Mutations within the fukutin-related protein (FKRP) gene end in a broad spectrum of muscular dystrophy (MD) phenotypes, together with the extreme Walker-Warburg syndrome (WWS). Right here, we develop a gene-editing method that replaces your complete mutant open studying body with the wild-type sequence to universally right all FKRP mutations.
We apply this method to right FKRP mutations in induced pluripotent stem (iPS) cells derived from sufferers displaying broad medical severity. Our findings present rescue of useful α-dystroglycan (α-DG) glycosylation in gene-edited WWS iPS cell-derived myotubes. Transplantation of gene-corrected myogenic progenitors within the FKRPP448L-NSG mouse mannequin offers rise to myofiber and satellite tv for pc cell engraftment and, importantly, restoration of α-DG useful glycosylation in vivo. These findings counsel the potential feasibility of utilizing CRISPR-Cas9 expertise together with patient-specific iPS cells for the long run growth of autologous cell transplantation for FKRP-associated MDs.
An replace on Alzheimer’s illness: Immunotherapeutic brokers, stem cell remedy and gene enhancing
Alzheimer’s illness is a persistent way of life ailment whose incidence has come to gentle with the rising life expectancy because of higher healthcare. The affected person burden for AD is about to double by the yr 2060 and development in analysis is of utmost significance to fight this downside. AD is characterised by the pathological hallmarks of amyloid plaques and neurofibrillary tangles.
The illness has been implicated to have a genetic predisposition. The present therapy methods are at finest ameliorative in nature and provide no substantive remedy. Immunotherapeutic approaches employed have proven few therapeutic advantages however the accelerated approval of aducanumab by the US-FDA reveals medical profit benefit. As well as, newer therapeutic approaches are the necessity of the hour. This evaluation goals to focus on the pathology of the illness, adopted by an perception into newer approaches like stem cell remedy and gene enhancing, specializing in potential CRISPR mediated targets.
An outline of cell and gene remedy growth in China
China, the primary nation worldwide to approve a gene remedy in 2003, nearly misplaced the benefit for a head begin in cell and gene remedy (CGT) growth because of a scarcity of clear and strict regulatory frameworks. The speedy developments of CGTs’ growth worldwide in addition to their therapeutic potential have triggered the federal government to conduct a spate of regulatory reforms as a way to promote normative growth of CGTs in China.
Inspired by coverage assist, the outstanding progress for CGTs in China has been noticed over the previous few years, thereby catapulting China again into the forefront of CGTs worldwide. This manuscript goals to offer an outline of regulatory reforms, the present growth panorama of CGTs, in addition to key contributors and challenges for CGT growth in China.
Gene Remedy for Sickle Cell Illness – Transferring from the Bench to the Bedside
Gene remedy as a possible remedy for sickle cell illness (SCD) has lengthy been pursued provided that this hemoglobin dysfunction outcomes from a single level mutation. Advances in genomic sequencing, elevated understanding of hemoglobin regulation and discoveries of molecular instruments for genome modification of hematopoietic stem cells have made gene remedy for SCD potential. Gene addition methods utilizing gene switch vectors have been optimized over the previous couple of many years to allow expression of regular or anti-sickling globins as methods to ameliorate SCD.
Many hurdles needed to be addressed previous to medical translation together with assortment of ample stem cells for gene-modification, rising expression of transferred genes to a therapeutic degree and conditioning sufferers in a protected method that enabled enough engraftment of gene-modified cells. The invention of genome editors that make exact modifications has additional superior the protection and efficacy of gene remedy and a speedy motion to medical trial has undoubtedly been supported by classes realized from optimizing gene addition methods.
Present gene therapies being examined in medical trial require important infrastructure and experience given the wants to reap cells from and administer chemotherapy to sufferers who usually have important organ dysfunction and that gene-modification takes place ex vivo in specialised amenities. For these therapies to appreciate their full potential they might have to be transportable, protected and environment friendly making an in-vivo primarily based method engaging. Moreover, enough assets for SCD screening and entry to standardized care are critically necessary for gene remedy to be a viable therapy possibility for SCD.
Gene and cell remedy for the nucleus basalis of Meynert with NGF in Alzheimer’s illness
There may be at present no efficient therapy for the most typical of the dementia issues, Alzheimer’s illness (AD). It has been recognized for many years that the central cholinergic system is necessary for reminiscence. The cholinergic neurons within the basal forebrain with its cortical and hippocampal projections degenerate in AD and thus contribute to the cognitive decline attribute of AD.
