A universal gene correction approach for FKRP-associated dystroglycanopathies to enable autologous cell therapy

A universal gene correction approach for FKRP-associated dystroglycanopathies to enable autologous cell therapy

Mutations within the fukutin-related protein (FKRP) gene end in a broad spectrum of muscular dystrophy (MD) phenotypes, together with the extreme Walker-Warburg syndrome (WWS). Right here, we develop a gene-editing method that replaces your complete mutant open studying body with the wild-type sequence to universally right all FKRP mutations.
We apply this method to right FKRP mutations in induced pluripotent stem (iPS) cells derived from sufferers displaying broad medical severity. Our findings present rescue of useful α-dystroglycan (α-DG) glycosylation in gene-edited WWS iPS cell-derived myotubes. Transplantation of gene-corrected myogenic progenitors within the FKRPP448L-NSG mouse mannequin offers rise to myofiber and satellite tv for pc cell engraftment and, importantly, restoration of α-DG useful glycosylation in vivo. These findings counsel the potential feasibility of utilizing CRISPR-Cas9 expertise together with patient-specific iPS cells for the long run growth of autologous cell transplantation for FKRP-associated MDs.

An replace on Alzheimer’s illness: Immunotherapeutic brokers, stem cell remedy and gene enhancing

Alzheimer’s illness is a persistent way of life ailment whose incidence has come to gentle with the rising life expectancy because of higher healthcare. The affected person burden for AD is about to double by the yr 2060 and development in analysis is of utmost significance to fight this downside. AD is characterised by the pathological hallmarks of amyloid plaques and neurofibrillary tangles.
The illness has been implicated to have a genetic predisposition. The present therapy methods are at finest ameliorative in nature and provide no substantive remedy. Immunotherapeutic approaches employed have proven few therapeutic advantages however the accelerated approval of aducanumab by the US-FDA reveals medical profit benefit. As well as, newer therapeutic approaches are the necessity of the hour. This evaluation goals to focus on the pathology of the illness, adopted by an perception into newer approaches like stem cell remedy and gene enhancing, specializing in potential CRISPR mediated targets.

An outline of cell and gene remedy growth in China

China, the primary nation worldwide to approve a gene remedy in 2003, nearly misplaced the benefit for a head begin in cell and gene remedy (CGT) growth because of a scarcity of clear and strict regulatory frameworks. The speedy developments of CGTs’ growth worldwide in addition to their therapeutic potential have triggered the federal government to conduct a spate of regulatory reforms as a way to promote normative growth of CGTs in China.
Inspired by coverage assist, the outstanding progress for CGTs in China has been noticed over the previous few years, thereby catapulting China again into the forefront of CGTs worldwide. This manuscript goals to offer an outline of regulatory reforms, the present growth panorama of CGTs, in addition to key contributors and challenges for CGT growth in China.
 A universal gene correction approach for FKRP-associated dystroglycanopathies to enable autologous cell therapy

Gene Remedy for Sickle Cell Illness – Transferring from the Bench to the Bedside

Gene remedy as a possible remedy for sickle cell illness (SCD) has lengthy been pursued provided that this hemoglobin dysfunction outcomes from a single level mutation. Advances in genomic sequencing, elevated understanding of hemoglobin regulation and discoveries of molecular instruments for genome modification of hematopoietic stem cells have made gene remedy for SCD potential. Gene addition methods utilizing gene switch vectors have been optimized over the previous couple of many years to allow expression of regular or anti-sickling globins as methods to ameliorate SCD.
Many hurdles needed to be addressed previous to medical translation together with assortment of ample stem cells for gene-modification, rising expression of transferred genes to a therapeutic degree and conditioning sufferers in a protected method that enabled enough engraftment of gene-modified cells. The invention of genome editors that make exact modifications has additional superior the protection and efficacy of gene remedy and a speedy motion to medical trial has undoubtedly been supported by classes realized from optimizing gene addition methods.
Present gene therapies being examined in medical trial require important infrastructure and experience given the wants to reap cells from and administer chemotherapy to sufferers who usually have important organ dysfunction and that gene-modification takes place ex vivo in specialised amenities. For these therapies to appreciate their full potential they might have to be transportable, protected and environment friendly making an in-vivo primarily based method engaging. Moreover, enough assets for SCD screening and entry to standardized care are critically necessary for gene remedy to be a viable therapy possibility for SCD.

