) ELISA Kit |
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| EKU02698-5x96T | Biomatik Corporation | 5x96T | EUR 2929.33 |
Mouse Betacellulin (bTC) ELISA Kit |
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| EKU02698-96T | Biomatik Corporation | 96T | EUR 616.7 |
Mouse Betacellulin (bTC) ELISA Kit |
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| DL-bTC-Mu | DL Develop | 96T | EUR 353 |
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Description: serum, plasma, tissue homogenates, cell lysates or other biological fluids. |
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Mouse Betacellulin (bTC) ELISA Kit |
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| EK12924 | SAB | 96Т | EUR 768 |
Mouse Betacellulin/BTC ELISA Kit |
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| EK5523 | SAB | 96 tests | EUR 599 |
Mouse Betacellulin (bTC) ELISA Kit |
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| SEA112Mu-10x96wellstestplate | Cloud-Clone | 10x96-wells test plate | EUR 4452.58 |
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids. |
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Mouse Betacellulin (bTC) ELISA Kit |
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| SEA112Mu-1x48wellstestplate | Cloud-Clone | 1x48-wells test plate | EUR 471.86 |
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids. |
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Mouse Betacellulin (bTC) ELISA Kit |
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| SEA112Mu-1x96wellstestplate | Cloud-Clone | 1x96-wells test plate | EUR 622.66 |
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids. |
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Mouse Betacellulin (bTC) ELISA Kit |
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| SEA112Mu-5x96wellstestplate | Cloud-Clone | 5x96-wells test plate | EUR 2441.95 |
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids. |
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Mouse Betacellulin (bTC) ELISA Kit |
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| 4-SEA112Mu | Cloud-Clone |
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Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Betacellulin (bTC) in samples from Serum, plasma, tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. |
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Mouse Betacellulin/BTC ELISA Kit |
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| EF012837 | Nova Lifetech | 96tests | EUR 566 |
Mouse Betacellulin (bTC) ELISA Kit |
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| RD-bTC-Mu-48T | Reddot Biotech | 48T | EUR 361.7 |
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Description: serum, plasma, tissue homogenates, cell lysates and other biological fluids. |
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Mouse Betacellulin (bTC) ELISA Kit |
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| RD-bTC-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 511.2 |
Mouse Betacellulin (bTC) ELISA Kit |
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| RD-bTC-Mu-96T | Reddot Biotech | 96T | EUR 516.7 |
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Description: serum, plasma, tissue homogenates, cell lysates and other biological fluids. |
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Mouse Betacellulin (bTC) ELISA Kit |
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| RD-bTC-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 704.4 |
Mouse Betacellulin (bTC) ELISA Kit |
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| RDR-bTC-Mu-48T | Reddot Biotech | 48T | EUR 379.79 |
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Description: serum, plasma, tissue homogenates, cell lysates and other biological fluids. |
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Mouse Betacellulin (bTC) ELISA Kit |
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| RDR-bTC-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 534 |
Mouse Betacellulin (bTC) ELISA Kit |
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| RDR-bTC-Mu-96T | Reddot Biotech | 96T | EUR 542.54 |
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Description: serum, plasma, tissue homogenates, cell lysates and other biological fluids. |
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Mouse Betacellulin (bTC) ELISA Kit |
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| RDR-bTC-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 736.8 |
Mouse Betacellulin (bTC) ELISA Kit |
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| MBS2021662-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 4370 |
Mouse Betacellulin (bTC) ELISA Kit |
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| MBS2021662-24StripWells | MyBiosource | 24-Strip-Wells | EUR 315 |
Mouse Betacellulin (bTC) ELISA Kit |
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| MBS2021662-48StripWells | MyBiosource | 48-Strip-Wells | EUR 445 |
Mouse Betacellulin (bTC) ELISA Kit |
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| MBS2021662-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 2390 |
Mouse Betacellulin (bTC) ELISA Kit |
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| MBS2021662-96StripWells | MyBiosource | 96-Strip-Wells | EUR 590 |