This data led to the event of the at present permitted therapy for AD, with inhibitors of acetylcholine-esterase concentrating on the cholinergic system with useful however gentle results. Lately, different approaches to affect the degenerating cholinergic system in AD specializing in nerve development issue (NGF) have been undertaken. NGF is required for the survival and performance of the basal forebrain cholinergic neurons, an important being the nucleus basalis of Meynert (nbM).
CFDA SE cell prolifeRation and tracer detection kit |
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| MBS8579509-2000Assays | MyBiosource | 2000Assays | EUR 400 |
CFDA SE cell prolifeRation and tracer detection kit |
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| MBS8579509-5x2000Assays | MyBiosource | 5x2000Assays | EUR 1810 |
FluoGreen Tracer |
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| SF41000 | Neuromics | 100 ul | EUR 292.05 |
Tau tracer 1 |
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| HY-134879 | MedChemExpress | Get quote | Ask for price |
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Description: Tau tracer 1 is a Tau tracer used for imaging Tau protein aggregates. Tau tracer 1 can be used to diagnose neurodegenerative diseases[1]. |
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Tau tracer 2 |
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| HY-134880 | MedChemExpress | 10 mg | EUR 216.45 |
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Description: Tau tracer 2 (Pl-2620) is a Tau tracer used for imaging Tau protein aggregates. Tau tracer 2 can be used to diagnose neurodegenerative diseases[1]. |
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Tau tracer 1 |
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| MBS5800094-5mg | MyBiosource | 5(mg | EUR 915 |
Tau tracer 1 |
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| MBS5800094-5x5mg | MyBiosource | 5x5(mg | EUR 3970 |
Tau tracer 2 |
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| MBS5798041-50mg | MyBiosource | 50(mg | EUR 960 |
Tau tracer 2 |
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| MBS5798041-5x50mg | MyBiosource | 5x50(mg | EUR 4180 |
Tau tracer 2 |
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| T37051-10mg | TargetMol Chemicals | 10mg | Ask for price |
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Description: Tau tracer 2 |
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Tau tracer 2 |
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| T37051-1g | TargetMol Chemicals | 1g | Ask for price |
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Description: Tau tracer 2 |
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Tau tracer 2 |
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| T37051-1mg | TargetMol Chemicals | 1mg | Ask for price |
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Description: Tau tracer 2 |
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Tau tracer 2 |
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| T37051-50mg | TargetMol Chemicals | 50mg | Ask for price |
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Description: Tau tracer 2 |
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Tau tracer 2 |
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| T37051-5mg | TargetMol Chemicals | 5mg | Ask for price |
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Description: Tau tracer 2 |
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Tau tracer 1 |
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| T39660-10mg | TargetMol Chemicals | 10mg | Ask for price |
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Description: Tau tracer 1 |
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Tau tracer 1 |
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| T39660-1g | TargetMol Chemicals | 1g | Ask for price |
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Description: Tau tracer 1 |
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Tau tracer 1 |
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| T39660-1mg | TargetMol Chemicals | 1mg | Ask for price |
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Description: Tau tracer 1 |
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Tau tracer 1 |
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| T39660-50mg | TargetMol Chemicals | 50mg | Ask for price |
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Description: Tau tracer 1 |
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Tau tracer 1 |
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| T39660-5mg | TargetMol Chemicals | 5mg | Ask for price |
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Description: Tau tracer 1 |
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JAK2 JH2 Tracer |
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| HY-102055 | MedChemExpress | 50mg | EUR 2705.67 |
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Description: JAK2 JH2 Tracer (Tracer 5) is a fluorescent probe for JAK2 JH2 domain, with a Kd of value 0.2 μM[1][2]. |
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JAK2 JH2 Tracer |
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| MBS3844504-10mg | MyBiosource | 10mg | EUR 855 |
JAK2 JH2 Tracer |
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| MBS3844504-1mg | MyBiosource | 1mg | EUR 225 |
JAK2 JH2 Tracer |
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| MBS3844504-50mg | MyBiosource | 50mg | EUR 2450 |