Gene and cell remedy for the nucleus basalis of Meynert with NGF in Alzheimer’s illness

There may be at present no efficient therapy for the most typical of the dementia issues, Alzheimer’s illness (AD). It has been recognized for many years that the central cholinergic system is necessary for reminiscence. The cholinergic neurons within the basal forebrain with its cortical and hippocampal projections degenerate in AD and thus contribute to the cognitive decline attribute of AD.
This data led to the event of the at present permitted therapy for AD, with inhibitors of acetylcholine-esterase concentrating on the cholinergic system with useful however gentle results. Lately, different approaches to affect the degenerating cholinergic system in AD specializing in nerve development issue (NGF) have been undertaken. NGF is required for the survival and performance of the basal forebrain cholinergic neurons, an important being the nucleus basalis of Meynert (nbM).

MemBriteâ„¢ Fix 568/580

30095 1KIT
EUR 772.8
Description: Minimum order quantity: 1 unit of 1KIT

PTPN11 (Ab-580) Antibody

CSB-PA961746-
EUR 402
Description: A polyclonal antibody against PTPN11 (Ab-580). Recognizes PTPN11 (Ab-580) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200

PTPN11 (Ab-580) Antibody

CSB-PA961746-100ul 100ul
EUR 379.2
Description: A polyclonal antibody against PTPN11 (Ab-580). Recognizes PTPN11 (Ab-580) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200

hsa-miR-580 Primers

MPH01878 150 ul / 10 uM
EUR 145.2

PYK2 (Phospho-Tyr579/580) Antibody

13230-100ul 100ul
EUR 302.4

PYK2 (Phospho-Tyr579/580) Antibody

13230-50ul 50ul
EUR 224.4

SHP-2 (Ab-580) Antibody

21320-100ul 100ul
EUR 302.4

SHP-2 (Ab-580) Antibody

21320-50ul 50ul
EUR 224.4

hsa-miR-580-3p Primers

MPH03331 150 ul / 10 uM
EUR 145.2

hsa-miR-580-5p Primers

MPH03332 150 ul / 10 uM
EUR 145.2

hsa-miR-580 miRNA Antagomir

MNH03164 2 x 5.0 nmol
EUR 394.8

hsa-miR-580 miRNA Inhibitor

MIH03164 2 x 5.0 nmol
EUR 211.2

anti-SHP-2 (Ab-580)

LF-PA20454 100 ul
EUR 400.8
Description: Rabbit polyclonal to SHP-2

mFluor™ Blue 580 SE

1178 1 mg
EUR 318

DIPAK LONG

CPDI000L1 EACH
EUR 304.62

Serpin A1c Recombinant Protein

91-580 0.05 mg
EUR 821.4
Description: Alpha-1-antitrypsin 1-3(SERPIN A1) is a secreted protein and belongs to the serpin family. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. Mouse SERPIN A1 is a serine protease inhibitor whose targets include elastase,plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema orliver disease. Several transcript variants encoding the same protein have been found for this gene.

Kallikrein 7 Recombinant Protein

92-580 0.05 mg
EUR 821.4
Description: Kallikrein7, also named as stratum corneum chymotryptic enzyme (SCCE), is a secreted protein of the Kallikrein-related peptidase (KLK) family. This family contains fifteen homologous secreted serine endopeptidases and plays a significant role in various physiological processes, including skin desquamation, semen liquefaction, neural plasticity, and body fluid homeostasis. In skin KLK5, KLK 7 and KLK14 are able to degrade corneodesmosomes, which leads to desquamation of skin surface cells. KLK activation is believed to be mediated through highly organized proteolytic cascades, regulated through a series of feedback loops, inhibitors, auto-degradation and internal cleavages. Studies have shown that one potential physiological activator for KLK7 is KLK5. Along with KLK14, these three kallikreins form a proteolytic cascade in the stratum corneum. KLK7 is primarily expressed in the skin but is also detected at relatively high levels in esophagus, heart, liver, central nervous system, kidney, pancreas, mammary and salivary glands.