Mouse Betacellulin (bTC) ELISA Kit |
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| MBS452107-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 4225 |
Mouse Betacellulin (bTC) ELISA Kit |
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| MBS452107-48StripWells | MyBiosource | 48-Strip-Wells | EUR 375 |
Mouse Betacellulin (bTC) ELISA Kit |
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| MBS452107-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 2175 |
Mouse Betacellulin (bTC) ELISA Kit |
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| MBS452107-96StripWells | MyBiosource | 96-Strip-Wells | EUR 490 |
Mouse Betacellulin (bTC) ELISA Kit |
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| MBS2701249-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 2475 |
Mouse Betacellulin (bTC) ELISA Kit |
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| MBS2701249-24StripWells | MyBiosource | 24-Strip-Wells | EUR 230 |
Mouse Betacellulin (bTC) ELISA Kit |
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| MBS2701249-48StripWells | MyBiosource | 48-Strip-Wells | EUR 290 |
Mouse Betacellulin (bTC) ELISA Kit |
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| MBS2701249-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 1290 |
Mouse Betacellulin (bTC) ELISA Kit |
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| MBS2701249-96StripWells | MyBiosource | 96-Strip-Wells | EUR 355 |
Mouse Betacellulin (BTC) ELISA Kit |
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| MBS2610691-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 3290 |
Mouse Betacellulin (BTC) ELISA Kit |
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| MBS2610691-48StripWells | MyBiosource | 48-Strip-Wells | EUR 295 |
Mouse Betacellulin (BTC) ELISA Kit |
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| MBS2610691-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 1800 |
Mouse Betacellulin (BTC) ELISA Kit |
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| MBS2610691-96StripWells | MyBiosource | 96-Strip-Wells | EUR 450 |
Mouse Betacellulin (bTC) ELISA Kit |
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| MBS457992-10x96Tests | MyBiosource | 10x96Tests | EUR 4405 |
Mouse Betacellulin (bTC) ELISA Kit |
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| MBS457992-48Tests | MyBiosource | 48Tests | EUR 385 |
Mouse Betacellulin (bTC) ELISA Kit |
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| MBS457992-5x96Tests | MyBiosource | 5x96Tests | EUR 2260 |
Mouse Betacellulin (bTC) ELISA Kit |
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| MBS457992-96Tests | MyBiosource | 96Tests | EUR 510 |
Mouse BTC -Betacellulin- CLIA Kit |
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| E-CL-M0133-24Tests | Elabscience Biotech | 24 Tests | EUR 180 |
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Description: Sandwich |
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Mouse BTC -Betacellulin- CLIA Kit |
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| E-CL-M0133-48Tests | Elabscience Biotech | 48 Tests | EUR 546 |
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Description: Sandwich |
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Mouse BTC -Betacellulin- CLIA Kit |
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| E-CL-M0133-96Tests | Elabscience Biotech | 96 Tests | EUR 682 |
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Description: Sandwich |
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Mouse BTC -Betacellulin- CLIA Kit |
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| E-CL-M0133-96Tests10 | Elabscience Biotech | 96 Tests *10 | EUR 6820 |
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Description: Sandwich |
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Mouse BTC -Betacellulin- CLIA Kit |
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| E-CL-M0133-96Tests5 | Elabscience Biotech | 96 Tests *5 | EUR 3410 |
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Description: Sandwich |
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Mouse bTC(Betacellulin) ELISA Kit |
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| ELK1236-48T | ELK Biotech | 48T | Ask for price |
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Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse bTC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse bTC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse bTC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse bTC in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Mouse bTC(Betacellulin) ELISA Kit |
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| ELK1236-96T | ELK Biotech | 96T | Ask for price |
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Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse bTC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse bTC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse bTC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse bTC in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Mouse bTC (Betacellulin) ELISA Kit |
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| MBS8800238-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 3130 |
Mouse bTC (Betacellulin) ELISA Kit |