JAK2 JH2 Tracer |
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| MBS3844504-5mg | MyBiosource | 5mg | EUR 525 |
JAK2 JH2 Tracer |
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| MBS3844504-5x50mg | MyBiosource | 5x50mg | EUR 11020 |
JAK2 JH2 Tracer |
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| MBS5755856-50mg | MyBiosource | 50mg | EUR 2840 |
JAK2 JH2 Tracer |
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| MBS5755856-5x50mg | MyBiosource | 5x50mg | EUR 12620 |
JAK2 JH2 Tracer |
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| T19017-10mg | TargetMol Chemicals | 10mg | Ask for price |
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Description: JAK2 JH2 Tracer |
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JAK2 JH2 Tracer |
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| T19017-1g | TargetMol Chemicals | 1g | Ask for price |
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Description: JAK2 JH2 Tracer |
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JAK2 JH2 Tracer |
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| T19017-1mg | TargetMol Chemicals | 1mg | Ask for price |
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Description: JAK2 JH2 Tracer |
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JAK2 JH2 Tracer |
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| T19017-50mg | TargetMol Chemicals | 50mg | Ask for price |
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Description: JAK2 JH2 Tracer |
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JAK2 JH2 Tracer |
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| T19017-5mg | TargetMol Chemicals | 5mg | Ask for price |
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Description: JAK2 JH2 Tracer |
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ROS tracer precursor |
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| HY-126712 | MedChemExpress | Get quote | Ask for price |
|
Description: ROS tracer precursor is the precursor of [18F]ROStrace for the synthesis of ROStrace, which can be used for disease diagnosis[1]. |
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ROS tracer precursor |
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| MBS5772039-5mg | MyBiosource | 5mg | EUR 915 |
ROS tracer precursor |
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| MBS5772039-5x5mg | MyBiosource | 5x5mg | EUR 3970 |
ROS tracer precursor |
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| T19550-10mg | TargetMol Chemicals | 10mg | Ask for price |
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Description: ROS tracer precursor |
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ROS tracer precursor |
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| T19550-1g | TargetMol Chemicals | 1g | Ask for price |
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Description: ROS tracer precursor |
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ROS tracer precursor |
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| T19550-1mg | TargetMol Chemicals | 1mg | Ask for price |
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Description: ROS tracer precursor |
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ROS tracer precursor |
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| T19550-50mg | TargetMol Chemicals | 50mg | Ask for price |
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Description: ROS tracer precursor |
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ROS tracer precursor |
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| T19550-5mg | TargetMol Chemicals | 5mg | Ask for price |
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Description: ROS tracer precursor |
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CytoFixâ„¢ BCECF, AM *Optimized for long term cellular pH tracking* |
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| 21200-1mg | AAT Bioquest | 1 mg | EUR 318 |
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Description: Intracellular pH plays an important modulating role in many cellular events, including cell growth, calcium regulation, enzymatic activity, receptor-mediated signal transduction, ion transport, endocytosis, chemotaxis, cell adhesion and other cellular processes. |
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Long-Term Cell Tracing Reagent CMAC （Blue) |
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| EGY0171 | EnoGene | 100µg | EUR 92 |
Long-Term Cell Tracing Reagent CMAC （Blue) |
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| EGY0172 | EnoGene | 5x100µg | EUR 340 |
Long-Term Cell Tracing Reagent CMAC (Blue) |
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| MBS8579546-01mg | MyBiosource | 0.1mg | EUR 185 |
Long-Term Cell Tracing Reagent CMAC (Blue) |
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| MBS8579546-5x01mg | MyBiosource | 5x0.1mg | EUR 420 |
Long-Term Cell Tracing Reagent CMFDA（Green） |
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| EGY0181 | EnoGene | 100µg | EUR 220 |
Long-Term Cell Tracing Reagent CMFDA（Green） |
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| EGY0182 | EnoGene | 5x100µg | EUR 500 |
Long-Term Cell Tracing Reagent CMFDA (Green) |
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| MBS8579547-01mg | MyBiosource | 0.1mg | EUR 300 |
Long-Term Cell Tracing Reagent CMFDA (Green) |
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| MBS8579547-5x01mg | MyBiosource | 5x0.1mg | EUR 580 |