Mesothelin / MSLN (296-580) Recombinant Protein

11-216 0.1 mg
EUR 714.3
Description: Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.

DAX-J2™ Ratio 580/460

16310 1 mg
EUR 471.6

MemBriteâ„¢ Fix 568/580, Trial Size

30095-T 1KIT
EUR 266.4
Description: Minimum order quantity: 1 unit of 1KIT

Zinc Finger Protein 580 (ZNF580) Antibody

abx030431-400ul 400 ul
EUR 627.6

Zinc Finger Protein 580 (ZNF580) Antibody

abx030431-80l 80 µl
EUR 343.2

Phospho-PYK2 (Tyr579/580) Blocking Peptide

AF7383-BP 1mg
EUR 234

PYK2 (Phospho-Tyr579/580) Conjugated Antibody

C13230 100ul
EUR 476.4

hsa-miR-580-3p miRNA Antagomir

MNH03165 2 x 5.0 nmol
EUR 394.8

hsa-miR-580-5p miRNA Antagomir

MNH03166 2 x 5.0 nmol
EUR 394.8

hsa-miR-580-3p miRNA Inhibitor

MIH03165 2 x 5.0 nmol
EUR 211.2

hsa-miR-580-5p miRNA Inhibitor

MIH03166 2 x 5.0 nmol
EUR 211.2

Recombinant human Zinc finger protein 580

P2142 100ug Ask for price
Description: Recombinant protein for human Zinc finger protein 580

Long trebler phosphoramidite

A8151-250 250 mg
EUR 408
Description: Long trebler phosphoramidite is a branching reagent for oligonucleotide synthesis allowing to synthesize branched DNA structures using standard DNA synthesizer.

Wick 100mm Long

BUR4508 PK10
EUR 6.27

CytoFix™ BCECF, AM *Optimized for long term cellular pH tracking*

21200 1 mg
EUR 318

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric

CBA-135 96 assays
EUR 985.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric

CBA-135-5 5 x 96 assays
EUR 4027.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric

CBA-140 96 assays
EUR 1027.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric

CBA-140-5 5 x 96 assays
EUR 4179.6
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

Rabbit B Cell Leukemia X long ELISA kit

E04B0575-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rabbit B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit B Cell Leukemia X long ELISA kit

E04B0575-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rabbit B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit B Cell Leukemia X long ELISA kit

E04B0575-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rabbit B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig B Cell Leukemia X long ELISA kit

E07B0575-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Porcine B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig B Cell Leukemia X long ELISA kit

E07B0575-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Porcine B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig B Cell Leukemia X long ELISA kit

E07B0575-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Porcine B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human B Cell Leukemia X long ELISA kit

E01B0575-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human B Cell Leukemia X long ELISA kit

E01B0575-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human B Cell Leukemia X long ELISA kit

E01B0575-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat B Cell Leukemia X long ELISA kit

E06B0575-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat B Cell Leukemia X long ELISA kit

E06B0575-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Goat B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat B Cell Leukemia X long ELISA kit

E06B0575-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Goat B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse B Cell Leukemia X long ELISA kit

E03B0575-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse B Cell Leukemia X long ELISA kit

E03B0575-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse B Cell Leukemia X long ELISA kit

E03B0575-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog B Cell Leukemia X long ELISA kit

E08B0575-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog B Cell Leukemia X long ELISA kit

E08B0575-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog B Cell Leukemia X long ELISA kit

E08B0575-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat B Cell Leukemia X long ELISA kit

E02B0575-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat B Cell Leukemia X long ELISA kit

E02B0575-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat B Cell Leukemia X long ELISA kit

E02B0575-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey B Cell Leukemia X long ELISA kit

E09B0575-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Monkey B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey B Cell Leukemia X long ELISA kit

E09B0575-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Monkey B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey B Cell Leukemia X long ELISA kit

E09B0575-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Monkey B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Nunc Cell Factory PC connector none sterile long

140099 PK10
EUR 287.28

293AAV Cell Line

AAV-100 1 vial
EUR 609.6
Description: The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production, including firmer attachment and larger surface area.