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| MBS8800238-48StripWells | MyBiosource | 48-Strip-Wells | EUR 315 |
Mouse bTC (Betacellulin) ELISA Kit |
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| MBS8800238-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 1710 |
Mouse bTC (Betacellulin) ELISA Kit |
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| MBS8800238-96StripWells | MyBiosource | 96-Strip-Wells | EUR 385 |
Mouse BTC (Betacellulin) ELISA Kit |
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| MBS2511826-10x96Tests | MyBiosource | 10x96Tests | EUR 4060 |
Mouse BTC (Betacellulin) ELISA Kit |
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| MBS2511826-24Tests | MyBiosource | 24Tests | EUR 265 |
Mouse BTC (Betacellulin) ELISA Kit |
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| MBS2511826-48Tests | MyBiosource | 48Tests | EUR 450 |
Mouse BTC (Betacellulin) ELISA Kit |
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| MBS2511826-5x96Tests | MyBiosource | 5x96Tests | EUR 2265 |
Mouse BTC (Betacellulin) ELISA Kit |
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| MBS2511826-96Tests | MyBiosource | 96Tests | EUR 535 |
Mouse BTC(Betacellulin) ELISA Kit |
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| RE2928M-48wellsplate | Reed Biotech | 48-wells plate | EUR 126 |
Mouse BTC(Betacellulin) ELISA Kit |
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| RE2928M-96wellsplate | Reed Biotech | 96-wells plate | EUR 180 |
Mouse Betacellulin Recombinant Protein |
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| M10-130 | ReliaTech | 20 µg | EUR 196.35 |
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Description: Betacellulin (BTC or beta-TC) is a member of the EGF family of cytokines which includes EGF, TGFα, amphiregulin, HB-EGF, epiregulin, tomoregulin and the neuregulins. All EGF famiy members are synthesized as type I transmembrane precursor proteins containing one or more EGF-like domains in their extracellular region. BTC, a heparin-binding protein, was originally isolated from the conditioned media of mouse pancreatic beta tumor cells as a 32 kDa glycoprotien. The mouse BTC cDNA encodes a 177 amino acid (aa) residue precursor with a 31 aa signal peptide, an 87 aa residue extracellular region containing one EGF-like domain, a 21 aa transmembrane domain and a 38 aa cytoplasmic domain. Soluble BTC is released from the transmembrane precursor by proteolytic processing. Mouse BTC shares 93% and 79% aa sequence identity with rat and human BTC, respectively. The mouse BTC gene is tightly linked to that of amphiregulin on mouse chromosome 5. BTC is expressed in most tissues including kidney, uterus, liver and pancreas. It is also present in body fluids including serum, milk and colostrum. BTC promotes pancreatic betacell growth and differentiation and is a potent mitogen for retinal pigment epithelial cells, vascular smooth muscle cells and fibroblasts. The effects of BTC is mediated by binding to ErbB1 and ErbB4 homodimers as well as ErbB heterodimers. Recombinant murine Betacellulin is a 9.0 kDa monomeric protein, containing 80 amino residues, which comprises the mature EGF homologous portion of the Betacellulin protein. |
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Mouse Betacellulin Recombinant Protein |
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| M10-130S | ReliaTech | 5 µg | EUR 92.4 |
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Description: Betacellulin (BTC or beta-TC) is a member of the EGF family of cytokines which includes EGF, TGFα, amphiregulin, HB-EGF, epiregulin, tomoregulin and the neuregulins. All EGF famiy members are synthesized as type I transmembrane precursor proteins containing one or more EGF-like domains in their extracellular region. BTC, a heparin-binding protein, was originally isolated from the conditioned media of mouse pancreatic beta tumor cells as a 32 kDa glycoprotien. The mouse BTC cDNA encodes a 177 amino acid (aa) residue precursor with a 31 aa signal peptide, an 87 aa residue extracellular region containing one EGF-like domain, a 21 aa transmembrane domain and a 38 aa cytoplasmic domain. Soluble BTC is released from the transmembrane precursor by proteolytic processing. Mouse BTC shares 93% and 79% aa sequence identity with rat and human BTC, respectively. The mouse BTC gene is tightly linked to that of amphiregulin on mouse chromosome 5. BTC is expressed in most tissues including kidney, uterus, liver and pancreas. It is also present in body fluids including serum, milk and colostrum. BTC promotes pancreatic betacell growth and differentiation and is a potent mitogen for retinal pigment epithelial cells, vascular smooth muscle cells and fibroblasts. The effects of BTC is mediated by binding to ErbB1 and ErbB4 homodimers as well as ErbB heterodimers. Recombinant murine Betacellulin is a 9.0 kDa monomeric protein, containing 80 amino residues, which comprises the mature EGF homologous portion of the Betacellulin protein. |
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Recombinant Mouse Betacellulin protein |
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| CD00612-5ug | Jiaxing Korain Biotech Ltd (BT Labs) | 5ug | EUR 176 |