IAA tracer (alkaline phospahatase conjugated) |
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| AS09-517 | Agrisera AB | 1000 assays | EUR 208 |
ABA tracer (alkaline phospahatase conjugated) |
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| AS10-726 | Agrisera AB | 1000 assays | EUR 230 |
Long-Term Cell Tracing Reagent CDCFDA, SE （Green） |
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| EGY0161 | EnoGene | 100 µg | EUR 160 |
Long-Term Cell Tracing Reagent CDCFDA, SE （Green） |
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| EGY0162 | EnoGene | 5 x 100 µg | EUR 340 |
Long-Term Cell Tracing Reagent CDCFDA, SE (Green) |
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| MBS8579545-01mg | MyBiosource | 0.1mg | EUR 245 |
Long-Term Cell Tracing Reagent CDCFDA, SE (Green) |
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| MBS8579545-5x01mg | MyBiosource | 5x0.1mg | EUR 420 |
NIR-FLIVO 690 Tracer In vivo Assay |
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| MBS258410-20Tests | MyBiosource | 20Tests | EUR 535 |
NIR-FLIVO 690 Tracer In vivo Assay |
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| MBS258410-5x20Tests | MyBiosource | 5x20Tests | EUR 2460 |
NIR-FLIVO 747 Tracer In vivo Assay |
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| MBS258412-20Tests | MyBiosource | 20Tests | EUR 535 |
NIR-FLIVO 747 Tracer In vivo Assay |
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| MBS258412-5x20Tests | MyBiosource | 5x20Tests | EUR 2460 |
Cis-zeatin tracer (alkaline phospahatase conjugated) |
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| AS16-4092 | Agrisera AB | 1000 assays | EUR 230 |
Trans-zeatin tracer (alkaline phospahatase conjugated) |
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| AS16-4090 | Agrisera AB | 1000 assays | EUR 230 |
NIR-FLIVO® 690 Tracer In vivo Assay |
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| 9112 | Immunochemistry | 20 Tests | EUR 438 |
NIR-FLIVO® 747 Tracer In vivo Assay |
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| 9114 | Immunochemistry | 20 Tests | EUR 438 |
CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Colorimetric |
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| CBA-135 | Cell Biolabs | 96 assays | EUR 596 |
CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Colorimetric |
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| CBA-135-5 | Cell Biolabs | 5 x 96 assays | EUR 2592 |
CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Fluorometric |
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| CBA-140 | Cell Biolabs | 96 assays | EUR 624 |
CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Fluorometric |
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| CBA-140-5 | Cell Biolabs | 5 x 96 assays | EUR 2700 |
CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric, Trial Size |
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| CBA-135-T | Cell Biolabs | 24 assays | EUR 518.4 |
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Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis. |
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N6-isopentenyladenosine tracer (alkaline phospahatase conjugated) |
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| AS17-4132 | Agrisera AB | 1000 assays | EUR 208 |
HRP conjugated mAb anti-Human TNF-alpha (Tracer) |
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| MBS592166-02mL | MyBiosource | 0.2mL | EUR 580 |
HRP conjugated mAb anti-Human TNF-alpha (Tracer) |
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| MBS592166-5x02mL | MyBiosource | 5x0.2mL | EUR 2355 |
CytoSelect 96-Well Cell Transformation Assay (Cell Recovery Compatible, Fluorometric), Trial Size |
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| CBA-140-T | Cell Biolabs | 24 assays | EUR 547.2 |
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Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis. |
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Biotin conjugated mAb anti-Human TNF-alpha (Tracer) |
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| MBS592389-01mL | MyBiosource | 0.1mL | EUR 580 |
Biotin conjugated mAb anti-Human TNF-alpha (Tracer) |
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| MBS592389-5x01mL | MyBiosource | 5x0.1mL | EUR 2355 |
CytoSelect™ 96-well Cell Transformation Assay |
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| CBA-130 | Cell Biolabs | 96 assays | EUR 532 |
CytoSelect™ 96-well Cell Transformation Assay |
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| CBA-130-5 | Cell Biolabs | 5 x 96 assays | EUR 2280 |
CytoSelect 384-well Cell Transformation Assay, Fluorometric |
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| CBA-145 | Cell Biolabs | 384 assays | EUR 1208.4 |
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Description: Our CytoSelect 384-Well Cell Transformation Assay uses a modified soft agar 3D matrix to support the formation of colonies by neoplastic cells. Quantitation of cell transformation is performed on a fluorescence plate reader. |
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CytoSelect 384-well Cell Transformation Assay, Fluorometric |
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| CBA-145-5 | Cell Biolabs | 5 x 384 assays | EUR 4681.2 |
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Description: Our CytoSelect 384-Well Cell Transformation Assay uses a modified soft agar 3D matrix to support the formation of colonies by neoplastic cells. Quantitation of cell transformation is performed on a fluorescence plate reader. |