293AD Cell Line

AD-100 1 vial
EUR 553.2
Description: The 293AD Cell Line is derived from the parental 293 cells but selected for attributes that increase adenovirus production, including firmer attachment and larger surface area.

293LTV Cell Line

LTV-100 1 vial
EUR 609.6
Description: The 293LTV Cell Line is derived from the parental 293 cells but selected for attributes that increase lentiviral production, including fimrer attachment and larger surface area.

293RTV Cell Line

RV-100 1 vial
EUR 609.6
Description: The 293RTV Cell Line is derived from the parental 293 cells but selected for attributes that increase retroviral production, including fimrer attachment and larger surface area.

Human Zinc finger protein 580, ZNF580 ELISA KIT

ELI-51274h 96 Tests
EUR 988.8

Mouse Zinc finger protein 580, Znf580 ELISA KIT

ELI-51275m 96 Tests
EUR 1038

Human pre-microRNA Expression Construct Lenti-miR-580

PMIRH580PA-1 Bacterial Streak
EUR 820.8

Human MSLN Protein (Glu 296-Gly 580) [Fc]

VAng-2562Lsx-100g 100 µg
EUR 984
Description: Human Mesothelin protein, human IgG1 Fc tag, was expressed in human 293 cells. (Uniprot ID: AAH09272)

Human MSLN Protein (Glu 296-Gly 580) [Fc]

VAng-2562Lsx-1mg 1 mg
EUR 5998.8
Description: Human Mesothelin protein, human IgG1 Fc tag, was expressed in human 293 cells. (Uniprot ID: AAH09272)

Human MSLN Protein (Glu 296-Gly 580) [His]

VAng-2563Lsx-100g 100 µg
EUR 1099.2
Description: Human Mesothelin protein, His tag, was expressed in human 293 cells. (Uniprot ID: AAH09272)

Human MSLN Protein (Glu 296-Gly 580) [His]

VAng-2563Lsx-1mg 1 mg
EUR 6592.8
Description: Human Mesothelin protein, His tag, was expressed in human 293 cells. (Uniprot ID: AAH09272)

Recombinant Pseudomonas Aeruginosa PA14_21660 Protein (aa 1-580)

VAng-Cr0847-1mgEcoli 1 mg (E. coli)
EUR 8961.6
Description: Pseudomonas Aeruginosa (strain UCBPP-PA14) UPF0060 membrane protein PA14_21660, recombinant protein.

Recombinant Pseudomonas Aeruginosa PA14_21660 Protein (aa 1-580)

VAng-Cr0847-500gEcoli 500 µg (E. coli)
EUR 6040.8
Description: Pseudomonas Aeruginosa (strain UCBPP-PA14) UPF0060 membrane protein PA14_21660, recombinant protein.

Recombinant Pseudomonas Aeruginosa PA14_21660 Protein (aa 1-580)

VAng-Cr0847-50gEcoli 50 µg (E. coli)
EUR 4104
Description: Pseudomonas Aeruginosa (strain UCBPP-PA14) UPF0060 membrane protein PA14_21660, recombinant protein.

Recombinant Toxoplasma Gondii MIC4 Protein (aa 1-580)

VAng-0439Lsx-inquire inquire Ask for price
Description: Toxoplasma Gondii Micronemal protein 4, recombinant protein.

Recombinant Brucella Abortus fliF Protein (aa 1-580)

VAng-Lsx4789-inquire inquire Ask for price
Description: Brucella Abortus biovar 1 (strain 9-941) Flagellar M-ring protein, recombinant protein.

Recombinant Mycobacterium Paratuberculosis mhpA Protein (aa 1-580)

VAng-Yyj0966-inquire inquire Ask for price
Description: Mycobacterium Paratuberculosis 3- (3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase (mhpA), recombinant protein.

Recombinant Mycobacterium Avium mhpA Protein(aa 1-580)

VAng-Yyj1323-inquire inquire Ask for price
Description: Mycobacterium Avium (strain 104) 3- (3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase (mhpA), recombinant protein.