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Description: Betacellulin (BTC) encoded by the BTC gene located on the chromosome 4, is a member of the EGF family that also includes EGF, TGF-alpha, Amphiregulin, HB-EGF, Epiregulin, Tomoregulin and the Neuregulins. BTC is expressed in most tissues including kidney, uterus, liver and pancreas. It is also present in body fluids, including serum, milk, and colostrum. Mouse BTC is expressed as a 178-amino acid precursor and the amino acid sequence of the mature form is 80 % identical with human BTC. Both human and mouse BTC exhibit significant overall similarity with other members of the EGF family. Recombinant Mouse Betacellulin is a heparin binding protein containing 80 amino acids residues. |
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Rat Monoclonal anti-mouse Betacellulin |
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| mAP-0154 | Angio Proteomie | 100ug | EUR 250 |
Betacellulin (bTC) Polyclonal Antibody (Mouse) |
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| 4-PAA112Mu01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Betacellulin (bTC) |
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Betacellulin (HEK293-expressed), Mouse |
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| HY-P7142 | MedChemExpress | 50ug | EUR 639.6 |
CLIA kit for Mouse BTC (Betacellulin) |
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| E-CL-M0133 | Elabscience Biotech | 1 plate of 96 wells | EUR 700.8 |
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Description: A sandwich CLIA kit for quantitative measurement of Mouse BTC (Betacellulin) in samples from Serum, Plasma, Cell supernatant |
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ELISA kit for Mouse BTC (Betacellulin) |
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| E-EL-M0181 | Elabscience Biotech | 1 plate of 96 wells | EUR 640.8 |
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Description: A sandwich ELISA kit for quantitative measurement of Mouse BTC (Betacellulin) in samples from Serum, Plasma, Cell supernatant |
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ELISA kit for Mouse bTC (Betacellulin) |
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| ELK1236 | ELK Biotech | 1 plate of 96 wells | EUR 446.4 |
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Description: A sandwich ELISA kit for detection of Betacellulin from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
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Anti-BTC/Betacellulin Antibody |
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| MBS2400715-005mg | MyBiosource | 0.05mg | EUR 595 |
Anti-BTC/Betacellulin Antibody |
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| MBS2400715-5x005mg | MyBiosource | 5x0.05mg | EUR 2500 |
Betacellulin (bTC) Polyclonal Antibody (Mouse), PE |
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| 4-PAA112Mu01-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Betacellulin (bTC). This antibody is labeled with PE. |
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Anti-Human Betacellulin Antibody |
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| 101-M143 | ReliaTech | 100 µg | EUR 399 |
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Description: Betacellulin (BTC) is a new member of the EGF family of cytokines that is comprised of at least ten proteins including EGF, TGF αlpha, amphiregulin, HBEGF, and the various heregulins. All of these cytokines are synthesized as transmembrane precursors and are characterized by the presence of one or more EGF structural units in their extracellular domain. The soluble forms of these cytokines are released by proteolytic cleavage. BTC, a heparin binding protein, was originally isolated from the conditioned media of mouse pancreatic beta tumor cells as a 32 kDa glycoprotein composed of 80 amino acid residues. The cDNA encoding human BTC was cloned from a human breast adenocarcinoma cell line (MCF7) cDNA library. Human and mouse cDNAs encode BTC precursor proteins of 178 and 177 amino acid residues, respectively. At the amino acid sequence level, human BTC precursor protein exhibits 79% identity with that of the mouse BTC precursor. In a mouse cell line transfected with human BTC cDNA, three forms of soluble human BTC have been detected: the glycosylated, intact BTC composed of 80 amino acid residues, a truncated molecule lacking 12 amino acid residues from the amino terminus, and a second truncated molecule lacking 30 amino acid residues from the amino terminus. The biological activities of these BTC forms were shown to be identical. BTC can bind to the EGF receptor and is a potent mitogen for Balb/c 3T3 fibroblasts, retinal pigment epithelial cells and vascular smooth muscle cells. |
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Anti-Human Betacellulin Antibody |
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| 101-M230 | ReliaTech | 100 µg | EUR 399 |