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293AD Cell Line |
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| AD-100 | Cell Biolabs | 1 vial | EUR 308 |
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Description: N/A |
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293AAV Cell Line |
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| AAV-100 | Cell Biolabs | 1 vial | EUR 340 |
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Description: N/A |
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293LTV Cell Line |
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| LTV-100 | Cell Biolabs | 1 vial | EUR 340 |
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Description: N/A |
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293RTV Cell Line |
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| RV-100 | Cell Biolabs | 1 vial | EUR 340 |
StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible) |
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| CBA-325 | Cell Biolabs | 96 assays | EUR 1027.2 |
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Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis. |
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StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible) |
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| CBA-325-5 | Cell Biolabs | 5 x 96 assays | EUR 4033.2 |
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Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis. |
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293/GFP Cell Line |
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| AKR-200 | Cell Biolabs | 1 vial | EUR 388 |
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Description: Fluorescence |
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T47D/GFP Cell Line |
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| AKR-208 | Cell Biolabs | 1 vial | EUR 686.4 |
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Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line. |
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A549/GFP Cell Line |
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| AKR-209 | Cell Biolabs | 1 vial | EUR 388 |
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Description: Fluorescence |
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HeLa/GFP Cell Line |
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| AKR-213 | Cell Biolabs | 1 vial | EUR 388 |
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Description: Fluorescence |
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293/Cas9 Cell Line |
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| AKR-5110 | Cell Biolabs | 1 vial | EUR 460 |
HeLa/Cas9 Cell Line |
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| AKR-5111 | Cell Biolabs | 1 vial | EUR 460 |
NIH3T3/GFP Cell Line |
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| AKR-214 | Cell Biolabs | 1 vial | EUR 388 |
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Description: Fluorescence |
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NIH3T3/Cas9 Cell Line |
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| AKR-5104 | Cell Biolabs | 1 vial | EUR 460 |
mAb Anti Human PD-L1 clone 12B4, Tracer (Biotin) (Monoclonal) |
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| MBS590085-01mL | MyBiosource | 0.1mL | EUR 565 |
mAb Anti Human PD-L1 clone 12B4, Tracer (Biotin) (Monoclonal) |
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| MBS590085-5x01mL | MyBiosource | 5x0.1mL | EUR 2290 |
mAb Anti-Human PD-1/CD279, 7G12, Tracer (Biotin) (Monoclonal |
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| MBS590084-01mL | MyBiosource | 0.1mL | EUR 565 |
mAb Anti-Human PD-1/CD279, 7G12, Tracer (Biotin) (Monoclonal |
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| MBS590084-5x01mL | MyBiosource | 5x0.1mL | EUR 2290 |
MCF-7/Luc Cell Line |
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| AKR-234 | Cell Biolabs | 1 vial | EUR 686.4 |
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Description: MCF-7/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line. |
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SKOV-3/Luc Cell Line |
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| AKR-232 | Cell Biolabs | 1 vial | EUR 686.4 |
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Description: SKOV-3/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line. |
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OVCAR-5/RFP Cell Line |
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| AKR-254 | Cell Biolabs | 1 vial | EUR 686.4 |
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Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line. |
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CytoSelect™ 96-Well Cell Transformation Assay (Soft Agar Colony Formation), Trial Size |
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| CBA-130-T | Cell Biolabs | 24 assays | EUR 320 |
Collagen-based Cell Contraction Assay |
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| CBA-201 | Cell Biolabs | 24 assays | EUR 348 |
Radius 384-Well Cell Migration Assay |
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| CBA-127 | Cell Biolabs | 384 assays | EUR 721.2 |
|
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap. |