Recombinant Mycobacterium Marinum fadD28 Protein (aa 1-580)

VAng-Yyj4351-inquire inquire Ask for price
Description: Mycobacterium Marinum (strain ATCC BAA-535 / M) Long-chain-fatty-acid--AMP ligase FadD28 (fadD28), recombinant protein.

Recombinant Mycobacterium Avium mhpA Protein (aa 1-580)

VAng-Yyj2652-inquire inquire Ask for price
Description: Mycobacterium Avium (strain 104) 3- (3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase (mhpA), recombinant protein.

Recombinant Mycobacterium Abscessus proS Protein (aa 1-580)

VAng-Yyj5520-inquire inquire Ask for price
Description: Mycobacterium Abscessus (strain ATCC 19977 / DSM 44196) Proline--tRNA ligase (proS), recombinant protein.

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)

CBA-325 96 assays
EUR 1027.2
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)

CBA-325-5 5 x 96 assays
EUR 4033.2
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

RSV Long Strain Protein

abx060644-100ug 100 ug
EUR 1161.6

RSV (Strain Long) Protein

abx069909-1ml 1 ml
EUR 393.6

RSV (Long Strain) Protein

abx069910-1mg 1 mg
EUR 2498.4

DISC1-long-specific Antibody

abx232397-100ug 100 ug
EUR 610.8

Pasteur pipette 5.75in long

CLS7095B5X-1000EA PK1000
EUR 59.28

Long Feather scalpel n11

DD530038 PK20
EUR 53.58

Bend Long 1019 Interchange

GLA3090 EACH
EUR 13.11

Bend Long 1423 Interchange

GLA3092 EACH
EUR 13.11

Bend Long 1926 Interchange

GLA3094 EACH
EUR 13.11

Bend Long 2429 Interchange

GLA3096 EACH
EUR 13.57

Bend Long 2932 Interchange

GLA3098 EACH
EUR 13.45

Pateur glass long plugged

PIP4106 PK250
EUR 106.75

PROGARD 2S ALONE (LONG)

PR0G000S2 EACH
EUR 456

Electroporation Cuvettes 1mm Long

FBR-201 PK50
EUR 121.98

Electroporation Cuvettes 2mm Long

FBR-202 PK50
EUR 119.7

Electroporation Cuvettes 4mm Long

FBR-204 PK50
EUR 121.98

Kinesis VacuTight Headless Long

CHR4571 EACH
EUR 5.84

Tongs Furnace 460mm Long

TON1104 EACH
EUR 157.32

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric, Trial Size

CBA-135-T 24 assays
EUR 518.4
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

293/GFP Cell Line

AKR-200 1 vial
EUR 686.4
Description: 293/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

T47D/GFP Cell Line

AKR-208 1 vial
EUR 686.4
Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

A549/GFP Cell Line

AKR-209 1 vial
EUR 686.4
Description: A549/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

HeLa/GFP Cell Line

AKR-213 1 vial
EUR 686.4
Description: HeLa/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/GFP Cell Line

AKR-214 1 vial
EUR 686.4
Description: NIH3T3/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/Cas9 Cell Line

AKR-5104 1 vial
EUR 686.4

293/Cas9 Cell Line

AKR-5110 1 vial
EUR 686.4

HeLa/Cas9 Cell Line

AKR-5111 1 vial
EUR 686.4

CytoSelect 96-Well Cell Transformation Assay (Cell Recovery Compatible, Fluorometric), Trial Size

CBA-140-T 24 assays
EUR 547.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

SKOV-3/Luc Cell Line

AKR-232 1 vial
EUR 686.4
Description: SKOV-3/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

MCF-7/Luc Cell Line

AKR-234 1 vial
EUR 686.4
Description: MCF-7/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

OVCAR-5/RFP Cell Line

AKR-254 1 vial
EUR 686.4
Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line.

Collagen-based Cell Contraction Assay

CBA-201 24 assays
EUR 582
Description: Cell Biolabs? Collagen-based Contraction Assay Kit provides a simple system to assess cell contractivity in vitro and screen cell contraction mediators. Each kit provides sufficient quantities to perform up to 24 assays in a 24-well plate. The kit can be also used in culturing cells in 3D collagen matrix.