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Description: Betacellulin (BTC) is a new member of the EGF family of cytokines that is comprised of at least ten proteins including EGF, TGF αlpha, amphiregulin, HBEGF, and the various heregulins. All of these cytokines are synthesized as transmembrane precursors and are characterized by the presence of one or more EGF structural units in their extracellular domain. The soluble forms of these cytokines are released by proteolytic cleavage. BTC, a heparin binding protein, was originally isolated from the conditioned media of mouse pancreatic beta tumor cells as a 32 kDa glycoprotein composed of 80 amino acid residues. The cDNA encoding human BTC was cloned from a human breast adenocarcinoma cell line (MCF7) cDNA library. Human and mouse cDNAs encode BTC precursor proteins of 178 and 177 amino acid residues, respectively. At the amino acid sequence level, human BTC precursor protein exhibits 79% identity with that of the mouse BTC precursor. In a mouse cell line transfected with human BTC cDNA, three forms of soluble human BTC have been detected: the glycosylated, intact BTC composed of 80 amino acid residues, a truncated molecule lacking 12 amino acid residues from the amino terminus, and a second truncated molecule lacking 30 amino acid residues from the amino terminus. The biological activities of these BTC forms were shown to be identical. BTC can bind to the EGF receptor and is a potent mitogen for Balb/c 3T3 fibroblasts, retinal pigment epithelial cells and vascular smooth muscle cells. |
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Anti-Human Betacellulin Antibody |
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| 102-P222 | ReliaTech | 100 µg | EUR 245.7 |
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Description: Betacellulin is an EGF-related polypeptide growth factor that signals through the EGF receptor. It is produced in several tissues, including the pancreas, small intestine, and in certain tumor cells. Betacellulin is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. Human Betacellulin is initially synthesized as a glycosylated 32.0 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce the mature sequence. Recombinant human Betacellulin is a 9.0 kDa monomeric protein, containing 80 amino residues, which comprises the mature EGF homologous portion of the Betacellulin protein. |
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Mouse Betacellulin/BTC PicoKine ELISA Kit |
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| EK1193 | BosterBio | 96 wells | EUR 510 |
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Description: For quantitative detection of mouse BTC in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
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Mouse Betacellulin/BTC PicoKine ELISA Kit |
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| MBS177297-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 2755 |
Mouse Betacellulin/BTC PicoKine ELISA Kit |
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| MBS177297-96StripWells | MyBiosource | 96-Strip-Wells | EUR 600 |
Betacellulin (bTC) Polyclonal Antibody (Mouse), APC |
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| 4-PAA112Mu01-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Betacellulin (bTC). This antibody is labeled with APC. |
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Betacellulin (bTC) Polyclonal Antibody (Mouse), Cy3 |
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| 4-PAA112Mu01-Cy3 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Betacellulin (bTC). This antibody is labeled with Cy3. |
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Betacellulin (bTC) Polyclonal Antibody (Mouse), HRP |
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| 4-PAA112Mu01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Betacellulin (bTC). This antibody is labeled with HRP. |
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Betacellulin (bTC) Polyclonal Antibody (Mouse), FITC |
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| 4-PAA112Mu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Betacellulin (bTC). This antibody is labeled with FITC. |
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BTC Betacellulin Mouse Recombinant Protein |
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| PROTQ05928 | BosterBio | Regular: 20ug | EUR 380.4 |
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Description: BTC Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 80 amino acids and having a molecular mass of 9.0kDa. ;The BTC is purified by proprietary chromatographic techniques. |
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Recombinant Mouse Betacellulin/BTC (N-6His) |
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| AP75333 | SAB | 1mg | EUR 3209 |
Recombinant Mouse Betacellulin/BTC (N-6His) |
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| C747-10ug | Novoprotein | 10ug | EUR 187.2 |
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Description: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4. |
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Recombinant Mouse Betacellulin/BTC (N-6His) |
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| C747-1mg | Novoprotein | 1mg | EUR 2739.6 |
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Description: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4. |
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Recombinant Mouse Betacellulin/BTC (N-6His) |
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| C747-500ug | Novoprotein | 500ug | EUR 1935.6 |
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Description: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4. |