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Radius 384-Well Cell Migration Assay |
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| CBA-127-5 | Cell Biolabs | 5 x 384 wells | EUR 2802 |
|
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap. |
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HIF-1 Alpha Cell Based ELISA Kit |
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| CBA-281 | Cell Biolabs | 96 assays | EUR 734.4 |
|
Description: Cell Biolabs? HIF-1 Cell Based ELISA Kit is an immunoassay developed for rapid detection of HIF-1 Alpha in fixed cells. Cells on a microplate are stimulated for HIF-1 Alpha stabilization, fixed, permeabilized, and then neutralized in the well. HIF-1 Alpha is then detected with an anti-HIF-1 alpha antibody followed by an HRP conjugated secondary antibody. Each kit provides sufficient reagents to perform up to a total of 96 assays and can detect HIF-1 Alpha from human, mouse, or rat. |
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CytoSelect™ MTT Cell Proliferation Assay |
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| CBA-252 | Cell Biolabs | 960 assays | EUR 268 |
Radius™ 24-Well Cell Migration Assay |
|||
| CBA-125 | Cell Biolabs | 24 assays | EUR 356 |
|
Description: Excepted Quantities |
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Radius™ 24-Well Cell Migration Assay |
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| CBA-125-5 | Cell Biolabs | 5 x 24 assays | EUR 1516 |
|
Description: Excepted Quantities |
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Radius™ 96-Well Cell Migration Assay |
|||
| CBA-126 | Cell Biolabs | 96 assays | EUR 416 |
|
Description: Excepted Quantities |
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Radius™ 96-Well Cell Migration Assay |
|||
| CBA-126-5 | Cell Biolabs | 5 x 96 assays | EUR 1788 |
|
Description: Excepted Quantities |
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Radius™ 48-Well Cell Migration Assay |
|||
| CBA-5037 | Cell Biolabs | 48 assays | EUR 372 |
|
Description: Excepted Quantities |
|||
Radius™ 48-Well Cell Migration Assay |
|||
| CBA-5037-5 | Cell Biolabs | 5 x 48 assays | EUR 1592 |
|
Description: Excepted Quantities |
|||
CytoSelect Cell Proliferation Assay Reagent (Fluorometric) |
|||
| CBA-250 | Cell Biolabs | 10 mL | EUR 490.8 |
|
Description: Cell Biolabs? CytoSelect Cell Proliferation Assay Reagent (Fluorometric) provides a fluorometric format for measuring and monitoring cell proliferation. Cells can be plated and then treated with compounds or agents that affect proliferation. Cells are then incubated with the proliferation reagent. Upon entering metabolically active live cells, the non-fluorescent proliferation reagent is converted into a bright red fluorescent form. An increase in cell proliferation is accompanied by increased fluorescent signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions. The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues. This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells. The kit contains sufficient reagents for the evaluation of 960 assays in ten 96-well plates or 192 assays in eight 24-well plates. |
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CytoSelect™ BrdU Cell Proliferation ELISA Kit |
|||
| CBA-251 | Cell Biolabs | 96 assays | EUR 380 |
|
Description: Excepted Quantities |
|||
CytoSelect™ Cell Viability and Cytotoxicity Assay |
|||
| CBA-240 | Cell Biolabs | 96 assays | EUR 256 |
Platinum-E Retroviral Packaging Cell Line, Ecotropic |
|||
| RV-101 | Cell Biolabs | 1 vial | EUR 648 |
Platinum-GP Retroviral Packaging Cell Line, Pantropic |
|||
| RV-103 | Cell Biolabs | 1 vial | EUR 648 |
CytoSelect 24-well Laminin Cell Invasion, Colorimetric |
|||
| CBA-110-LN | Cell Biolabs | 12 assays | EUR 714 |
|
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin. |
|||
Platinum-A Retroviral Packaging Cell Line, Amphotropic |
|||
| RV-102 | Cell Biolabs | 1 vial | EUR 648 |
CytoSelect™ 24-Well Cell Co-Culture System |
|||
| CBA-160 | Cell Biolabs | 24 assays | EUR 308 |
CytoSelect™ 24-Well Cell Co-Culture System |
|||
| CBA-160-5 | Cell Biolabs | 5 x 24 assays | EUR 1344 |
Total Protein - Murine Embryonic Stem Cell Line D3 |
|||
| CBA-305 | Cell Biolabs | 500 ?g | EUR 414 |
|
Description:
|
|||
CytoSelect™ 48-Well Cell Contraction Assay Kit |
|||
| CBA-5021 | Cell Biolabs | 48 assays | EUR 484 |
CytoSelect™ Cell Proliferation Assay Reagent (Colorimetric) |
|||
| CBA-253 | Cell Biolabs | 10 mL | EUR 268 |
|
Description: Colorimetric |
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Radius 24-Well Cell Migration Assay, (Laminin Coated) |
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| CBA-125-LN | Cell Biolabs | 24 assays | EUR 714 |
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Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap. |
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StemTAG PCR Primer Set for Stem Cell Characterization |
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| CBA-303 | Cell Biolabs | 1 kit | EUR 470.4 |
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Description: StemTAG PCR Primer Set for Stem Cell Characterization includes 7 primer pairs: Oct-4, NANOG, AFP, Flk-1, and NCAM, plus GAPDH and beta-actin as controls. |
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CytoSelect Clonogenic Tumor Cell Isolation Kit (5 preps) |
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| CBA-155 | Cell Biolabs | 5 preps | EUR 957.6 |