CytoSelect MTT Cell Proliferation Assay

CBA-252 960 assays
EUR 490.8
Description: Cell Biolabs? CytoSelect MTT Cell Proliferation Assay provides a colorimetric format for measuring and monitoring cell proliferation.  The kit contains sufficient reagents for the evaluation of 960 assays in 96-well plates or 192 assays in 24-well plates.  Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then detected with the proliferation reagent, which is converted in live cells from the yellow tetrazole MTT to the purple formazan form by a cellular reductase (Figure 1).  An increase in cell proliferation is accompanied by an increased signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells.

Guinea pig B Cell Leukemia X long ELISA kit

E05B0575-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Guinea pig B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig B Cell Leukemia X long ELISA kit

E05B0575-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Guinea pig B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig B Cell Leukemia X long ELISA kit

E05B0575-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Guinea pig B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Biotinylated Human MSLN Protein (Glu 296-Gly 580) [Fc]

VAng-2560Lsx-200g 200 µg
EUR 4184.4
Description: Biotinylated Human Mesothelin protein, human IgG1 Fc tag, was expressed in human 293 cells. (Uniprot ID: AAH09272.1)

Biotinylated Human MSLN Protein (Glu 296-Gly 580) [Fc]

VAng-2560Lsx-25g 25 µg
EUR 1017.6
Description: Biotinylated Human Mesothelin protein, human IgG1 Fc tag, was expressed in human 293 cells. (Uniprot ID: AAH09272.1)

Biotinylated Human MSLN Protein (Glu 296-Gly 580) [His]

VAng-2561Lsx-200g 200 µg
EUR 4184.4
Description: Biotinylated Human Mesothelin protein, His tag, was expressed in human 293 cells. (Uniprot ID: AAH09272.1)

Biotinylated Human MSLN Protein (Glu 296-Gly 580) [His]

VAng-2561Lsx-25g 25 µg
EUR 1017.6
Description: Biotinylated Human Mesothelin protein, His tag, was expressed in human 293 cells. (Uniprot ID: AAH09272.1)

Recombinant C1q R1 Protein (Thr 22-Lys 580) [His]

VAng-1141Lsx-1mg 1 mg
EUR 5998.8
Description: Human C1q R1 (CD93), His tag, is expressed in HEK 293 cells. (Uniprot ID: AAH28075)

Recombinant C1q R1 Protein (Thr 22-Lys 580) [His]

VAng-1141Lsx-50g 50 µg
EUR 1017.6
Description: Human C1q R1 (CD93), His tag, is expressed in HEK 293 cells. (Uniprot ID: AAH28075)

Recombinant Plasmodium Falciparum MSP-1 Protein (aa 200-580)

VAng-Wyb6594-1mgEcoli 1 mg (E. coli)
EUR 5536.8
Description: Plasmodium Falciparum (isolate Wellcome) Merozoite surface protein 1, recombinant protein.

Recombinant Plasmodium Falciparum MSP-1 Protein (aa 200-580)

VAng-Wyb6594-500gEcoli 500 µg (E. coli)
EUR 3921.6
Description: Plasmodium Falciparum (isolate Wellcome) Merozoite surface protein 1, recombinant protein.

Recombinant Plasmodium Falciparum MSP-1 Protein (aa 200-580)

VAng-Wyb6594-50gEcoli 50 µg (E. coli)
EUR 2683.2
Description: Plasmodium Falciparum (isolate Wellcome) Merozoite surface protein 1, recombinant protein.

CytoSelect 24-well Cell Invasion, Fluorometric

CBA-111 12 assays
EUR 714
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with ECM matrix gel (basement membrane), a protein mix isolated from EHS tumor cells.

CytoSelect 96-well Cell Invasion, Fluorometric

CBA-112 96 assays
EUR 908.4
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Basement Membrane, an ECM protein mix isolated from EHS tumor cells.

