Nova Lifetech / Life Science Market plasmids and vectors
Genome engineering of non-conventional microorganisms requires the event of devoted artificial biology instruments. Pseudomonas putida is a Gram-negative, non-pathogenic soil bacterium extensively used for metabolic engineering owing to its versatile metabolism and excessive ranges of tolerance to various kinds of stress. Genome enhancing of P. putida largely depends on homologous recombination occasions, assisted by helper plasmid-based expression of genes encoding […]
With the rise of recent highly effective genome engineering applied sciences, equivalent to CRISPR/Cas9, cell fashions might be engineered successfully to speed up fundamental and illness analysis. Essentially the most essential step on this process is the environment friendly supply of international nucleic acids into cells by mobile transfection. For the reason that vectors encoding […]
The supercoiled round (SC) topology type of plasmid DNA has been regarded to be advantageous over open round or linearized analogue in transfection and expression effectivity, and due to this fact are largely demanded within the biopharmaceutical manufacturing. Nevertheless, manufacturing of high-purity SC plasmid DNA would lead to excessive manufacturing value. The impact of SC […]
In our earlier research, a novel gene remedy method was developed based mostly on a plasmid vector pSecTag2B through which recombinant HNP1 gene was regulated below a cytomegalovirus promoter to encode a mature HNP1 type. We confirmed for the primary time in numerous tumor fashions together with human most cancers xenografts that overexpression of HNP1 […]