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Description: Many solid tumors contain heterogeneous populations of normal and cancerous cells. Separation of these cell populations is key to an accurate assessment of the true genotypic and phenotypic differences between normal and tumor cells. Our CytoSelect Clonogenic Tumor Cell Isolation Kit uses a proprietary semisolid agar medium to facilitate formation of colonies by cells from solid tumors. Colonies are grown in either a 6-well plate or a 35mm culture dish. These colonies are isolated away from single (i.e. normal) cells by size filtration. The viable cells from these colonies can be easily recovered for further analysis. |
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Radius 24-Well Cell Migration Assay, (Fibronectin Coated) |
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| CBA-125-FN | Cell Biolabs | 24 assays | EUR 714 |
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Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap. |
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CytoSelect™ 24-well Collagen Cell Invasion, Colorimetric |
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| CBA-110-COL | Cell Biolabs | 12 assays | EUR 448 |
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Description: Excepted Quantities |
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CytoSelect Proliferating Cell Nuclear Antigen (PCNA) ELISA Kit |
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| CBA-254 | Cell Biolabs | 96 assays | EUR 672 |
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Description: Cell Biolabs? CytoSelect Proliferating Cell Nuclear Antigen (PCNA) ELISA Kit is an enzyme immunoassay developed for the detection and quantitation of PCNA from nuclear and whole cell extracts. The kit detects PCNA from mouse, rat and human, and has a detection sensitivity limit of 12.5 ng/mLPCNA. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples. |
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Radius 24-Well Cell Migration Assay, (ECM Array Coated) |
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| CBA-125-ECM | Cell Biolabs | 24 wells | EUR 838.8 |
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Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap. |
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CytoSelect 48-well Cell Adhesion Assay (Laminin, Colorimetric) |
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| CBA-056 | Cell Biolabs | 48 assays | EUR 512.4 |
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Description: Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival. Our CytoSelect 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with Laminin. |
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CytoSelect 48-well Cell Adhesion Assay (Laminin, Fluorometric) |
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| CBA-057 | Cell Biolabs | 48 assays | EUR 553.2 |
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Description: Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival. Our CytoSelect 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with Laminin. |
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CytoSelect 24-well Laminin Cell Invasion Assay, Fluorometric |
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| CBA-111-LN | Cell Biolabs | 12 assays | EUR 714 |
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Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin. |
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CytoSelect 96-well Laminin Cell Invasion Assay, Fluorometric |
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| CBA-112-LN | Cell Biolabs | 96 assays | EUR 908.4 |
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Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin. |
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CytoSelect 48-well Cell Adhesion Assay (Fibrinogen, Colorimetric) |
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| CBA-058 | Cell Biolabs | 48 assays | EUR 512.4 |
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Description: Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival. Our CytoSelect 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with Fibrinogen. |
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CytoSelect 48-well Cell Adhesion Assay (Fibrinogen, Fluorometric) |
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| CBA-059 | Cell Biolabs | 48 assays | EUR 553.2 |
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Description: Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival. Our CytoSelect 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with Fibrinogen. |
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CytoSelect™ 96-well Phagocytosis Assay (Red Blood Cell) |
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| CBA-220 | Cell Biolabs | 96 assays | EUR 448 |
Radius™ 24-Well Cell Migration Assay, (Collagen I Coated) |
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| CBA-125-COL | Cell Biolabs | 24 assays | EUR 520 |
CytoSelect™ 24-well Collagen Cell Invasion Assay, Fluorometric |
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| CBA-111-COL | Cell Biolabs | 12 assays | EUR 448 |
CytoSelect™ 96-well Collagen Cell Invasion Assay, Fluorometric |
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| CBA-112-COL | Cell Biolabs | 96 assays | EUR 592 |
CytoSelect Clonogenic Tumor Cell Isolation Kit (5 x 5 preps) |
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| CBA-155-5 | Cell Biolabs | 25 preps | EUR 3894 |