Radius 24-Well Cell Migration Assay

CBA-125 24 assays
EUR 602.4
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 24-Well Cell Migration Assay

CBA-125-5 5 x 24 assays
EUR 2362.8
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 96-Well Cell Migration Assay

CBA-126 96 assays
EUR 686.4
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 96-Well Cell Migration Assay

CBA-126-5 5 x 96 assays
EUR 2697.6
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 384-Well Cell Migration Assay

CBA-127 384 assays
EUR 721.2
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 384-Well Cell Migration Assay

CBA-127-5 5 x 384 wells
EUR 2802
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

CytoSelect 96-well Cell Transformation Assay

CBA-130 96 assays
EUR 866.4
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

CytoSelect 96-well Cell Transformation Assay

CBA-130-5 5 x 96 assays
EUR 3463.2
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

CytoSelect Cell Viability and Cytotoxicity Assay

CBA-240 96 assays
EUR 470.4
Description: The CytoSelect Cell Viability and Cytotoxicity Assay Kit provides a simple format for monitoring cell viability via metabolic activity. Live cells are detected with either MTT (colorimetric detection) or Calcein AM (fluorometric detection). Dead cells are detected by EthD-1 reagent (fluorometric). All 3 detection reagents are included, along with Saponin (a cell death initiator). Prior to the assay, cells may be treated with compounds or agents that affect cell viability. This kit is suitable for eukaryotic cells, not yeast or bacteria.

CytoSelect Cell Proliferation Assay Reagent (Fluorometric)

CBA-250 10 mL
EUR 490.8
Description: Cell Biolabs? CytoSelect Cell Proliferation Assay Reagent (Fluorometric) provides a fluorometric format for measuring and monitoring cell proliferation. Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then incubated with the proliferation reagent.  Upon entering metabolically active live cells, the non-fluorescent proliferation reagent is converted into a bright red fluorescent form. An increase in cell proliferation is accompanied by increased fluorescent signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells. The kit contains sufficient reagents for the evaluation of 960 assays in ten 96-well plates or 192 assays in eight 24-well plates.

CytoSelect BrdU Cell Proliferation ELISA Kit

CBA-251 96 assays
EUR 637.2
Description: The CytoSelect BrdU Cell Proliferation ELISA Kit detects BrdU incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody.  When cells are incubated in media containing BrdU, the pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells.  Once the labeling media is removed, the cells are fixed and the DNA is denatured in one step with a fix/denature solution (denaturation of the DNA is necessary to improve the accessibility of the incorporated BrdU for detection).  Then an anti-BrdU mouse monoclonal antibody is added followed by an HRP conjugated secondary antibody to detect the incorporated BrdU.  The magnitude of the absorbance for the developed color is proportional to the quantity of BrdU incorporated into cells and can be directly correlated to cell proliferation.

CytoSelect Cell Proliferation Assay Reagent (Colorimetric)

CBA-253 10 mL
EUR 490.8
Description: Cell Biolabs? CytoSelect WST-1 Cell Proliferation Assay Reagent provides a colorimetric format for measuring and monitoring cell proliferation.  The 10 mL volume is sufficient for the evaluation of 960 assays in ten 96-well plates or 192 assays in eight 24-well plates.  Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then detected with the proliferation reagent, which is converted in live cells from WST-1 to the formazan form in the presence of cellular NADH and an electron mediator. An increase in cell proliferation is accompanied by increased signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells.

Radius 48-Well Cell Migration Assay

CBA-5037 48 assays
EUR 622.8
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 48-Well Cell Migration Assay

CBA-5037-5 5 x 48 assays
EUR 2454
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

CytoSelect 24-well Cell Invasion Assay, Colorimetric

CBA-110 12 assays
EUR 714
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with ECM matrix gel (basement membrane), a protein mix isolated from EHS tumor cells.

CytoSelect 24-well Collagen Cell Invasion, Colorimetric

CBA-110-COL 12 assays
EUR 714
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with Collagen I.

CytoSelect 24-well Laminin Cell Invasion, Colorimetric

CBA-110-LN 12 assays
EUR 714
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin.

CytoSelect 384-well Cell Transformation Assay, Fluorometric

CBA-145 384 assays
EUR 1208.4
Description: Our CytoSelect 384-Well Cell Transformation Assay uses a modified soft agar 3D matrix to support the formation of colonies by neoplastic cells. Quantitation of cell transformation is performed on a fluorescence plate reader.