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Recombinant Mouse Betacellulin/BTC (N-6His) |
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| C747-50ug | Novoprotein | 50ug | EUR 442.8 |
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Description: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4. |
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Mouse Monoclonal ant-Human Betacellulin (BTC) |
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| hAP-0110 | Angio Proteomie | 100ug | EUR 250 |
Mouse Monoclonal ant-Human Betacellulin (BTC) |
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| hAP-0110A | Angio Proteomie | 100ug | EUR 250 |
Mouse Betacellulin/BTC(Beta Cellulin) ELISA Kit |
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| EM0021 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 4.688pg/ml |
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Betacellulin/BTC ELISA Kit (Mouse) (OKBB00530) |
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| OKBB00530 | Aviva Systems Biology | 96 Wells | EUR 606 |
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Description: Description of target: Betacellulin, also known as BTC is a protein that in humans is encoded by the BTC gene. This gene is mapped to 4q13.3. The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. It is the growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. This protein is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.;Species reactivity: Mouse;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: <= 10 pg/mL |
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Betacellulin antibody |
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| 70R-BR013 | Fitzgerald | 50 ug | EUR 312 |
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Description: Affinity purified Rabbit polyclonal Betacellulin antibody |
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Betacellulin antibody |
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| MBS535912-005mg | MyBiosource | 0.05mg | EUR 465 |
Betacellulin antibody |
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| MBS535912-5x005mg | MyBiosource | 5x0.05mg | EUR 1945 |
Mouse Betacellulin HEK293 Overexpression Lysate |
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| MBS8116554-03mg | MyBiosource | 0.3mg | EUR 280 |
Mouse Betacellulin HEK293 Overexpression Lysate |
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| MBS8116554-5x03mg | MyBiosource | 5x0.3mg | EUR 1205 |
Betacellulin (BTC) |
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| THP-0274 | Creative BioMart | 1 vial | EUR 3198.4 |
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Description: Protein |
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Mouse Betacellulin Recombinant Protein Lyophilized |
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| IMSBTCRLY5UG | Innovative research | each | EUR 341 |
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Description: Mouse Betacellulin Recombinant Protein Lyophilized |
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Mouse Betacellulin Recombinant Protein Lyophilized |
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| MBS8433954-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Betacellulin (bTC) Polyclonal Antibody (Mouse), APC-Cy7 |
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| 4-PAA112Mu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Betacellulin (bTC). This antibody is labeled with APC-Cy7. |
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Mouse Betacellulin Chemiluminescent Immunoassay Kit |
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| IMSBTCKTC | Innovative research | each | EUR 545 |
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Description: Mouse Betacellulin Chemiluminescent Immunoassay Kit |
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Mouse Betacellulin Flow Cytometry Assay - Group 3 |
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| MX78121 | Antigenix America | 96 Tests | EUR 270 |
Betacellulin, Human |
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| HY-P7005 | MedChemExpress | 10ug | EUR 253.2 |
Betacellulin, human |
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| RC218-23 | Bio Basic | 5ug | EUR 125.26 |
Human Betacellulin |
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| MBS691789-0005mg | MyBiosource | 0.005mg | EUR 300 |
Human Betacellulin |
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| MBS691789-002mg | MyBiosource | 0.02mg | EUR 450 |
Human Betacellulin |
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| MBS691789-5x002mg | MyBiosource | 5x0.02mg | EUR 1725 |
Betacellulin (bTC) Polyclonal Antibody (Mouse), Biotinylated |
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| 4-PAA112Mu01-Biotin | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Betacellulin (bTC). This antibody is labeled with Biotin. |
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Bovine Betacellulin |
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| ARU020 | GroPep | 20 µg | EUR 303.6 |
Bovine Betacellulin |
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| ARU100 | GroPep | 100 µg | EUR 415.2 |
Recombinant Mouse Betacellulin/BTC Protein (His Tag) |
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| PKSM040967-10ug | Elabscience Biotech | 10ug | EUR 129 |