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Description: Many solid tumors contain heterogeneous populations of normal and cancerous cells. Separation of these cell populations is key to an accurate assessment of the true genotypic and phenotypic differences between normal and tumor cells. Our CytoSelect Clonogenic Tumor Cell Isolation Kit uses a proprietary semisolid agar medium to facilitate formation of colonies by cells from solid tumors. Colonies are grown in either a 6-well plate or a 35mm culture dish. These colonies are isolated away from single (i.e. normal) cells by size filtration. The viable cells from these colonies can be easily recovered for further analysis. |
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CytoSelect™ 48-well Cell Adhesion Assay (Fibronectin, Colorimetric) |
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| CBA-050 | Cell Biolabs | 48 assays | EUR 308 |
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Description: Excepted Quantities |
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CytoSelect™ 48-well Cell Adhesion Assay (Fibronectin, Fluorometric) |
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| CBA-051 | Cell Biolabs | 48 assays | EUR 336 |
CytoSelect™ 24-well Cell Invasion (Basement Membrane), Fluorometric |
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| CBA-111 | Cell Biolabs | 12 assays | EUR 448 |
CytoSelect™ 24-well Cell Migration Assay (8 μm), Colorimetric |
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| CBA-100 | Cell Biolabs | 12 assays | EUR 404 |
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Description: Excepted Quantities |
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CytoSelect™ 24-well Cell Migration Assay (8 μm), Colorimetric |
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| CBA-100-5 | Cell Biolabs | 5 x 12 assays | EUR 1832 |
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Description: Excepted Quantities |
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CytoSelect™ 24-well Cell Migration Assay (8 μm), Fluorometric |
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| CBA-101 | Cell Biolabs | 12 assays | EUR 404 |
CytoSelect™ 24-well Cell Migration Assay (8 μm), Fluorometric |
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| CBA-101-5 | Cell Biolabs | 5 x 12 assays | EUR 1832 |
CytoSelect™ 24-well Cell Migration Assay (5 μm), Fluorometric |
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| CBA-102 | Cell Biolabs | 12 assays | EUR 404 |
CytoSelect™ 24-well Cell Migration Assay (5 μm), Fluorometric |
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| CBA-102-5 | Cell Biolabs | 5 x 12 assays | EUR 1832 |
CytoSelect™ 24-well Cell Migration Assay (3 μm), Fluorometric |
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| CBA-103 | Cell Biolabs | 12 assays | EUR 404 |
CytoSelect™ 24-well Cell Migration Assay (3 μm), Fluorometric |
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| CBA-103-5 | Cell Biolabs | 5 x 12 assays | EUR 1832 |
CytoSelect™ 96-well Cell Migration Assay (3 μm), Fluorometric |
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| CBA-104 | Cell Biolabs | 96 assays | EUR 488 |
CytoSelect™ 96-well Cell Migration Assay (3 μm), Fluorometric |
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| CBA-104-5 | Cell Biolabs | 5 x 96 assays | EUR 2132 |
CytoSelect™ 96-well Cell Migration Assay (5 μm), Fluorometric |
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| CBA-105 | Cell Biolabs | 96 assays | EUR 488 |
CytoSelect™ 96-well Cell Migration Assay (5 μm), Fluorometric |
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| CBA-105-5 | Cell Biolabs | 5 x 96 assays | EUR 2132 |
CytoSelect™ 96-well Cell Migration Assay (8 μm), Fluorometric |
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| CBA-106 | Cell Biolabs | 96 assays | EUR 488 |
CytoSelect™ 96-well Cell Migration Assay (8 μm), Fluorometric |
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| CBA-106-5 | Cell Biolabs | 5 x 96 assays | EUR 2132 |
CytoSelect™ 24-well Cell Migration Assay (12 μm), Colorimetric |
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| CBA-107 | Cell Biolabs | 12 assays | EUR 404 |
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Description: Excepted Quantities |
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CytoSelect™ 24-well Cell Migration Assay (12 μm), Fluorometric |
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| CBA-108 | Cell Biolabs | 12 assays | EUR 404 |
CytoSelect™ 48-well Cell Adhesion Assay (ECM Array, Colorimetric) |
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| CBA-070 | Cell Biolabs | 48 assays | EUR 432 |
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Description: Excepted Quantities |
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Since there’s a lack of NGF and its receptors within the AD forebrain, the speculation is that native supply of NGF to the nbM might revive the cholinergic circuitry and thereby restore cognitive features. Since NGF doesn’t move by the blood-brain barrier, approaches involving cerebral injections of genetically modified cells or viral vectors or implantation of encapsulated cells within the nbM in AD sufferers have been used. These makes an attempt have been partially profitable but additionally have limitations, that are introduced and mentioned right here. In conclusion, these trials level to the significance of additional growth of NGF-related therapies in AD.