CytoSelect 384-well Cell Transformation Assay, Fluorometric

CBA-145-5 5 x 384 assays
EUR 4681.2
Description: Our CytoSelect 384-Well Cell Transformation Assay uses a modified soft agar 3D matrix to support the formation of colonies by neoplastic cells. Quantitation of cell transformation is performed on a fluorescence plate reader.

CytoSelect 24-Well Cell Co-Culture System

CBA-160 24 assays
EUR 525.6
Description: CytoSelect 24-Well Cell Co-Culture System provides a unique platform to monitor direct contact between two cell types in a single well. First, cells are cultured until they form a monolayer around the insert, creating a defined 8 mm cell-free zone. Once the insert is removed, a second cell type may be seeded into the exposed zone. The kit contains proprietary treated inserts and sufficient reagents for the evaluation of 24 samples. The inserts are compatible with most adherent cell types and experimental conditions.

CytoSelect 24-Well Cell Co-Culture System

CBA-160-5 5 x 24 assays
EUR 2078.4
Description: CytoSelect 24-Well Cell Co-Culture System provides a unique platform to monitor direct contact between two cell types in a single well. First, cells are cultured until they form a monolayer around the insert, creating a defined 8 mm cell-free zone. Once the insert is removed, a second cell type may be seeded into the exposed zone. The kit contains proprietary treated inserts and sufficient reagents for the evaluation of 24 samples. The inserts are compatible with most adherent cell types and experimental conditions.

HIF-1 Alpha Cell Based ELISA Kit

CBA-281 96 assays
EUR 734.4
Description: Cell Biolabs? HIF-1 Cell Based ELISA Kit is an immunoassay developed for rapid detection of HIF-1 Alpha in fixed cells. Cells on a microplate are stimulated for HIF-1 Alpha stabilization, fixed, permeabilized, and then neutralized in the well. HIF-1 Alpha is then detected with an anti-HIF-1 alpha antibody followed by an HRP conjugated secondary antibody. Each kit provides sufficient reagents to perform up to a total of 96 assays and can detect HIF-1 Alpha from human, mouse, or rat.

CytoSelect 48-Well Cell Contraction Assay Kit

CBA-5021 48 assays
EUR 762
Description: Cell Biolabs? Cell Contraction Assays (Floating Matrix Model) provide a simple, in vitro system to assess cell contractivity and screen cell contraction mediators. The proprietary Cell Contraction Plate eliminates the matrix releasing step of the conventional contraction assay, providing a faster, higher-throughput method to assess cell contraction.

Platinum-E Retroviral Packaging Cell Line, Ecotropic

RV-101 1 vial
EUR 1104
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-E cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Platinum-A Retroviral Packaging Cell Line, Amphotropic

RV-102 1 vial
EUR 1104
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-A cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Platinum-GP Retroviral Packaging Cell Line, Pantropic

RV-103 1 vial
EUR 1104
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-GP cells contain the gag and pol genes required for retroviral packaging; an expression vector is co-transfected with a VSVG envelope vector.

Long trebler phosphoramidite, 1 g

44260 1 g
EUR 418.8

Isopeptidase T protein (long form)

30R-2739 25 ug
EUR 429.6
Description: Purified recombinant Human Isopeptidase T protein (long form)

Long trebler phosphoramidite, 500 mg

34260 500 mg
EUR 259.2

Long trebler phosphoramidite, 250 mg

24260 250 mg
EUR 178.8
Since there’s a lack of NGF and its receptors within the AD forebrain, the speculation is that native supply of NGF to the nbM might revive the cholinergic circuitry and thereby restore cognitive features. Since NGF doesn’t move by the blood-brain barrier, approaches involving cerebral injections of genetically modified cells or viral vectors or implantation of encapsulated cells within the nbM in AD sufferers have been used. These makes an attempt have been partially profitable but additionally have limitations, that are introduced and mentioned right here. In conclusion, these trials level to the significance of additional growth of NGF-related therapies in AD.

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