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Description: Mouse |
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Recombinant Mouse Betacellulin/BTC Protein (His Tag) |
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| PKSM040967-50ug | Elabscience Biotech | 50ug | EUR 375 |
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Description: Mouse |
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Betacellulin (BTC) Antibody |
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| 20-abx111218 | Abbexa |
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Betacellulin (bTC) Antibody |
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| 20-abx100739 | Abbexa |
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Betacellulin (bTC) Antibody |
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| 20-abx100740 | Abbexa |
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Betacellulin (BTC) Antibody |
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| abx230883-100ug | Abbexa | 100 ug | EUR 577.2 |
Betacellulin (bTC) Antibody |
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| 20-abx171411 | Abbexa |
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Betacellulin (bTC) Antibody |
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| 20-abx175568 | Abbexa |
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Betacellulin (bTC) Antibody |
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| 20-abx175569 | Abbexa |
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Betacellulin (bTC) Antibody |
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| 20-abx175570 | Abbexa |
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Betacellulin (bTC) Antibody |
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| 20-abx175571 | Abbexa |
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BTC Antibody / Betacellulin |
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| F54607-0.08ML | NSJ Bioreagents | 0.08 ml | EUR 140.25 |
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Description: Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. [UniProt] |
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BTC Antibody / Betacellulin |
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| F54607-0.4ML | NSJ Bioreagents | 0.4 ml | EUR 322.15 |
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Description: Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. [UniProt] |
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Betacellulin Antibody / BTC |
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| RQ4417 | NSJ Bioreagents | 100 ug | EUR 356.15 |
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Description: Betacellulin is a protein that in humans is encoded by the BTC gene located on chromosome 4 at locus 4q13-q21. This gene encodes a member of the epidermal growth factor (EGF) family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the secreted growth factor. A secreted form and a membrane-anchored form of this protein bind to multiple different EGF receptors. This protein promotes pancreatic cell proliferation and insulin secretion, as well as retinal vascular permeability. Mutations in this gene may be associated with type 2 diabetes in human patients. |
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BTC Antibody / Betacellulin |
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| RQ4539 | NSJ Bioreagents | 100ug | EUR 356.15 |
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Description: Betacellulin is a protein that in humans is encoded by the BTC gene located on chromosome 4 at locus 4q13-q21. This gene encodes a member of the epidermal growth factor (EGF) family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the secreted growth factor. A secreted form and a membrane-anchored form of this protein bind to multiple different EGF receptors. This protein promotes pancreatic cell proliferation and insulin secretion, as well as retinal vascular permeability. Mutations in this gene may be associated with type 2 diabetes in human patients. |
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Betacellulin (bTC) Antibody |
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| abx171411-100l | Abbexa | 100 µl | EUR 712.5 |
Betacellulin (bTC) Antibody |
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| abx171411-1ml | Abbexa | 1 ml | Ask for price |
Betacellulin (bTC) Antibody |
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| abx171411-200l | Abbexa | 200 µl | Ask for price |
Betacellulin (bTC) Antibody |
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| abx175570-1096tests | Abbexa | 10 × 96 tests | Ask for price |
Betacellulin (bTC) Antibody |
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| abx175570-596tests | Abbexa | 5 × 96 tests | Ask for price |
Betacellulin (bTC) Antibody |
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| abx175570-96tests | Abbexa | 96 tests | EUR 725 |
Betacellulin (BTC) Antibody |
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| abx111218-100l | Abbexa | 100 µl | EUR 612.5 |
A easy, straightforward and low cost methodology that may be utilized to supply a lot of genetically-modified BM-EPCs was established, which can facilitate the research of GTE-EPC therapeutic efficacy in pig MI mannequin.